scholarly journals ANTIMICROBIAL PROPERTIES OF PLEUROTUS ERYNGII AND LENTINUS EDODES HYDRO-ALCOHOLIC EXTRACTS

2016 ◽  
Vol 73 (2) ◽  
pp. 259
Author(s):  
Gabriela Popa ◽  
Catalina Voaides ◽  
Petruta Cornea ◽  
Valentin Zagrean
Keyword(s):  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Pleurotus Eryngii ◽  
Diffusion Method ◽  
Lentinus Edodes ◽  
Disk Diffusion Method ◽  
Mushroom Species ◽  
Nutritional Values ◽  
Medicinal Properties ◽  
Ethanol Extracts

Besides superior nutritional values mushrooms posed significant medicinal properties. Hydro-alcoholic extracts of several isolates of Pleurotus eryngii and Lentinus edodes mushroom species were investigated for their antimicrobial activities against pathogenic microorganisms with medicinal importance. Antimicrobial activities of the extracts were evaluated by the agar disk diffusion method. Results revealed that the 70% ethylic alcohol extracts have significant inhibitory activities against Bacillus subtilis var. spizizinii, Escherichia coli and Staphylococcus aureus. The results showed that the 70% ethanol extracts of Pleurotus eryngii and Lentinus edodes mushroom isolates may have biopharmaceutical potentiality.

Comparative study of the ethanol extracts of six Acinos Miller species: chemical composition, antimicrobial and antioxidative activities

2020 ◽  
Vol 48 (1) ◽  
pp. 53-65
Author(s):  
Tatjana D. GOLUBOVIĆ ◽  
Gordana S. STOJANOVIĆ ◽  
Dušanka V. KITIĆ ◽  
Bojan K. ZLATKOVIĆ ◽  
Dragana R. PAVLOVIĆ ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Salmonella Enteritidis ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Fungal Organisms ◽  
Ethanol Extracts

The ethanol extracts of selected Acinos Miller species were investigated in terms of chemical composition, antimicrobial and antioxidative activities. Qualitative and quantitative analyses of the extracts were performed using GC and GC-MS. Eighty-six constituents, accounting for 93.70-99.99% of the total composition of the extracts, were identified. The ethanol extracts of A. majoranifolius, A. suaveolens and A. alpinus were characterized by domination of monoterpenes, representing 85.03%, 57.39% and 28.02% of the total extracts, respectively. Fatty acids and their esters in the A. arvensis and A. graveolens extracts reached 28.97% and 30.75%. Also, n-alkanes were the major compounds found in A. hungaricus ethanol extract (30.98%). The extracts were characterized by determination of total polyphenols, flavonoids and tannins. Besides, the antioxidant activity of the investigated extracts was estimated by two assays: DPPH and FRAP test. The highest antioxidant activity was observed in the extract of A. alpinus which had high levels of all examined polyphenol classes. A disk diffusion method was employed for the determination of the antimicrobial activities of the ethanol extracts. Gram-positive: Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Clostridium pyogenes, Enterococcus sp., Micrococcus flavus; Gram-negative: Klebsiella pneumoniae, Pseudomonas aerugionsa, Salmonella enteritidis, Proteus vulgaris, Escherichia coli and fungal organisms: Aspergillus niger, Candida albicans and Saccharomyces cerevisiae were used as test microorganisms. The results of preliminary bioassay demonstrated that the A. alpinus ethanol extract could be a possible source of compounds with antioxidant and antimicrobial activity.

scholarly journals ANTIMICROBIAL EFFECTS OF SOME DICOTYLEDONOUS PLANTS ON FUNGAL ISOLATES OF CANDIDA albicans AND TRICHOPHYTON mentagrophyte

2021 ◽  
Vol 4 (1) ◽  
pp. 1-4
Author(s):  
Obhioze Augustine Akpoka ◽  
A. A. Enaigbe ◽  
M. U. Okwu ◽  
O. E. Izevbuwa ◽  
E. A. Ufuah
Keyword(s):  
Candida Albicans ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Sensitivity Test ◽  
Diffusion Method ◽  
Leaf Extracts ◽  
Disk Diffusion Method ◽  
Fungal Isolates ◽  
Drug Concentrations

