scholarly journals Purification and bioactivity of fucoidan from kappaphycus alvarezii alga

2019 ◽  
Vol 57 (3B) ◽  
pp. 59
Author(s):  
Nhon Thi Ngoc Hoang ◽  
Anh Thi Hong Le ◽  
Kiet Tuan Nguyen ◽  
Chi Thi Minh Nguyen ◽  
Ngoc Thi My Le ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antioxidant Activity ◽  
Biological Activities ◽  
Kappaphycus Alvarezii ◽  
Antibacterial Activities ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Dpph Assay ◽  
Coagulation Time ◽  
Coast Of Vietnam

Kappaphycus alvarezii is a type of seaweed containing numerous carbohydrate with biological activities, especially fucoidan. About chemical aspect, fucoidan is fucose polysaccharides (FCSPs) with diverse biological activities including antioxidant, anticoagulant, anti-inflammatory, antiviral, antitumor... Fucoidan is found not only in brown algae but also in K. alvarezii alga from the central coast of Vietnam. In this study, fucoidan purification, fucoidan structure and biological activities of K. alvarezii alga (collected at Dam Mon area, Khanh Hoa province) were investigated. The yield recovery of two-stage precipitation by ethanol 99% was 84.4%. The protocol of fucoidan purification by ion-exchange chromatography was applied with tris-HCl buffer, elution solvent NaCl 1M; The yield was 60.99%. The structure of fucoidan from K. alvarezii was determined by 1H-NMN spectrum with the specific characteristic properties of fucoidan. The antioxidant activity of the purified fucoidan via IC50 was 303.51±0.12 μg/mL (DPPH assay), 299.97±3.12 μg/mL (ABTS essay). The purified fucoidan was shown better ability in cases of anti-coagulant, antibacterial activities than that of the crude extract. The anti-coagulation time of the purified fucoidan was 22.52 minutes. The minimum inhibitory concentrations (MICs) of the antibacterial activity (Bacillus cereus, Escherichia coli) were 400μg/mL. MICs was 500 μg/mL (Aspergillus flavus) and 600 μg/mL (Aspergillus niger).

scholarly journals Thymus mastichina: Composition and Biological Properties with a Focus on Antimicrobial Activity

2020 ◽  
Vol 13 (12) ◽  
pp. 479
Author(s):  
Márcio Rodrigues ◽  
Ana Clara Lopes ◽  
Filipa Vaz ◽  
Melanie Filipe ◽  
Gilberto Alves ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Biological Activities ◽  
Scientific Information ◽  
Biological Properties ◽  
Fusarium Spp ◽  
Candida Spp ◽  
Pharmaceutical Industries

Thymus mastichina has the appearance of a semishrub and can be found in jungles and rocky lands of the Iberian Peninsula. This work aimed to review and gather available scientific information on the composition and biological properties of T. mastichina. The main constituents of T. mastichina essential oil are 1,8-cineole (or eucalyptol) and linalool, while the extracts are characterized by the presence of flavonoids, phenolic acids, and terpenes. The essential oil and extracts of T. mastichina have demonstrated a wide diversity of biological activities. They showed antibacterial activity against several bacteria such as Escherichia coli, Proteus mirabilis, Salmonella subsp., methicillin-resistant and methicillin-sensitive Staphylococcus aureus, Listeria monocytogenes EGD, Bacillus cereus, and Pseudomonas, among others, and antifungal activity against Candida spp. and Fusarium spp. Additionally, it has antioxidant activity, which has been evaluated through different methods. Furthermore, other activities have also been studied, such as anticancer, antiviral, insecticidal, repellent, anti-Alzheimer, and anti-inflammatory activity. In conclusion, considering the biological activities reported for the essential oil and extracts of T. mastichina, its potential as a preservative agent could be explored to be used in the food, cosmetic, or pharmaceutical industries.

scholarly journals GC-MS analysis and biological activities of Thymus vulgaris and Mentha arvensis essential oil

2019 ◽  
Vol 44 (3) ◽  
pp. 388-396
Author(s):  
Ashfaq Ahmad Khan ◽  
Muhammad Shoaib Amjad ◽  
Saboon
Keyword(s):  
Antioxidant Activity ◽  
Essential Oil ◽  
Biological Activities ◽  
Colorimetric Method ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Thymus Vulgaris ◽  
Dpph Assay ◽  
Mentha Arvensis ◽  
Ms Analysis

