scholarly journals Methods of Detecting Meat Species in Food of Animal Origin

2018 ◽  
Vol 75 (2) ◽  
pp. 125
Author(s):  
Lavinia-Maria CHIŞ ◽  
Dan-Cristian VODNAR
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Meat Product ◽  
Meat Products ◽  
Ease Of Use ◽  
Animal Origin ◽  
Processed Meat ◽  
Elisa Method ◽  
Food Of Animal Origin ◽  
Pcr Method

An important factor in the detection of falsification is the control of the composition of the meat at each stage of manufacturing the product. The PCR method is based on the study of proteins and meat nucleic acids used in food for the detection of animal species. Another technique is the Elisa method that works on the principle of identification and measurement of the quantity of molecules in a sample. There are several types of Elisa to increase specificity due to differences in structure and sample characteristics. By comparing the two methods used to identify the processed meat product species, Real Time PCR had the highest prediction as results. However, the Elisa method is more time efficient and easier to use. Real Time PCR is effective in identifying processed meat products that require low detection. The Elisa Kit is useful because of the ease of use.

scholarly journals Short Communication: Detection of Salmonella typhimurium ATCC 14028 and Listeria monocytogenes ATCC 7644 in processed meat products using Real-Time PCR Multiplex Method

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Alfi Sophian ◽  
RATNA PURWANINGSIH ◽  
EKA PUTRI JUNIARTI IGIRISA ◽  
MUHAMMAD LUTHFI AMIRULLAH ◽  
BERTHA LOLO LUKITA ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Real Time ◽  
Melting Temperature ◽  
Real Time Pcr ◽  
Meat Products ◽  
Processed Meat ◽  
Ct Value ◽  
Pcr Multiplex ◽  
Pcr Method

Abstract. Sophian A, Purwaningsih R, Igirisa RPJ, Amirullah ML, Lukita BL, Fitri RA. 2020. Short Communication: Detection of Salmonella typhimurium ATCC 14028 and Listeria monocytogenes ATCC 7644 in processed meat products using Real-Time PCR Multiplex Method. Asian J Nad Prod Biochem 21: 17-20. The detection of Salmonella typhimurium ATCC 14028 and Listeria monocytogenes ATCC 7644 in processed meat products was carried out using Multiplex Real-Time PCR (qPCR) in the Microbiology and Molecular Biology Laboratory at the Indonesian Food and Drug Authority in Gorontalo. The purpose of this study was to provide alternative testing methods for food products circulating in the market. The sample consisted of 25 samples of processed meat products spike with Salmonella typhimurium ATCC 14028 phase 2 and Listeria monocytogenes ATCC 7644 phase 2. The method used in the study was qPCR analysis using the SYBR Green method, while DNA isolation used the direct PCR method. Data analysis was carried out based on Cycle threshold and Melting temperature based on two main criteria. Cycle threshold (Ct) analysis determines the Ct value of the sample and comparing it with the control. Melting temperature (Tm) analysis determines the temperature at which 50% of double-stranded DNA changed to a single standard and comparing it with the melting temperature of positive control. The results showed Salmonella typhimurium ATCC 14028 in the processed meat was detected at an average Ct value of 10.34, and a Tm value of 85.70. The presence of Listeria monocytogenes ATCC 7644 in the samples was recognized at an average Ct value of 14.04, and an average Tm value of 80.07. It can be concluded that the real-time multiplex PCR method can be used to detect Salmonella typhimurium ATCC 14028 and Listeria monocytogenes ATCC 7644 by using the melting curve (Tm) analysis.

A Fast and Reliable Real-Time PCR Method for Detection of Ten Animal Species in Meat Products

2018 ◽  
Vol 83 (2) ◽  
pp. 258-265 ◽  
Author(s):  
Lissandra Sousa Dalsecco ◽  
Rafael Melo Palhares ◽  
Pollyana Carvalho Oliveira ◽  
Lilian Viana Teixeira ◽  
Marcela Gonçalves Drummond ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Animal Species ◽  
Meat Products ◽  
Pcr Method

scholarly journals REAL TIME POLYMERASE CHAIN REACTION (RT-PCR) FOR DNA PIG CONTAMINATION IDENTIFICATION SAUSAGE IN SIX DISTRICT PANDEGLANG BANTEN

