Natural Phytochemicals and Biological Activities of Wild Grape
(Ampelocissus martinii Planch.) Root Extract

A hydromethanolic root extract of Ampelocissus martinii Planch. (A. martinii) was analyzed by standard methods for its phytochemical content, antioxidant activity, α-amylase and α-glucosidase inhibitions and antibacterial activities. The root extract exhibited the highest content of saponins, followed by phenols, proanthocyanidin and flavonoids, respectively. It showed high antioxidant activity in FRAP and CUPRAC assays. The root extract and standard Trolox had similar antioxidant activities in the DPPH and ABTS assay. It also showed much higher α-amylase and α-glucosidase inhibitory activity compared to standard acarbose. Moreover, the root extract inhibited all tested Gram-positive bacteria with the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 6.25 mg/mL. These results indicate that A. martinii root can be pharmaceutically used as active ingredients to prevent bacterial infection and radical-related diseases especially diabetes.

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Antimicrobial and Antioxidant Activities of Flavonoids Extracted from Pistacia lentiscus L., Leaves

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i1-s.3895 ◽

2020 ◽

Vol 10 (1-s) ◽

pp. 83-89

Author(s):

Sabrina Bakli ◽

Harzallah Daoud ◽

Zerroug Amina ◽

Sadrati Nouari ◽

Bouguerra Asma ◽

...

Keyword(s):

Antioxidant Activity ◽

Inhibitory Concentration ◽

Solid Medium ◽

Minimum Bactericidal Concentration ◽

Antioxidant Activities ◽

Biological Activities ◽

Reducing Power ◽

Dilution Method ◽

Pistacia Lentiscus ◽

The Subject

Increasingly, the flavonoids becoming the subject of biomedical researches, and many groups have isolated and identified the structures of flavonoids possessing important biological activities. This assess investigates the antimicrobial fight and the antioxidant strengths of flavonoids extracted from Pistacia lentiscus L. leaves. The extract was first analyzed for its phenolic content, then the flavonoids were tested for their antimicrobial capacities by the method of diffusion against fourteen bacteria, two fungi and one yeast. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of flavonoids were determined using the dilution method on solid medium, however, the antioxidant activity was evaluated through the ability of the extract to scavenge DPPH (1,1-diphenyl-2-picrylhydrazyl) radicals and the reducing power essay. The results indicate that flavonoids extract contain other phenolics groups. On the other side, the Antibacterial tests exhibited different activities depending on the strains. Unlike, the MIC and the MBC showed that the flavonoids extract showed a best activity against Candida albicans and Vibrio cholerae. Concerning antioxidant activity, the results indicate a moderate capacity of the flavonoid extract. These preliminary results may justify the use of this plant in the treatment of some diseases and may be exploited for therapeutic purposes. Keywords: Antimicrobial, Antioxidant, Pistacia lentiscus, flavonoids, MIC, MBC.

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Antibacterial and Antioxidant Activities of Slender Amaranth Weed

Planta Daninha ◽

10.1590/s0100-83582020380100006 ◽

2020 ◽

Vol 38 ◽

Cited By ~ 1

Author(s):

