scholarly journals Transfer of passive immunity and serum proteinogram in the first six months of life of Criollo Lageano and black and white holstein calves

2012 ◽  
Vol 32 (10) ◽  
pp. 980-986 ◽  
Author(s):  
William Timboni Teixeira ◽  
Graziela Vieira Fonteque ◽  
Alexandre Floriani Ramos ◽  
Arthur da Silva Mariante ◽  
Andrea Alves do Egito ◽  
...  
Keyword(s):  
Zinc Sulfate ◽  
Serum Proteins ◽  
Passive Immunity ◽  
Total Serum Protein ◽  
Total Serum ◽  
Gamma Glutamyl Transferase ◽  
Significance Level ◽  
Black And White ◽  
Holstein Calves ◽  
Glutamyl Transferase

The objective of this study was to evaluate and compare the transfer of passive immunity and the proteinogram in Criollo Lageano (CL) and Black and White Holstein (BWH) calves. Two groups were utilized with 13 Criollo Lageano and 10 BWH calves. Blood samples were collected for the measurement of total serum protein, electrophoresis of serum proteins, activity of the gamma glutamyl transferase, and concentration of IgG by the method of the zinc sulfate turbidity in periods between 24 and 36 hours of life, 15, 30, 60, 90, 120, 150 and 180 days. Statistical analysis was performed by ANOVA and Tukey test at 5% significance level, and correlations between variables were calculated. Variations of serum proteins followed a pattern of physiological behavior over the first six months of life and production of immunoglobulins was active earlier in BWH calves and slower in the Criollo Lageano, without causing any impact on their health. Gamma globulin in the first days of life (24-36h) was correlated with IgG (r=0.87 for CL and r=0.89 for BWH), PTS (r=0.91 for CL and r=0.92 for BWH), Glob (r=0.99 for CL and r=0.98 for BWH) and GGT (r=0.14 for CL and r=0.83 for BWH). It was concluded that there was no failure in the transfer of passive immunity in Criollo Lageano calves but this failure occurred in the BWH calves. IgG values estimated by the zinc sulfate turbidity and serum proteins were considered good indicators of the transfer of passive immunity in calves between 24 and 36 hours of life.

Muscle Weight Relationship to Serum Proteins, Hemocytes, and Hepatopancreas in the Lobster, Homarus americanus

1967 ◽  
Vol 24 (11) ◽  
pp. 2339-2354 ◽  
Author(s):  
James E. Stewart ◽  
John W. Cornick ◽  
Diane M. Foley ◽  
M. F. Li ◽  
C. M. Bishop
Keyword(s):  
Serum Protein ◽  
Physiological Condition ◽  
Serum Proteins ◽  
Homarus Americanus ◽  
Total Serum Protein ◽  
Total Serum ◽  
Experimental Conditions ◽  
Muscle Weight ◽  
Beef Liver ◽  
The Relationship

Total serum protein values, hemocyte numbers, and muscle weights were determined for 216 intermolt lobsters immediately after their capture, and for 230 others held captive under a variety of dietary and environmental conditions. Average muscle values ranged from approximately 13% to the more normal 20–25% of the live animals' weight, depending upon experimental conditions. The total serum protein up to a level of 55 mg/ml was shown to be a reliable indicator of muscle weights, although the relationship was not identical for all lobster groups. It appeared to be modified chiefly by the areas from which the different groups were taken. Diet was more important than the temperatures (5 to 14 C) in affecting changes in muscle and serum protein values. Starvation caused a greater reduction (50 to 70%) in the size of the hepatopancreas than in the muscle. Histological examination of the hepatopancreatic tissue showed that the lipid content was markedly reduced upon starvation and that a degeneration of this organ was apparent for lobsters fed a beef liver and herring diet. Measurement of serum proteins would appear to be a useful technique in experiments on lobster nutrition and have value, within specified limits, for assessing the physiological condition of wild lobsters.

67 Association of Morbidity and Failure of Transfer of Passive Immunity in Beef-dairy Crossbred Calves

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 39-39
Author(s):  
Jessica Pereira ◽  
Marcos I Marcondes ◽  
Pedro H Carvalho ◽  
Noelia Silva Del Rio ◽  
João Bittar ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Cohort Study ◽  
Survival Analysis ◽  
Respiratory Disease ◽  
Strong Correlation ◽  
Central Valley ◽  
Descriptive Statistics ◽  
Passive Immunity ◽  
Total Serum Protein ◽  
Total Serum