2021 ◽  
Vol 1 (1) ◽  
pp. 81-88
Author(s):  
Hadi Susilo
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Meat Product ◽  
Meat Products ◽  
Pcr Analysis ◽  
Processed Meat ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Dna Purity ◽  
Polymerase Chain

Sausage is a meat product processed that is popular food especially in Pandeglang, Banten Province. The importance of halal certificates or the existence of the MUI (Indonesian Ulama Council) halal logo for processed meat products makes Muslim people confident to consume them. The aim this research was to identify pig DNA contamination in sausage products in six  districts in Pandeglang without the MUI halal labels using RT-PCR (Real Time-Polymerase Chain Reaction). RT PCR that can calculate to pig to fill these sample free from pig contamination. This research was divided into two stage, the first stage is extracted or carried out DNA and the second stage is RT PCR analysis. The results of the DNA purity test on sausage samples had DNA purity values ​​of 1.84-1.9 (A260 / A280) and resulted in sample concentrations ranging from 37.8 to 102.5 ng / µl.  The only amplification on the FAM curve was in the positive control pig.  the Cq value ranges from 30 - 31.29. The results of RT PCR on sausage samples in the district area in Pandeglang Banten did not detect the presence of pig DNA.

scholarly journals Detection of meat from horse, donkey and their hybrids (mule/hinny) by duplex real-time fluorescent PCR

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0237077
Author(s):  
Dan Wang ◽  
Liping Wang ◽  
Chenyu Xue ◽  
Yuebei Han ◽  
Hejing Li ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Limit Of Detection ◽  
Meat Products ◽  
Processed Meat ◽  
Similar Species ◽  
Fluorescent Pcr ◽  
Species Specific ◽  
Muscle Gene ◽  
Fluorescence Amplification

Meat adulteration is currently a common practice worldwide. In China, adulteration of donkey meat products with the similar species (horse and mule/hinny) meat and mislabeling are becoming widespread concerns. In this study, a sensitive and species-specific duplex real-time PCR assay based on the simultaneous amplification of fragments of the creatine kinase muscle gene family, was developed and optimized for the identification of horse, donkey and mule /hinny species in raw and heat-processed meat products. Duplex real-time PCR results showed different fluorescence amplification curves for horse and donkey. Both kinds of fluorescence amplification curves appeared simultaneously for mule/hinny. The limit of detection (LOD) of the method was up to 0.01 ng /μL. The method and strategy developed in this study could be applied to detect the presence of adulterants from horse and mule /hinny meat in raw donkey meat and meat products.

Comparative Studies of a Real-Time PCR Method and Three Enzyme-Linked Immunosorbent Assays for the Detection of Central Nervous System Tissues in Meat Products†

2008 ◽  
Vol 71 (10) ◽  
pp. 2059-2066 ◽  
Author(s):  
HOLGER SCHÖNENBRÜCHER ◽  
KATRIN ANNETTE GÖBEL ◽  
AMIR ABDULMAWJOOD ◽  
JÜRGEN A. RICHT ◽  
MICHAEL BÜLTE
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Real Time ◽  
Real Time Pcr ◽  
Meat Products ◽  
Spongiform Encephalopathy ◽  
Pcr Assay ◽  
Heat Treated ◽  
Pcr Method ◽  
Minced Meat

The removal of certain central nervous system (CNS) tissues (part of the bovine spongiform encephalopathy risk material) from the food chain is one of the highest priority tasks associated with avoiding contamination of the human food chain with the agent of bovine spongiform encephalopathy. A recently developed real-time PCR assay and three commercially available enzyme-linked immunosorbent assays (ELISAs) for the detection of CNS tissues in minced meat and three types of heat-treated sausages were evaluated. Bovine brain was used for spiking of internal reference material, and its detectability was examined during storage times of 12 months (for frozen minced meat and liver sausage) and 24 months (for sausages treated with medium and high heat). The real-time PCR method and both ELISA kits detected 0.1% CNS tissue in frozen minced meat and 0.1 or 1% CNS tissue in heat-treated meat products. The detectability of the amplified mRNA target region with the PCR assay was similar to the detectability of antigen by the ELISAs. Because the real-time PCR method also can be used to distinguish cattle, ovine, and caprine CNS tissues from porcine CNS tissues, it seems to be suitable as a routine diagnostic test for the sensitive and specific detection of CNS tissues in meat and meat products.

