scholarly journals Infrared Light-Emitting Diode (LED) Effects on Orthodontic Tooth Movement

2019 ◽  
Vol 30 (4) ◽  
pp. 410-416 ◽  
Author(s):  
Simone Peixe Friedrichsdorf ◽  
Victor Elias Arana-Chavez ◽  
Vivian Bradaschia-Correa ◽  
Paolo Maria Cattaneo ◽  
Gladys Cristina Dominguez
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Light Emitting Diode ◽  
Histological Study ◽  
Orthodontic Tooth Movement ◽  
Infrared Light ◽  
Tartrate Resistant Acid Phosphatase ◽  
Micro Computed Tomography ◽  
Led Phototherapy ◽  
Significant Difference

Abstract The present study aimed to analyze the effect of LED phototherapy on the presence of hyalinization and root resorption during orthodontic tooth movement (OTM) in rats and to measure the amount of tooth movement. Eighty rats were allocated into two groups: LED and control (CON), where the LED rats were irradiated with infrared LED (850 nm, 30 mW) for 5 min during the first five days of OTM and where controls were not irradiated. Both groups were subdivided into four subgroups (n=10) according to the date of euthanasia (4, 7, 14 and 21 days). Five out of ten LED21 and five of ten CON21 rats were submitted to micro-computed tomography (μCT); μCT scans were taken on days 0, 7, 14 and 21. For histological study, maxillae were processed to light microscopy using Hematoxylin-Eosin (HE) and Tartrate-Resistant Acid Phosphatase (TRAP) histochemistry. The amount of tooth movement did not differ between LED and CON. Hyalinization was observed at the pressure areas in both groups, and it did not show a statistically significant difference between the groups. Root resorption was also observed in both groups after 7 days and it did not represent any differences between the two groups. LED phototherapy was not able to increase the amount of OTM. Similar characteristics of hyalinization and root resorption were observed in both groups.

scholarly journals The effect of alveolar decortication on orthodontically induced root resorption

2020 ◽  
Vol 90 (4) ◽  
pp. 524-531 ◽  
Author(s):  
Po-Jung Chen ◽  
Joy H. Chang ◽  
Eliane H. Dutra ◽  
Ahmad Ahmida ◽  
Ravindra Nanda ◽  
...  
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase ◽  
Micro Computed Tomography ◽  
Root Volume ◽  
Male Wistar Rats ◽  
Group 3 ◽  
Group 2

ABSTRACT Objective To determine the effect of alveolar decortication on orthodontically induced root resorption. Materials and Methods A total of 24 male Wistar rats (14 week old) were used. The rats were randomly divided into one of the following three groups: group 1 (control group), orthodontic tooth movement (OTM) for 2 weeks; group 2, OTM for 2 weeks + two alveolar decortications (2AD); group 3, OTM for 2 weeks + four alveolar decortications (4AD). The first molar was moved mesially for 2 weeks. Micro computed tomography was used to analyze root volume. In addition, histological sections were stained with Tartrate Resistant Acid Phosphatase (TRAP) to quantify the osteoclast number. Results The buccal root volume in OTM + 4AD group was decreased by 8.92% and 6.11% when compared with the OTM-only group and OTM + 2AD group, respectively. Similarly, the other four root volumes in the OTM + 4AD group was decreased by 8.99% and 5.24% when compared with the OTM-only group and OTM + 2AD group, respectively. There was a decrease in buccal root density in the OTM + 4AD group by 4.66% and 3.56% when compared with the OTM-only group and the OTM + 2AD group, respectively. In addition, there was an increase in the number of osteoclasts by 195.73% and 98.74% in OTM + 4AD group in comparison with the OTM and OTM + 2AD group. Conclusions The amount of orthodontically induced root resorption was positively correlated with the extent of surgical injury used to accelerate orthodontic tooth movement.

scholarly journals Phototherapy is unable to exert beneficial effects on orthodontic tooth movement in rat molars

2019 ◽  
Vol 89 (6) ◽  
pp. 936-941 ◽  
Author(s):  
Simone P. Friedrichsdorf ◽  
Emily Zaniboni ◽  
Alyne Simões ◽  
Victor E. Arana-Chavez ◽  
Gladys C. Dominguez
Keyword(s):  
Laser Irradiation ◽  
Tooth Movement ◽  
Root Resorption ◽  
Light Emitting Diode ◽  
Orthodontic Tooth Movement ◽  
Male Rats ◽  
Light Emitting ◽  
Beneficial Effects ◽  
Infrared Laser Irradiation ◽  
Rat Molars

