scholarly journals Dynamics of proinflammatory cytokine serum levels in patients with acute inflammatory diseases of pelvic organs in the early stages of conservative treatment

2019 ◽  
Vol 9 (3-4) ◽  
pp. 612-616
Author(s):  
N. A. Burova ◽  
P. A. Soltys ◽  
N. A. Zharkin ◽  
M. S. Selikhova ◽  
N. I. Sviridova ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Proinflammatory Cytokines ◽  
Inflammatory Diseases ◽  
Low Frequency ◽  
Serum Levels ◽  
Reproductive Age ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
Pelvic Organs ◽  
Frequency Laser

Dynamics of serum proinflammatory cytokines such as interleukins (IL)-1, -2 and -6, tumor necrosis factor alpha (TNFα) was examined in patients of reproductive age suffering from acute inflammatory diseases of the pelvic organs. Our study was carried out with female patients at hospital admission prior to therapy applied with conventional methods of treatment and improved treatment by using vaginal low-frequency laser in a constant continuous magnetic field. The data obtained were compared with serum cytokine level from 20 healthy female volunteers of reproductive age consulted on better contraception methods. It was found that cytokine profile of in patients with acute inflammatory processes in pelvic organs was characterized by a high level of proinflammatory cytokines. It was shown that patients receiving conventional treatment contained decreased level of serum IL-1, IL-2, IL-6 and TNFα displaying slight dynamics, which did not reach it in control group. This may contribute to ongoing inflammatory process, despite the positive clinical dynamics. In turn, imbalance of immune responses leads to a persistently impaired fertility in women and need to perform subsequent comprehensive rehabilitation measures. Moreover, patients applied with intravaginal low-frequency laser radiation in a constant magnetic field were found to contain serum TNFα < 100 pg/ml observed in 59.7% of cases, IL-6 level was lower than 20 pg/ml (prevalent in control group) found in 54.2% of cases. Serum IL-2 level was decreased by 3.5-fold compared to baseline, whereas for IL-1β it was higher than 100 pg/ml in as few as 23.6% patients. Such temporal pattern of inflammatory markers with rapid significant decrease of serum proinflammatory cytokines in patients with preformed pathogenic factors can reduce probability of connective tissue formation and activate their own repair as well as regenerative events. The results obtained allow to wider use combined physical interventional factors for therapy of patients with acute inflammatory diseases of the pelvic organs.

New approaches in complex therapy of inflammatory diseases of the pelvic organs in women of reproductive age

2017 ◽  
pp. 41-44
Author(s):  
O.V. Grischenko ◽  
◽  
T.A. Kozub ◽  
Keyword(s):  
Inflammatory Diseases ◽  
Reproductive Age ◽  
Control Group ◽  
Study Group ◽  
Complex Treatment ◽  
Women Of Reproductive Age ◽  
Urine Ph ◽  
Pelvic Organs ◽  
Standard Scheme ◽  
Group I

The objective: to study the efficacy of complex treatment of inflammatory diseases of the pelvic organs (vaginitis), occurring in conjunction with urinary tract infection (cystitis), with a combined medication of plant origin Tutukon produced by Miguel and Garriga, S. A. ("Grand medical Group AG", Spain/Switzerland) in women of reproductive age. Patients and methods. Were examined 60 women of reproductive age with the presence of vaginitis and associated cystitis. All the patients were divided into two clinical groups. In group I (control; n=30) treatment was carried out according to the standard scheme with the use of antimicrobial drugs, antispasmodics, local antiseptics for the sanitation of the vagina. In group II (study group; n=30) treatment was carried out according to the standard scheme with the inclusion of the drug Tutukon. All the patients were examined at 3-rd, 7-th, 10-th day of treatment and 30 days after discontinuation of therapy. Results. During the research was given the analysis of the dynamics of clinical and laboratory parameters in women of both groups before and after treatment. In patients of study group, the standard therapy which included Tutukon, the treatment was more effective than in patients of control group: a rapid onset of clinical effect, reducing the duration of the disease, a complete clinical response in 90% of cases versus 63%, alkalization of urine - pH 6.5±0.10, the absence of recurrence within 30 days after the end of therapy. Conclusion. Tutukon medicine is recommended for complex treatment of vaginitis, occurring in combination with cystitis in women of reproductive age. Key words: vaginitis, cystitis, dysuria, urine pH, Tutukon.

