Ocurrence and risk factors associated with Mycoplasma agalactiae infection in dairy goat herds of Paraíba State, Brazil

ABSTRACT: Mycoplasmosis is a disease that may cause severe economical losses in goat and sheep herds, and it is associated with mastitis, polyarthritis, agalactia, conjunctivitis, pneumonia and reproductive failure. The objective of this study was to determine the occurrence of Mycoplasma agalactiae in milk samples and investigate the main risk factors associated with infection in goats from farms of the state of Paraíba, Brazil. For Mycoplasma agalactiae diagnosis, 251 milk samples were submitted to DNA extraction using a commercially available kit, following the manufacturer’s instructions and Polymerase Chain Reaction (PCR) was performed. In addition, questionnaires were applied to identify the main risk factors associated with contagious agalactia. Out of the two hundred fifty-one samples analyzed, 50 (19.9%, I.C. 15.1-25.4%) were PCR positive for M. agalactiae. In the risk factors analysis, some associations were observed for the following variables: size of the herd (P<0.001, OR=7.1, I.C. 2.4-20.6), replacement of farm animals (P<0.001, OR=4.7, I.C. 1.8-12.2) and participation of animals in fairs and exhibitions (P=0.029, OR=2.0, I.C.1.0-3.9). The results allowed confirming the occurrence of Mycoplasma agalactiae in milk samples of goats from Paraíba. Therefore, it is strictly necessary to monitor dairy goat flocks and to raise the awareness of farmers about the economic importance of the disease, since it causes severe economic losses for producers of the state. Identification of risk factors is essential for adoption of control measures and for the correction of the management factors in farms where there are animals with positive diagnosis, avoiding, so, pathogen dissemination.

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Mycoplasma agalactiae in Dairy Goat Flocks Bred in State of Ceará in Association with Caprine Arthritis Encephalitis Virus

Acta Scientiae Veterinariae ◽

10.22456/1679-9216.86091 ◽

2018 ◽

Vol 46 (1) ◽

pp. 7

Author(s):

Renato Mesquita Peixoto ◽

Alice Andrioli ◽

Raymundo Rizaldo Pinheiro ◽

Francisco Selmo Fernandes Alves ◽

Vanderlan Warlington Souza Dos Santos ◽

...

Keyword(s):

