scholarly journals Hand-sewn and stapled esophageal anastomosis: experimental study in dogs

2004 ◽  
Vol 19 (4) ◽  
pp. 319-327 ◽  
Author(s):  
Cervantes Caporossi ◽  
Ivan Cecconello ◽  
José Eduardo Aguilar-Nascimento ◽  
Filadelfio Venço ◽  
Joaquim José Gama-Rodrigues
Keyword(s):  
Experimental Study ◽  
Bursting Pressure ◽  
Esophageal Anastomosis ◽  
Pressure Test ◽  
Stapler Anastomoses ◽  
Mechanical Suture ◽  
Group 2 ◽  
Intention Group ◽  
Group 1

PURPOSE: To compare experimentally, the healing of cervical oesophageal anastomoses performed either with stapler or 2-layer hand-sewn sutures. METHODS: Thirty six dogs were randomised to two groups: stapled anastomoses (n = 18); hand-sewn anastomoses (n = 18). Each group was divided into three subgroups, corresponding to the day of sacrifice (3rd, 7th and 14th postoperative day). Healing was assessed by: a) anatopathology b) anastomotic resistance to bursting pressure test; c) quantification of hidroxyproline RESULTS: Group 1 heal by second intention, group 2 showed a healing by first intention. Bursting pressure was similar between groups at day 3, though group 1 animals showed it significantly higher at day 7and day 14 compared with group 2. Statiscally, there were no interaction between the day of sacrifice and groups, as well as there was no difference among the dates of observation regarding the results of hidroxyproline CONCLUSIONS: a) mechanical suture is more resistant than hand-sewn; b) In stapler anastomoses, healing was as secondary union, whereas in hand-sewn anastomoses, healing was by first intention; c) no correlation was found in the results of bursting pressure and hidroxyproline quantification.

scholarly journals Histomorphological evaluation of osteoclast cell count in femur bone of mice induced by anastrazole and protective effect of olive oil.

2021 ◽  
Vol 28 (12) ◽  
pp. 1837-1843
Author(s):  
Anjum Ishaque ◽  
Saima Nadeem ◽  
Shagufta Nisar ◽  
Hasnain Ali Shah ◽  
Khalid Javed ◽  
...  
Keyword(s):  
Experimental Study ◽  
Bone Loss ◽  
Olive Oil ◽  
Cell Count ◽  
Control Group ◽  
Femur Bone ◽  
Group 3 ◽  
Albino Mice ◽  
Group 2 ◽  
Group 1

Objective: The objective of this study is to find out protective effect of olive oil to prevent bone loss by decreasing osteoclast count in patient receiving Anastrazole. Study Design: Experimental study. Setting: Pakistan Council for Scientific and Industrial Research (PCSIR) Animal House, Peshawar and Pathology Lab KGMC Peshawar. Period: March 2019 to December 2019. Material & Methods: Sixty female albino mice 6-8 weeks of age were selected for this experimental study and Aromatase inhibitor drug Anastrazole was given alone and in combination with olive oil once daily for 30 successive days. Femur bone samples were collected and stained with Eosin and Hematoxylin for histomorphological evaluation of osteoclast cell count in three all three groups i.e. control group, those receiving Anastrazole alone and those given Anastrazole and olive oil in combination. Results: The mean weight of all experimental female albino mice before study was 30.77- 33.05 grams and after the study was 30.84- 21.31 grams. Control group 1 which was given normal diet showed increased weight of mice with less osteoclast cell count as compared to experimental groups (2 and 3).  In group 2 (Drugged) which was given Anastrazole, weight of were lesser than control group 1 and group 3(Anastrazole + olive oil), while, osteoclast score was greater than group 1(control) and group 3 (Anastrazole + olive oil). Group3 (Drugged+ Olive oil) showed greater weight of mice than group 2 (Anastrazole) but, lesser than control group 1. Osteoclast score was greater than control group but lesser than group 2 (Anastrazole). Conclusion: The results showed positive and protective effects of olive oil against Anastrazole induced bone loss in female albino mice.

