scholarly journals The effect of alveolar decortication on orthodontically induced root resorption

2020 ◽  
Vol 90 (4) ◽  
pp. 524-531 ◽  
Author(s):  
Po-Jung Chen ◽  
Joy H. Chang ◽  
Eliane H. Dutra ◽  
Ahmad Ahmida ◽  
Ravindra Nanda ◽  
...  
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase ◽  
Micro Computed Tomography ◽  
Root Volume ◽  
Male Wistar Rats ◽  
Group 3 ◽  
Group 2

ABSTRACT Objective To determine the effect of alveolar decortication on orthodontically induced root resorption. Materials and Methods A total of 24 male Wistar rats (14 week old) were used. The rats were randomly divided into one of the following three groups: group 1 (control group), orthodontic tooth movement (OTM) for 2 weeks; group 2, OTM for 2 weeks + two alveolar decortications (2AD); group 3, OTM for 2 weeks + four alveolar decortications (4AD). The first molar was moved mesially for 2 weeks. Micro computed tomography was used to analyze root volume. In addition, histological sections were stained with Tartrate Resistant Acid Phosphatase (TRAP) to quantify the osteoclast number. Results The buccal root volume in OTM + 4AD group was decreased by 8.92% and 6.11% when compared with the OTM-only group and OTM + 2AD group, respectively. Similarly, the other four root volumes in the OTM + 4AD group was decreased by 8.99% and 5.24% when compared with the OTM-only group and OTM + 2AD group, respectively. There was a decrease in buccal root density in the OTM + 4AD group by 4.66% and 3.56% when compared with the OTM-only group and the OTM + 2AD group, respectively. In addition, there was an increase in the number of osteoclasts by 195.73% and 98.74% in OTM + 4AD group in comparison with the OTM and OTM + 2AD group. Conclusions The amount of orthodontically induced root resorption was positively correlated with the extent of surgical injury used to accelerate orthodontic tooth movement.

scholarly journals The effects of binge-pattern alcohol consumption on orthodontic tooth movement

2014 ◽  
Vol 19 (6) ◽  
pp. 93-98 ◽  
Author(s):  
Cristiano Miranda de Araujo ◽  
Aline Cristina Batista Rodrigues Johann ◽  
Elisa Souza Camargo ◽  
Orlando Motohiro Tanaka
Keyword(s):  
Bone Resorption ◽  
Saline Solution ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase ◽  
Chi Square ◽  
Significance Level ◽  
Male Wistar Rats ◽  
Chi Square Test

OBJECTIVE: This study aimed to assess tissue changes during orthodontic movement after binge-pattern ethanol 20% exposure.METHODS: Male Wistar rats (n = 54) were divided into two groups. The control group (CG) received 0.9% saline solution, while the experimental group (EG) received 20% ethanol in 0.9% saline solution (3 g/kg/day). On the 30th day, a force of 25 cN was applied with a nickel-titanium closed coil spring to move the maxillary right first molar mesially. The groups were further divided into three subgroups (2, 14 and 28 days). Tartrate-resistant acid phosphatase and picrosirius were used to assess bone resorption and neoformation, respectively. Data were compared by two-way ANOVA, Tukey's HSD, Games-Howell and chi-square test. Significance level was set at 5%.RESULTS: There was a decrease in the number of osteoclasts in EG at day 28. The percentage of collagen showed no interaction between group and time.CONCLUSION: Binge-pattern 20% ethanol promoted less bone resorption at the end of tooth movement, thereby suggesting delay in tooth movement.

scholarly journals Infrared Light-Emitting Diode (LED) Effects on Orthodontic Tooth Movement

2019 ◽  
Vol 30 (4) ◽  
pp. 410-416 ◽  
Author(s):  
Simone Peixe Friedrichsdorf ◽  
Victor Elias Arana-Chavez ◽  
Vivian Bradaschia-Correa ◽  
Paolo Maria Cattaneo ◽  
Gladys Cristina Dominguez
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Light Emitting Diode ◽  
Histological Study ◽  
Orthodontic Tooth Movement ◽  
Infrared Light ◽  
Tartrate Resistant Acid Phosphatase ◽  
Micro Computed Tomography ◽  
Led Phototherapy ◽  
Significant Difference

