scholarly journals Structure and distribution of glandular trichomes in three species of Bignoniaceae

2015 ◽  
Vol 45 (4) ◽  
pp. 347-354 ◽  
Author(s):  
Fabíola Fernandes Paiva de Castro FRÓES ◽  
Thália do Socorro Serra GAMA ◽  
Ana Carla FEIO ◽  
Diego DEMARCO ◽  
Ana Cristina Andrade de AGUIAR-DIAS

Glandular trichomes play a major role in the morphological characterization of the Bignoniaceae. Due to their great diversity of forms and functions, this study aimed to inventory the glandular trichomes present in the aerial vegetative axis of Amphilophium magnoliifolium, Martinella obovata and Stizophyllum riparium, analyze their structure and register the participation of ants in these plants. Fresh samples from the nodal region, petiole and from medium to apical regions of the leaflet blade were fixed and processed according to usual methods in light and scanning electron microscopies. The glandular trichomes found were: peltate, capitate, stipitate, and patelliform/cupular. Peltate trichomes are the most abundant ones and present the most uniform distribution. Patelliform/cupular trichomes occur at specific regions, such as prophylls, leaflet blade and nodal regions. Martinella obovata is the only species that presents capitate and stipitate trichomes, which are widely distributed along the entire aerial vegetative axis. Ants were found in all species, mainly at nodal regions. The occurrence of the capitate-type trichome is reported for the first time to the genus.

2017 ◽  
Vol 23 (3) ◽  
pp. 679-686 ◽  
Author(s):  
Milan Gavrilović ◽  
Suzana Erić ◽  
Petar D. Marin ◽  
Núria Garcia-Jacas ◽  
Alfonso Susanna ◽  
...  

AbstractIn this work, weddellite and sylvite crystals are identified for the first time on the involucral bracts and petals of Xeranthemum annuum and Xeranthemum cylindraceum using scanning electron microscopy coupled with energy dispersive spectrometric (SEM-EDS) analysis. Well-developed crystals of weddellite (CaC2O4·2H2O) occur in the form of a tetragonal bipyramid (hhl), rarely in combination of a bipyramid and tetragonal prism (h00). Indumentum of involucral bracts of X. cylindraceum consists of nonglandular and glandular trichomes. Sylvite (KCl) crystals are observed only on the petal surface of X. cylindraceum. The crystals of sylvite occur in the form of perfect cubes (hexahedrons), but some crystals are deformed, i.e., partially elongated. Taxonomic significance of investigated microcharacters as well as the use of SEM-EDS analysis in taxonomic studies of plants are discussed.


2016 ◽  
Vol 34 (2) ◽  
pp. 412-417
Author(s):  
Esra Öztürk

AbstractIn this work, aluminate type phosphorescence materials were synthesized via the solid state reaction method and the photoluminescence (PL) properties, including excitation and emission bands, were investigated considering the effect of trace amounts of activator (Eu3+) and co-activator (Dy3+). The estimated thermal behavior of the samples at certain temperatures (> 1000 °C) during heat treatment was characterized by differential thermal analysis (DTA) and thermogravimetry (TG). The possible phase formation was characterized by X-ray diffraction (XRD). The morphological characterization of the samples was performed by scanning electron microscopy (SEM). The PL analysis of three samples showed maximum emission bands at around 610 nm, and additionally near 589 nm, 648 nm and 695 nm. The bands were attributed to typical transitions of the Eu3+ ions.


2012 ◽  
Vol 40 (1) ◽  
pp. 35 ◽  
Author(s):  
Hüseyin CILDIR ◽  
Ahmet KAHRAMAN ◽  
Musa DOGAN

In this study, the epidermal types and their distribution on dorsal and lateral petals, the trichome types and their density on sepals of Lathyrus chloranthus, L. digitatus, L. laxiflorus subsp. laxiflorus, L. roseus subsp. roseus, L. sativus, and L. tuberosus, belonging to sections Lathyrus, Lathyrostylis, Pratensis, and Orobon of the genus Lathyrus in Turkey were investigated using light microscopy (LM) and scanning electron microscopy (SEM), and the systematic significance of these characters was evaluated. These taxa, except L. sativus, are studied for the first time under aspects of the petal and sepal micromorphology. Three major epidermal types were recognized on the petal: tabular rugose striate cells (TRS), areolate cells with more or less striations (AS), and papillose conical striate cells (PCS). TRS and AS were further subdivided into three subtypes. TRS was found on the dorsal and lateral petals of L. chloranthus, L. sativus, and L. tuberosus: the dorsal petals of L. roseus subsp. roseus and the lateral petals of L. laxiflorus subsp. laxiflorus, while AS was present on the dorsal and lateral petals of L. digitatus and the lateral petals of L. roseus subsp. roseus. PCS was found only on the dorsal petals of L. digitatus. Three main types of trichomes on the sepal were observed: peltate glandular, capitate glandular, and nonglandular trichomes. The capitate glandular and nonglandular trichomes were further subdivided into three subtypes. The peltate glandular trichomes were present only in L. chloranthus, but absent in the others. The capitate glandular trichomes were found in L. chloranthus, L. laxiflorus subsp. laxiflorus, L. roseus subsp. roseus, and L. tuberosus. The nonglandular trichomes were always present in L. chloranthus and L. laxiflorus subsp. laxiflorus. The present results show that the petal and sepal micromorphology can be used in delimitation of the taxa based on petal and sepal micromorphology.


