scholarly journals Proline synthesis and physiological response of cassava genotypes under in vitro salinity

2019 ◽  
Vol 49 (6) ◽  
Author(s):  
Milena Nascimento Cardoso ◽  
Aparecida Gomes de Araújo ◽  
Leila Albuquerque Resende Oliveira ◽  
Bruno Trindade Cardoso ◽  
Ana Veruska Cruz da Silva Muniz ◽  
...  

ABSTRACT: The objective of this research was to evaluate the proline synthesis and physiological response of cassava genotypes which were micro propagated and induced to salinity stress in vitro. Micro cuttings of approximately 1.0cm long with a single bud of genotypes TBRS Tapioqueira, BRS Verdinha and Lagoão which were previously established in vitro were inoculated in a MS medium containing different concentrations of NaCl (0; 25; 50; 75; 100mM) and were analyzed after 90th day for: number of roots, number of leaves and shoot dry mass. The proline content of BRS Tapioqueira and Lagoão was assessed at 30th, 60th and 90th day. There was no analysis of proline of the variety Verdinha because of the contamination of the explants. The experimental design was completely randomized in double factorial scheme (3 genotypes x 5 salt treatments), with seven repetitions for growth variables. For comparing proline content, completely randomized design was used in a plot subdivided in time, with genotype and NaCl factors in plot and time in subplot, with two repetitions. For r time and genotypes Tukey test (P<0,05) was used and for NaCl levels regression test (P<0,05). Salinity affected the growth of all varieties; although, BRS Tapioqueira and BRS Verdinha were less affected by induced salt stress. There was an increase in the accumulation of proline from the salt increment, this synthesis of proline being a biochemical indicator of salt stress in cassava plants cultivated in vitro.

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 


2015 ◽  
Vol 6 (1) ◽  
pp. 20
Author(s):  
Erna Sinaga ◽  
Megayani Sri Rahayu ◽  
Awang Maharijaya

<p>ABSTRACT</p><p>The objectives of this study were to study the effect of several concentrations of polyethylene glycol (PEG) on the in vitro growth of eggplant, to find the appropriate PEG concentration for in vitro selection to drought  tolerance  of eggplant  and the drought tolerant eggplant accessions. The experiment  was conducted  at  the  Laboratory  of  Tissue  Culture,  Department  of  Agronomy and Horticulture,  Bogor  Agricultural  University.  The  experiment  was arranged  in  a  completely randomized design with two factor. The first factor was concentration of PEG (0, 5, 10,  and  15%) while the second factor was eggplant accessions (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  and  090).  The  results  showed  that  the addition  of PEG  to  in  vitro media significantly affected the survival percentage, the percentage of callus, developed the bud and the number of leaves of eggplant. Addition of PEG 10 and 15% in media can be used as the drought tolerance selective agent of eggplant in vitro. Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, and 090 were eggplant accessions which might be tolerant to drought.</p><p>Keywords: in vitro selection, solanaceae, tissue culture, tolerant, drought</p><p> </p><p>ABSTRAK</p><p>Penelitian ini bertujuan untuk  mempelajari pengaruh beberapa konsentrasi polietilena glikol (PEG)  terhadap  pertumbuhan  tanaman  terung  in  vitro, mendapatkan  konsentrasi  PEG  yang  dapat digunakan  untuk seleksi tanaman terung secara in vitro  dan nomor terung toleran terhadap cekamankekeringan. Penelitian ini dilaksanakan di laboratorium Kultur Jaringan,  Departemen Agronomi dan Hortikultura,  Institut  Pertanian  Bogor.  Penelitian  ini  disusun dalam  rancangan  acak  lengkap  dua faktor. Faktor pertama adalah konsentrasi PEG  terdiri atas  0, 5, 10, dan 15%.  Faktor kedua adalah nomor terung terdiri atas enam belas nomor (Kania F1, 001, 007, 013, 016, 030, 034, 035, 055, 057, 069,  071,  072,  078,  085,  dan  090).  Hasil  penelitian menunjukkan  bahwa  penambahan  PEG  pada media  in  vitro  memberikan pengaruh  nyata  dan  sangat  nyata  terhadap  persentase  hidup eksplan, persentase  eksplan  berkalus,  pertambahan  tinggi  tunas,  dan jumlah  daun  tanaman  terung.  Media PEG 10 dan 15% merupakan media yang dapat digunakan untuk seleksi kekeringan tanaman terung in vitro. Nomor terung Kania F1, 001, 007, 016, 034, 035, 055, 057, 069, 071, 072, 078, 085, dan 090 merupakan nomor-nomor terung yang toleran terhadap cekaman kekeringan.</p><p>Kata kunci: kultur jaringan, seleksi in vitro, solanaceae, toleran kekeringan</p>


