Evaluation of hollocelulase production by Lentinula edodes (Berk.) Pegler during the submerged fermentation growth using RSM

The cellulase proteins have a great importance in the enzymatic hydrolysis of woody biomass. Despite of costs being a major concern, it has been a stimulus to study basidiomycetes biochemical properties which degrade lignocellulosic material and have prompted the processes' study for obtaining cellulolytic enzymes in fungi. The objective of this research was to evaluate the effects of the initial nitrogen content on (ammonium sulfate) and on sugar cane bagasse, which hereby, acts as an inducer of hydrolytic enzymes to produce cellulases and xylanases, using three Lentinula edodes (Berk.) Pegler strains as a transformation agent. A factorial design with 22 replications in the central point was conducted, varying concentrations of ammonium sulfate and sugar cane bagasse. The submerged cultures carried out in synthetic culture medium and incubated at 25°C for 7 days on an orbital shaker at 150 rpm. The total protein and cellulase activity as endoglucanase, exoglucanase and β-glucosidase and the xylanase was also determined. The results showed that the production of hydrolytic enzymes was stimulated by the presence of high concentrations of sugar cane bagasse (30g/L), characterizing it as an inducer due to the demonstrated proportional relationship. Thus, ammonium sulfate acted as a reducing agent in the synthesis of enzymes, being the low concentrations (0.1g/L) indicated for the enzyme production system under study. Among the studied strains, the EF52 showed higher activity for xylanase, endoglucanases, β-glucosidase and also protein.

Download Full-text

Carbon nutrition and hydrolytic and cellulolytic activities in the ectomycorrhizal fungus Pisolithus tinctorius

Canadian Journal of Microbiology ◽

10.1139/m93-075 ◽

1993 ◽

Vol 39 (5) ◽

pp. 529-535 ◽

Cited By ~ 26

Author(s):

Weiguo Cao ◽

Don L. Crawford

Keyword(s):

Hydrolytic Enzymes ◽

Ectomycorrhizal Fungus ◽

Isozyme Pattern ◽

Pisolithus Tinctorius ◽

Cellulolytic Enzymes ◽

Cellulolytic Activity ◽

Gradient Gel Electrophoresis ◽

Carbon Nutrition ◽

High Concentrations ◽

Increased Activity

Four strains of an ectomycorrhizal fungus, Pisolithus tinctorius, were investigated for carbon nutrition, and for production of hydrolytic and cellulolytic enzymes. Glucose, mannose, and cellobiose supported rapid mycelial growth of all four strains. Fructose was utilized by two strains, SMF and S359. Of the 10 hydrolytic enzymes examined, acid phosphatase, acid α-galactosidase, acid esterase, and acid β-glucosidase were found in all four strains. β-Galactosidase was only observed in strain S359. α-Mannosidase, β-mannosidase, α-glucosidase, β-xylosidase, and proteinase were not detected in any of the four strains. Isozyme patterns of β-glucosidase and esterase in the four strains were compared by activity staining after native gradient gel electrophoresis. The isozyme pattern of β-glucosidase showed three major forms in all four strains. In addition, two more isoforms were found in strain S370. All strains shared two esterase bands, while strain S370 had three more isoforms. Study on strain SMF indicated that acid β-glucosidase was expressed constitutively, with increased activity in cellobiose-containing media. Under nitrogen-limiting conditions, a low level of endoglucanase and exoglucanase activity was observed in strains SMF and S359. Further study on S359 showed that high concentrations of nitrogen repressed the cellulolytic activity. When cellobiose served as carbon source, higher cellulolytic activity was observed. Cellulose did not induce higher activity.Key words: Pisolithus, ectomycorrhizal, β-glucosidase, hydrolytic enzymes, cellulolytic enzymes.

Download Full-text

Production of cellulase from Aspergillus niger and Trichoderma reesei mixed culture in carboxymethylcellulose medium as sole carbon

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d201211 ◽

2019 ◽

Vol 20 (12) ◽

Author(s):

Dia Septiani ◽

HERMAN SURYADI ◽

Abdul Mun'im ◽

WIBOWO MANGUNWARDOYO

Keyword(s):

Aspergillus Niger ◽

Ammonium Sulfate ◽

Mixed Culture ◽

Trichoderma Reesei ◽

Crude Extract ◽

Hydrolytic Enzymes ◽

Cellulase Activity ◽

Cellulase Production ◽

Partial Purification ◽

Cellulose Depolymerization

Abstract. Septiani D, Suryadi H, Mun’im A, Mangunwardoyo W. 2019. Production of cellulase from Aspergillus niger and Trichoderma reesei mixed culture in carboxymethylcellulose medium as sole carbon. Biodiversitas 20: 3539-3544. Cellulase is one of hydrolytic enzymes that breakdown cellulose into glucose. Cellulases are promising to be applied in natural products which may improve the yield of bioactive in plant extract through cellulose depolymerization. Cellulases from mixed culture of Aspergillus niger and Trichoderma reesei can produce a high cellulase activity because of the synergism activity among endoglucanase, exoglucanase, and also β-glucoside. Cellulase production and partial purification of monoculture and mixed culture (1:1) of these fungi on carboxymethylcellulose media were investigated in this study. Total cellulase activity was measured by filter paper assay followed by protein estimation with Bradford method. The crude extract of Aspergillus niger monoculture has the highest cellulase activity (0.131 U/mL, P<005) followed by mixed culture (0.109 U/mL) and Trichoderma reesei (0.106 U/mL). The cellulase activity of partially purified cellulase from mixed culture significantly increased (0.335, 0.348, 0.374 U/mL, P<0.05) compared to crude extract along with stepwise addition of ammonium sulfate. Cellulase activity of mixed culture at 80% ammonium sulfate increase up to 2.238-fold and showed highest value (P<0.05) compared to monocultures. In conclusion, combination of Aspergillus niger and Trichoderma reesei fungi in carboxymethyl cellulose media followed by 80% ammonium sulfate precipitation can be a promising cellulase production with high cellulase activity.