scholarly journals Influence of toxoplasma gondii antigens on features of immune status at prenatal and early postnatal periods: experimental study

2014 ◽  
Vol 95 (3) ◽  
pp. 398-401
Author(s):  
T F Sokolova ◽  
D G Novikov ◽  
A V Indutny
Keyword(s):  
Experimental Study ◽  
Toxoplasma Gondii ◽  
Blood Count ◽  
Immune Status ◽  
Perinatal Period ◽  
Female Rats ◽  
Control Group ◽  
White Rats ◽  
Group 2 ◽  
Group 1

Aim. To detect the features of immune status at prenatal and early postnatal periods under the influence of Toxoplasma gondii antigens. Methods. The experimental study was performed on Wistar white rats, who were the offspring of the female rats who were sensitized by T. gondii corpuscular antigen during the III trimester of pregnancy - group 1 (n=96) and on animals who were administered T. gondii corpuscular antigen at firs day of life - group 2 (n=103). Control group consisted of intact rats. Common blood test, levels of antibody-forming cells and CD3+ cells were assessed at 60 day after birth. Results. Neutrophil blood count was 1.9 times higher in the group 1 rats compared to group 2. Eosinophil blood count was 1.4 times lower in the group 1 compared to control group (р=0.01), and 2 times lower in the group 2 compared to control group (р=0.002). At the same time, lymphocyte count was comparable in the group 1 rats and control group, while it was 1.4 times lower in the group 2 compared to control group (р=0.04). Together with that, there was a reduction of CD3+ cells and antibody-forming cells in blood and spleen, which was more marked in the 2nd group. Lymphoadenopathy, thymus dysgenesia, reduced blood and spleen T-cells levels, low humeral immunity were found in Wistar white rats, who were the offspring of the female rats who were sensitized by T. gondii corpuscular antigen during the III trimester of pregnancy. Conclusion. The influence T. gondii corpuscular antigen on rats during perinatal period results in secondary immunodeficiency, persisting at 60 day of life.

scholarly journals Stapling technology with triple-row and enhanced staple configurations evaluated in a series of 124 consecutive laparoscopic vertical gastrectomies

2019 ◽  
Vol 6 (6) ◽  
pp. 1857
Author(s):  
Alessandro Bianchi ◽  
Alberto Pagan-Pomar ◽  
Marina Jimenez-Segovia ◽  
Carla Soldevila-Verdeguer ◽  
Jaume Bonnin-Pascual ◽  
...  
Keyword(s):  
Staple Line ◽  
Suture Line ◽  
Clinical Results ◽  
Anthropometric Parameters ◽  
Surgical Time ◽  
Vertical Gastrectomy ◽  
Gastric Leakage ◽  
Mechanical Suture ◽  
Group 2 ◽  
Group 1

Background: Laparoscopic sleeve gastrectomy (LSG) is a procedure frequently used to treat morbid obesity, due to its simplicity compared to other bariatric techniques. However, LSG can lead to serious complications, such as gastric leakage and bleeding from the staple line. To reduce these complications, seroserosal reinforcement of the mechanical suture line after gastrectomy is generally recommended. In recent decades, studies have focused on the safety of anastomosis with staples, especially compared to manual sewing techniques. Since studies on the improvement of stapling technology are limited, this study arises to compare the clinical results of staple line oversewing versus stapling technology with triple-row and enhanced staple configurations in laparoscopic sleeve gastrectomy.Methods: A retrospective review from a prospectively maintained database of 124 laparoscopic vertical gastrectomies performed at single centre between March 2010 and December 2016. Patients were divided into two groups, with comparable anthropometric parameters and inclusion criteria. Seroserosal reinforcement was used in the first group, and stapling technology with triple-row and enhanced staple configurations in the second. Rates of anastomotic leakage, bleeding, reoperation, and 30-day mortality were compared.Results: In Group 1 the average surgical time was 125 min, whereas in Group 2 it was 87 min. No differences were found between the two groups regarding leakage or bleeding of the staple line.Conclusions: The use of triple-row stapling devices during laparoscopic vertical gastrectomy enables surgical time to be reduced. Further high-quality studies to evaluate the efficacy and effectiveness of stapling technology with triple-row and enhanced staple configurations are needed.

