Effect of peracetic acid used as single irrigant on the smear layer, adhesion, and penetrability of AH Plus

AIM: The aim of current research was to find out the sealing ability of two commonly used sealers in the presence and absence of smear layer at different time interval. MATERIAL AND METHODS: Total of 180 single rooted vital teeth were used. Transversal section was made with the help of digital slow speed cutting saw to divide the root and crown part. After removal of crown roots part was instrumented and prepared. The 180 teeth were randomly divided into two equal groups (n=90) 1 and 2. In group 1 the smear layer was kept intact but it was removed from group 2 with EDTA (17%). Group 1 was then divided into two sub-groups, A1, A2. Group 2 was again separated into two sub-groups, B1 and B2. Each sub-group contained of 45 samples. In sub-groups A1 and B1, AH Plus sealer, in sub-groups A2 and B2, Ketac-endo sealer and cold lateral condensation technique was used for obturation with gutta percha. All samples were kept in an incubator at 37°C for 24 hours, with help of nail polish/varnish all samples root surfaces was painted only excluding apical area after words each sub group were further divided in to three groups of 15 to represent immersion periods of 7,15 and 30 days. All samples were then kept in 5 % methylene blue dye solution at 37o c for their respective time periods. After specific time period, the roots of every group were cut longitudinally and evaluate under a stereomicroscopes to evaluate apical micro leakage in millimeter. Data was subjected to Repeated measure ANOVA with post-hoc analysis using Tukey and bonferroni tests. RESULTS: Overall analysis indicated significant reduction in sealing ability canals in with and without smear layer over different time periods (p <0.0001). CONCLUSION: Current study has concluded that AH plus sealer provides significantly better seal in the absence of smear layer than in the presence of smear layer in addition it gave better seal than Ketac-Endo sealer in the absence and presence of smear layer.

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An in vitro Evaluation of Microtensile Bond Strength of Resin-based Sealer with Dentin Treated with Diode and Nd:YAG Laser

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1297 ◽

2013 ◽

Vol 14 (2) ◽

pp. 183-187 ◽

Cited By ~ 4

Author(s):

G Anil Kumar ◽

Maneesha Das ◽

Sindhu Ramesh ◽

Surendranath Garapati

Keyword(s):

Bond Strength ◽

Nd:Yag Laser ◽

Root Canal ◽

Smear Layer ◽

Control Group ◽

Microtensile Bond Strength ◽

Group Iii ◽

Group I ◽

Ah Plus

ABSTRACT Background Smear layer is a negative factor which prevents adhesion of the filling material to the dentinal walls. Recent advances in dental research have incorporated lasers as a potential adjunct in root canal treatment by removing the smear layer before filling the root canal system, enhancing the adhesion of sealers to dentin and improving the sealing ability. Aim To evaluate the microtensile bond strength of AH-Plus resin-based sealer to dentin after treatment with 980 nm diode and 1,064 nm neodymium-doped:yttrium aluminum garnet (Nd:YAG) laser in vitro. Materials and methods Thirty specimens prepared for three groups namely group I (control), group II (980 nm diode–lased specimens) and group III (Nd:YAG–lased specimens). One tooth from each group was observed under scanning electron microscope for evaluation of intracanal root dentin morphology. Remaining specimens were used for making microsections by hard tissue microtome. Specimens for groups II and III were lased with 980 nm diode and 1,064 nm Nd:YAG laser. AH Plus sealer was applied onto specimens and mounted onto Instron universal testing machine for microtensile bond strength testing. Results were subjected to statistical analysis using one-way analysis of variance (ANOVA) and Tukey's test. Results Group III Nd:YAG had maximum mean microtensile bond strength values (11.558 ± 0.869), followed by group II diode (9.073 ± 0.468) and group I control (6.05 ± 0.036). Statistically significant differences were seen among all the groups. SEM analysis shows removal of smear layer in both groups II and III. Conclusion Both Nd:YAG and diode laser were more effective than control group in improving the microtensile bond strength of AH Plus sealer to dentin. Clinical significance Lasers have the potential to increase the adhesiveness of root canal sealer to dentin surface, thereby improving the quality of root canal obturation. How to cite this article Maneesha D, Anil KG, Sindhu R, Surendranath G, Deepak S. An in vitro Evaluation of Microtensile Bond Strength of Resin-based Sealer with Dentin Treated with Diode and Nd:YAG Laser. J Contemp Dent Pract 2013;14(2): 183-187.