ABSTRACT Objectives: To investigate the effects of irradiation lasers and light-emitting diode (LED) light on root resorption in rat molars during orthodontic tooth movement (OTM). Materials and Methods: Twenty-one 12-week-old Wistar male rats were divided into three groups: OTM only (control [CG]), OTM and LED irradiation (DG), and OTM and low laser irradiation therapy (LG). The distance between the first and second molars was used to evaluate the amount of tooth movement. The mesial surfaces of the distopalatal roots were analyzed by scanning electron microscopy, and the area of the resorption was calculated. Results: Statistical analysis showed a decreased amount of tooth movement in the exposed DG or LG compared with CG, which was statistically significant (P = .031 and P = .004, respectively). However, when the DG and LG groups were compared, no statistically significant differences were found (P = .504). The root resorption areas were similar between CG and DG. However, statistically significant differences were found between LG and CG (P = .014) and LG and DG (P = .038). Conclusions: Phototherapy did not enhance tooth movement, while infrared laser irradiation did increase root resorption.

scholarly journals Automated tooth segmentation as an innovative tool to assess 3D-tooth movement and root resorption in rodents

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Viktoria Trelenberg-Stoll ◽  
Dieter Drescher ◽  
Michael Wolf ◽  
Kathrin Becker
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Orthodontic Tooth Movement ◽  
The Novel ◽  
Micro Computed Tomography ◽  
Novel Approach ◽  
Animal Trial ◽  
Innovative Tool ◽  
Upper Molar ◽  
Tooth Segmentation

Abstract Background Orthodontic root resorptions are frequently investigated in small animals, and micro-computed tomography (μCT) enables volumetric comparison. Despite, due to overlapping histograms from dentine and bone, accurate quantification of root resorption is challenging. The present study aims at (i) validating a novel automated approach for tooth segmentation (ATS), (ii) to indicate that matching of contralateral teeth is eligible to assess orthodontic tooth movement (OTM) and root resorption (RR), (iii) and to apply the novel approach in an animal trial performing orthodontic tooth movement. Methods The oral apparatus of three female mice were scanned with a μCT. The first molars of each jaw and animal were segmented using ATS (test) and manually (control), and contralateral volumes were compared. Agreement in root volumes and time efficiency were assessed for method validation. In another n = 14 animals, the left first upper molar was protracted for 11 days at 0.5 N, whereas the contralateral molar served as control. Following ATS, OTM and RR were estimated. Results ATS was significantly more time efficient compared to the manual approach (81% faster, P < 0.01), accurate (volume differences: − 0.01 ± 0.04 mm3), and contralateral roots had comparable volumes. Protracted molars had significantly lower root volumes (P = 0.03), whereas the amount of OTM failed to reveal linear association with RR (P > 0.05). Conclusions Within the limits of the study, it was demonstrated that the combination of ATS and registration of contralateral jaws enables measurements of OTS and associated RR in μCT scans.

scholarly journals Ultrasound enhances the healing of orthodontically induced root resorption in rats

2011 ◽  
Vol 82 (1) ◽  
pp. 48-55 ◽  
Author(s):  
Zhifeng Liu ◽  
Juan Xu ◽  
Lingling E ◽  
Dongsheng Wang
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Osteoclast Differentiation ◽  
Orthodontic Tooth Movement ◽  
Cell Number ◽  
Positive Control ◽  
Negative Control ◽  
Tartrate Resistant Acid Phosphatase ◽  
Activity Levels ◽  
Tissue Slices