Abstract Background Essential oils are chemical products produced by odoriferous glands from a variety of plants. These essential oil have many health benefits i.e. antiseptic, anti-inflammatory and antimicrobial activities. So due to these medicinal properties present study was designed to analyze essential oil of Thymus vulgaris and Mentha arvensis for their chemical composition and biological activities. Materials and methods Essential oil from these plants were extracted by hydrodistillation method, and analyzed by GC-MS. To test the microbial activity of these oil disk diffusion method and micro wells method were used. For free radical scavenging DPPH assay was used. However total phenolic content was measured by colorimetric method. Results The GC-MS analysis of T. vulgaris oil showed the presence of 47 chemical compounds among which thymol, terpinene, p-cymene and carvacrol were major. However essential oil of M. arvensis showed the presence of 28 constituents, among which the Menthone, Menthol, Isomenthone, Eucalyptol, neo-Menthol, cis-Piperitone oxide, Linalool, Thymol, Limonene, and α-Phellandrene were major. Essential oil from both these plant tested for antimicrobial activity showed that the T. vulgaris oil was effective against seven bacterial strains and the essential oil of M. arvensis was effective against six bacterial strain. The antioxidant activity of both samples by DPPH assay which showed positive result. Conclusion As both species showed the presence of active components, positive microbial activities, and antioxidant activity so, research should be carried on for further biological activities of these oil for betterment of living beings.

scholarly journals Synthesis and biological activities of some indoline derivatives

2009 ◽  
Vol 74 (12) ◽  
pp. 1377-1387 ◽  
Author(s):  
Milind Rode ◽  
Sahebrao Rindhe ◽  
Bhausaheb Karale
Keyword(s):  
Antioxidant Activity ◽  
Aromatic Amines ◽  
Benzoyl Chloride ◽  
Stannous Chloride ◽  
Biological Activities ◽  
Gram Negative Bacteria ◽  
Good Activity ◽  
Dpph Assay ◽  
Gram Negative

The reaction of indoline with a substituted benzoyl chloride in the presence of K2CO3 in THF gave compound 4. Compound 4 was subjected to chlorosulphonation to obtain compound 5. Condensation of aromatic amines with compound 5 led to the synthesis of indoline derivatives 6(a-f). Similarly, 5-nitroindoline was treated with a substituted benzoyl chloride to obtain the nitro compound 9, which was reduced using stannous chloride and reacted further with aromatic sulphonyl chloride to obtain the indoline derivatives 11(a-e). These compounds were tested for antibacterial, anti-tuberculosis and antifungal activity. Some of them showed very good activity against some gram-positive and gram negative bacteria, fungal strains and also Mycobacterium tuberculosis. All of the synthesized compounds were subjected to antioxidant activity testing using the in vitro DPPH assay and most of them showed very good activity.

scholarly journals Heat-stable enterotoxins from Escherichia coli P16

1980 ◽  
Vol 28 (3) ◽  
pp. 1038-1040
Author(s):  
M N Burgess ◽  
N A Mullan ◽  
P M Newsome
Keyword(s):  
Escherichia Coli ◽  
Ion Exchange ◽  
Isoelectric Focusing ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Infant Mouse ◽  
Heat Stable

Escherichia coli P16 infant mouse active heat-stable enterotoxin may be fractionated into two distinct active moieties by ion-exchange chromatography, Sephadex G-25 chromatography, and isoelectric focusing.

scholarly journals Natural Phytochemicals and Biological Activities of Wild Grape (Ampelocissus martinii Planch.) Root Extract

2021 ◽  
Vol 33 (3) ◽  
pp. 545-550
Author(s):  
P. Siripipatthana ◽  
P. Srihanam ◽  
A. Sangdee
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Antibacterial Activities ◽  
Root Extract ◽  
Phytochemical Content ◽  
Gram Positive Bacteria ◽  
High Antioxidant Activity

A hydromethanolic root extract of Ampelocissus martinii Planch. (A. martinii) was analyzed by standard methods for its phytochemical content, antioxidant activity, α-amylase and α-glucosidase inhibitions and antibacterial activities. The root extract exhibited the highest content of saponins, followed by phenols, proanthocyanidin and flavonoids, respectively. It showed high antioxidant activity in FRAP and CUPRAC assays. The root extract and standard Trolox had similar antioxidant activities in the DPPH and ABTS assay. It also showed much higher α-amylase and α-glucosidase inhibitory activity compared to standard acarbose. Moreover, the root extract inhibited all tested Gram-positive bacteria with the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 6.25 mg/mL. These results indicate that A. martinii root can be pharmaceutically used as active ingredients to prevent bacterial infection and radical-related diseases especially diabetes.