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In vitro evaluation of coronal microleakage associated with two root obturation techniques

Stomatoloski glasnik Srbije ◽

10.2298/sgs0703184z ◽

2007 ◽

Vol 54 (3) ◽

pp. 184-190 ◽

Cited By ~ 1

Author(s):

Slavoljub Zivkovic ◽

Borislava David-Burlica ◽

Jelena Stanisic ◽

Sonja Stojcic ◽

Milica Medojevic-Jovanovic ◽

...

Keyword(s):

Nitrate Solution ◽

Silver Nitrate Solution ◽

Smear Layer ◽

Human Teeth ◽

Root Canals ◽

Gutta Percha ◽

Ah Plus ◽

The Difference ◽

Micrometer Scale

Aim: The purpose of this study was to determine the influence of the smear layer on the coronal microleakage in root canals obturated with laterally condensed cold gutta-percha or Thermafil and two root canal sealers. Materials and methods: Sixty extracted single-rooted human teeth with straight root canals were allocated to two experimental groups. The root canals were instrumented using the STEP-BACK technique, K reamers and K files and irrigated with 2.5 % NaOCl. The teeth in the first group were treated with 17% EDTA and 2.5 % NaOCl to remove the smear layer. The teeth in both groups were obturated either with laterally condensed cold guttapercha (28 teeth) or Thermafil gutta-percha with plastic cores (28 teeth) and RSA or AH-PLUS sealers. The specimens were evaluated for coronal microleakage using silver nitrate solution. The linear penetration of the dye was assessed using a binocular magnifying glass (x25), with an inserted micrometer scale. Results: The results showed low coronal microleakage in the group where the smear layer was removed in both the lateral compaction technique (RSA - 1.71mm and AHPLUS - 1.61mm) and the Thermafil groups - RSA (1.65mm) and AH-PLUS (1.66mm). In the group with the intact smear layer, greater microleakage was noticed in both groups, lateral compaction technique -RSA (3.06 mm) and AH-PLUS (2.83mm) and Thermafil technique -RSA (2.99mm) and AH-PLUS ( 2.83mm). The difference was statistically significant between the groups with and without the smear layer for both RSA (p< 0.001) and AH-PLUS sealers ( p<0.001). Conclusion: The present results suggest that the removal of the smear layer significantly improves the tightness of the coronal seal and reduces the coronal microleakage.

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Molhabilidade de um cimento endodôntico à base de resina epóxi na dentina tratada com diferentes protocolos de quelação

Dental Press Endodontics ◽

10.14436/2358-2545.11.3.024-030.oar ◽

2021 ◽

Vol 11 (3) ◽

pp. 24-30

Author(s):

Marcela Baraúna Magno ◽

Maíra do Prado ◽

Renata A. Simão ◽

Kawanne Ferreira Moraes Medeiros ◽

Patricia de Almeida Rodrigues ◽

...

Keyword(s):

Smear Layer ◽

Ah Plus

Objetivo: Avaliar a molhabilidade do cimento AH Plus em contato com a dentina radicular após diferentes protocolos quelantes envolvendo ácido etidrônico (HEBP) e o EDTA. Métodos: Cinquenta e seis fatias de dentina radicular foram utilizadas e irrigadas com 5.25% de hipoclorito de sódio (NaOCl) ou com uma mistura de 5.25%NaOCl/18%HEBP para simular a irrigação durante o preparo químico-mecânico. As amostras irrigadas com NaOCl foram divididas em 5 grupos, de acordo com o agente quelante: G1- água destilada (AD); G2-17%EDTA; G3-17%EDTA+2.5%NaOCl; G4-18%HEBP; e G5-18%HEBP+2.5%NaOCl. As amostras irrigadas com a mistura de NaOCl/HEBP foram dividias em 2 grupos: G6-AD; G7-NaOCl/HEBP+2.5%- NaOCl. Todos os protocolos receberam irrigação de AD entre as substâncias irrigadoras e como lavagem final. O goniômetro Rame-Hart foi utilizado para mesurar o ângulo de contato entre a superfície da dentina radicular e o cimento. O teste estatístico Kruskal-Wallis e Dunn foram aplicados (p<0.05). Resultados: Os grupos onde a smear layer foi removida apresentaram menor ângulo de contato (p<0.05), com exceção do G7. O G6 apresentou o menor ângulo de contato do AH Plus, entretanto, a irrigação final com NaOCl (G7) aumentou significativamente o ângulo de contato. G2 e G4 apresentaram comportamento similar e a irrigação final com NaOCL (G3 e G4) não modificou a molhabilidade da dentina. Conclusão: O tratamento da dentina radicular com a mistura NaOCl/HEBP, quando usada como irrigante, conferiu boa molhabilidade desta superfície ao cimento AH Plus.

