Assessment of zinc solubilization potential of zinc-resistant Pseudomonas oleovorans strain ZSB13 isolated from contaminated soil

2023 ◽  
Vol 83 ◽  
Author(s):  
H. F. Rehman ◽  
A. Ashraf ◽  
S. Muzammil ◽  
M. H. Siddique ◽  
T. Ali
Keyword(s):  
Contaminated Soil ◽  
Bacterial Strain ◽  
Bacterial Species ◽  
Resistant Bacteria ◽  
Zn Deficiency ◽  
Rrna Gene ◽  
Growth Enhancement ◽  
Pseudomonas Oleovorans ◽  
Human Beings ◽  
Bacterial Strains

Abstract Zinc is an essential micronutrient that is required for optimum plant growth. It is present in soil in insoluble forms. Bacterial solubilization of soil unavailable form of Zn into available form, is an emerging approach to alleviate the Zn deficiency for plants and human beings. Zinc solubilizing bacteria (ZSB) could be a substitute for chemical Zn fertilizer. The present study aimed to isolate and characterize bacterial species from the contaminated soil and evaluate their Zn solubilizing potential. Zn resistant bacteria were isolated and evaluated for their MIC against Zn. Among the 13 isolated bacterial strains ZSB13 showed maximum MIC value upto 30mM/L. The bacterial strain with the highest resistance against Zn was selected for further analysis. Molecular characterization of ZSB13 was performed by 16S rRNA gene amplification which confirmed it as Pseudomonas oleovorans. Zn solubilization was determined through plate assay and broth medium. Four insoluble salts (zinc oxide (ZnO), zinc carbonate (ZnCO3), zinc sulphite (ZnS) and zinc phosphate (Zn3(PO4)2) were used for solubilization assay. Our results shows 11 mm clear halo zone on agar plates amended with ZnO. Likewise, ZSB13 showed significant release of Zn in broth amended with ZnCO3 (17 and 16.8 ppm) and ZnO (18.2 ppm). Furthermore, Zn resistance genes czcD was also enriched in ZSB13. In our study, bacterial strain comprising Zn solubilization potential has been isolated that could be further used for the growth enhancement of crops.

scholarly journals Prevalence of Diversified Antibiotic Resistant Bacteria within Sanitation Related Facilities of Human Populated Workplaces in Abbottabad

2020 ◽  
Author(s):  
Jawad Ali ◽  
Malik Owais Ullah Awan ◽  
Gulcin Akca ◽  
Iftikhar Zeb ◽  
Bilal AZ Amin ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Bacterial Resistance ◽  
Bacterial Species ◽  
Resistant Bacteria ◽  
Rrna Gene ◽  
Beta Lactam ◽  
Bacterial Strains ◽  
Antibiotic Resistant Bacteria ◽  
Sensitivity Testing ◽  
Antibiotic Resistant

AbstractAntibiotics discovery was a significant breakthrough in the field of therapeutic medicines, but the over (mis)use of such antibiotics (n parallel) caused the increasing number of resistant bacterial species at an ever-higher rate. This study was thus devised to assess the multi-drug resistant bacteria present in sanitation-related facilities in human workplaces. In this regard, samples were collected from different gender, location, and source-based facilities, and subsequent antibiotic sensitivity testing was performed on isolated bacterial strains. Four classes of the most commonly used antibiotics i.e., β-lactam, Aminoglycosides, Macrolides, and Sulphonamides, were evaluated against the isolated bacteria.The antibiotic resistance profile of different (70) bacterial strains showed that the antibiotic resistance-based clusters also followed the grouping based on their isolation sources, mainly the gender. Twenty-three bacterial strains were further selected for their 16s rRNA gene based molecular identification and for phylogenetic analysis to evaluate the taxonomic evolution of antibiotic resistant bacteria. Moreover, the bacterial resistance to Sulphonamides and beta lactam was observed to be the most and to Aminoglycosides and macrolides as the least. Plasmid curing was also performed for MDR bacterial strains, which significantly abolished the resistance potential of bacterial strains for different antibiotics. These curing results suggested that the antibiotic resistance determinants in these purified bacterial strains are present on respective plasmids. Altogether, the data suggested that the human workplaces are the hotspot for the prevalence of MDR bacteria and thus may serve the source of horizontal gene transfer and further transmission to other environments.