Abstract The present study aimed to analyze the effect of LED phototherapy on the presence of hyalinization and root resorption during orthodontic tooth movement (OTM) in rats and to measure the amount of tooth movement. Eighty rats were allocated into two groups: LED and control (CON), where the LED rats were irradiated with infrared LED (850 nm, 30 mW) for 5 min during the first five days of OTM and where controls were not irradiated. Both groups were subdivided into four subgroups (n=10) according to the date of euthanasia (4, 7, 14 and 21 days). Five out of ten LED21 and five of ten CON21 rats were submitted to micro-computed tomography (μCT); μCT scans were taken on days 0, 7, 14 and 21. For histological study, maxillae were processed to light microscopy using Hematoxylin-Eosin (HE) and Tartrate-Resistant Acid Phosphatase (TRAP) histochemistry. The amount of tooth movement did not differ between LED and CON. Hyalinization was observed at the pressure areas in both groups, and it did not show a statistically significant difference between the groups. Root resorption was also observed in both groups after 7 days and it did not represent any differences between the two groups. LED phototherapy was not able to increase the amount of OTM. Similar characteristics of hyalinization and root resorption were observed in both groups.

scholarly journals Cementocyte cell death occurs in rat cellular cementum during orthodontic tooth movement

2016 ◽  
Vol 87 (3) ◽  
pp. 416-422 ◽  
Author(s):  
Humihiro Matsuzawa ◽  
Naoko Toriya ◽  
Yuya Nakao ◽  
Moe Konno-Nagasaka ◽  
Toshiya Arakawa ◽  
...  
Keyword(s):  
Cell Death ◽  
Caspase 3 ◽  
Tooth Movement ◽  
Root Resorption ◽  
Apoptotic Cell ◽  
Orthodontic Tooth Movement ◽  
Histochemical Staining ◽  
Tartrate Resistant Acid Phosphatase ◽  
Male Wistar Rats ◽  
The Right

ABSTRACT Objective: To clarify the mechanism of root resorption during orthodontic treatment, we examined cementocyte cell death and root resorption in the cellular cementum on the pressure side during experimental tooth movement. Materials and Methods: Using 8-week-old male Wistar rats, the right first molar was pushed mesiobuccally with a force of 40 g by a Ni-Ti alloy wire while the contralateral first molar was used as a control. Localization and number of cleaved caspase-3-positive and single-stranded DNA (ssDNA) - positive cells were evaluated using dual-label immunohistochemistry with anticleaved caspase-3 and anti-ssDNA antibodies. In addition, tartrate-resistant acid phosphatase (TRAP)-positive cells in the cellular cementum were evaluated using TRAP histochemical staining. Results: Caspase-3- and ssDNA-positive cells appeared at 12 hours, but were restricted to the compressed periodontal ligament (PDL) and not the cellular cementum. Cleaved caspase-3-positive cementocytes were observed in the cellular cementum adjacent to the compressed PDL on day 1. From days 2 to 4, the number of caspase-3- and ssDNA-positive cementocytes increased. TRAP-positive cells appeared on the cellular cementum at the periphery of the hyalinized tissue on day 7, and resorption progressed into the broad surface of the cementum by day 14. Conclusion: Cementocytes adjacent to the hyalinized tissue underwent apoptotic cell death during orthodontic tooth movement, which might have been associated with subsequent root resorption.

scholarly journals Inhibitory Effects of 4-Hexylresorcinol on Root Resorption Induced by Orthodontic Tooth Movement

2020 ◽  
Vol 10 (18) ◽  
pp. 6313
Author(s):  
Jun-Kyu Jang ◽  
Dae-Won Kim ◽  
Seong-Gon Kim ◽  
Tae-Woo Kim
Keyword(s):  
Alkaline Phosphatase ◽  
Bone Turnover ◽  
Turnover Rate ◽  
Tooth Movement ◽  
Root Resorption ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Micro Computed Tomography ◽  
Expression Levels ◽  
Experimental Group