Abstract The objective of this prospective cohort study was to describe the prevalence of failure of transfer of passive immunity (FTPI) in beef-dairy crossbred calves, and its association with morbidity up to 30 d of life. A total of 393 one d old calves were enrolled upon arrival at a calf facility in Central Valley, California, from Jan-Mar 2021. Farm of origin, breed, sex, and weight were recorded at the day of arrival. Blood was collected at 24 ± 1 h post-arrival to evaluate total serum protein (TSP) and % Brix reading. Calves were classified as having FTPI if TSP < 5.2 g/dL, and brix < 8.2%. Farm personnel performed daily health evaluations and recorded diseases. Descriptive statistics, logistic regression, and survival analysis were conducted using SAS. Calves’ breeds were Angus × Holstein (AN × HO, 58%), Charolais × Jersey (CH × JE, 37%), and Angus × Jersey (AN × JE, 5%). Prevalence of FTPI was 36% (TSP) and 34% (brix). TSP and %Brix readings had a strong correlation (r = 0,99, P < 0.0001). Overall morbidity was 78% (70% had only diarrhea, 7% only respiratory disease and 23% both). The odds of disease up to 30 d was not associated with sex (female vs. male, OR = 0.77, 95% CI = 0.36–1.62), or breed (AN × HO vs. CH × JE, OR = 1.35, CI = 0.63–2.91; AN × JE not included in this analysis). FTPI was associated with greater odds of disease (OR = 2.91, CI = 1.19–7.09, P = 0.01). There was a tendency for association between FTPI and the hazard of disease up to 30 d (HR = 1.43, CI = 0.96–2.10, P = 0.07). Therefore, FTPI may predispose beef-dairy crossbred calves to diarrhea and respiratory disease up to 30 d of life.

scholarly journals Serum proteinogram of the Campeiro horse

2019 ◽  
Vol 71 (2) ◽  
pp. 363-368
Author(s):  
A.F. Souza ◽  
J. Schade ◽  
A.F. Ramos ◽  
M.S.M. Albuquerque ◽  
G.V. Fonteque ◽  
...  
Keyword(s):  
Serum Protein ◽  
Serum Proteins ◽  
Reference Interval ◽  
Total Serum Protein ◽  
Total Serum ◽  
Serum Concentrations ◽  
Reproductive Condition ◽  
Serum Fractions ◽  
Pregnancy Status ◽  
Biuret Method

ABSTRACT The aim of this study to measure the fractions of the total serum proteins of the Campeiro horse and identify the influences of biological variants. Blood samples were taken in 138 horses of the breed Campeiro for measuring the concentration of total serum protein by the biuret method. Serum concentrations of protein fractions were measured by electrophoresis using agarose gel. Groups were formed according to age, sex and reproductive condition. The average values of serum fractions: albumin (2.85±0.36g/dl), alpha 1 (0.28±0.11g/dl), alpha 2 (0.26±0.08g/dL) beta 1 (0.57±0.15g/dl), beta 2 (0.89±0.28g/dL), gamaglobulinas (1.86±0.34g/dL), albumin/globulin ratio (0.75±0.18) and 2.5% percentile and 97.5% had slight differences in relation to the reference interval proposed for the species. They observed higher values of alpha 1 and 2 globulins in the group from that had six to eight years old and gammaglobulins in group above 13 years old. Serum protein concentrations were similar in horses and mares and between non-pregnant and pregnant. Sex and pregnancy status did not affect serum proteinogram. Alpha and gammaglobulins have higher values as the age increases. Serum proteinogram of Campeiro horses shows variations that have to be considered in the interpretation of laboratory tests.

Precipitation of serum proteins by the lectin from wheat-germ.

1987 ◽  
Vol 33 (1) ◽  
pp. 185-186 ◽  
Author(s):  
W E Schreiber ◽  
L Whitta
Keyword(s):  
Alkaline Phosphatase ◽  
Serum Protein ◽  
Wheat Germ ◽  
Serum Proteins ◽  
Total Serum Protein ◽  
Total Serum ◽  
Wheat Germ Lectin

Abstract We investigated the composition of the precipitate that forms when wheat-germ lectin derived from Triticum vulgaris is added to serum. A number of serum proteins are precipitated, representing about 2.5% of the total serum protein. This study demonstrates that the interaction of this lectin with the bone isoenzyme of alkaline phosphatase is not specific.

scholarly journals The Toxic Effect of Pb Nanoparticles Prepared by Laser ablation on Some Biochemical Aspects in Rats

2021 ◽  
Vol 19 (48) ◽  
pp. 107-114
Author(s):  
Fiham Jasim Alobaidi ◽  
Abid A. Thaker ◽  
Asmiet Ramizy
Keyword(s):  
Laser Ablation ◽  
Characteristic Feature ◽  
Toxic Effect ◽  
Serum Proteins ◽  
Spherical Shape ◽  
Average Diameter ◽  
Total Serum Protein ◽  
Total Serum ◽  
Pure Lead ◽  
Experimental Animals