scholarly journals Detection and Quantification of Milk Ingredients as Hidden Allergens in Meat Products by a Novel Specific Real-Time PCR Method

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 804 ◽  
Author(s):  
Caterina Villa ◽  
Joana Costa ◽  
Isabel Mafra
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Limit Of Detection ◽  
Meat Products ◽  
Milk Proteins ◽  
12S Rrna ◽  
Processed Meat ◽  
Rrna Gene ◽  
Wide Range ◽  
Detection And Quantification

Milk ingredients are often included in a wide range of meat products, such as cooked hams and sausages, to improve technological characteristics. However, milk proteins are also important food allergens. The aim of this study was the development of a highly sensitive and specific real-time PCR system targeting the 12S rRNA gene of Bos domesticus for the detection and quantification of milk as an allergenic ingredient in processed meat products. The method was able to achieve an absolute limit of detection (LOD) of 6 fg of milk DNA. Using a normalized approach (∆Ct method) for the detection of milk protein concentrate (MPC), it was possible to obtain sensitivities down to 0.01% (w/w) of MPC in model hams (raw and cooked) and autoclaved sausages, and 0.005% in raw sausage mixtures. The developed systems generally presented acceptable PCR performance parameters, being successfully validated with blind samples, applied to commercial samples, and further compared with an immunochemical assay. Trace amounts of milk material were quantified in two out of 13 samples, but the results mostly infer the excessive practice of the precautionary labeling.

scholarly journals Detection of the Species of Origin for Pork, Chicken and Beef in Meat Food Products by Real-Time PCR

Safety ◽  
2019 ◽  
Vol 5 (4) ◽  
pp. 83
Author(s):  
Lavinia-Maria Chiş ◽  
Dan Cristian Vodnar
Keyword(s):  
Human Health ◽  
Real Time ◽  
Real Time Pcr ◽  
Food Products ◽  
Health Protection ◽  
Industrial Safety ◽  
Animal Origin ◽  
Processed Meat ◽  
Safety Control ◽  
Meat Species

Processed food products of animal origin raise questions related to industrial safety and human health protection. This paper aimed to optimize and validate a real-time, sensitive, and accurate PCR method for the detection and quantification of meat species in selected processed meat products: chicken sausages, beef bologna, and pork bologna. A common detection limit of 8 DNA copies was established for each sample, corresponding to 0.1% for beef and pork and 0.2% for chicken. For the limit of quantification, dilutions of 20 copies of DNA for the bovine and pig species and 50 copies of DNA for the chicken species were performed. Specificity and selectivity tests in six replicates each showed no extraneous meat species, in line with the label. Repeatability was assessed in six replicates, both quantitatively and qualitatively, by the same analyst, on the same day, and with the same equipment. The results showed that beef bologna contained 84.49% beef meat, pork bologna 92.8% pork meat, and chicken sausages 95.14% chicken meat. The reproducibility results obtained by two analysts, on different days, for each sample were very similar. The real-time PCR technique can be used as a tool in internal and public safety control to improve industrial safety and human health protection.

scholarly journals Application of real-time PCR for DNA identification of ruminants in raw animal products

2018 ◽  
Vol 74 (1) ◽  
pp. 6061-2018
Author(s):  
ANNA WEINER ◽  
ILONA PAPROCKA ◽  
AGATA GOŁĘBIOWSKA ◽  
KRZYSZTOF KWIATEK
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Real Time Pcr ◽  
Animal Origin ◽  
Dna Identification ◽  
Animal Products ◽  
Modified Method ◽  
Pcr Method ◽  
Processed Products ◽  
By Products

According with current regulations it is possible export of processed animal proteins produced from tissues other animals than ruminants. In order to ensure appropriate control studies were undertaken to adopt of method real-time PCR used for analysis of processed products of animal origin. The material consisted of raw animal by-products of beef, pork and poultry. In the assays mitochondrial DNA was used. Modified method allows to detect the DNA of ruminants at the level of 0.025%. Based on obtained results of the validation studies were found that the real-time PCR method can be used in routine tests for identification of ruminant DNA in raw animal by-products..

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