Root resorption during orthodontic tooth movement (OTM) is caused by an imbalance between the bone turnover rate and applied mechanical stress. The administration of 4-hexylresorcinol (4HR) increases the bone turnover rate and factors associated with bone formation. Thus, 4HR may show protective activity against root resorption during orthodontic tooth movement (OTM). A total of 40 rats (male: 20; female: 20) were included in this study, and the mandibular first molar was subjected to excessive orthodontic force. The experimental group (n = 20) received 12.8 mg/kg of 4HR every 2 weeks. The controls (n = 20) received a solvent without 4HR. Both groups had the same sex distribution. On Day 28 after the initiation of OTM, all the animals were sacrificed for micro-computed tomography analysis, Western blot analysis, and immunohistochemistry. The ratios of the root length and root volume to the total volume were significantly higher in the experimental group compared to those in the control group (p < 0.05). The expression levels of OPG, RANKL, alkaline phosphatase, and Runx2 in the experimental group according to Western blotting were significantly higher in the experimental group compared to those in the control group (p < 0.05). Their expression was mainly found in the periodontal ligament area. In conclusion, the administration of 4HR decreased the root resorption caused by OTM and increased the expression levels of OPG, RANKL, alkaline phosphatase, and Runx2.

scholarly journals Tissue response resulting from different force magnitudes combined with corticotomy in rats

2019 ◽  
Vol 89 (5) ◽  
pp. 797-803
Author(s):  
Kriangkrai Kraiwattanapong ◽  
Bancha Samruajbenjakun
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Three Dimensional ◽  
Tissue Response ◽  
Control Group ◽  
Histomorphometric Analysis ◽  
Root Volume ◽  
Male Wistar Rats ◽  
Light Force ◽  
Cervical Root

ABSTRACT Objectives: To evaluate the amount of hyalinization and root resorption after application of light and heavy forces during corticotomy-assisted tooth movement. Materials and Methods: Forty-eight male Wistar rats were used. Eight animals were the control group (day 0). The other 40 animals were randomly divided into two groups using the split-mouth technique: (1) corticotomy combined light force (CLF) and (2) corticotomy combined heavy force (CHF). Nickel-titanium closed coil springs that generated 10 g (light force) and 50 g (heavy force) were used for maxillary first molar movement. three-dimensional root volume was evaluated at 0, 14, and 28 days. Percent hyalinization was analyzed at 0, 3, 7, 14, 21, and 28 days by histomorphometric analysis. Results: The CHF group showed significantly lower cervical root volume than the CLF group at 28 days. Compared with day 0, the CHF group showed significantly less root volume in both distobuccal and distopalatal roots at 28 days. The hyalinization percentages in the CHF group were significantly higher than the CLF group at days 3 and 21. Conclusions: Heavy force combined with corticotomy produced more periodontal ligament hyalinization and root resorption than the light force combined with corticotomy.

scholarly journals The role of inhibition of osteocyte apoptosis in mediating orthodontic tooth movement and periodontal remodeling: a pilot study

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Michele Kaplan ◽  
Zana Kalajzic ◽  
Thomas Choi ◽  
Imad Maleeh ◽  
Christopher L. Ricupero ◽  
...  
Keyword(s):  
Bone Density ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Tartrate Resistant Acid Phosphatase ◽  
Osteocyte Apoptosis ◽  
Saline Administration ◽  
Group 2 ◽  
Group 1

Abstract Background Orthodontic tooth movement (OTM) has been shown to induce osteocyte apoptosis in alveolar bone shortly after force application. However, how osteocyte apoptosis affects orthodontic tooth movement is unknown. The goal of this study was to assess the effect of inhibition of osteocyte apoptosis on osteoclastogenesis, changes in the alveolar bone density, and the magnitude of OTM using a bisphosphonate analog (IG9402), a drug that affects osteocyte and osteoblast apoptosis but does not affect osteoclasts. Material and methods Two sets of experiments were performed. Experiment 1 was used to specifically evaluate the effect of IG9402 on osteocyte apoptosis in the alveolar bone during 24 h of OTM. For this experiment, twelve mice were divided into two groups: group 1, saline administration + OTM24-h (n=6), and group 2, IG9402 administration + OTM24-h (n=6). The contralateral unloaded sides served as the control. The goal of experiment 2 was to evaluate the role of osteocyte apoptosis on OTM magnitude and osteoclastogenesis 10 days after OTM. Twenty mice were divided into 4 groups: group 1, saline administration without OTM (n=5); group 2, IG9402 administration without OTM (n=5); group 3, saline + OTM10-day (n=6); and group 4, IG9402 + OTM10-day (n=4). For both experiments, tooth movement was achieved using Ultra Light (25g) Sentalloy Closed Coil Springs attached between the first maxillary molar and the central incisor. Linear measurements of tooth movement and alveolar bone density (BVF) were assessed by MicroCT analysis. Cell death (or apoptosis) was assessed by terminal dUTP nick-end labeling (TUNEL) assay, while osteoclast and macrophage formation were assessed by tartrate-resistant acid phosphatase (TRAP) staining and F4/80+ immunostaining. Results We found that IG9402 significantly blocked osteocyte apoptosis in alveolar bone (AB) at 24 h of OTM. At 10 days, IG9402 prevented OTM-induced loss of alveolar bone density and changed the morphology and quality of osteoclasts and macrophages, but did not significantly affect the amount of tooth movement. Conclusion Our study demonstrates that osteocyte apoptosis may play a significant role in osteoclast and macrophage formation during OTM, but does not seem to play a role in the magnitude of orthodontic tooth movement.