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COMPARATIVE EVALUATION OF THE EFFECT OF SMEAR LAYER REMOVAL WITH EDTA AND ND: YAG LASER ON THE APICAL MICRO LEAKAGE OF TWO EPOXY RESIN BASED ROOT CANAL SEALERS - AN INVITRO STUDY.

Journal of Biomedical and Pharmaceutical Research ◽

10.32553/jbpr.v8i3.626 ◽

2019 ◽

Vol 8 (3) ◽

Author(s):

Dhanalakshmi Subramanian ◽

Mahendran Kavitha ◽

Anuraag Gurtu ◽

Bakthavatchalam Balakrishnan ◽

Mahalakshmi Jayaraman

Keyword(s):

Nd:Yag Laser ◽

Smear Layer ◽

Maxillary Central Incisor ◽

Layer Removal ◽

Group 4 ◽

Significant Difference ◽

Ah Plus ◽

Group 3 ◽

Group 5 ◽

Group 2

Aim: To compare the effect of smear layer removal with 17% EDTA and Nd:YAG laser on the apical microleakage of two resin based sealers. Materials and Methods: Sixty freshly extracted maxillary central incisor teeth with patent canals were selected. The teeth were debrided and stored in saline for 24 hours before use. The teeth were sectioned at CEJ & cleaning and shaping were done upto 50 size by step back technique. The following groups were analyzed. GROUP 1: Gutta percha (GP) + AH plus sealer without smear layer removal. GROUP 2: GP + RC seal sealer without smear layer removal. GROUP 3: GP + AH plus treated with 17% EDTA. GROUP 4: GP + RC seal treated with 17% EDTA. GROUP 5: GP + AH plus treated with Nd:YAG laser. GROUP 6: GP + RC seal treated with Nd:YAG laser. 8 samples were subjected to dye penetration study for apical microleakage & 2 samples were subjected to SEM in each group to show the effect of smear layer removal by EDTA and laser. The data were analyzed by using ANOVA and TUKEY-HSD test. Results: Groups 1 and 2 showed maximum microleakage , other groups showed lesser microleakage but there was no statistically significant difference between laser and EDTA treated Groups. Conclusion: EDTA and Nd:YAG laser can be effectively used to remove smear layer and showed less apical microleakage compared to non-treated groups. Keywords: 17% EDTA, Nd:YAG LASER , AH plus , RC sealer , Smear layer.

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Effects of ethylenediaminetetraacetic, etidronic and peracetic acid irrigation on human root dentine and the smear layer

International Endodontic Journal ◽

10.1111/j.1365-2591.2008.01514.x ◽

2009 ◽

Vol 42 (4) ◽

pp. 335-343 ◽

Cited By ~ 121

Author(s):

S. Lottanti ◽

H. Gautschi ◽

B. Sener ◽

M. Zehnder

Keyword(s):

Peracetic Acid ◽

Smear Layer ◽

Acid Irrigation

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Influence of Er: YAG laser irradiation on apical sealing of four different sealers

Brazilian Dental Journal ◽

10.1590/s0103-64402004000300005 ◽

2004 ◽

Vol 15 (3) ◽

pp. 190-193 ◽

Cited By ~ 8

Author(s):

Isabel Mello ◽

Carlos Roberto Colombo Robazza ◽

João Humberto Antoniazzi

Keyword(s):

Laser Irradiation ◽

Root Canal ◽

Er:Yag Laser ◽

Close Contact ◽

Smear Layer ◽

Canal System ◽

Layer Removal ◽

Sealing Material ◽

Ah Plus ◽

Root Canal System

The sealing of the root canal system is of fundamental importance for successful endodontic treatment. To obtain an adequate apical seal, many factors must be considered such as the presence of smear layer and the sealer applied. After canal preparation, this layer must be removed because it prevents close contact between the dentinal walls and the sealing material. The goal of this study was to evaluate the sealing ability of four different sealers after smear layer removal with either 17% EDTA-T irrigation or Er:YAG laser irradiation of 46 teeth. The canals were sealed with four different sealers: Sealapex, Ketac Endo, AH Plus and N-Rickert. The method for smear layer removal did not influence apical sealing. AH Plus and N-Rickert allowed less dye leakage when compared to Sealapex and Ketac Endo.

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EFFECT OF CHITOSAN GEL ON SMEAR LAYER,Ca/P RATIO AND PUSH-OUT BOND STRENGTH OF RESIN SEALERS AT DIFFERENT TIME INTERVALS.