Milk Production ◽

Clinical Signs ◽

Small Ruminants ◽

The State ◽

Encephalitis Virus ◽

Control Measures ◽

Dairy Goat ◽

Economic Losses ◽

Caprine Arthritis Encephalitis Virus ◽

Mycoplasma Agalactiae

Background: Contagious agalactia is an infectious disease caused by Mycoplasma agalactiae (M. agalactiae) that occurs in small ruminants leading to productive and economic losses. Due to the similarity of clinical signs presented in Caprine Arthritis Encephalitis (CAE), which is a viral disease, a differential diagnosis is important. Therefore, this study aimed to investigate the presence of anti-Mycoplasma agalactiae antibodies in dairy goat flocks in Ceará State and possible correlation with CAE.Materials, Methods & Results: The research was performed in four mesoregions in Ceará State (Metropolitan Region of Fortaleza-MRF; Northeast Ceará - NeC; North Ceará - NC; Sertões in Ceará - SC), from which 16 productions located in 10 cities with the highest representativeness for goat milk production within the State or mesoregion were sampled. A total of 417 females and 69 males (486 animals) of breeds with dairy production aptitude, pure or crossbreed, maintained in semi-intensive or intensive systems, were tested. Blood serum was obtained by venipuncture of the jugular vein with vacuum pressure syringe followed by centrifugation at 1,500 g for 10min. Antibodies against the caprine arthritis encephalitis virus (CAEV) were detected with micro technique of agarose gel immunodiffusion (AGID) and Western Blot (WB). The anti-Mycoplasma agalactiae antibodies were detected with commercial kit of enzymatic immunoassay (IDEXX Laboratories™). Seroprevalence of M. agalactiae in dairy goat flocks in Ceará State was 0.62% (3/486). From the total of 16 visited productions, 18.75% (3/16) had seropositive animals for M. agalactiae located in MRF, NC and SC mesoregions. CAE was diagnosed in 56.25% (9/16) of productions with AGID and in 81.25% (13/16) with WB. In addition, 5.2% (25/486) of animals were seropositive for CAE with AGID and 16.6% (80/486) with WB. Animals that reacted positive for M. agalactiae were all females of pure breed with milk production aptitude in distinct mesoregions submitted to intensive rearing system. None of these animals was positive in neither test (AGID or WB) for CAE. Therefore, no correlation of results obtained in diagnosis of M. agalactiae by ELISA and CAEV by AGID or WB (P < 0.05) was identified. However, two out of three productions that were positive for M. agalactiae presented positive results for CAEV with frequencies of 10% and 20%.Discussion: Seroprevalence of M. agalactiae in Ceará State was low in comparison with other Brazilian states and even other countries. However, the presence of the pathogen in more than one mesoregion indicates that the disease occurs in different locations within the State. Therefore, flocks in Ceará are susceptible to the infection, which may be favored by uncontrolled commerce that occurs with deficient surveillance, associated with the importation of animals to improve flock genetic quality. The presence of the pathogen in dairy goats may contribute to significant losses in the local production. On the other hand, CAE was diagnosedin nearly all productions proving the dissemination of this lentivirus infection among dairy goat flocks in Ceará State. Although an association between these diseases was not identified, the presence of a retrovirus in the organism may favor co-infection with another micro-organism, promoting the deficiency in the immune system of the host. In conclusion, M. agalactiae is present in different mesoregions of the Ceará State and control measures should be adopted in short term to prevent pathogen dissemination and, consequently reduce economic and productive losses in the local dairy goat production. No correlation was identified between the prevalence of infection by CAEV and M. agalactiae in this study.Keywords: correlation, diagnosis, caprine lentivirus, mycoplasmosis.

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Mycoplasma agalactiae in Dairy Goat Flocks Bred in State of Ceará in Association with Caprine Arthritis Encephalitis Virus

Acta Scientiae Veterinariae ◽

10.22456/1679-9216.81885 ◽

2018 ◽

Vol 46 (1) ◽

Cited By ~ 1

Author(s):

Renato Mesquita Peixoto ◽

Alice Andrioli ◽

Raymundo Rizaldo Pinheiro ◽

Francisco Selmo Fernandes Alves ◽

Vanderlan Warlington Souza Dos Santos ◽

...

Keyword(s):