The Effect of Apis mellifera Propolis on RUNX-2 and ALP during Remodeling of Orthodontic Tooth movement

2021 ◽  
pp. 2363-2366
Author(s):  
Budi Handayani ◽  
Mieke Sylvia Margaretha A R ◽  
Rini Devijanti Ridwan ◽  
Mohammed Aljunaid
Keyword(s):  
Apis Mellifera ◽  
Immunohistochemical Staining ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Laboratory Research ◽  
Control Group ◽  
Propolis Extract ◽  
Treatment Groups ◽  
Experimental Laboratory ◽  
Group 2

Herbal medicine is interesting including treatments that use propolis. Propolis collected by Apis mellifera bees has a very useful composition whose active ingredients are flavonoids, CAPE and saponin. This research aims to analyze the effect of Apis mellifera propolis on RUNX-2 and ALP during the remodeling period caused by the orthodontic tooth movement in Cavia cobaya. It constitutes an experimental laboratory study and a random sampling method. The method used experimental laboratory research on 28 Cavia cobaya, which were divided into four groups consisting of two control groups and two treatment groups which have been given 3% and 5% propolis extract respectively. RUNX-2 and ALP expression were evaluated using immunohistochemical staining. Materials and Methods: Propolis is a resin substance produced by bees. Cavia cobaya with orthodontic tooth movement by separator rubber. The control group (Group 1) included clinically healthy Cavia cobaya (n=7), (Group 2) included Cavia cobaya with orthodontic tooth movement (n=7). A treatment group (Group P1) Cavia cobaya have been treated with orthodontic tooth movement and propolis 3%, (Group P2) Cavia cobaya have been treated with orthodontic tooth movement and propolis 5% and observed for 17 days. Samples of maxilla were taken from the subjects for analysis on day 17. RUNX-2 and ALP expression were evaluated using immunohistochemical staining. Results: showed that RUNX-2 and ALP expression were found to increase in the tension side. Conclusion: Propolis extract at 5% concentration had ability in bone remodeling by increasing RUNX-2 and ALP expression in the tension area during orthodontic tooth movement.

scholarly journals The Administration of 4-Hexylresorcinol Accelerates Orthodontic Tooth Movement and Increases the Expression Level of Bone Turnover Markers in Ovariectomized Rats

2020 ◽  
Vol 21 (4) ◽  
pp. 1526 ◽  
Author(s):  
Kwang-Hyo Choi ◽  
Dae-Won Kim ◽  
Suk Keun Lee ◽  
Seong-Gon Kim ◽  
Tae-Woo Kim
Keyword(s):  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Ovariectomized Rats ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase ◽  
Sprague Dawley ◽  
Osteogenic Markers ◽  
Surgical Methods ◽  
Elevated Expression ◽  
Turnover Markers

Surgical methods for accelerating orthodontic tooth movement are limited by possible damage to the tooth root and patient discomfort. 4-Hexylresorcinol (4HR) has been shown to increase bone remodeling and may potentially facilitate tooth movement. This study investigated the (1) effect of 4HR administration on osteoblast-like cells and (2) effect of 4HR administration on tooth movement in ovariectomized rats. Saos-2 cells were treated with either 4HR or solvent (control). Protein expression levels were investigated 2, 8, and 24 h after treatment. Thirty ovariectomized Sprague-Dawley rats were divided into two experimental groups (A and B) and one control group. After installation of an orthodontic tooth movement device, groups A and B received subcutaneous weekly injections of 4HR (1.28 and 128 mg/kg). Micro-computerized tomography and histological analyses were performed after 2 weeks of tooth movement. The application of 4HR elevated expression of osteogenic markers in Saos-2 cells. Movement of the first molars was significantly greater in rats administered 4HR. Furthermore, the expression of bone morphogenic protein-2, receptor activator of nuclear factor kappa-B ligand, osteocalcin, and tartrate-resistant acid phosphatase were increased after 4HR administration. 4HR application demonstrated increased expression of osteogenic markers in Saos-2 cells and accelerated orthodontic tooth movement in rats.