Abstract Objective: To examine the effect of low-intensity pulsed ultrasound (LIPUS) on orthodontically induced root resorption in rats. Materials and Methods: Sixty-four male Wistar rats were divided randomly and equally into four groups (n  =  16 rats each). The rats were untreated (negative control) or treated with orthodontic tooth movement without (positive control) or with LIPUS at 100 or 150 MW/cm2 (LIPUS-treated groups). An initial force of 100 g was applied to the areas between the upper right central incisors and the first molars of the rats for 10 days. Eight rats were randomly chosen from each group, and the root resorption index (RRI) was determined with scanning electron microscopy (SEM). Upper first molar-centered mesial-distal tissue slices were generated from the upper first molars and peridentium of the remaining eight rats from each group. Specimen slices were analyzed with hematoxylin-eosin and tartrate-resistant acid phosphatase staining, osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) immunohistochemistry, and optical microscopy. Analyses of cell number, densitometry, and one-way analysis of variance were performed. Results: The LIPUS-treated groups displayed decreased RRI values, decreased osteoclast numbers and activity levels, and increased OPG/RANKL expression ratios. High-power SEM revealed reparative cementum in the LIPUS-treated samples. Conclusion: LIPUS regulates osteoclast differentiation via the OPG/RANKL ratio, evoking a reparative effect on orthodontically induced root resorption in rats.

scholarly journals Correlation of IL-17 level in gingival crevicular fluid of orthodontically induced inflammatory root resorption

2019 ◽  
Author(s):  
Hua Zhou ◽  
Xiao Liang ◽  
Aipeng Liu ◽  
Dongmei Nong ◽  
Yaqing Qin ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Tooth Movement ◽  
Root Resorption ◽  
Early Stage ◽  
Morphological Changes ◽  
Orthodontic Tooth Movement ◽  
Nickel Titanium ◽  
Tartrate Resistant Acid Phosphatase ◽  
Periodontal Tissues ◽  
Osteoclast Number

ABSTRACTObjectiveTo investigate IL-17 expression in orthodontic tooth movement and orthodontic nickel-titanium spring-induced inflammatory root resorption.MethodsOrthodontic nickel-titanium springs were ligated between the bilateral maxillary first molar and the incisors of the rats to establish a rat model of orthodontic tooth movement (OTM), each rat was subjected to two cycles of near-GCF and peripheral blood serum collection before and after force application, and IL-17 levels in GCF and serum were measured quantitatively by ELISA. Morphological changes in periodontal tissue and root of the experimental dentine were evaluated by hematoxylin and eosin staining. Tartrate-resistant acid phosphatase staining and immunohistochemistry were used to determine the osteoclast number and expression changes in IL-17, receptor activator of nuclear factor kappa-B ligand (RANKL), and osteoprotegerin (OPG) in the periodontal tissues, respectively, on the pressure side of the experimental tooth.ResultsIL-17 was detected in GCF and serum. The pressure area exhibited alveolar bone resorption only at a force of 20g. Additionally, a force of 60g led to root resorption. IL-17, RANKL/OPG and osteoclast number showed similar trend that all expressed increasing high level at early stage, then significantly decreased from days 5 to 14, and revealed 60g group the highest expression level while 0g group the lowest.The change in the IL-17 level in the GCF was strongly correlated with IL-17 and RANKL/OPG expression levels and osteoclast numbers in the periodontal ligament.ConclusionsThe results indicated that measuring IL-17 level in GCF can predict the risk of alveolar bone and root resorption induced by orthodontic treatment.

scholarly journals The past and present research at the University of Sydney’s Discipline of Orthodontics

2021 ◽  
Vol 11 ◽  
pp. 90-99
Author(s):  
Narayan H. Gandedkar ◽  
Oyku Dalci ◽  
M. Ali Darendeliler
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Light Emitting Diode ◽  
Orthodontic Tooth Movement ◽  
Stimulated Emission ◽  
Low Level Laser Therapy ◽  
Light Emitting ◽  
Level Laser ◽  
Light Amplification ◽  
The University

The University of Sydney’s Discipline of Orthodontics has been actively pursuing research in the areas of root resorption, sleep apnea, magnets in orthodontics, implants, acceleration of orthodontic tooth movement (OTM), and remote monitoring (RM). Our research has shed light on many specific factors that influence orthodontically induced inflammatory root resorption (OIIRR). We also explored the effects of some of the most discussed acceleration interventions on OTM and OIIRR, such as vibration, micro-osteoperforations, piezocision, low-level laser therapy, light emitting diode, light amplification by stimulated emission of radiation, and pharmacological substances. Further, we have researched into maximizing orthopedic treatment outcomes of maxillary deficient children with use of intraoral force application with utilization of miniscrew-assisted rapid palatal expander. We are currently trialing use of RM to facilitate orthodontic services in the public dental clinics.

scholarly journals Cementocyte cell death occurs in rat cellular cementum during orthodontic tooth movement