Milk Production ◽

Clinical Signs ◽

Small Ruminants ◽

The State ◽

Encephalitis Virus ◽

Control Measures ◽

Dairy Goat ◽

Economic Losses ◽

Caprine Arthritis Encephalitis Virus ◽

Mycoplasma Agalactiae

Background: Contagious agalactia is an infectious disease caused by Mycoplasma agalactiae (M. agalactiae) that occurs in small ruminants leading to productive and economic losses. Due to the similarity of clinical signs presented in Caprine Arthritis Encephalitis (CAE), which is a viral disease, a differential diagnosis is important. Therefore, this study aimed to investigate the presence of anti-Mycoplasma agalactiae antibodies in dairy goat flocks in Ceará State and possible correlation with CAE.Materials, Methods & Results: The research was performed in four mesoregions in Ceará State (Metropolitan Region of Fortaleza- MRF; Northeast Ceará - NeC; North Ceará - NC; Sertões in Ceará - SC), from which 16 productions located in 10 cities with the highest representativeness for goat milk production within the State or mesoregion were sampled. A total of 417 females and 69 males (486 animals) of breeds with dairy production aptitude, pure or crossbreed, maintained in semi-intensive or intensive systems, were tested. Blood serum was obtained by venipuncture of the jugular vein with vacuum pressure syringe followed by centrifugation at 1,500 g for 10min. Antibodies against the caprine arthritis encephalitis virus (CAEV) were detected with micro technique of agarose gel immunodiffusion (AGID) and Western Blot (WB). The anti-Mycoplasma agalactiae antibodies were detected with commercial kit of enzymatic immunoassay (IDEXX Laboratories™). Seroprevalence of M. agalactiae in dairy goat flocks in Ceará State was 0.62% (3/486). From the total of 16 visited productions, 18.75% (3/16) had seropositive animals for M. agalactiae located in MRF, NC and SC mesoregions. CAE was diagnosed in 56.25% (9/16) of productions with AGID and in 81.25% (13/16) with WB. In addition, 5.2% (25/486) of animals were seropositive for CAE with AGID and 16.6% (80/486) with WB. Animals that reacted positive for M. agalactiae were all females of pure breed with milk production aptitude in distinct mesoregions submitted to intensive rearing system. None of these animals was positive in neither test (AGID or WB) for CAE. Therefore, no correlation of results obtained in diagnosis of M. agalactiae by ELISA and CAEV by AGID or WB (P < 0.05) was identified. However, two out of three productions that were positive for M. agalactiae presented positive results for CAEV with frequencies of 10% and 20%.Discussion: Seroprevalence of M. agalactiae in Ceará State was low in comparison with other Brazilian states and even other countries. However, the presence of the pathogen in more than one mesoregion indicates that the disease occurs in different locations within the State. Therefore, flocks in Ceará are susceptible to the infection, which may be favored by uncontrolled commerce that occurs with deficient surveillance, associated with the importation of animals to improve flock genetic quality. The presence of the pathogen in dairy goats may contribute to significant losses in the local production. On the other hand, CAE was diagnosed in nearly all productions proving the dissemination of this lentivirus infection among dairy goat flocks in Ceará State. Although an association between these diseases was not identified, the presence of a retrovirus in the organism may favor co-infection with another micro-organism, promoting the deficiency in the immune system of the host. In conclusion, M. agalactiae is present in different mesoregions of the Ceará State and control measures should be adopted in short term to prevent pathogen dissemination and, consequently reduce economic and productive losses in the local dairy goat production. No correlation was identified between the prevalence of infection by CAEV and M. agalactiae in this study.Keywords: correlation, diagnosis, caprine lentivirus, mycoplasmosis.

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Rabies Ecology in Bauchi State, Nigeria

Journal of Veterinary and Biomedical Sciences ◽

10.36108/jvbs/9102.20.0120 ◽

2019 ◽

Vol 2 (1) ◽

pp. 5-12

Author(s):

Bolajoko Muhammad-Bashir ◽

Yakubu Joel Atuman

Keyword(s):

Risk Factors ◽

Cost Effective ◽

The State ◽

Control Measures ◽

P Value ◽

Public Health Implication ◽

Factors Associated ◽

Canine Rabies ◽

Dog Ownership ◽

Dog Owners

Despite availability of safer and cost-effective treatments and vaccines, rabies is still considered among the most fatal viral zoonotic disease worldwide. The epidemiology of rabies is directly associated with the ecology of the reservoirs which needs better understanding to underpin appropriate control measures. There are only few reported attempts made towards understanding the ecology of dog and rabies cases in Nigeria. Although dogs are tolerated and kept in Bauchi State, the risk factors associated with dog ownership in the state, remain to be studied. This study is aimed at determining the risk factors associated with dog ownership in Bauchi state and to evaluate the degree with which the presence or absence of the identified factors can increase or decrease risk of rabies cases in the state. A questionnaire-based survey was conducted amongst dog owners across the state. Basic descriptive analyses were carried out in Microsoft Excel 2016 and IBM SPSS 21 was used to determine the relative risk (RR) of rabies occurrence in households across the state when exposed to each of the identified risk factors where P-value was set at 0.05. A total of 80 questionnaires were completed and received from the respondents. The calculated RR, revealed that the practice of extensive system of management increases the risk of canine rabies amongst dogs of the state by 80% and knowledge of dog owners about canine rabies reduces the risk of canine rabies by 27%. This study confirmed that dogs are owned and tolerated but poorly managed under extensive management system in Bauchi state with increasing risks of rabies spread due to low vaccination coverage and lack of practical application of knowledge on the dangers of rabies amongst the populace. The public health implication of this situation and the need for concerted efforts for sustainable control of rabies in the state is discussed