M. AKBAR ◽

I.N. SHERAZI ◽

M.S. IQBAL ◽

T. KHALIL ◽

H.M. WAQAS

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Ethyl Acetate ◽

Pseudomonas Syringae ◽

Antioxidant Activities ◽

Biological Activities ◽

Radical Scavenging Activity ◽

Bacterial Species ◽

Radical Scavenging ◽

Root Extract

ABSTRACT: In the present study, antibacterial and antioxidant [1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity] activities of a weed, slender amaranth (Amaranthus viridis L.) were investigated. Extracts of different plant parts were prepared in n-hexane, chloroform and ethyl acetate. Antibacterial activity was measured by using 100 mg mL-1 concentration extracts against 4 deadly phytopathogenic bacterial species viz. Pseudomonas syringae Van Hall, Ralstonia solanacearum Smith, Erwinia carotovora (Jones), Holland and Xanthomonas axonopodis Hasse. In antioxidants assays, 10, 20 and 30 mg mL-1 extracts were used keeping DPPH as control. In these bioassays, ethyl acetate fraction of A. viridis leaf exhibited the best antibacterial and antioxidants activity. Ethyl acetate leaf fraction showed the highest inhibition zone diameter (IZD) where it caused 21 mm IZD against P. syringae and 19 mm IZD against E. carotovora. This extract also showed 22, 52 and 84% antioxidant activity at 10, 20 and 30 mg mL-1 concentrations, respectively. Previously there is no report available that describes antibacterial activity of root extract of A. viridis against P. syringae. Moreover, antioxidant activity of stem and root extracts in n-hexane, chloroform and ethyl acetate was investigated first time in the world. It was concluded that the biological activities observed during the present investigation may be due to the presence of bioactive constituents that can be harnessed as natural antibacterials and antioxidants.

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Antimicrobial Activity and Nutraceutical Potential of Tuscan Bee-Pollens on Oxidative and Endoplasmic Reticulum Stress in Different Cell-Based Models

Proceedings ◽

10.3390/foods_2020-07749 ◽

2020 ◽

Vol 70 (1) ◽

pp. 108

Author(s):

Morena Gabriele ◽

Stefania Frassinetti ◽

Laura Pucci

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Endoplasmic Reticulum ◽

Endoplasmic Reticulum Stress ◽

Er Stress ◽

Biological Activities ◽

Bee Pollen ◽

Phytochemical Content ◽

Gram Positive Bacteria ◽

Species Production

Bee-pollen is an apiary product of great interest owing to its high nutritional and therapeutic properties. This study aimed to assess the cellular antioxidant activity and the antihemolytic effects of Castanea, Rubus, and Cistus bee-pollens on oxidized human erythrocytes. In addition, the antimicrobial potential of each sample was tested on three Gram-negative and two Gram-positive bacteria. Finally, the effect of Castanea bee-pollen, showing better phytochemical content, was analyzed on human microvascular endothelial cells (HMEC-1) exposed to thapsigargin, used to induce endoplasmic reticulum stress (ER-stress). Our results showed good biological activities of all bee-pollen samples, which, under oxidative conditions, significantly improved the erythrocytes’ antioxidant activity and limited cell lyses. Moreover, all samples exerted antimicrobial activity with different selectivity among the tested microorganisms, with minimal inhibitory concentration values ranging from 5 to 10 mg/mL. Finally, thapsigargin treatment increased intracellular ROS (reactive oxygen species) production and up-regulated the expression of factors involved in the ER-stress and inflammatory pathways. Conversely, Castanea bee-pollen was effective in reducing gene overexpression, as well as the oxidation process arising from thapsigargin treatment, with a maximum protective effect at 10 µg/mL. In conclusion, bee-pollens, mainly Castanea species, represent good natural antibacterial and potential nutraceutical products useful in the prevention of free radical and ER-stress associated diseases.

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Improvement of cytoprotective and antioxidant activity of Rosa canina L. and Salix alba L. by controlled differential sieving process against H2O2-induced oxidative stress in mouse primary splenocytes

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000506 ◽

2017 ◽

Vol 87 (3-4) ◽

pp. 191-200 ◽

Cited By ~ 1

Author(s):