10.36106/ijsr/0415715 ◽

2021 ◽

pp. 41-44

Author(s):

Niveditha S ◽

Veena S Pai ◽

Roopa R Nadig

Keyword(s):

Bond Strength ◽

Root Resorption ◽

Chitosan Solution ◽

Smear Layer ◽

Time Intervals ◽

Layer Removal ◽

Ah Plus ◽

Open Apices ◽

Push Out ◽

Chitosan Gel

OBJECTIVE: Comparative evaluation of smear layer removal, calcium phosphate ratio and bond strength of AH Plus sealer to radicular dentin after the use of 0.2% chitosan and 17% EDTAas lubricant and nal rinse at different time intervals(1min and 2min). METHODOLOGY: 75 premolars were prepared using Pro-Taper rotary le. 30 samples were used for testing smear layer removal and Ca/Pratio and 45 samples for testing bond strength. 1ml of 3%NaOCl was used for irrigation of all the samples throughout the preparation. The teeth were randomly divided into 3 groups according to the type of lubricant-17% EDTAgel or 0.2% chitosan gel and the nal irrigant -17%EDTA solutions, 0.2%chitosan solution for 1min and 2min. 30 samples were sectioned longitudinally. One half of the section was used for smear layer analysis using SEM and the other half of the section was used for Ca/Pratio analysis using EDX. 45 samples were obturated with F3 gutta-percha cones using AH plus sealer and sectioned. The samples were subjected to push-out testing using UTM and failure was evaluated using SEM. RESULTS: Smear layer removal and push-out bond strength was found to be highest in EDTA group followed by Chitosan(2min) and Chitosan(1min)groups. EDX analysis showed that the Ca/Pratio of EDTAgroup is signicantly lower than chitosan group. CONCLUSION:Within the limitations of the study it can be inferred that 0.2%chitosan gel lubricant and nal rinse can be used as an alternative to EDTAespecially in teeth with thin dentinal walls, open apices, and root resorption.

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Smear layer dissolution by peracetic acid of low concentration

International Endodontic Journal ◽

10.1111/j.1365-2591.2010.01847.x ◽

2011 ◽

Vol 44 (6) ◽

pp. 485-490 ◽

Cited By ~ 25

Author(s):

G. De-Deus ◽

E. M. Souza ◽

J. R. Marins ◽

C. Reis ◽

S. Paciornik ◽

...

Keyword(s):

Peracetic Acid ◽

Smear Layer ◽

Low Concentration

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Evaluation of 0.5% peracetic acid and 2.5% sodium hypochlorite on smear layer removal of root canal instrumented by three rotary systems

Brazilian Dental Science ◽

10.14295/bds.2014.v17i1.959 ◽

2014 ◽

Vol 17 (1) ◽

pp. 62

Author(s):

Ana Paula Martins Gomes ◽

Lais Dos Santos Lissi ◽

Marcella Batista Pavanello Coelho ◽

Eduardo Galera Da Silva ◽

Frederico Canato Martinho ◽

...

Keyword(s):

Acid Solution ◽

Sodium Hypochlorite ◽

Peracetic Acid ◽

Root Canal ◽

Smear Layer ◽

Sodium Hypochlorite Solution ◽

Font Size ◽

Hypochlorite Solution ◽

Layer Removal ◽

Root Canal Irrigants

Objective: The aim of this study was to evaluate the effect of 0.5% peracetic acid solution and 2.5% sodium hypochlorite solution on smear layer removal of root canal walls after rotary instrumentation. Material and methods: Sixty single-rooted human teeth with standardized length had their canals instrumented by three rotary systems (Biorace, MTwo and Endowave), varying the irrigation solution, as follows (n=10): G1- Biorace System + 0.5% peracetic acid solution; G2- Biorace System + 2.5% sodium hypochlorite solution; G3- MTwo System + 0.5% peracetic acid solution; G4- MTwo System + 2.5% sodium hypochlorite solution; G5- Endowave System + 0.5% peracetic acid solution; G6- Endowave System + 2.5% sodium hypochlorite solution. After instrumentation, the roots were cleaved and the dentin walls were evaluated by SEM at x500 and x2000 magnification for assessing the cleaning of the root canals at the cervical, middle and apical thirds. The analyzed area was quantified according to the total number of tubules present and the percentage of open tubules at each region. Data were submitted to statistical analysis by ANOVA and Tukey tests, at the significance level of 5%. Results: There were no statistical significant differences among the experimental groups. In all groups the smear layer removal at cervical and medium thirds was higher than that of apical third. Conclusion: It was concluded that the cleaning obtained at the apical third was lower in all groups studiedand neither the instrumentation technique nor the irrigating solution was able to promote total smear layer removal from root canal walls. KEYWORDS: Peracetic acid; Root canal irrigants; Scanning electron microscopy; Sodium hypochlorite.

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