Nativa ◽  
2018 ◽  
Vol 6 (6) ◽  
pp. 600
Author(s):  
Vanessa Cláudia Vasconcelos Segundo ◽  
Renato Innecco ◽  
Ebenézer De Oliveira Silva

O meloeiro tem grande importância econômica para o Nordeste brasileiro. Entretanto, em seu cultivo utiliza-se insumos que podem prejudicar o ambiente; como, os fertilizantes nitrogenados. Objetivou-se avaliar a emissão de N2O em cultivo de meloeiro amarelo ‘Goldex’. O experimento foi instalado em delineamento inteiramente casualizado, com cinco repetições. Os tratamentos foram: 0 (controle), 1,0; 1,5 e 2 vezes a quantidade de nitrogênio recomendada para cada estádio. Foi analisado o fluxo de N2O, área foliar, número de folhas, massa seca da parte aérea, massa seca dos frutos, massa seca da parte radicular, nitrogênio total do solo, das plantas e N (NH4+ e NO3-) do solo. O fluxo de N2O teve comportamento temporal irregular durante a fase de plântulas e crescente na floração. Os tratamentos afetaram significativamente a área foliar, número de folhas, massa seca da parte aérea, massa seca da parte radicular, nitrogênio da parte aérea e parte radicular das plantas e do solo. A aplicação de nitrogênio, em dosagens superiores à recomendada, não aumentou a produção do meloeiro. E o cálculo do fator de emissão mostrou que a emissão do N2O não atingiu valores prejudiciais ao ambiente.Palavras-chave: fator de emissão, efeito estufa e nitrogênio. NITROUS OXIDE EMISSION ASSOCIATED WITH UREA USE THE YELLOW MELON CROP ABSTRACT: The melon has great economic importance for the Brazilian Northeast. However, in its cultivation uses inputs that can harm the environment; Such as nitrogen fertilizers. The objective of this study was to evaluate the emission of N2O in yellow 'Goldex' cultivar. The experiment was installed in a completely randomized design with five replications. The treatments were: 0 (control), 1.0; 1.5 and 2 times the amount of nitrogen recommended for each stage. N2O flux, leaf area, leaf number, shoot dry mass, fruit dry mass, dry mass of the root portion, total soil nitrogen, of the plants and N (NH4 + and NO3 -) of the soil were analyzed. The flow of N2O had irregular temporal behavior during the seedling phase and increased in flowering. The treatments affected significantly the leaf area, number of leaves, dry mass of the aerial part, dry mass of the root part, nitrogen of the aerial part and root part of the plants and the soil. The application of nitrogen, in dosages higher than the recommended one, did not increase the production of the melon. And the calculation of the emission factor showed that the emission of N2O did not reach values harmful to the environment.Keywords: emission fator, greenhouse and nitrogen.