Nidhal Soualeh ◽

Aliçia Stiévenard ◽

Elie Baudelaire ◽

Rachid Soulimani ◽

Jaouad Bouayed

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Biological Activities ◽

Good Alternative ◽

Activity Levels ◽

Salix Alba ◽

Rosa Canina ◽

Free Process ◽

Plant Powders ◽

Sieving Process

Abstract. In this study, cytoprotective and antioxidant activities of Rosa canina (RC) and Salix alba (SA), medicinal plants, were studied on mouse primary splenocytes by comparing Controlled Differential Sieving process (CDSp), which is a novel green solvent-free process, versus a conventional technique, employing hydroethanolic extraction (HEE). Thus, preventive antioxidant activity of three plant powders of homogeneous particle sizes, 50–100 µm, 100–180 µm and 180–315 µm, dissolved directly in the cellular buffer, were compared to those of hydroethanolic (HE) extract, at 2 concentrations (250 and 500 µg/mL) in H2O2-treated spleen cells. Overall, compared to HE extract, the superfine powders, i. e., fractions < 180 µm, at the lowest concentration, resulted in greater reactive oxygen species (ROS) elimination, increased glutathione peroxidase (GPx) activity and lower malondialdehyde (MDA) production. Better antioxidant and preventive effects in pre-treated cells were found with the superfine powders for SA (i. e., 50–100 µm and 100–180 µm, both p < 0.001), and with the intermediate powder for RC (i. e., 100–180 µm, p < 0.05) versus HE extract. The activity levels of catalase (CAT) and superoxide dismutase (SOD) in pretreated splenocytes exposed to H2O2, albeit reduced, were near to those in unexposed cells, suggesting that pretreatment with the fine powders has relatively restored the normal levels of antioxidant-related enzymes. These findings supported that CDSp improved the biological activities of plants, avoiding the use of organic solvents and thus it could be a good alternative to conventional extraction techniques.

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Optimising Conditions for Encapsulation of Salacia chinensis Root Extract enriched with Phenolic Compounds

Current Nutraceuticals ◽

10.2174/2665978602666210805094012 ◽

2021 ◽

Vol 02 ◽

Author(s):

Thanh V. Ngo ◽

Christopher J. Scarlett ◽

Michael C. Bowyer ◽

Rebecca McCullum ◽

Quan V. Vuong

Keyword(s):

Antioxidant Activity ◽

Spray Drying ◽

Feed Rate ◽

Freeze Drying ◽

Antioxidant Activities ◽

Inlet Temperature ◽

Root Extract ◽

Optimal Conditions ◽

Dried Extract ◽

Drying Conditions

Background: S. chinensis extract contains bioactive compounds, which exhibit high antioxidant activities. However, for commercial uses, it is necessary to encapsulate the extract to protect it from degradation. Objective: This study aimed to optimise spray-drying conditions and then compare with freeze-drying to identify the most suitable conditions for encapsulation of Salacia chinensis L. root extract. Method: Three factors of spray-drying encapsulation, including maltodextrin concentration, inlet temperature and feed rate, have been tested for the impacts on the physical and phytochemical properties of S. chinensis root extract. Based on the optimal conditions, the spray-drying was then compared with freeze-drying. Results: The results showed that maltodextrin concentration, inlet temperature and feed rate had significant impacts on recovery yield, phenolics, mangiferin and antioxidant activity of the spray-dried extract. The optimal spray-drying encapsulation conditions were maltodextrin concentration of 20 %, inlet temperature of 130ºC and feed rate of 9 mL/min. Under these optimal conditions, the encapsulated extract had comparable solubility, total phenolics, mangiferin, and antioxidant activity, lower bulk density, moisture content, and water activity as compared to encapsulated extract made using the freeze-drying technique. These optimal spray-drying conditions are recommended to encapsulate the extract of S. chinensis root. Conclusion: Spray-drying was found to be more effective for encapsulation of S. chinensis root extract than freeze-drying. Therefore, spray-drying is recommended for further applications.