scholarly journals Bio-perceptions of Hydro carbon contaminated soil and its Bioremediation effect with Biological Consortia”

2020 ◽  
Vol 1 (3) ◽  
pp. 15-24
Author(s):  
Aswathy Chandran ◽  
Dr.S.Sujatha Jeyapaul
Keyword(s):  
Contaminated Soil ◽  
Bacterial Strain ◽  
Contaminated Soils ◽  
Bacterial Species ◽  
Research Work ◽  
Antagonistic Activity ◽  
Physical Parameters ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Experimental Soil

     The present research work has clearly denoted as initially estimation of physic-chemical properties of the experimental hydrocarbon contaminated soil. The texture of the soil plays a very important role in microbial and plant species establishment and development and also influences physical parameters of the soil. The current results are clearly showed experimental soil of the hydrocarbon contaminated soil possessed totally eight different autochthonus bacterial strains were provably identified viz., Acinetobacter, Mycobacterium sp., Bacillus sp., Pseudomonas sp., and Aeromonas sp., observed by Bergy’s Manual. When this experimental soil was remediated with two biological sources such as four allothonus bacterial strains named as Enterobacter sp., Flavobacter sp.,  Shigella sp., and Bacillus sp., along with agronomic wastes also addition with neem juice. From the present result showed that Enterobacter sp., subjected polluted soil was remediated maximum than other treated sources assessed by spectrometric data. While, the biofilm formation experiment also been definitely expressed biodegradation potential enriched allothonus bacterial strain was the following order Enterobacter sp., Flavobacter sp.,  Shigella sp., and Bacillus sp.,. Moreover, other interesting finding also had been profounded such as dominant Antagonistic activity potential possessed autochthonus bacterial strain from the hydrocarbon contaminated soil. It has been identified through the molecular identification those typical organism expressed the named as ‘’Pseudomonas aeruginosa PA96’’by 16sr RNA sequence analysis. Additionaly maximum and maximum antagonistic activity has been noticed on E.coli, more or less similar zone of inhibition showed on other bacterial species of Shijella sp., and K. pneumonia.  Moreover, HPLC results were almost elucidated fractions of hydrocarbon compounds thoroughly replied total illustrated chemical compounds are gradually minimized, when the heavy contaminated soils subjected with other bacterial sources along with various agronomic wastes.  It has been significantly reduced the spectrum of the total hydrocarbon derivatives when it compared with before treatment of the contaminated soils. Therefore, these allothonous bacterial organism Enterobacter sp., strains could be considered for future use for bioremediation of oil contaminated land. However, further studies are needed to evaluate the potential of the isolated strains to degrade hydrocarbons in situ, in natural environmental conditions. This could be equally applicable for any allothonously present or other bacterial strains ubiquitously available in nature, and the technology could be further developed for targeting of any pollutants present on earth creating enormous environmental and health hazards.

scholarly journals Potential risks of antibiotic resistant bacteria and genes in bioremediation of petroleum hydrocarbon contaminated soils

2020 ◽  
Vol 22 (5) ◽  
pp. 1110-1124 ◽  
Author(s):  
Colin J. Cunningham ◽  
Maria S. Kuyukina ◽  
Irena B. Ivshina ◽  
Alexandr I. Konev ◽  
Tatyana A. Peshkur ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Petroleum Hydrocarbon ◽  
Resistant Bacteria ◽  
Careful Selection ◽  
Bacterial Strains ◽  
Antibiotic Resistant ◽  
Potential Risks ◽  
Selection Of

The problems associated with potential risks of antibiotic resistance spreading during bioremediation of oil-contaminated soil are discussed. Careful selection of bacterial strains and pretreatment of organic wastes used as fertilizers are suggested.