2015 ◽  
Vol 1109 ◽  
pp. 381-384
Author(s):  
M. Safwan Azmi ◽  
Sharipah Nadzirah ◽  
Uda Hashim

The purpose of this paper is to study the morphological characterization of aluminum interdigitated electrodes (IDE) of different gap sizes on silicon substrate. The electrodes were fabricated using standard photolithography process and were done so with sizes of 12 μm, 10 μm and 7 μm. The electrodes were morphologically characterized using scanning electron microscope (SEM) and high-powered microscope (HPM).Keywords: morphological, interdigitated electrodes, aluminum


Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 731
Author(s):  
Anahí G. Díaz ◽  
Paula G. Ragone ◽  
Fanny Rusman ◽  
Noelia Floridia-Yapur ◽  
Rubén M. Barquez ◽  
...  

Trypanosomes are a group of parasitic flagellates with medical and veterinary importance. Despite many species having been described in this genus, little is known about many of them. Here, we report a genetic and morphological characterization of trypanosomatids isolated from wild mammals from the Argentine Chaco region. Parasites were morphologically and ultrastructurally characterized by light microscopy and transmission electron microscopy. Additionally, 18s rRNA and gGAPDH genes were sequenced and analyzed using maximum likelihood and Bayesian inference. Morphological characterization showed clear characteristics associated with the Trypanosoma genus. The genetic characterization demonstrates that the studied isolates have identical sequences and a pairwise identity of 99% with Trypanosoma lainsoni, which belongs to the clade of lizards and snakes/rodents and marsupials. To date, this species had only been found in the Amazon region. Our finding represents the second report of T. lainsoni and the first record for the Chaco region. Furthermore, we ultrastructurally described for the first time the species. Finally, the host range of T. lainsoni was expanded (Leopardus geoffroyi, Carenivora, Felidae; and Calomys sp., Rodentia, Cricetidae), showing a wide host range for this species.


2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
A. Hedayat ◽  
J. Szpunar ◽  
N. A. P. Kiran Kumar ◽  
R. Peace ◽  
H. Elmoselhi ◽  
...  

Background. Morphological characterization of hemodialysis membranes is necessary to improve pore design.Aim. To delineate membrane pore structure of a high flux filter, Polyflux 210H.Methods. We used a Joel JSM-6010LV scanning electron microscope (SEM) and a SU6600 Hitachi field emission scanning electron microscope (FESEM) to characterize the pore and fiber morphology. The maximal diameters of selected uremic toxins were calculated using the macromolecular modeling Crystallographic Object-Oriented Toolkit (COOT) software.Results. The mean pore densities on the outermost and innermost surfaces of the membrane were 36.81% and 5.45%, respectively. The membrane exhibited a tortuous structure with poor connection between the inner and outer pores. The aperture’s width in the inner surface ranged between 34 and 45 nm, which is 8.76–11.60 times larger than the estimated maximum diameter ofβ2-microglobulin (3.88 nm).Conclusion. The results suggest that the diameter size of inner pore apertures is not a limiting factor to middle molecules clearance, the extremely diminished density is. Increasing inner pore density and improving channel structure are strategies to improve clearance of middle molecules.


2019 ◽  
Vol 125 (3) ◽  
pp. 521-532
Author(s):  
Emma Sarath ◽  
Kazune Ezaki ◽  
Takenori Sasaki ◽  
Yu Maekawa ◽  
Yuji Sawada ◽  
...  

Abstract Background and aims Domatia are plant structures within which organisms reside. Callicarpa saccata (Lamiaceae) is the sole myrmecophyte, or ‘ant plant’, that develops foliar (leaf-borne) myrmeco-domatia in this genus. In this work we examined domatium development in C. saccata to understand the developmental processes behind pouch-like domatia. Methods Scanning electron microscopy, sectioning and microcomputed tomography were carried out to compare the leaves of C. saccata with those of the closely related but domatia-less myrmecophyte Callicarpa subaequalis, both under cultivation without ants. Key results Callicarpa saccata domatia are formed as a result of excess cell proliferation at the blade/petiole junctions of leaf primordia. Blade/petiole junctions are important meristematic sites in simple leaf organogenesis. We also found that the mesophyll tissue of domatia does not clearly differentiate into palisade and spongy layers. Conclusions Rather than curling of the leaf margins, a perturbation of the normal functioning of the blade/petiole junction results in the formation of domatium tissue. Excess cell proliferation warps the shape of the blade and disturbs the development of the proximal–distal axis. This process leads to the generation of distinct structures that facilitate interaction between C. saccata and ants.


Zootaxa ◽  
2011 ◽  
Vol 2906 (1) ◽  
pp. 52
Author(s):  
XIAOQIANG LI ◽  
BINGZHONG REN ◽  
YUTING ZOU ◽  
JIAN ZHANG ◽  
YINLIANG WANG

The present study compares the proventricular morphology, analyzed under optic microscope and scanning electron microscopy (SEM), among ten Grylloidea species. The result showed that the size of proventriculus was of critical value. Internally, the main differences were the number of sclerotized appendix (sa), middle denticles (md) and lateral denticles (ld), and the structure of lateral teeth (lt). In addition, we analyzed the crickets’ feeding habits and note that the the proventriculus possesses highly sclerotized projections which act in the selection of victuals. The morphology of proventriculus is closely related to feeding habits. A clustering analysis of seven features of the proventriculus was constructed. It revealed that the proventriculus had significance for taxonomy and species relationships. Observations on morphological characterization of proventricular morphology will be useful in future studies of the feeding habits and phylogeny of crickets.


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