The ways to ameliorate the efficiency of treatment for women with pelvic inflammatory disease

2020 ◽  
pp. 54-58
Author(s):  
I.V. Lakhno ◽  
◽  
A.E. Tkachov ◽  
O.V. Grischenko ◽  
◽  
...  
Keyword(s):  
Abdominal Pain ◽  
Vaginal Discharge ◽  
Inflammatory Diseases ◽  
Lower Abdominal Pain ◽  
Resistance Index ◽  
Reproductive Age ◽  
Infusion Therapy ◽  
Control Group ◽  
Pelvic Organs ◽  
Abnormal Vaginal Discharge

The inflammatory diseases of the pelvic organs are known to be one of the important problems of modern gynecology, which is due to the high frequency of chronicity of the process and a large number of complications. The article provides a theoretical and practical substantiation of the use of the drugs Rheosorbilact and Grandazol to increase the efficacy of empirical therapy in patients with acute salpingo-oophoritis. The objective: study of the effectiveness of the use of hyperosmolar combined solution of sorbitol with a balanced content of electrolytes for low-volume infusion therapy (Rheosorbilact) and a solution of levofloxacin 2.5 mg and ornidazole 5 mg (Grandazole) in the treatment of women with acute PID. Materials and methods. Totally 98 women of reproductive age were examined and divided into three clinical groups. The I (control) group included 30 healthy women. In group II (comparison), 33 patients with acute bilateral adnexitis were included, who were prescribed traditional treatment. In group III (main) group, there were 35 women who were additionally prescribed Rheosorbilact and Grandazole was used as an antibacterial agent. Results. A survey of women with acute bilateral adnexitis found that among all clinical characteristics the most common were: lower abdominal pain (89.7%), abnormal vaginal discharge (88.2%), and fever (83.8%). The variables of the level of C-reactive protein, the number of leukocytes, and the value of the resistance index in the ovarian arteries were elevated in the study population before treatment, respectively: 52.4±8.3 mg/l, 11.8±2.2×109/l, 1.4±0.2, which differed from the control values (p<0.05). The study of the pseudo-R2 Nagelkerke confirmed the relationship between increased resistance index and lower abdominal pain, abnormal vaginal discharge and fever with high sensitivity (88,2%) and insufficient specificity (60.4%). Thus, in women with acute salpingo-oophoritis, the presence of inflammatory symptoms (even in the absence of Doppler data) is an indication for the use of infusions of vasotrophic drugs with the hemorheological action.The priority effect of this method of treatment was the optimization of hemodynamics in the large vessels of the small pelvis, which provided the effective delivery and accumulation of antibacterial drugs in the inflammation focus. Conclusion. The specified combination of drugs Rheosorbilact and Grandazole has demonstrated the maximum clinical effect, which justifies the possibility of widespread use of this method of therapy. Keywords: inflammatory diseases of the pelvic organs, Doppler ultrasound, treatment, Rheosorbilact, Grandazole.

scholarly journals Severity of inflammatory syndrome and endothelial dysfunction in steatosis and liver fibrosis

2021 ◽  
Vol 38 (4) ◽  
pp. 54-61
Author(s):  
I. A. Bulatova ◽  
A. M. Miftakhova ◽  
I. L. Gulyaeva
Keyword(s):  
Endothelial Dysfunction ◽  
Liver Fibrosis ◽  
Proinflammatory Cytokines ◽  
Functional Activity ◽  
Liver Steatosis ◽  
Serum Levels ◽  
Study Groups ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
Inflammatory Syndrome

Objective. To evaluate the severity of the inflammatory syndrome by the serum concentration of proinflammatory cytokines of tumor necrosis factor alpha (TNF-) and interleukin-6, endothelial dysfunction syndrome (ED) by the level of vasculoendothelial growth factor (VEGF) and the functional activity of Willebrand factor (WF) in the blood of patients with nonalcoholic liver steatosis (NALS) and liver fibrosis (LF) of viral genesis. Materials and methods. 52 patients with NALS and 27 patients with LF of viral etiology (hepatitis C) were examined. The control group included 20 practically healthy individuals. The concentrations of TNF-, IL-6 and VEGF were determined in the blood by enzyme immunoassay. The functional activity of WF was measured by the level of aggregation with the inducer ristocetin using laser aggregometer. Results. According to the results of ELISA, an increase in serum levels of proinflammatory cytokines TNF- and IL-6 was registered in patients of both study groups in comparison with the control, being more pronounced in patients with viral LF. Hyperproduction of VEGF was observed in both groups of patients, and the concentration of this marker was significantly higher in viral LF than in patients with NALS (p = 0.002). The functional activity of WF in patients with NALS and in the group with LF also significantly exceeded the control values, but there were no significant differences between the nosological forms (p = 0.675). Conclusions. The course of NALS and viral LF is characterized by the development of an inflammatory syndrome and ED, associated with an increase in the production of proinflammatory cytokines TNF- and IL-6 and hyperproduction of VEGF, more pronounced in LF. The functional activity of WF also increases in both nosological forms, but without significant differences.