scholarly journals Effect of Different Doses of Synthetic Parathyroid Hormone (1-34) on Bone around Implants: a Preclinical Rat Model

2019 ◽  
Vol 30 (1) ◽  
pp. 43-46 ◽  
Author(s):  
Carlos Eduardo Secco Mafra ◽  
Marcelo Sirolli ◽  
Marília Cabral Cavalcanti ◽  
Rodrigo Basílio Albuquerque dos Santos ◽  
Cláudio Mendes Pannuti ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Titanium Implant ◽  
Titanium Implants ◽  
Osteogenic Potential ◽  
Control Group ◽  
Cortical Region ◽  
Male Wistar Rats ◽  
Bone To Implant Contact ◽  
Group 3 ◽  
Group 2

Abstract The aim of this study was to evaluate the effect of a lower dose of parathyroid hormone- PTH (1-34) on osteogenic potential of bone healing around titanium implants inserted into the tibia of rats. A blind parallel study was conducted in 45 adult male Wistar rats. Each rat received one titanium implant (4.5 x 2.2 mm) and was randomly assigned to receive subcutaneous injections, three times/week for 30 days, of the following treatments: group 1 - 40 µg/kg of PTH (1-34) (n=15); group 2 - 2 µg/kg of PTH (1-34) (n=15) and; group 3 - only the vehicle required for hormone dissolution (n=15). Thirty days after surgery, the animals were sacrificed and specimens containing the implant and the surrounding bone were removed and processed for non-decalcified sections. The sections were evaluated according to the following histometric parameters: proportion of mineralized tissue (PMT) adjacent to the implant threads (500 µm band); bone filling within the limits of the threads (BF) and; bone-to-implant contact (BIC). For the cortical region, both hormone dosages (groups 1 and 2) promoted better results, for all parameters, when compared to control group (p<0.05). Similar results were observed for the BF parameter in the cancellous region (p=0.0394). Therefore, systemic administration of PTH (1-34) stimulates bone formation around titanium implants, even at low doses.

scholarly journals Effect of supplementary zinc on orthodontic tooth movement in a rat model

2016 ◽  
Vol 21 (2) ◽  
pp. 45-50 ◽  
Author(s):  
Mohammad Sadegh Ahmad Akhoundi ◽  
Rezvaneh Ghazanfari ◽  
Shahroo Etemad-Moghadam ◽  
Mojgan Alaeddini ◽  
Azam Khorshidian ◽  
...  
Keyword(s):  
Rat Model ◽  
Tooth Movement ◽  
Root Resorption ◽  
Orthodontic Tooth Movement ◽  
Cumulative Effect ◽  
Study Groups ◽  
Model Material ◽  
Bone Homeostasis ◽  
Male Wistar Rats ◽  
Hematoxylin Eosin

ABSTRACT Introduction: Osteoclasts and osteoblasts are responsible for regulating bone homeostasis during which the trace element zinc has been shown to exert a cumulative effect on bone mass by stimulating osteoblastic bone formation and inhibiting osteoclastic bone resorption. Objective: The aim of the present study was to investigate the effects of zinc (Zn) on orthodontic tooth movement (OTM) in a rat model. Material and Methods: A total of 44 male Wistar rats were divided into four groups of 11 animals each and received 0, 1.5, 20 and 50 ppm Zn in distilled water for 60 days. In the last 21 days of the study, nickel-titanium closed coil springs were ligated between maxillary right incisors and first molars of all rats, and tooth movement was measured at the end of this period. Histological analysis of hematoxylin/eosin slides was performed to assess root resorption lacunae, osteoclast number and periodontal ligament (PDL) width. Results: Mean OTM was calculated as 51.8, 49.1, 35.5 and 45 µm in the 0, 1.5, 20 and 50 ppm zinc-receiving groups, respectively. There were no significant differences in neither OTM nor histological parameters among the study groups (p > 0.05). Conclusion: According to the results obtained in the current investigation, increase in supplementary zinc up to 50 ppm does not affect the rate of OTM neither bone and root resorption in rats.

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