scholarly journals Synthesis and Evaluation of Antioxidant Properties of 2-Substituted Quinazolin-4(3H)-ones

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6585
Author(s):  
Janez Mravljak ◽  
Lara Slavec ◽  
Martina Hrast ◽  
Matej Sova
Keyword(s):  
Antioxidant Activity ◽  
Phenyl Ring ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Hydroxyl Group ◽  
Hydroxyl Groups ◽  
Ortho Position ◽  
Dpph Assay ◽  
Metal Chelating ◽  
Methoxy Substituent

Quinazolinones represent an important scaffold in medicinal chemistry with diverse biological activities. Here, two series of 2-substituted quinazolin-4(3H)-ones were synthesized and evaluated for their antioxidant properties using three different methods, namely DPPH, ABTS and TEACCUPRAC, to obtain key information about the structure–antioxidant activity relationships of a diverse set of substituents at position 2 of the main quinazolinone scaffold. Regarding the antioxidant activity, ABTS and TEACCUPRAC assays were more sensitive and gave more reliable results than the DPPH assay. To obtain antioxidant activity of 2-phenylquinazolin-4(3H)-one, the presence of at least one hydroxyl group in addition to the methoxy substituent or the second hydroxyl on the phenyl ring in the ortho or para positions is required. An additional ethylene linker between quinazolinone ring and phenolic substituent, present in the second series (compounds 25a and 25b), leads to increased antioxidant activity. Furthermore, in addition to antioxidant activity, the derivatives with two hydroxyl groups in the ortho position on the phenyl ring exhibited metal-chelating properties. Our study represents a successful use of three different antioxidant activity evaluation methods to define 2-(2,3-dihydroxyphenyl)quinazolin-4(3H)-one 21e as a potent antioxidant with promising metal-chelating properties.

scholarly journals Separation and Identification of Highly Efficient Antioxidant Peptides from Eggshell Membrane

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 495
Author(s):  
Qian-Cheng Zhao ◽  
Jie-Yuan Zhao ◽  
Dong Uk Ahn ◽  
Yong-Guo Jin ◽  
Xi Huang
Keyword(s):  
Antioxidant Activity ◽  
Water Solubility ◽  
Biological Activities ◽  
Reducing Power ◽  
Thiobarbituric Acid ◽  
Exchange Chromatography ◽  
Eggshell Membrane ◽  
Degree Of Hydrolysis ◽  
Hydrolysis Time ◽  
Antioxidant Agents

The enzymatic hydrolysates (EHs) of the eggshell membrane (ESM) were obtained after incubating eggshell membrane in solutions prepared with Na2SO3 and alkaline protease combinations. The effects of enzyme species, enzyme dosage, Na2SO3 concentration, and hydrolysis time on the antioxidant activity of the ESM-EH were determined. Also, the correlation between the degree of hydrolysis (DH) and the antioxidant activity of ESM-EH was analyzed. The DH of ESM-EH showed a highly positive correlation with the reducing power (R2 = 0.857) and total antioxidant activity (TAA) (R2 = 0.876) and performed negative correlation with the Fe2+-chelating ability (R2 = −0.529). The molecular weight distribution of the ESM-EH was determined by MALDI-TOF/MS. Cation exchange chromatography (Sephadex C-25) was used to isolate the ESM-EH and then the enzymatic hydrolysis fragment (EHF) was obtained. Among the five isolated fragments (F1~F5), fragment 3 (F3), which was composed of 28 polypeptides, showed the highest ability to quench ABTS• (2,2-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid) (90.44%) and also displayed stronger TBARS (thiobarbituric acid– reactive substances) (58.17%) and TAA (303.82 µg /mL) than the ESM-EH. Further analysis of the 28 peptides in F3 identified using LC-MS/MS indicated that five peptides (ESYHLPR, NVIDPPIYAR, MFAEWQPR, LLFAMTKPK, MLKMLPFK) showed high water-solubility, biological activities, and antioxidant characteristics. Finally, the TAA of the synthetic peptide was verified, the synthetic peptides ESYHLPR and MFAEWQPR performed the best activity and have high potentials to be used as antioxidant agents in functional foods, pharmaceuticals, or cosmetics.

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