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Epidemiological characterization and risk factors associated with Leptospira infection in dogs from rural settlements in the semi-arid region of Northeast Brazil

Semina Ciências Agrárias ◽

10.5433/1679-0359.2017v38n4supl1p2531 ◽

2017 ◽

Vol 38 (4Supl1) ◽

pp. 2531

Author(s):

José Dêvede Da Silva ◽

Demmya Haryssam Menezes Melo ◽

Érico Luiz de Barros Correia ◽

José Romero Alexandre Alves ◽

Diego Figueiredo Costa ◽

...

Keyword(s):

Risk Factors ◽

The State ◽

Control Measures ◽

Northeast Brazil ◽

Rural Settlements ◽

Factors Associated ◽

Sources Of Infection ◽

Semi Arid Region ◽

Sanitary Conditions ◽

Epidemiological Characterization

Rural settlements are defined as family-run agrarian spaces. Their purpose, based on public policies, is to strengthen family agriculture. They are peculiar environments that can predispose to the risk of diseases, especially zoonotic diseases, due to close natural contact with animals that can be sources of infection. The aim of this study was to determine the frequency of anti-Leptospira antibodies in dogs from rural settlements in the state of Paraíba, Northeast Brazil, and to identify the risk factors associated with infection. A total of 306 dogs were sampled and Leptospira screening was performed using the microscopic agglutination test. In all, 27 of 306 (8.82%; 95% CI = 6.13-12.5%) dogs were seropositive for Leptospira spp. The most frequent serotype was Pomona. Street access (odds ratio = 3.53, p = 0.012) was indicated as a risk factor for infection. The results indicate that leptospirosis is present in dogs from rural settlements in the state of Paraíba, Northeast Brazil. This reinforces the need to limit contact between dogs and wild reservoirs and perform serological monitoring of swine. It also reinforces the need for implementation of control measures such as improvements in hygienic-sanitary conditions in the settlements, including the construction of pigsties away from homes, and the introduction of vaccination to reduce the occurrence of this disease, which will prevent transmission to humans and animals.

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Prevalence and risk factors associated to Eimeria spp. infection in unweaned alpacas (Vicugna pacos) from Southern Peru

Acta Parasitologica ◽

10.1515/ap-2016-0008 ◽

2016 ◽

Vol 61 (1) ◽

Cited By ~ 2

Author(s):

Pablo Díaz ◽

Rosario Panadero ◽

Rosalía López ◽

Aida Cordero ◽

Ana Pérez-Creo ◽

...

Keyword(s):

Risk Factors ◽

Geographical Area ◽

Eimeria Species ◽

Control Measures ◽

Economic Losses ◽

Herd Prevalence ◽

Factors Associated ◽

Southern Peru ◽

Eimeria Spp ◽

Eimeria Infection

AbstractA total of 350 faecal samples from unweaned alpacas over 3 months of age were collected from 23 herds in order to determine the prevalence of Eimeria spp. in Southern Peru and to identify the risk factors associated to Eimeria infection in young alpacas. Samples were examined by a flotation technique and the identification of risk factors was assessed by a logistic regression analysis. Sixty four percent of the examined animals shed Eimeria oocysts; herd prevalence was 96%, with an intra-herd prevalence of 60% (range 5.9-100%). Five different Eimeria species were identified, being E. lamae (91%), E. alpacae (87%) and E. punoensis (78%) the most prevalent; E. macusaniensis (35%) and E. ivitaensis (13%) were less common. Mixed-species infections were more frequent (78%) than single infections (22%). E. lamae was the most common monospecific infection and E. lamae/E. alpacae the most frequent association. The geographical area has a significant effect on Eimeria infection rates (74.9% wet Puna vs 37.4% dry Puna) as well as the breeding system (65.1% traditional vs 63.8% modern). In contrast, the sex of the animals (64.6% males vs 64.0% females) showed no influence on the prevalence of infection by Eimeria. The high prevalence found at both individual and herd level and the common presence of highly pathogenic Eimeria species may lead to important economic losses for alpaca breeders and could require the implementation of suitable control measures.