2015 ◽  
Vol 46 (4) ◽  
pp. 590-592 ◽  
Author(s):  
Cibele Mantovani ◽  
Kathia Fernandes Lopes Pivetta

ABSTRACT: The objective of this paper was to evaluate the effects of different honey concentrations in culture media, in comparison to sucrose medium, for the in vitro development of the epiphytic Encyclea cordigera orchid, in order to improve the process of propagation of the species. The in vitro germination was prepared on a reduced Murashige & Skoog (MS) medium. After 90 days, the seedlings were divided into different treatments, where they remained for another 90 days. Six treatments were set up (30g L-1 of sucrose; 15, 30, 45, and 60g L-1 of honey; and absence of any carbohydrates) in a completely randomized design. Plants were removed from the vials 270 days after the start of the experiment, and the number of roots, length of the largest leaf, length of the longest root, number of leaves, and fresh and dry masses were evaluated. Data concerning the number of leaves and roots were (x+1)1/2 transformed and subjected to an analysis of variance (ANOVA); the means were compared by a Tukey's test set at 5% probability. Medium containing 60g L-1 of honey proved to be superior to the sucrose medium traditionally used, favoring the in vitro growth and development of Encyclea cordigera. This medium can therefore be recommended for the propagation of this species, which is usually cultivated as an ornamental plant.


Agriculture ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 456
Author(s):  
Bruno Fróes Campos ◽  
Anselmo Junior Corrêa Araújo ◽  
Cristina Aledi Felsemburgh ◽  
Thiago Almeida Vieira ◽  
Denise Castro Lustosa

The application of Trichoderma fungus can be used to obtain seedlings of higher quality for plantations. The objective of this study was to evaluate the effects of Trichoderma isolates on the germination and initial growth of açaí seedlings. Two trials were set up. In the laboratory, the seeds were inoculated in suspensions prepared with each of the isolates for 24 h, and they were arranged in trays containing filter paper in a completely randomized design with five replicates containing 50 seeds each. For the control, the seeds were submerged only in water. Part of the seedlings resulting from seeds treated and not treated with Trichoderma were transferred to bags containing forest soil and were submitted to seven modes of application of Trichoderma and kept in a nursery. The speed of germination and hypocotyl were not influenced by the biological treatment. The application of the different Trichoderma isolates positively influenced the height, collar diameter, number of leaves, root size, leaf area and root, and aerial part dry mass of the açaí seedlings. The application of the Trichoderma isolate TAM01 on the preplanting substrate and biweekly applications on the postplanting substrate was the combination that most positively influenced the development of açai seedlings.


2019 ◽  
Vol 5 (2) ◽  
pp. 67
Author(s):  
Mardiana Mardiana ◽  
Zainuddin Zainuddin ◽  
Mahfudz Mahfudz ◽  
Hawalina Hawalina

Kiwi fruit takes about 25 weeks from flower bloom until it reaches physiological maturity, so the time required to produce kiwi seeds from seeds in large quantities and uniform is very long. Tissue culture is one method that can be used to obtain a lot of kiwi seeds and uniforms with large quantities in a faster time. The purpose of this study was to examine various types of media compaction materials for the growth of kiwi shoots in vitro. This study was prepared based on Completely Randomized Design (RAL) with 5 treatments and repeated 4 times so that there were 20 experimental units, each experiment using 2 explants so that there are 40 eksplan. The treatments were: MA 1: Agar Swallow Globe 8 g / l, MA 2: Agar Swallow Globe 4 g / l + Agar Nutrijell 4 g / l, MA 3: Agar Swallow Globe 4 g / l + Agar Nutrijell 5 g / l, MA 4: Phytagel 2.2 g / l, MA 5: Agar Nutrijell 11 g / l. Observation variables are When shoots appear, Number of shoots, number of leaves, Number of Roots, number of root hair. The results showed Swallow Globe 4 g / l + Agar Nutrijell 4 g / l treatment gave the highest average number of shoots, the highest number of leaves and roots, this proved that the combination of Swallow Globe and Nutrijell agar gave a good condition for shoot growth kiwi plant.t.