The preliminary phytochemical screening revealed that, antimicrobial properties of the leaf extracts were due to secondary metabolites such as amino acids, essential oils, flavonoids and saponins contained. The antimicrobial activities of alcoholic extracts were tested against pathogenic fungal isolates of Candida albicans and Trichophyton mentagrophyte. This was performed by inoculating the isolates into the pure extract, spread onto petri plates containing Sabouraud dextrose agar (SDA) media, observed for growth at stipulated standards. The sensitivity test was done by the disk diffusion method to test the effectiveness of an antimycotic (Griseofulvin) applied on the specific isolates. The minimum inhibitory concentration (MIC) was determined to ascertain the lowest drug concentrations that inhibited the fungal growths. The antimicrobial test revealed that, the leaf extracts of Eupatorium odoratum and Canjanus cajan inhibited the growths of the organisms while extracts of Citrus aurantifolia and Eucalyptus citriodora only prevented the growth of Candida albicans. The sensitivity test recorded the inhibition zone to range from 11 mm to 32 mm, with the lowest cleared area reported in the extract of E. citriodora and the highest in E, odoratum. Consequently, the MIC values of extracts at dilution levels were; E. odoratum: 1: 10000; 1: 1000, C. cajan: 1: 1000; 1: 10000, E. citriodora: 1:1000; 1:100 and C. aurantifolia: 1: 100000; 1: 100 respectively. This work has confirmed the progressive utilization of plants as antimicrobials for the benefit of mankind, to have originated from microbial sources.

scholarly journals In vitro antimicrobial activity of douro wines against clinical Helicobacter Pylori Strains

Nova ◽  
2021 ◽  
Vol 19 (37) ◽  
pp. 121-134
Author(s):  
Lidia Po Catalao Dionisio ◽  
Alejandro Manuel Labella ◽  
María Palma ◽  
Juan José Borrego
Keyword(s):  
Antimicrobial Activity ◽  
Helicobacter Pylori ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Horse Blood ◽  
H Pylori

Aim. In vitro antimicrobial activities of seven wines (5 reds and 2 whites) from the Douro region (Iberian Peninsule) against eleven clinical strains of Helicobacter pylori were evaluated. Methods. The disk diffusion method, using Columbia Agar supplemented with horse blood (CAB), were used to determine the antimicrobial properties of some wine components against H. pylori strains. Potential interactions of antioxidants contained in the wines and two antimicrobials (amoxicillin and metronidazole) were studied by the disk diffusion method. Results. All the tested strains showed growth in CAB supplemented with 9% of the tested wines but none of them grew in media supplemented with 45% and 67.5% of wine. Similarly, all the tested strains grew in media with the concentration of proanthocyanidins present in the different types of the studied wines. The Minimal Inhibitory Concentration (MIC) values of the wine antioxidant components tested (benzoic acid, catechin, quercetin, and resveratrol) indicate that resveratrol was the most powerful inhibitory substance against H. pylori. An effect of potentiation between amoxicillin and metronidazole and the antioxidants tested was also established. The interaction of amoxicillin and resveratrol or metronidazole and catechin increased the antimicrobial activity against H. pylori. Conclusions. The results obtained suggested a potential role of resveratrol as a chemopreventive agent for H. pylori infection.

scholarly journals Comparative Study of Non Polar and Polar Solvent Extracts of Glochidion Velutinum Wight in Respect of Antioxidant and Antibacterial Action

2018 ◽  
Vol 41 (2) ◽  
pp. 247-252
Author(s):  
Md Selim Hossain ◽  
Ananta Kumar Das ◽  
Fazlay Rabbi ◽  
Mesbahuddin Ahmad
Keyword(s):  
Antioxidant Activities ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Disk Diffusion Method ◽  
Methanol Extracts ◽  
Ethanol Extracts ◽  
Free Radical Scavenging Assay