scholarly journals The Effect of 12 Hz Extremely Low-Frequency Electromagnetic Fields on Visual Memory of Male Macaque Monkeys

2021 ◽  
Author(s):  
Masoomeh Kazemi ◽  
◽  
Hamed Aliyari ◽  
Elaheh Tekieh ◽  
Hassan Tavacoli ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Visual Memory ◽  
Rhesus Macaques ◽  
Low Frequency ◽  
Serum Levels ◽  
Control Group ◽  
Behavioral Tests ◽  
Extremely Low Frequency ◽  
Before And After ◽  
Male Rhesus

Introduction: Today, humans leave in a world surrounded by electromagnetic fields. Numerous studies have been carried out to discover the biological, physiological, and behavioral effects of electromagnetic fields on humans and animals. Given the biological similarities between monkeys and humans, the goal of the present research was to examine Visual Memory (VM), hormonal, genomic, and anatomic changes, and changes of the amygdala function in the male rhesus macaques who exposed to extremely low-frequency magnetic fields (ELF/MF). Materials and methods: Four male Rhesus Macaques (Macaca mulatta) were used. For the behavioral tests the animals were needed to be fasting for 17 hours, and for the behavioral tests such as visual memory, cooperation of the animal was necessary. Using the radiation protocol, two of the monkeys were exposed to 12-Hz electromagnetic fields with a magnitude of 0.7 microtesla (electromagnetic radiation) four hours a day for a month. Before and after the exposure, a visual memory test was conducted using a coated device (visible reward) on a movable stand. Ten mL of blood was obtained from the femoral artery of the monkeys and half of it was used to examine cortisol serum levels using MyBioSource kits (made in the USA). The other half of blood samples were used to extract lymphocytes for assaying expressions of Glucocorticoid Receptor (GR) genes before and after radiation using the PCR method. Anatomic studies of the amygdala were carried out based on pre- and post-radiation Magnetic Resonance Imaging (MRI). Findings: Research results indicated that visual memory in male primates increased significantly at the 12Hz frequency. Hormonal analysis at the 12Hz frequency showed a decrease in cortisol serum levels. However, visual memory and serum cortisol levels did not change considerably in male primates in the control group. There was no considerable amygdala volumetric difference at the 12 Hz frequency. The expression of the GR genes decreased at 12Hz compared to the control group. Conclusion: In short, these results indicated that ELF may have a beneficial value for memory enhancement as indicated by the fact that exposure to the 12 HZ ELF can enhance visual memory. This may be due to a decrease in plasma cortisol, and/or expression of GR genes. Moreover, direct involvement of the amygdala in this regard cannot be recommended.

scholarly journals Temperature and magnetic field effects on the activity of protocerebral neurosecretory neurons and corpora allata in Cerambyx cerdo L. larvae

2005 ◽  
Vol 57 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Vera Nenadovic ◽  
Marija Mrdakovic ◽  
Jelica Lazarevic ◽  
D. Mircic ◽  
Dajana Todorovic ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Constant Temperature ◽  
Low Frequency ◽  
Corpora Allata ◽  
Control Group ◽  
Magnetic Field Effects ◽  
Neurosecretory Neurons ◽  
Cerambyx Cerdo ◽  
Frequency Magnetic Field ◽  
A1 Neurons

The effects of constant temperature and an extremely low-frequency magnetic field (ELFMF, 50 Hz and average induction of 20 mT) on the activity of medial protocerebral neurosecretory neurons (A1 and A2) and corpora allata were investigated in Cerambyx cerdo L. larvae after 30 days of exposure. Both constant temperature of 23?C and the ELFMF led to decrease in activity of A1 and A2 neurosecretory neurons and increase in activity of corpora allata compared to the control group (larvae from natural conditions). The changes are more pronounced in A2 than A1 neurons.