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ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF THE ESSENTIAL OIL OF CUMINUM CYMINUM SEEDS

AGROFOR ◽

10.7251/agreng2101079b ◽

2021 ◽

Vol 6 (1) ◽

Author(s):

Amina BESSEDIK

Keyword(s):

Antioxidant Activity ◽

Essential Oil ◽

Antioxidant Activities ◽

Antibacterial Activities ◽

Biological Properties ◽

Bioactive Molecules ◽

Natural Food ◽

Cuminum Cyminum ◽

Research Fields ◽

Methanolic Extracts

Cuminum cyminum (in arabic: Kemoun) is an important source of therapeutic, cosmetic, bio-food and technological agents. Ancient civilizations used this plant for therapeutic purposes. Extracts from this plant are nowadays strongly used in the industrial and research fields, particularly for the medicinal, pharmacological and cosmetological purposes. Bioactive molecules extracted from Cuminum cyminum may express biocidal activities and prove to be good candidates for new antioxidants. The objective of the present work is to evaluate the biological properties of this plant, including antibacterial and antioxidant effects. The seeds of cumin are harvested at the wilaya of Biskra, and stored in a dry place until their use. They were identified at Hassiba Benbouali University of Chlef. Essential oil is obtained by hydro-distillation using a Clivenger type device (AFNOR, 2000). The obtained sample is stored in sealed bottles at low temperature (4°C) and away from light. The antibacterial effect is assessed by the disc method. The minimum inhibitory concentration (MIC) is determined by standard methods. The antioxidant activity is evaluated by the DPPH free radical trapping method of the methanolic extracts. The essential oil of C. cyminum shows a better activity against Gram+ strains compared to Gram- strains. The determination of MICs leads to the conclusion that its activity can be triggered at a very low concentration. The reaction with DPPH gives an interesting IC50. This testifies to the ability of this essential oil to reduce free radicals. Thanks to the antibacterial activities that we have highlighted, cumin can be considered as a palliative that could replace certain antibiotics. Moreover, its antioxidant activity allows us to use it as a natural food additive.

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In-Vitro Antioxidant, Antibacterial and Anticancer properties of Leaf Extract Annona Muricata

International Journal of Advanced Science and Engineering ◽

10.29294/ijase.8.1.2021.2119-2126 ◽

2021 ◽

Vol 8 (1) ◽

Keyword(s):

Antioxidant Activity ◽

Anticancer Activity ◽

Antioxidant Activities ◽

Extraction Procedure ◽

Radical Scavenging ◽

Antibacterial Activities ◽

Diffusion Method ◽

Annona Muricata ◽

Anticancer Properties

Annona muricata is one of the important herbal plant that are widely used to treat antidiabetic, anti-inflammatory, insecticidal, antimalarial, anticancer, antibacterial and antioxidant activities. In the present study, extraction procedure was carried out in few step processes method. The extract contains high percentage of steroid, alkaloid, flavonoid, phenolic and saponin. The extract was used to study the antioxidant activity; antibacterial activities and anticancer activity by standard methods. The antioxidant activity was studied by using radical scavenging DPPH, FRAP and H2O2 method and disc diffusion method. The results of antibacterial activity of the following bacteria’s such as Pseudomonas, Staphylococcus, Bacillus, E.coli shows the maximum zone of inhibition against Staphylococcus aureus which is around 1.7 cm. The anticancer activity was carried out by MTT assay using Hep-G2 as cell line and results are reported in the paper.

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Synthesis, Antimicrobial and Antioxidant Activities of 2-Isoxazoline Derivatives

Molecules ◽

10.3390/molecules25184271 ◽

2020 ◽

Vol 25 (18) ◽

pp. 4271

Author(s):

Asma Alshamari ◽

Mahmoud Al-Qudah ◽

Fedaa Hamadeh ◽

Lo’ay Al-Momani ◽

Sultan Abu-Orabi

Keyword(s):

Antioxidant Activity ◽

Sulfonic Acid ◽

Antioxidant Activities ◽

Bacterial Species ◽

Antibacterial Activities ◽

Ms Analysis ◽

Diammonium Salt ◽

New Compounds ◽

Derivatives Of

A series of derivatives of trans-3-(2,4,6-trimethoxyphenyl)4,5-dihydroisoxazolo-4,5-bis[carbonyl-(4′phenyl)thiosemicarbazide (9) and of trans-3-(2,4,6-trimethoxyphenyl)-4,5-dihydro isoxazolo-4,5-bis(aroylcarbohydrazide) (10a–c) were synthesized from trans-3-(2,4,6-trimethoxyphenyl)-4,5-dihydro-4,5-bis(hydrazenocarbonyl)isoxazole (8). The structures of the compounds were elucidated by both elemental and spectral (IR, NMR, and MS) analysis. Compound 9 shows activity against some bacterial species. No antibacterial activities were observed for compounds 10a–c. The antioxidant activity of the new compounds has been screened. Compound 9 showed higher antioxidant activity using the DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2’-azino–bis(3-ethylbenzoline-6-sulfonic acid) diammonium salt methods.