This work aimed to prepare and study the characteristic feature of lead nanoparticles (PbNPS) and follow its effects on some physiological aspects in rats.PbNPS was prepared by laser ablation of pure lead mass with a pulse of 500 and 100 mJ of energy. The results indicated that the wavelength was approximately 196 and the concentration was reported at 53,8967 mg / L. AFM, as the average diameter has been estimated at 69.93 nm. EFSEM shows the spherical shape of the particle.The experimental animals (rats) were divided into two groups, with seven rats for each one. The first group was a control and the second group was injected with 1 milliliter of PbNPS (53.8673 mg/l) per day for 45 days. Bioaccumulated lead ( in liver, spleen kidney and muscles), total serum proteins, albumin, ALT, AST, ALP and antioxidants (GSH, SOD, CAT, and MDA) were estimated.By comparison to the control, PbNPS exposed animals. There were no significant differences in the bioaccumulated lead. In contrast, there was a significant reduction in total serum protein, albumin, GSH, SOD, CAT. Also, there was an increase in serum ALT, AST, ALP and MDA.

LEVELS AND PARTITION OF THE FOWL'S SERUM PROTEINS IN RELATION TO AGE AND EGG PRODUCTION

1955 ◽  
Vol 33 (1) ◽  
pp. 891-903 ◽  
Author(s):  
W. E. Vanstone ◽  
W. A. Maw ◽  
R. H. Common
Keyword(s):  
Serum Protein ◽  
Protein Level ◽  
Egg Production ◽  
Serum Proteins ◽  
Electrophoretic Pattern ◽  
Total Serum Protein ◽  
Total Serum ◽  
Zone Electrophoresis ◽  
Serum Protein Level ◽  
New Protein

The level of total serum protein in the fowl has been followed from the 14th day of incubation to the 13th week of egg production. Serum proteins have been fractionated concurrently by zone electrophoresis in filter paper. Serum protein level in the 14-day embryo was 0.9 gm./100 ml. and the protein comprised a prealbumin fraction, albumin, α2- and β-globnlins. Sera from chicks aged seven days no longer contained a prealbumin fraction but α1-,α3- and γ-globulin fractions had appeared by this stage and the protein level had reached 2.3 gm./100 ml. Total serum protein in females increased to a maximum of about 5.4 gm./100 ml. in the week before laying of the first egg. By that time two new protein fractions had appeared. These new fractions accounted for the greater part of the increase over the levels (4.0 gm./100 ml.) prevailing in the prepuberal stage. Serum protein level declined during the first three weeks of laying to average levels below 4.0 gm./100 ml.; and this decline appeared to affect albumin and α1-globulin as well as the new fractions. As laying progressed, the total serum protein tended to regain a level around 4.0 gm./100 ml. The electrophoretic pattern also tended to return towards that prevailing in the week before laying. Some tentative correlations of the results of zone electrophoresis with published results for free electrophoresis of fowl serum proteins are presented.

scholarly journals Dietary supplementation of Ascophylum nodosum improved kidney function of mink challenged with Aleutian mink disease virus

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
A. Hossain Farid ◽  
Nancy J. Smith
Keyword(s):  
Immune Response ◽  
Kidney Function ◽  
Disease Virus ◽  
Serum Protein ◽  
Virus Replication ◽  
Antibody Titer ◽  
Total Serum Protein ◽  
Total Serum ◽  
Aleutian Mink Disease Virus ◽  
Glutamyl Transferase

Abstract Background Feed additives which can ease the negative effects of infection by the Aleutian mink disease virus (AMDV) are of interest to mink farmers. The effects of kelp meal (Ascophylum nodosum) supplementation on immune response, virus replication and blood parameters of mink inoculated with AMDV were assessed. AMDV-free black mink (n = 75) were intranasally inoculated with a local strain of AMDV and fed a commercial pellet supplemented with kelp meal at the rates of 1.5% or 0.75% of the feed or were kept as controls (no kelp) for 451 days. Blood was collected on days 0 (pre-inoculation), 31, 56, 99, 155, 366 and 451 post-inoculation (dpi). Results No significant difference was observed among the treatments for the proportion of animals positive for antibodies against the virus measured by the counter-immunoelectrophoresis (CIEP), viremia measured by PCR, antibody titer measured by quantitative ELISA, total serum protein measured by a refractometer or elevated levels of gamma globulin measured by iodine agglutination test at the sampling occasions. At the termination of the experiment on 451 dpi, there were no differences among treatments for antibody titer measured by CIEP, total serum protein, albumin, globulins, albumin:globulin ratio, alkaline phosphatase, gamma-glutamyl transferase, and proportions of PCR positive spleen, lymph node or bone marrow samples, but blood urea nitrogen and creatine levels were significantly lower in the 1.5% kelp supplemented group than in the controls. Conclusion Kelp supplementation improved kidney function of mink infected with AMDV with no effect on liver function, immune response to infection by AMDV or virus replication.