Antioxidant and antimicrobial activities of non-polar and polar extracts of Glochidion velutinum Wight have been studied. The phenolic content was determined by Folin-Ciocalteu reagent and colorimetric method was used to quantify flavonoids. Antioxidant activity was also measured by DPPH free radical scavenging assay and disk diffusion method was applied to test antimicrobial activity. Of the hexane, chloroform, ethanol and methanol extracts of Glochidion velutinum, higher total phenolic and flavonoid contents were found in methanol and ethanol extracts. Moreover, ethanol and methanol extracts also showed better antimicrobial and antioxidant activities than the other two extracts. The presence of various phytochemicals e.g. alkaloids, tannins, phenols etc in these extracts also substantiated the observed antioxidant and antimicrobial activities.Journal of Bangladesh Academy of Sciences, Vol. 41, No. 2, 247-252, 2017

scholarly journals Chemical composition and screening of the antimicrobial and anti-oxidative activity of extracts of Stachys species

2010 ◽  
Vol 75 (10) ◽  
pp. 1347-1359 ◽  
Author(s):  
Jelena Lazarevic ◽  
Radosav Palic ◽  
Niko Radulovic ◽  
Novica Ristic ◽  
Gordana Stojanovic
Keyword(s):  
Ethyl Acetate ◽  
Diethyl Ether ◽  
Salmonella Enteritidis ◽  
Balkan Peninsula ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Diethyl Ether Extract ◽  
The Common ◽  
Ethyl Acetate Extracts

GC and GC/MS analyses of the diethyl ether and ethyl acetate extracts obtained from the aerial parts of Stachys germanica subsp. heldreichii (Boiss) Hayek, Stachys iva Griseb., Stachys plumosa Griseb. and Stachys scardica Griseb., Balkan peninsula endemics, were performed. One hundred and seventy-nine constituents, accounting for 88.8-98.1% of the total composition of the extracts, were identified. The common feature of the diethyl ether extracts was the high content of terpenoids and fatty acidderived compounds, while the common feature of the ethyl acetate extracts was the prevalence of fatty acid-derived compounds. A disk diffusion method was used for the evaluation of the antimicrobial activities of the extracts against a panel of microorganisms (bacteria: Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Salmonella enteritidis; fungi: Aspergillus niger and Candida albicans). The total antioxidant capacity of the extracts was evaluated by the phosphomolybdenum method. The preliminary bioassay results indicated that the diethyl ether extract of S. plumosa could be a possible source of antioxidant and antimicrobial compounds.

scholarly journals Evaluating the Antimicrobial Activity of Commercially Available Herbal Toothpastes on Microorganisms Associated with Diabetes Mellitus

2013 ◽  
Vol 14 (5) ◽  
pp. 924-929 ◽  
Author(s):  
Reena Kulshrestha ◽  
J Kranthi ◽  
P Krishna Rao ◽  
Feroz Jenner ◽  
V Abdul Jaleel ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Antimicrobial Activity ◽  
Dental Health ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Test Organism ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Disk Diffusion Method

ABSTRACT Aim The present study was conducted to evaluate the efficacy of commercially available herbal toothpastes against the different periodontopathogens. Materials and methods Six herbal toothpastes that were commonly commercially available were included in the study. Colgate herbal, Babool, Meswak, Neem active, Dabur red toothpastes were tested for the study whereas sterile normal saline was used as control. Antimicrobial efficacies of dentifrices were evaluated against Streptococcus mutans and Actinobacillus actinomycetemcomitans. The antimicrobial properties of dentifrices were tested by measuring the maximum zone of inhibition at 24 hours on the Mueller Hinton Agar media inoculated with microbial strain using disk diffusion method. Each dentifrice was tested at 100% concentration (full strength). Results The study showed that all dentifrices selected for the study were effective against the entire test organism but to varying degree. Neem active tooth paste gave a reading of 25.4 mm as the zone of inhibition which was highest amongst all of the test dentifrices. Colgate Herbal and Meswak dentifrices recorded a larger maximum zone of inhibition, measuring 23 and 22.6 mm respectively, compared to other toothpastes. All other dentifrices showed the zone of inhibition to be between 17 and 19 mm respectively. Conclusion The antibacterial properties of six dentifrices were studied in vitro and concluded that almost all of the dentifrices available commercially had antibacterial properties to some extent to benefit dental health or antiplaque action. How to cite this article Jenner F, Jaleel VA, Kulshrestha R, Maheswar G, Rao PK, Kranthi J. Evaluating the Antimicrobial Activity of Commercially Available Herbal Toothpastes on Microorganisms Associated with Diabetes Mellitus. J Contemp Dent Pract 2013;14(5):924-929.