Isolation and Genetic Identification of Dibenzothiophene Degrading Bacteria from Contaminated Soil

2012 ◽  
Vol 610-613 ◽  
pp. 292-295 ◽  
Author(s):  
Lin Li ◽  
Chao Cheng Zhao ◽  
Qi You Liu ◽  
Yun Bo Zhang
Keyword(s):  
Phylogenetic Analysis ◽  
Species Identification ◽  
Contaminated Soil ◽  
Bacterial Strain ◽  
Degradation Efficiency ◽  
Genetic Identification ◽  
Microbial Consortia ◽  
Bacterial Strains ◽  
Bacterial Consortia ◽  
Degrading Bacteria

The biodegradation abilities of 10 dibenzothiophene degrading microbial consortia isolated from contaminated soil were investigated. 5 highly efficient dibenzothiophene degrading bacterial strains were obtained from the consortium LKY10 by screening on LB-agar plates.The bacterial strain LKY10-5 reduced more than 90% of dibenzothiophene with 40 mg•L-1concentration, and had higher degradation efficiency than enriched bacterial consortia in 7 days of cultivation. According to species identification and phylogenetic analysis, strain LKY10-1 and LKY10-3 belonged to Actinobacteria and could be included in Rhodococcus and Cellulosimicrobium genus, LKY10-5 and LKY10-6 belonged to Proteobacteria and could be included in Pseudomonas and Devosia genus, and LKY10-13 could be included in Lysinibacillus genus and belonged to Firmicutes.

scholarly journals Novel carbonatogenic bacterial strain isolated from limestone quarry in East Java, Indonesia to improve concrete performance

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
Enny Zulaika ◽  
Muhammad Andry Prio Utomo ◽  
Ajeng Selvyana Pangestu ◽  
Nur Hidayatul Alami ◽  
Maya Shovitri ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Concrete Strength ◽  
Concrete Structures ◽  
Maximum Load ◽  
Rrna Gene ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
Limestone Quarry ◽  
Crystal Properties

Abstract. Zulaika E, Utomo MAP, Pangestu AS, Alami NH, Shovitri M, Prasetyo EN, Setiawan E, Luqman A, Kuswytasari ND, Irawan C. 2021. Novel carbonatogenic bacterial strain isolated from limestone quarry in East Java, Indonesia to improve concrete performance. Biodiversitas 22: 3890-3898. Carbonatogenic bacteria can precipitate CaCO3 in the form of calcite, aragonite, or vaterite. Calcite has the potential to be applied for strengthening concrete structures. This research aims to explore several new bacterial strains that can precipitate calcium carbonate leading to produce calcite and could be useful for strengthening concrete structures. Soil and stalactite samples were taken from a well-known limestone quarry in East Java, Indonesia. The isolated bacteria species were identified using 16S rRNA gene sequences. CaCO3 crystal properties were characterized using X-Ray Diffraction and Scanning Electron Microscopy. Six novels isolated CaCO3 precipitating bacterial strains; Bacillus huizhouensis JA1; B. galactosidilyticus JB3; B. niacini AK4, B. lentus SU1, Lysinibacillus macroides JB2, and Sporosarcina soli JA4 were successfully isolated and have the potential to enhance concrete strength. All isolates were able to produce CaCO3 in calcite form except B. galactosidilyticus JB3. The experimental concrete with the addition of bacterial cells showed higher compressive strength and maximum load compared to control concrete and met the requirements for building construction so that it could be applied for building structure materials.

scholarly journals Isolation and characterisation of endocrine disruptor nonylphenol-using bacteria from South Africa

2017 ◽  
Vol 113 (5/6) ◽  
Author(s):  
Lehlohonolo B. Qhanya ◽  
Ntsane T. Mthakathi ◽  
Charlotte E. Boucher ◽  
Samson S. Mashele ◽  
Chrispian W. Theron ◽  
...  
Keyword(s):  
South Africa ◽  
Bacterial Species ◽  
Endocrine Disrupting Chemicals ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Mpumalanga Province ◽  
Synthetic Chemicals ◽  
Phylogenetic Identification ◽  
Rrna Gene Sequencing