Studies on the hygiene of drinking water for laboratory animals. 2. Clinical and biochemical studies in rats and rabbits during long-term provision of acidified drinking water

1981 ◽  
Vol 15 (2) ◽  
pp. 111-117 ◽  
Author(s):  
B. K. Tober-Meyer ◽  
H. J. Bieniek ◽  
I. R. Kupke
Keyword(s):  
Drinking Water ◽  
Growth Curves ◽  
Laboratory Animals ◽  
Total Serum Protein ◽  
Total Serum ◽  
Arterial Blood ◽  
Water Ph ◽  
Glutamyl Transferase ◽  
Serum Glutamate

The reaction of rats and rabbits to long-term application of acidified drinking water (pH 2·3-2·5) was observed over a 7-months period. The following parameters were studied: growth curves initiated at weaning, haematology, blood glucose, total serum protein, creatinine, inorganic phosphate, sodium, potassium, calcium, alkaline phosphatase, creatinine kinase, serum glutamate oxalacetate transaminase, and serum glutamate pyruvate transaminase in serum as well as the acid-base status in arterial blood; in addition in rabbits γ-glutamyl transferase and lactate dehydrogenase were examined in the serum. No significant changes were seen in comparison to the control groups.

scholarly journals Mastitis in the transition period: identification of potential blood markers

2018 ◽  
Vol 70 (4) ◽  
pp. 1120-1128 ◽  
Author(s):  
K.R. Santos ◽  
F.N. Souza ◽  
M.G. Blagitz ◽  
C.F. Batista ◽  
H.G. Bertagnon ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Somatic Cell ◽  
Cell Count ◽  
Somatic Cell Count ◽  
Serum Proteins ◽  
Transition Period ◽  
Total Serum Protein ◽  
Total Serum ◽  
Fibrinogen Concentration ◽  
Milk Samples

ABSTRACT This study identified potential blood markers associated with mastitis in dairy cows under different dry therapies during the transition period, using a logistic regression model. Thirty-four Holstein dairy cows were divided into three groups: untreated controls (13 cows, 42 quarters); animals that received an antimicrobial at drying-off (10 cows, 40 quarters); and animals that were administered an internal teat sealant at drying-off (11 cow, 44 quarters). Blood and quarter milk samples were collected 60 days before the expected day of calving, on the day of calving, and three, seven, 15, 21, and 30 days after calving. Milk samples were submitted for bacteriological analysis and somatic cell count. Blood samples were collected for analyses of the following: the erythrogram and leucogram; plasm fibrinogen concentration; hepatic and renal functions; metabolic profile; serum calcium and phosphorous levels; total serum protein and albumin concentrations. The concentration of total serum proteins was associated with a high somatic cell count. Similarly, the concentrations of total serum proteins and triglycerides were associated to milk bacteriological positive samples during the transition period. Thus, the occurrence of mastitis in dairy cows during the transition period was greater in animals that showed higher concentrations of serum total proteins and triglycerides, in contrast to the use of dry cow therapy.

LEVELS AND PARTITION OF THE FOWL'S SERUM PROTEINS IN RELATION TO AGE AND EGG PRODUCTION

1955 ◽  
Vol 33 (6) ◽  
pp. 891-903 ◽  
Author(s):  
W. E. Vanstone ◽  
W. A. Maw ◽  
R. H. Common
Keyword(s):  
Serum Protein ◽  
Protein Level ◽  
Egg Production ◽  
Serum Proteins ◽  
Electrophoretic Pattern ◽  
Total Serum Protein ◽  
Total Serum ◽  
Zone Electrophoresis ◽  
Serum Protein Level ◽  
New Protein

The level of total serum protein in the fowl has been followed from the 14th day of incubation to the 13th week of egg production. Serum proteins have been fractionated concurrently by zone electrophoresis in filter paper. Serum protein level in the 14-day embryo was 0.9 gm./100 ml. and the protein comprised a prealbumin fraction, albumin, α2- and β-globnlins. Sera from chicks aged seven days no longer contained a prealbumin fraction but α1-,α3- and γ-globulin fractions had appeared by this stage and the protein level had reached 2.3 gm./100 ml. Total serum protein in females increased to a maximum of about 5.4 gm./100 ml. in the week before laying of the first egg. By that time two new protein fractions had appeared. These new fractions accounted for the greater part of the increase over the levels (4.0 gm./100 ml.) prevailing in the prepuberal stage. Serum protein level declined during the first three weeks of laying to average levels below 4.0 gm./100 ml.; and this decline appeared to affect albumin and α1-globulin as well as the new fractions. As laying progressed, the total serum protein tended to regain a level around 4.0 gm./100 ml. The electrophoretic pattern also tended to return towards that prevailing in the week before laying. Some tentative correlations of the results of zone electrophoresis with published results for free electrophoresis of fowl serum proteins are presented.

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