2016 ◽  
Vol 87 (3) ◽  
pp. 416-422 ◽  
Author(s):  
Humihiro Matsuzawa ◽  
Naoko Toriya ◽  
Yuya Nakao ◽  
Moe Konno-Nagasaka ◽  
Toshiya Arakawa ◽  
...  
Keyword(s):  
Cell Death ◽  
Caspase 3 ◽  
Tooth Movement ◽  
Root Resorption ◽  
Apoptotic Cell ◽  
Orthodontic Tooth Movement ◽  
Histochemical Staining ◽  
Tartrate Resistant Acid Phosphatase ◽  
Male Wistar Rats ◽  
The Right

ABSTRACT Objective: To clarify the mechanism of root resorption during orthodontic treatment, we examined cementocyte cell death and root resorption in the cellular cementum on the pressure side during experimental tooth movement. Materials and Methods: Using 8-week-old male Wistar rats, the right first molar was pushed mesiobuccally with a force of 40 g by a Ni-Ti alloy wire while the contralateral first molar was used as a control. Localization and number of cleaved caspase-3-positive and single-stranded DNA (ssDNA) - positive cells were evaluated using dual-label immunohistochemistry with anticleaved caspase-3 and anti-ssDNA antibodies. In addition, tartrate-resistant acid phosphatase (TRAP)-positive cells in the cellular cementum were evaluated using TRAP histochemical staining. Results: Caspase-3- and ssDNA-positive cells appeared at 12 hours, but were restricted to the compressed periodontal ligament (PDL) and not the cellular cementum. Cleaved caspase-3-positive cementocytes were observed in the cellular cementum adjacent to the compressed PDL on day 1. From days 2 to 4, the number of caspase-3- and ssDNA-positive cementocytes increased. TRAP-positive cells appeared on the cellular cementum at the periphery of the hyalinized tissue on day 7, and resorption progressed into the broad surface of the cementum by day 14. Conclusion: Cementocytes adjacent to the hyalinized tissue underwent apoptotic cell death during orthodontic tooth movement, which might have been associated with subsequent root resorption.

scholarly journals Injectable RANKL sustained release formulations to accelerate orthodontic tooth movement

2019 ◽  
Vol 42 (3) ◽  
pp. 317-325 ◽  
Author(s):  
Joy H Chang ◽  
Po-Jung Chen ◽  
Michael R Arul ◽  
Eliane H Dutra ◽  
Ravindra Nanda ◽  
...  
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Volume Fraction ◽  
Treatment Time ◽  
Orthodontic Tooth Movement ◽  
Hydroxyethyl Cellulose ◽  
Poly Lactic Acid ◽  
Tartrate Resistant Acid Phosphatase ◽  
Coil Spring ◽  
Control Groups

Summary Background Accelerating orthodontic tooth movement (OTM) through biologically effective methods, such as increasing osteoclast-mediated alveolar resorption, could effectively shorten treatment time. Objective To evaluate an injectable formulation containing receptor activator of nuclear factor kappa-B ligand (RANKL) on the OTM. Materials and methods We fabricated a RANKL formulation from 100 µl of 100 µg/ml RANKL adsorbed on 10 mg of poly(lactic acid-co-glycolic acid) microspheres embedded in a 10 wt% aqueous hydroxyethyl cellulose carrier gel. We characterized these formulations for the rate of RANKL release, and then tested for bioactivity using in vitro cell culture. In vivo OTM studies were conducted using 15 week old male Wistar rats for 14 days. We injected the RANKL formulations palatal to the left maxillary first molar and accomplished OTM with a nickel–titanium (NiTi) coil spring applying 5–8 g force. Control groups involved the application of NiTi coil spring with and without placebo formulation. The outcome measure included the distance of tooth movement, bone volume fraction, tissue density, and root volume determined with micro-computed tomography. We determined the amount of osteoclast activity using tartrate-resistant acid phosphatase (TRAP) staining. Results These formulations were able to sustain the release of RANKL for more than 30 days, and the released RANKL showed a positive effect on mice osteoclast precursor cells (RAW 264.7). Reported injectable RANKL formulations were effective in accelerating OTM compared with other control groups, with 129.2 per cent more tooth movement than no formulation and 71.8 per cent more than placebo formulation, corresponding with a significant increase in the amount of TRAP activity. We did not observe any significant differences in root resorption between the groups. Conclusion Our study shows a significant increase in OTM with injectable formulations containing RANKL.

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