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Risk factors associated with BoHV-1 and BVDV seropositivity in buffaloes (Bubalus bubalis) from the State of Paraiba, Northeastern Brazil

Semina Ciências Agrárias ◽

10.5433/1679-0359.2016v37n4p1927 ◽

2016 ◽

Vol 37 (4) ◽

pp. 1929 ◽

Cited By ~ 1

Author(s):

Leise Gomes Fernandes ◽

Carla Lauise Rodrigues Menezes Pimenta ◽

Edviges Maristela Pituco ◽

Arthur Willian de Lima Brasil ◽

Sérgio Santos de Azevedo

Keyword(s):

Risk Factors ◽

Risk Factor ◽

Odds Ratio ◽

The State ◽

Northeastern Brazil ◽

Economic Losses ◽

Prevention Measures ◽

Herpesvirus Type ◽

Diarrhea Virus ◽

Factors Associated

This study aimed to identify the risk factors associated with Bovine Herpesvirus Type 1 (BoHV-1) and Bovine Viral Diarrhea Virus (BVDV) infections in buffaloes in the State of Paraíba, Northeastern Brazil, using 136 female buffaloes ? 24 months old from 14 herds. For the serological diagnosis of the BoHV-1 and BVDV infections, the virus-neutralization test (VN) was used and in each herd, an epidemiological questionnaire was applied in order to obtain data to be used in the risk factor analysis. Of the 136 animals 86 (63.2%) were positive for BoHV-1 and 12 (8.8%) for BVDV. The presence of watering points (odds ratio = 17.68; 95% CI = 3.74 – 83.04) was identified as risk factor for BoHv-1, and animal purchasing was a risk factor for both BoHV-1 (odds ratio = 68.64; 95% CI = 12.28 – 383.73) and BVDV (odds ratio = 3.64; 95% CI = 1.04 – 12.76). The results from the present study showed an evidence of the presence of BoHV-1 and BVDV infections in buffaloes from the State of Paraíba, Northeastern Brazil. It is suggested that control and prevention measures should be adopted, such as the use of diagnostic tests prior to animal purchasing and the use of vaccines to avoid the introduction of infected animals into the herds and the consequent dissemination of the infections, minimizing economic losses.

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Neospora caninum infection in cattle in the state of Amazonas, Brazil: seroprevalence, spatial distribution and risk factors

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-296120201083 ◽

2021 ◽

Vol 30 (1) ◽

Author(s):

Paulo Cesar Gonçalves de Azevedo Filho ◽

Müller Ribeiro-Andrade ◽

Jomel Francisco dos Santos ◽

Arthêmio Coelho dos Reis ◽

José Wilton Pinheiro Júnior ◽

...

Keyword(s):

Risk Factors ◽

Spatial Distribution ◽

Production Systems ◽

Neospora Caninum ◽

The State ◽

Control Measures ◽

Risk Indicators ◽

Caninum Infection ◽

Factors Associated ◽

Madeira River

Abstract Livestock in the Amazon has grown significantly and, although neosporosis in cattle has been reported worldwide, there is no information about N. caninum in production systems in the state of Amazonas. The objective of this study was to determine the prevalence of anti-Neospora caninum antibodies in cattle, their spatial distribution and the risk factors associated with N. caninum infection in the state of Amazonas. Questionnaires were applied to farmers to assess risk factors associated with N. caninum infection. Blood samples were collected from 1,073 animals on 47 farms in 33 municipalities in the four Amazonian subpopulations. IgG anti-N.caninum antibodies were detected by the indirect fluorescence test, with a general prevalence of 30.2%, being seropositive in 43 farms (91.5%), with prevalence ranging from 2.2% to 69.2%. The highest number of high density points was found in subpopulation 3 (municipality of Apuí and other municipalities on the Madeira River and affluent). It was concluded that N. caninum is present with high seroprevalence values, when compared to other cattle producing states in the Amazon region of Brazil. The identified factors can be used as risk indicators so that control measures can be implemented to prevent infection by N. caninum in these herds.