Author(s):  
Subandi M ◽  
Arkhan Jannata ◽  
Sofiya Hasani

The propagation of Cavendish (Musa acuminata L.) seedling conventionally relatively took a longer time, not uniformed in quality and possibly has a disease that is derived from unhealthy mother stock. This research aimed to find the effect of IBA and BA on difference concentrations on the growth of plantlet, also to determine the optimum concentration of IBA and BA on the growth of Cavendish bud plantlets in vitro. This research had been carried out started from April to July 2018 in Tissue Culture Laboratory, Research Institute of Horticultural Seed Development of Pasir Banteng, Sumedang, West Java. Indonesia. The method used in this research was Completely Randomized Design with two factors. The first factor was IBA (i0 = 0 mg L-1, i1= 0,75 mg L-1, dan i2= 1,5 mg L1), and the second factor was BA (b1= 1 mg L-1 b2= 3 mg L-1 b3= 5 mg L-1 b4= 7 mg L-1), replicated three times. Duncan Multiple Range Test (DMRT) was used as a further test. The result showed an interaction between IBA and BA on the parameters of shoot growth and the number of leaves at 6 WAC. IBA showed an independent effect on the number of buds. The addition of 1,5 mg L-1 IBA and 3 mg L-1 BA was optimum for the growth of Cavendish bud explants.


2020 ◽  
Vol 44 ◽  
Author(s):  
Hugo Cesar Rodrigues Moreira Catão ◽  
Franciele Caixeta ◽  
Amanda Moreira Lopes ◽  
Flavia Andrea Nery-Silva ◽  
Adílio de Sá Júnior

ABSTRACT Salinity influences all germination stages and may adversely affect seedling establishment in the field. The objective of this research was to determine the relationship between the antioxidant activity and the physiological performance of seeds and to verify the efficacy of the imaging analysis of popcorn seedlings after seed exposure to saline stress. A completely randomized design was used that consisted of four replicates in a factorial scheme. This scheme comprised three popcorn hybrids (P618, AP6002, and AP8203) and five saline potential levels (0.0; -0.1; -0.3; -0.6, and -0.9 MPa) that were obtained from KCl solutions of different concentrations. First count germination, germination and seedling vigor classification (strong normal seedlings), and dry mass of seedlings were evaluated to determine the physiological quality of the seed. The images of seedlings were used to evaluate the coleoptile and root lengths, indices of vigor, uniformity, and growth using the Groundeye® software. The activities of the antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) were also determined. Saline stress compromises the physiological quality and antioxidant activity of seeds and the performance of popcorn seedlings. Computerized image analysis using Groundeye® was efficient in evaluating the popcorn seedlings after being subjected to salt stress. Hybrids AP6002 and AP8203 were more tolerant to salt stress than hybrid P618.


Kultivasi ◽  
2020 ◽  
Vol 19 (3) ◽  
Author(s):  
Ega Raisya ◽  
Denny Sobardini Sobarna ◽  
Anne Nuraini ◽  
Syariful Mubarok ◽  
Erni Suminar ◽  
...  