Download Full-text

HIGH YIELD OF ETHANOL FROM WASTE APPLE JUICE by IMMOBILIZED CELLS OF Saccharomyces cerevisiae S-3S ON SUGAR CANE BAGASSE IN FED BATCH SYSTEM

Environmental Engineering and Management Journal ◽

10.30638/eemj.2017.204 ◽

2017 ◽

Vol 16 (9) ◽

pp. 1867-1871

Author(s):

Arifa Tahir ◽

Sidra Sarwar

Keyword(s):

Saccharomyces Cerevisiae ◽

Sugar Cane ◽

Apple Juice ◽

Immobilized Cells ◽

Sugar Cane Bagasse ◽

High Yield ◽

Fed Batch ◽

Batch System

Download Full-text

The Sustainable Production of a Novel Laccase from Wheat Bran by Bordetella sp. JWO16: Toward a Total Environment

Catalysts ◽

10.3390/catal11060677 ◽

2021 ◽

Vol 11 (6) ◽

pp. 677

Author(s):

John Onolame Unuofin

Keyword(s):

Wheat Bran ◽

Contaminated Soils ◽

Residual Activity ◽

Dye Decolorization ◽

Fermentation Medium ◽

Biochemical Properties ◽

Synthetic Dyes ◽

High Concentrations ◽

Capital Intensive ◽

Agroindustrial Residue

Laccase is increasingly adopted in diverse industrial and environmental applications, due to its readily accessible requirements for efficient catalytic synthesis and biotransformation of chemicals. However, it is perceived that its industrial production might incur some unfavorable overhead, which leads to expensive market products, and the corresponding negative environmental feedback, due to the use of capital-intensive and precarious chemicals. To this end, this study was designed to evaluate the performance indicators of the valorization of wheat bran by a novel Jb1b laccase and its subsequent application in waste minimization and water management, on a laboratory scale. Optimal Jb1b laccase was produced in submerged fermentation medium containing wheat bran, an agroindustrial residue, through response surface methodology (RSM) algorithm, and was applied in dye decolorization and denim bioscouring, respectively. Results showed that the resultant enzyme manifested unique biochemical properties, such as enhanced tolerance at certain physicochemical conditions, with a residual activity of at least ca. 76%. Furthermore, phenomenally high concentrations of synthetic dyes (0.2% w v−1) were decolorized over 56 h, and a 6 h mediator-supported simultaneous denim bleaching and decolorization of wash effluent was observed. The sustainability of the production and application processes were inferred from the reusability of the fermentation sludge as a potential biofertilizer, with subsequent prospects for the biostimulation and bioaugmentation of contaminated soils, whereas the decolorized water could be adopted for other uses, amongst which horticulture and forestry are typical examples. These phenomena therefore authenticate the favorable environmental feedbacks and overhead realized in this present study.

Download Full-text

Electrochemical Corrosion of Galvanized Steel in Binary Sustainable Concrete Made with Sugar Cane Bagasse Ash (SCBA) and Silica Fume (SF) Exposed to Sulfates

Applied Sciences ◽

10.3390/app11052133 ◽

2021 ◽

Vol 11 (5) ◽

pp. 2133

Author(s):

Laura Landa-Ruiz ◽

Miguel Angel Baltazar-Zamora ◽

Juan Bosch ◽

Jacob Ress ◽

Griselda Santiago-Hurtado ◽

...

Keyword(s):

Silica Fume ◽

Sugar Cane ◽

Electrochemical Techniques ◽

Aggressive Medium ◽

Sugar Cane Bagasse ◽

Galvanized Steel ◽

Engineering Structures ◽

Conventional Concrete ◽

Sustainable Concrete ◽

Sugar Cane Bagasse Ash

This research evaluates the behavior corrosion of galvanized steel (GS) and AISI 1018 carbon steel (CS) embedded in conventional concrete (CC) made with 100% CPC 30R and two binary sustainable concretes (BSC1 and BSC2) made with sugar cane bagasse ash (SCBA) and silica fume (SF), respectively, after 300 days of exposure to 3.5 wt.% MgSO4 solution as aggressive medium. Electrochemical techniques were applied to monitor corrosion potential (Ecorr) according to ASTM C-876-15 and linear polarization resistance (LPR) according to ASTM G59 for determining corrosion current density (icorr). Ecorr and icorr results indicate after more than 300 days of exposure to the sulfate environment (3.5 wt.% MgSO4 solution), that the CS specimens embedded in BSC1 and BSC2 presented greater protection against corrosion in 3.5 wt.% MgSO4 than the specimens embedded in CC. It was also shown that this protection against sulfates is significantly increased when using GS reinforcements. The results indicate a higher resistance to corrosion by exposure to 3.5 wt.% magnesium sulfate two times greater for BSC1 and BSC2 specimens reinforced with GS than the specimens embedding CS. In summary, the combination of binary sustainable concrete with galvanized steel improves durability and lifetime in service, in addition to reducing the environmental impact of the civil engineering structures.

Download Full-text