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Prevalência e fatores de risco da Leucose Enzoótica Bovina em rebanhos bovinos da microrregião de Teresina, estado do Piauí, Brasil

Revista Agraria Academica ◽

10.32406/v3n62020/6-15/agrariacad ◽

2020 ◽

Vol 3 (6) ◽

pp. 6-15

Author(s):

Pedro Henrique Viana Rebêlo ◽

◽

Flávio de Sousa Oliveira ◽

Rafael Gabino Cavalcante ◽

Francisco das Chagas Cardoso Junior ◽

...

Keyword(s):

Risk Factors ◽

Prevalence Rate ◽

Prevention And Control ◽

The State ◽

Control Measures ◽

Agar Gel ◽

Blood Samples ◽

Factors Associated ◽

Prevention And Control Measures ◽

And Control

The present work aims to estimate the seroprevalence of Bovine Enzootic Leukosis - LEB and to identify the risk factors associated with seroreactivity for infection by the LEB virus, in cattle from herds raised in the Teresina Microregion, in the State of Piaui, Brazil. 420 blood samples were examined in 14 municipalities belonging to the microregion, the technique used for the research was Immunodiffusion in Agar Gel (IDGA). There was a prevalence rate of 19.52% (82/420) of seroreagent animals, with the occurrence of at least one animal positive for LEB in each herd evaluated. The results show that prevention and control measures are necessary for better surveillance of herds.

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Epidemiological characterization and risk factors associated with Leptospira infection in dogs from rural settlements in the semi-arid region of Northeast Brazil

Semina Ciências Agrárias ◽

10.5433/1679-0359.2017v38n4suplp2531 ◽

2017 ◽

Vol 38 (4Supl1) ◽

pp. 2531

Author(s):

José Dêvede Da Silva ◽

Demmya Haryssam Menezes Melo ◽

Érico Luiz de Barros Correia ◽

José Romero Alexandre Alves ◽

Diego Figueiredo Costa ◽

...

Keyword(s):

Risk Factors ◽

The State ◽

Control Measures ◽

Northeast Brazil ◽

Rural Settlements ◽

Factors Associated ◽

Sources Of Infection ◽

Semi Arid Region ◽

Sanitary Conditions ◽

Epidemiological Characterization

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Seroprevalence and risk factors associated with infection by Neospora caninum of dairy cattle in the state of Alagoas, Brazil

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2012001000011 ◽

2012 ◽

Vol 32 (10) ◽

pp. 1009-1013 ◽

Cited By ~ 9

Author(s):

Maria E. Sousa ◽

J.N. Porto Wagnner ◽

Pedro P.F. Albuquerque ◽

Orestes L. Souza Neto ◽

Eduardo B. Faria ◽

...

Keyword(s):

Risk Factors ◽

Dairy Cattle ◽

Indirect Immunofluorescence ◽

Neospora Caninum ◽

Infection Risk ◽

Control Measures ◽

Risk Indicators ◽

Blood Samples ◽

Factors Associated ◽

Serological Investigation

The objective of this study was to investigate the prevalence of anti-Neospora caninum antibodies in cattle from milk producing farms of the microregion of Batalha, state of Alagoas, Brazil, as well as to identify the risk factors associated with the infection. Blood samples were collected from 1,004 cattle of 17 farms for the serological investigation regarding the presence of anti-N. caninum antibodies by the Indirect Immunofluorescence Reaction Technique (IMRT). From the total amount of samples analyzed, 77/1,004 (7.67%) were positive and 927/1,004 (92.33%) were negative. The logistical regression identified that cattle from farms without consortium breeding have an infection risk 6.33 (p<0.001; C.I. 2.89-13.10) times higher than cattle from farms with that type of breeding. Cattle from farms where the aborted fetuses are not adequately buried have an infection risk 3.04 (p<0.001; C.I. 1.64-5.63) times higher than cattle from farms with adequate destination of these fetuses. Infection by N. caninum occurs in cattle of the investigated region. The factors identified in our study can be used as risk indicators, so that control measures could be implemented to avoid infection by N. caninum in the herds of this region.

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