Endocrine disrupting chemicals (EDCs) are synthetic chemicals that alter the function of endocrine systems in animals including humans. EDCs are considered priority pollutants and worldwide research is ongoing to develop bioremediation strategies to remove EDCs from the environment. An understanding of indigenous microorganisms is important to design efficient bioremediation strategies. However, much of the information available on EDCs has been generated from developed regions. Recent studies have revealed the presence of different EDCs in South African natural resources, but, to date, studies analysing the capabilities of microorganisms to utilise/degrade EDCs have not been reported from South Africa. Here, we report for the first time on the isolation and enrichment of six bacterial strains from six different soil samples collected from the Mpumalanga Province, which are capable of utilising EDC nonylphenol as a carbon source. Furthermore, we performed a preliminary characterisation of isolates concerning their phylogenetic identification and capabilities to degrade nonylphenol. Phylogenetic analysis using 16S rRNA gene sequencing revealed that four isolates belonged to Pseudomonas and the remaining two belonged to Enterobacteria and Stenotrophomonas. All six bacterial species showed degradation of nonylphenol in broth cultures, as HPLC analysis revealed 41–46% degradation of nonylphenol 12 h after addition. The results of this study represent the beginning of identification of microorganisms capable of degrading nonylphenol, and pave the way for further exploration of EDC-degrading microorganisms from South Africa.

scholarly journals Thalassolituus oleivorans gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons

2004 ◽  
Vol 54 (1) ◽  
pp. 141-148 ◽  
Author(s):  
Michail M. Yakimov ◽  
Laura Giuliano ◽  
Renata Denaro ◽  
Ermanno Crisafi ◽  
Tatiana N. Chernikova ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Gene Sequence ◽  
Sea Water ◽  
Enrichment Culture ◽  
Sequence Similarity ◽  
Bacterial Species ◽  
Rrna Gene ◽  
Pseudomonas Oleovorans ◽  
Rrna Gene Sequence ◽  
Gene Sequence Analysis

An aerobic, heterotrophic, Gram-negative, curved bacterial strain, designated MIL-1T, was isolated by extinction dilution from an n-tetradecane enrichment culture that was established from sea water/sediment samples collected in the harbour of Milazzo, Italy. In the primary enrichment, the isolate formed creamy-white, medium-sized colonies on the surface of the agar. The isolate did not grow in the absence of NaCl; growth was optimal at 2·7 % NaCl. Only a narrow spectrum of organic compounds, including aliphatic hydrocarbons (C7–C20), their oxidized derivatives and acetate, were used as growth substrates. The isolate was not able to grow under denitrifying conditions. The DNA G+C content and genome size of strain MIL-1T were estimated to be 53·2 mol% and 2·2 Mbp, respectively. The major cellular and phospholipid fatty acids were palmitoleic, palmitic and oleic acids (33·5, 29·5 and 11·0 % and 18, 32 and 31 %, respectively). 3-Hydroxy lauric acid was the only hydroxy fatty acid detected. Thirteen different compounds that belonged to two types of phospholipid (phosphatidylethylamine and phosphatidylglycerol) were identified. 16S rRNA gene sequence analysis revealed that this isolate represents a distinct phyletic lineage within the γ-Proteobacteria and has about 94·4 % sequence similarity to Oceanobacter kriegii (the closest bacterial species with a validly published name). The deduced protein sequence of the putative alkane hydrolase, AlkB, of strain MIL-1T is related to the corresponding enzymes of Alcanivorax borkumensis and Pseudomonas oleovorans (81 and 80 % similarity, respectively). On the basis of the analyses performed, Thalassolituus oleivorans gen. nov., sp. nov. is described. Strain MIL-1T (=DSM 14913T=LMG 21420T) is the type and only strain of T. oleivorans.