The influence of low-frequency laser emission on phyto- and zooplankton productivity properties

2020 ◽  
Vol 12 (2) ◽  
pp. 196-201
Author(s):  
Lidiia Khuda ◽  
Larysa Cheban ◽  
Oleksii Khudyi
Keyword(s):  
Population Density ◽  
Catalase Activity ◽  
Laser Emission ◽  
Low Frequency ◽  
Positive Influence ◽  
The Other ◽  
Control Group ◽  
Microalgae Biomass ◽  
Frequency Laser ◽  
Positive Effect

We studied the possibility of using low frequency laser emission to improve production properties of feed hydrobionts, in particular microalgae Desmodesmus armatus and Daphnia magna, which are highly applied in aquaculture. It was shown that low-frequency laser emission with the wavelength 650 nm has a positive influence on production properties of both phyto- and zooplankton. In 24 hours the biomass of irradiated D. armatus was 25% larger than non-irradiated one. Meanwhile, application λ 420 and 530 nm did not show credible accumulation of microalgae biomass. If it comes to daphnia, positive dynamic in population density enlarging was observed only since 5-7th day of cultivation, depending on emission exposition. Differences in low-frequency laser emission with λ 420 and 530 efficacy on D. magna production properties were observed while using different exposition. Thus, maximal culture density was observed when λ 420 nm for 90 s and λ 650 nm for 60 s were applied. Longer irradiation with red laser did not have positive effect. It was established that in daphnia tissues, which were influenced by λ 420 nm laser emission, TBA-active products were accumulating more. Meanwhile, for daphnias, which were irradiated with red laser, TBA amount was not different from the control group one. Catalase activity increased while applying λ 650 nm laser emission. On the other hand, λ 420 nm did not cause credible increasing of catalase activity in comparison with control group. Taking received results about photobiological effects of low-frequency laser emission on D. armatus and D. magna into consideration, both biomasses enlargement during co-cultivation can be obtained if wavelength 650 nm is used.

189 THE EFFECT OF VITAMIN D SUPPLEMENTATION ON ANTI-MULLERIAN HORMONE LEVELS IN REPRODUCTIVE-AGE WOMEN

2015 ◽  
Vol 27 (1) ◽  
pp. 185 ◽  
Author(s):  
M. Taheri ◽  
M. Modarres ◽  
A. Abdollahi
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Intervention Group ◽  
Serum Levels ◽  
Vitamin D Supplementation ◽  
T Test ◽  
Reproductive Age ◽  
Control Group ◽  
Post Intervention ◽  
Reproductive Age Women

Vitamin D deficiency has been correlated with the infertility and lower clinical pregnancy following IVF. Anti-Mullerian hormone (AMH) plays a key role during follicle development; it has been recognised as a predictor of regular ovulation and probably IVF success. Considering the critical need for experimental human study to investigate the impact of vitamin D supplementation on ovulatory function, the aim of this study was to demonstrate the effectiveness of the vitamin D supplementation on AMH serum levels among reproductive-age women with vitamin D deficiency. 195 reproductive women (18–35 year-old) with confirmed vitamin D deficiency [serum 25(OH)D <75 nmol L–1] and without diagnosed polycystic ovary syndrome (PCOS) were enrolled to this controlled clinical trial. Participants were randomly assigned to a control group (n = 96) or an intervention group (n = 99). Women in the intervention group used 2000 IU day–1 vitamin D drops for 15 weeks. 19 participants were missed during the follow-up; finally the numbers of women in the intervention and control groups were 91 and 85, respectively. At the beginning of the study and after the intervention, 25-hydroxyvitamin D and AMH serum levels were quantified using enzyme immunoassay (EIA; Immunodiagnostic Systems, Boldon, UK) and ELISA (Beckman-Coulter Inc., Fullerton, CA, USA) methods respectively. The post-intervention AMH measurement was performed after 2–5 weeks in the same day-of-cycle on which basal AMH measurement was done. Paired t-test, independent t-test, and Pearson correlation were used as appropriate and a P-value of less than 0.05 was considered significant. Significantly low AMH levels were seen in the vitamin D deficient women of this study (14.46 ± 11.92 pmol L–1 in control group and 14.09 ± 11.52 pmol L–1 in intervention group). After the intake of vitamin D supplementation in intervention group, AMH levels were increased to 24.89 ± 12.47 pmol L–1, which were significantly different from the 15.43 ± 13.03 pmol L–1 in control group (P < 0.001). Correlation coefficients for AMH with pre-intervention and post-intervention vitamin D were r = 0.489 and r = 0.599 respectively (P < 0.001). Treatment of vitamin D deficiency increases AMH to the optimum levels. Vitamin D deficient women had low levels of AMH. These findings support other studies which found a correlation of poor IVF outcomes with low vitamin D levels. Vitamin D supplementation could be useful in the improvement of controlled ovarian hyper-stimulation/IVF outcomes in case of vitamin D deficiency.