scholarly journals The PHYTOCHEMICALS, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Senna alata AND Senna tora LEAF EXTRACTS AGAINST BACTERIAL STRAINS CAUSING SKIN INFECTIONS

2019 ◽  
Vol 2 (1) ◽  
pp. 19
Author(s):  
Murni Halim
Keyword(s):  
Leaf Extract ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Natural Drugs ◽  
Senna Alata

A study was carried out to screen for phytochemical constituents and assess the antioxidant and antimicrobial activities of Senna alata and Senna tora leaf extracts. The leaves were first dried at room temperature and 50°C in an oven prior to solvent extraction using ethanol and methanol. The in-vitro qualitative assays showed that both S. alata and S. tora leaf extracts contained bioactive and secondary metabolites components such as tannins, steroids, saponin, terpenoids, glycosides, flavonoids and phenols. The antioxidant activity and capacity test were carried out by conducting free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Ferric reduction antioxidant plasma (FRAP) assays. Both assays showed S. tora leaf extract has higher antioxidant capacity than S. alata leaf extract. The efficacy of these leaf extracts were tested against skin pathogens through agar well diffusion method. S. alata extract showed an inhibition zone (1.15 – 1.59 mm) against Pseudomonas aeruginosa while S. tora extracts exhibited a strong antimicrobial activity against S. epidermidis (inhibition zone of 12 – 16.94 mm) followed by P. aeruginosa (inhibition zone of 1 – 1.59 mm). Nonetheless, no inhibition zone was observed for S. aureus by both leaf extracts. The phytochemicals and antioxidant constituents as well as inhibitory potential on skin pathogens possessed by S. alata and S. tora leave highlighted their potential utilization in the development of natural drugs or cosmetics to treat skin related diseases or infections.

scholarly journals Antimicrobial Activity of Cultivable Endophytic and Rhizosphere Fungi Associated with “Mile-a-Minute,” Mikania cordata (Asteraceae)

2020 ◽  
Vol 2020 ◽  
pp. 1-7 ◽  
Author(s):  
Pavithra L. Jayatilake ◽  
Helani Munasinghe
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Pharmaceutical Products ◽  
Diffusion Method ◽  
Molecular Characteristics ◽  
Disk Diffusion Method ◽  
Rhizosphere Fungi

Endophytic and rhizosphere fungi are understood to be aiding the host plant to overcome a range of biotic and abiotic stresses (nutrition depletion, droughts, etc.) hence, they remain to be reservoirs of plethora of natural products with immense use. Consequently, this investigation of endophytic and rhizosphere fungi isolated from Mikania cordata (a perennial vine that is well established in Sri Lanka) for their antimicrobial properties was performed with the aim of future derivation of potential beneficial pharmaceutical products. Leaves, twigs, and roots of M. cordata were utilized to isolate a total of 9 endophytic fungi out of which the highest amount (44%) accounted was from the twigs. A sample of the immediate layer of soil adhering to the root of M. cordata was utilized to isolate 15 rhizosphere fungi. Fusarium equiseti and Phoma medicaginis were endophytes that were identified based on colony and molecular characteristics. The broad spectrum of antimicrobial activity depicted by F. equiseti (MK517551) was found to be significantly greater (p≤0.05, inhibitory against Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 25853) than P. medicaginis (MK517550) (inhibitory against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 25853) as assessed using the Kirby-Bauer disk diffusion method. Trichoderma virens and Trichoderma asperellum were rhizospere fungi that exhibited remarkable antimicrobial properties against the test pathogens chosen for the study. T. asperellum indicated significantly greater bioactivity against all four bacterial pathogens and Candida albicans ATCC 10231 under study. The ranges of minimum inhibitory concentrations (MICs) of the fungi depicting antimicrobial properties were determined. The results obtained suggest that F. equiseti, P. medicaginis, T. asperellum, and T. virens of M. cordata harness bioprospective values as natural drug candidates. This is the first report on isolation and evaluation of the antimicrobial properties of endophytic and rhizosphere fungi of Mikania cordata.