Sari Perbanyakan tanaman stroberi secara konvensional dilakukan dengan menggunakan stolon, tetapi kurang efektif serta kualitas bibit yang dihasilkan kurang baik akibat adanya akumulasi penyakit. Budidaya stroberi memerlukan adanya perbanyakan bibit secara massal, tetapi tidak mengubah kualitasnya. Multiplikasi in vitro menjadi solusi untuk penyediaan bibit berkualitas dalam jumlah besar. Upaya untuk mendapatkan tunas in vitro dalam jumlah banyak yakni perlu adanya penambahan zat pengatur tumbuh golongan sitokinin seperti Benzylaminopurine (BAP) atau Thidiazuron (TDZ). Tujuan penelitian ini adalah mengetahui dan menetapkan jenis serta konsentrasi sitokinin dengan hasil terbaik dalam multiplikasi stroberi kultivar Tochiotome. Percobaan dilaksanakan di Laboratorium Kultur Jaringan Tanaman, Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran. Penelitian menggunakan Rancangan Acak Lengkap yang terdiri dari tujuh perlakuan yang diulang lima kali, yaitu: Kontrol (tanpa sitokinin); BAP (0,25 ppm; 0,50 ppm; 0,75 ppm), dan TDZ (0,25 ppm; 0,50 ppm; 0,75 ppm). Hasil dari percobaan menunjukkan bahwa penambahan sitokinin tidak berpengaruh nyata terhadap jumlah tunas dan bobot segar planlet. Media perlakuan kontrol dapat menghasilkan jumlah akar lebih banyak dibandingkan dengan media ditambah sitokinin. Penambahan BAP 0,50 ppm  berpengaruh positif terhadap jumlah daun dan dapat menghasilkan runner secara in vitro. Pemberian BAP 0,50 ppm cenderung dapat meningkatkan dan mempercepat produksi bibit tanaman stroberi kultivar Tochiotome.Kata Kunci: Benzylaminopurine (BAP), Thidiazuron (TDZ), Stroberi, Kultur Jaringan AbstractStolon is used for conventional propagation of strawberry, but it is less effective and the quality of the seeds is not good due to the accumulation of disease. In vitro multiplication becomes a solution for the supply of quality seeds in a fast time. The addition of growth regulator cytokinin, such as Benzylaminopurine (BAP) or Thidiazuron (TDZ) can produced the large number of shoot. The objective of this study was to obtain the best type and concentration of cytokinin in the multiplication of strawberry ‘Tochiotome’. The study was conducted at the Plant Tissue Culture Laboratory, Seed Technology, Faculty of Agriculture, Universitas Padjadjaran. This study used a Completely Randomized Design (CRD) with seven treatments and five replications, that were: Control (without cytokinin); BAP (0.25 ppm; 0.50 ppm; 0.75 ppm), and TDZ (0.25 ppm; 0.50 ppm; 0.75 ppm). The results indicated that addition of cytokinin did not affected increasing number of shoots and fresh weightof plantlets. Control media can produce larger number of roots than those containing PGRs, this might be due to the endogenous auxin concentrations found in strawberry plants. Also, cytokinin inhibited root formations process. Plants treated with BAP 0.50 ppm increased for the number of leaves and produced runners in vitro. This study showed application of BAP with 0.50 ppm increased and accelerated the production of strawberry ‘Tochiotome’ seedlings.Keywords: Benzylaminopurine (BAP), Thidiazuron (TDZ), Strawberry, Tissue Culture


2018 ◽  
Vol 9 (2) ◽  
pp. 142-153
Author(s):  
Edna Maria Bonfim-Silva ◽  
Juliana Tertezinha Sasso Paludo ◽  
Tonny José Araújo da Silva ◽  
William Fenner ◽  
Maurício Dutra Zanotto

It is essential to have knowledge of changes in soil structure, as a result of agricultural use and its relationship with crop development, especially those with potential for use in new regions, such as the cultivation of safflower (Carthamus tinctorius L.) in the Cerrado. In view of this dynamics, the objective was to evaluate the phytometric components of safflower genotypes submitted to levels of soil densities in Cerrado Oxisol. Ten safflower genotypes were then submitted to compaction (five densities: 1.0, 1, 2, 1.4, and 1.8 Mg m-3) in a completely randomized design with four replications under greenhouse conditions. The plots consisted of an overlap of three rings of polyvinylidene chloride of 0.01 m height each, and the treatments were applied in the intermediate layer. The phytometric components (plant height, number of leaves and stem diameter) and shoot dry mass of the crop were evaluated at 30, 60 and 90 days after sowing. There was no significant interaction between safflower genotypes and soil densities, with the effects being isolated for genotypes and/or densities when significant. For the phytometric characteristics evaluated, the genotypes PI250196, PI301049, PI305173, and PI305205 are more stable about the variations of soil density in comparison to the others. The phytometric components and dry mass of safflower shoot are impaired from the mean density of 1.2 Mg m-3.


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