scholarly journals Correlation between the Lunar Phase and Tail-Lifting Behavior of Lizards (Pogona vitticeps) Exposed to an Extremely Low-Frequency Electromagnetic Field

Animals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 208 ◽  
Author(s):  
Tsutomu Nishimura ◽  
Harue Tada ◽  
Masanori Fukushima
Keyword(s):  
Magnetic Field ◽  
Full Moon ◽  
Low Frequency ◽  
Control Group ◽  
Lunar Phase ◽  
Extremely Low Frequency ◽  
K Index ◽  
Field Sensitivity ◽  
Pogona Vitticeps ◽  
New Moon

We previously showed that the agamid lizard Pogona vitticeps responded to an extremely low-frequency electromagnetic field (ELF-EMF; frequency: 6 and 8 Hz; peak magnetic field: 2.6 µT; peak electric field: 10 V/m) with tail-lifting behavior. In addition, the tail-lifting response to ELF-EMF disappeared when the parietal eyes of the lizards were covered by small round aluminum caps. This result suggests that the parietal eye contributes to light-dependent magnetoreception. In the present study, we set up an ELF-EMF group to evaluate the long-term effect of the ELF-EMF on lizards’ behavior and examine our hypothesis that exposure to ELF-EMFs increases the magnetic field sensitivity in lizards. We therefore include the lunar phase (full moon/new moon) and K index as environmental factors related to the geomagnetic field in the analysis. The number of tail lifts per individual per day was the response variable while calendar month, daily mean temperature, daily mean humidity, daily mean atmospheric pressure, full moon, new moon, and K index were the explanatory variables. We analyzed an ELF-EMF group and a control group separately. In a multiple linear regression analysis, the independent determinants associated with the number of tail lifts were the full moon, the temperature, February, March, April, and May in the ELF-EMF group and March, April, May, and June in the control group. The P. vitticeps in the ELF-EMF group responded to the full moon whereas those in the control group did not. In addition, in the ELF-EMF group, the number of tail lifts was higher on days when the K index was higher (P = 0.07) in the first period whereas there was no such tendency in either period in the control group. There is the possibility that the exposure to ELF-EMFs may increase magnetic-field sensitivity in lizards.

USE OF FEMOFLOR-16 TEST TO ASSESS GENITAL BIOCENOSIS IN WOMEN WITH INFLAMMATORY AND PROLIFERATIVE DISEASES OF CERVIX

2016 ◽  
Vol 1 (61) ◽  
pp. 90-95
Author(s):  
Иевлева ◽  
Nadezhda Ievleva ◽  
Пермина ◽  
Natalya Permina ◽  
Ивахнишина ◽  
...  
Keyword(s):  
Genital Tract ◽  
Inflammatory Diseases ◽  
Female Genital Tract ◽  
Reproductive Age ◽  
Gardnerella Vaginalis ◽  
Control Group ◽  
Healthy Women ◽  
Opportunistic Bacteria ◽  
Obligate Anaerobic ◽  
Atopobium Vaginae

Qualitative and quantitative assessment of microbes making the microbiocenosis of genital tract in women with inflammatory and proliferative diseases of cervix using Femoflor-16 test was the aim of the research. Scrapings of cervix and vaginal fornix in 100 women of reproductive age with cervicitis, vaginitis and in 31 women with cervical pseudoerosion (ectropion) were studied. The control group consisted of 35 relatively healthy women preparing for pregnancy. Cervical and vaginal dysbiosis was found in women with inflammatory diseases of cervix in 37.0% of cases, in women with pseudoerosion in 32.2% of cases. These are 3.3 (р&#60;0.005) and 2.8 (р&#60;0.02) times as much as in the group of healthy women preparing for pregnancy (11.1%). Dysbiosis structure was represented primarily by obligate anaerobic agents such as Gardnerella vaginalis, Atopobium vaginae, Eubacterium spp. in association with other opportunistic bacteria that are clinically most significant microorganisms colonizing female genital tract. Mycoplasma and yeast-like fungi of Candida species were found primarily with anaerobes. Aerobic and mixed dysbiosis were only found in 7% of cases in women with inflammatory diseases. Femoflor-16 test is a readily available, fast, efficient, up-to-date method enabling one to begin with early adequate antibacterial therapy and monitor it.

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