Antimicrobial activity of raw soybean, soybean flour and roasted soybean extracted by ethanol-hexane method

2017 ◽  
Vol 119 (10) ◽  
pp. 2277-2286 ◽  
Author(s):  
Sireerat Laodheerasiri ◽  
Nirasha Horana Pathirage
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Antimicrobial Compounds ◽  
Soybean Flour ◽  
Hexane Extraction ◽  
Disk Diffusion Method ◽  
Content Type ◽  
E Coli ◽  
Inhibitory Compounds

Purpose The purpose of this paper is to determine the antimicrobial compounds extracted from various types of soybean materials; raw soybean, soybean flour and roasted soybean. Bacteria growth inhibition was tested in E.coli and S. aureus by disk diffusion method. Efficiency of antimicrobial activities were compared among the extracted solution. Design/methodology/approach Soybean (Glycine max) isoflavones contain the ability to inhibit the growth of many strains of microorganisms. The solid-liquid extraction, ethanol-hexane extraction, was modified to isolate the inhibitory compounds from the three different types of soybean materials. All crude extracts at various concentrations performed under different extracted solutions (75, 50, 25, 12.5, 6.25 and 3.125 percent) were tested with E. coli. and S. aureus to determine the antimicrobial activities and the minimum inhibition concentration (MIC). Disk diffusion method was chosen to study the antimicrobial activity of isoflavones. Findings Soybean flour extract inhibited the growth of E. coli and S. aureus more effective than roasted soybean and raw soybean, respectively. The MIC of inhibitory compounds extracted from soybean flour was 6.25 percent (0.031 mg/ml), roasted soybean was 25 percent (0.125 mg/ml) and raw soybean was 50 percent (0.250 mg/ml). Crude extracts of soybean flour and roasted soybean showed better results than raw soybean. All the three of extracted soybeans inhibited the growth of E. coli better than S. aureus. Originality/value Ethanol-hexane extraction was successfully used to isolate the antimicrobial compounds from raw soybean, soybean flour and roasted soybean. Comparison of antimicrobial activity showed that soybean flour contained the highest inhibition activity.

scholarly journals Bazı Çiğ Olarak Tüketilen Mor Sebzelerin Etanol Ekstraktlarının Gıda Kaynaklı Küfler Üzerindeki Antifungal Etkileri

2020 ◽  
Vol 8 (2) ◽  
pp. 436
Author(s):  
Gökhan Akarca ◽  
Oktay Tomar ◽  
Elif Başpınar ◽  
Gamze Yıldırım
Keyword(s):  
Ethanol Extract ◽  
Geotrichum Candidum ◽  
Diffusion Method ◽  
Mineral Matter ◽  
Disk Diffusion Method ◽  
Antifungal Effect ◽  
Black Carrot ◽  
Zone Diameter ◽  
Red Beet ◽  
Ethanol Extracts

Vegetables; in terms of vitamins, nutritional fiber, phenolic component and mineral matter has an important role in human nutrition. There are many species of purple-red vegetables, some of which are well-known, such as red beet (Beta vulgaris), black carrot (Daucus carota ssp. sativus var. atrorubens Alef), black radish (Raphanus sativus L. var. niger), purple cabbage (Brassica oleracea L. var. capitata f. rubra). Purple or red vegetables have higher antioxidant potential compared to other vegetables, which have many beneficial properties such as antimicrobial, antifungal, anti-inflammatory and liver protection. Each of vegetables which is black carrot, beetroot, black radish and purple cabbage contains p-coumaric acid, betaxanthin, ellagic acid and indole-3-carbinol respectively. In this study, Antifungal effect of ethanol extracts obtained from red beet, black carrot, black radish which are inner and peel part, purple cabbage against 7 different subspecies of Penicillium, 6 different subspecies of Aspergillus and Mucor racemosus, Botrytis cinerea, Geotrichum candidum, Cladosporium claudosporioides, Rhizopus nigricans species were determined by using disk diffusion method. As a consequence; it was determined that 7 different samples had antifungal effect on 18 different mold species at various rate. The highest antifungal effect was observed with 23.05±0.05 mm zone diameter against A. fumigatus in black radish inner part ethanol extract. This value was pursued by red beet inner part ethanol extract against M. racemosus with 21.44±0.12 mm zone diameter. It was concluded that the lowest antifungal effect on mold species except for P. citrinum, P. solitum and B. cinerea in purple cabbage extract.

Sign in / Sign up

Export Citation Format

Share Document