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Ferrocene Derivatives Carrying Urea, Thiourea, and Sulfonamide Moieties: Synthesis and Evaluation of Antibacterial and Antifungal Activities

Journal of Chemistry ◽

10.1155/2013/149693 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 8

Author(s):

Serkan Yavuz ◽

Hilal Yıldırım

Keyword(s):

Inhibitory Concentration ◽

Antibacterial Activities ◽

Klebsiella Pneumonia ◽

Gram Negative Bacteria ◽

Ferrocene Derivatives ◽

Antifungal Activities ◽

Gram Positive Bacteria ◽

Antibacterial And Antifungal Activities ◽

Elemental Analyses ◽

Antibacterial And Antifungal

In the present study, some novel ferrocene derivatives carrying urea, thiourea, and sulfonamide groups were synthesized, and all compounds were characterized by spectral and elemental analyses. These compounds were screened for their antibacterial activities and also their minimum inhibitory concentration (MIC) against Gram-positive bacteria (Staphylococcus aureusandBacillus subtilis) and Gram-negative bacteria (Klebsiella pneumoniaandEscherichia coli) and antifungal activities againstSaccharomyces cerevisiaeandCandida albicans. Amongst the tested compounds,4b,4c,5b, and6bdisplayed excellent antimicrobial activity.

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Prenylated Flavonoids as Antioxidant and Melanin Inhibitors From Stingless Bee (Wallacetrigona incisa) Propolis

Natural Product Communications ◽

10.1177/1934578x20911272 ◽

2020 ◽

Vol 15 (3) ◽

pp. 1934578X2091127

Author(s):

Enos Tangke Arung ◽

Whicliffe Fiernaleonardo Pasedan ◽

Nataniel Tandirogang ◽

Ahmed E. Allam ◽

Yhiya Amen ◽

...

Keyword(s):

Sulfonic Acid ◽

Tropical Rainforest ◽

Inhibitory Concentration ◽

Methanol Extract ◽

Antioxidant Activities ◽

Biological Activities ◽

Stingless Bee ◽

B16 Melanoma ◽

Prenylated Flavonoids ◽

Tyrosinase Enzyme

Propolis from 4 stingless bees ( Homotrigona apicalis, Wallacetrigona incisa, Tetragonula fuscobalteata, and Tetragonula fuscibasis) was investigated in the search for medicinal and cosmetic materials from tropical rainforest resources. Methanol extracts of the propolis were screened using antioxidant and antimelanogenesis assays (tyrosinase enzyme activity and melanin inhibitor in B16 melanoma). The extract of H. apicalis showed the strongest antioxidant activity, both in the 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays, with half-maximal inhibitory concentration values of 0.72 ± 0.01 (mg/mL) and 0.26 ± 0.00 (mg/mL), respectively. The H. apicalis extract also displayed the strongest inhibition of tyrosinase (53% at 100 µg/mL). In the B16 melanoma cell assay, the W. incisa extract showed the strongest inhibition of melanin (21%) and was less cytotoxic. The W. incisa extract was fractioned to isolate the compounds with biological activities. Two prenylated flavonoids were obtained, named broussoflavonol F and glyasperin A. Both showed potent antioxidant activities, as well as inhibiting melanin in B16 melanoma, but not tyrosinase activity. These results indicated the potential of methanol extract of W. incisa to be developed for cosmetic material, but further experiments are needed to verify the function.

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