scholarly journals Characterization of Phascolarctobacterium succinatutens sp. nov., an Asaccharolytic, Succinate-Utilizing Bacterium Isolated from Human Feces

2011 ◽  
Vol 78 (2) ◽  
pp. 511-518 ◽  
Author(s):  
Yohei Watanabe ◽  
Fumiko Nagai ◽  
Masami Morotomi
Keyword(s):  
Bacterial Species ◽  
Short Chain Fatty Acids ◽  
Pcr Analysis ◽  
Rrna Gene ◽  
Gi Tract ◽  
Bacterial Strains ◽  
High Sequence Identity ◽  
Healthy Human ◽  
Content Type

ABSTRACTIsolation, cultivation, and characterization of the intestinal microorganisms are important for understanding the comprehensive physiology of the human gastrointestinal (GI) tract microbiota. Here, we isolated two novel bacterial strains, YIT 12067Tand YIT 12068, from the feces of healthy human adults. Phylogenetic analysis indicated that they belonged to the same species and were most closely related toPhascolarctobacterium faeciumACM 3679T, with 91.4% to 91.5% 16S rRNA gene sequence similarities, respectively. Substrate availability tests revealed that the isolates used only succinate; they did not ferment any other short-chain fatty acids or carbohydrates tested. When these strains were cocultured with the xylan-utilizing and succinate-producing bacteriumParaprevotella xylaniphilaYIT 11841T, in medium supplemented with xylan but not succinate, their cell numbers became 2 to 3 orders of magnitude higher than those of the monoculture; succinate became undetectable, and propionate was formed. Database analysis revealed that over 200 uncultured bacterial clones from the feces of humans and other mammals showed high sequence identity (>98.7%) to YIT 12067T. Real-time PCR analysis also revealed that YIT 12067T-like bacteria were present in 21% of human fecal samples, at an average level of 3.34 × 108cells/g feces. These results indicate that YIT 12067T-like bacteria are distributed broadly in the GI tract as subdominant members that may adapt to the intestinal environment by specializing to utilize the succinate generated by other bacterial species. The phylogenetic and physiological properties of YIT 12067Tand YIT 12068 suggest that these strains represent a novel species, which we have designatedPhascolarctobacterium succinatutenssp. nov.

scholarly journals Biodiversity of Actinomycetes from Heavy Metal Contaminated Technosols

2021 ◽  
Vol 9 (8) ◽  
pp. 1635
Author(s):  
Michaela Cimermanova ◽  
Peter Pristas ◽  
Maria Piknova
Keyword(s):  
Heavy Metal ◽  
Bacterial Colonization ◽  
Bacterial Species ◽  
Chemical Properties ◽  
Metal Resistance ◽  
Rrna Gene ◽  
Toxic Substances ◽  
Bacterial Strains ◽  
16S Rrna Gene Analysis ◽  
Genus Streptomyces

Technosols are artificial soils generated by diverse human activities and frequently contain toxic substances resulting from industrial processes. Due to lack of nutrients and extreme physico-chemical properties, they represent environments with limited bacterial colonization. Bacterial populations of technosols are dominated usually by Actinobacteria, including streptomycetes, known as a tremendous source of biotechnologically important molecules. In this study, the biodiversity of streptomycete-like isolates from several technosols, mainly mine soils and wastes (landfills and sludge) in Slovakia, was investigated. The combination of basic morphological and biochemical characterisations, including heavy metal resistance determination, and molecular approaches based on 16S rRNA gene analysis were used for the identification of the bacterial strains. From nine isolates of Actinobacteria collected from different habitats, one was found to represent a new species within the Crossiella genus. Eight other isolates were assigned to the genus Streptomyces, of which at least one could represent a new bacterial species. Some isolates showed high resistance to Pb, Zn, Cu or Ni. The most tolerated metal was Pb. The results obtained in this study indicate that technosols are a prospective source of new actinomycete species resistant to heavy metals what underlines their bioremediation potential.

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