scholarly journals Effect of three different needle holders on gastrointestinal anastomosis construction time and bursting pressure in equine jejunal segments

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Kate Averay ◽  
Gaby van Galen ◽  
Michael Ward ◽  
Denis Verwilghen
Keyword(s):  
Intestinal Resection ◽  
Bursting Pressure ◽  
Construction Time ◽  
Needle Holder ◽  
Group 4 ◽  
Bursting Pressures ◽  
Group 3 ◽  
Group 2 ◽  
The Impact ◽  
Group 1

Abstract Background Equine small intestinal resection and anastomosis is a procedure where optimizing speed, without compromising integrity, is advantageous. There are a range of different needle holders available, but little is published on the impact surgical instrumentation has on surgical technique in veterinary medicine. The objectives of this study were to investigate if the needle holder type influences the anastomosis construction time, the anastomosis bursting pressure and whether the bursting pressure is influenced by the anastomosis construction time. Single layer end-to-end jejunojejunal anastomoses were performed on jejunal segments harvested from equine cadavers. These segments were randomly allocated to four groups. Three groups based on the needle holder type that was used: 16.5 cm Frimand (Group 1), 16 cm Mayo-Hegar (Group 2) or 20.5 cm Mayo-Hegar (Group 3) needle holders. One (Group 4) as control without anastomoses. Anastomosis construction time was recorded. Bursting pressure was determined by pumping green coloured fluid progressively into the lumen whilst recording intraluminal pressures. Maximum pressure reached prior to failure was recorded as bursting pressure. Construction times and bursting pressures were compared between needle holder, and the correlation between bursting pressure and construction time was estimated. Results Construction times were not statistically different between groups (P = 0.784). Segments from Group 2 and Group 3 burst at a statistically significantly lower pressure than those from Group 4; P = 0.031 and P = 0.001 respectively. Group 4 and Group 1 were not different (P = 0.125). The mean bursting pressure was highest in Group 4 (189 ± 61.9 mmHg), followed by Group 1 (166 ± 31 mmHg) and Group 2 (156 ± 42 mmHg), with Group 3 (139 ± 34 mmHg) having the lowest mean bursting pressure. Anastomosis construction time and bursting pressure were not correlated (P = 0.792). Conclusions The tested needle holders had a significant effect on bursting pressure, but not on anastomosis construction time. In an experimental setting, the Frimand needle holder produced anastomoses with higher bursting pressures. Further studies are required to determine clinical implications.

scholarly journals Pengaruh Susu Kacang Kedelai (GLYCINE MAX (L.) MERR.) Terhadap Kualitas Spermatozoa Tikus Wistar (Rattus Norvegicus)

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Valentine Madianung ◽  
Lusiana Satiawati ◽  
Lydia Tendean
Keyword(s):  
Experimental Study ◽  
Amino Acid ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Day Treatment ◽  
Control Group ◽  
Spermatozoa Motility ◽  
Glycine Max L ◽  
Group 2 ◽  
Group 1

Abstract: The effects of soy beans on spermatozoa still been a controversial thing. Soy is one of the source of the Fitoestrogen because the structure isoflavon of soy is similar with the structure of estrogen molecule, so it can confound the balancial of hormone. Soy also as a source of protein that rich of amino acid arginin. The study was carried out to find the effects of soy bean milk on spermatozoa qualities. This experimental study was conducted to nine wistar rats weighing from 200 to 250 grams. These nine wistar rats were divide into 3 groups. Consists of control group (K) that did not gives the soy bean milk, the group 1 (P1) that gives the soy bean milk with dose 500mg/kg BB/day and the group 2 (P2) with dose 780mg/kg BB/day. Treatment carried out for 60 days. As a result, there is an improvement in consentration and morphology of spermatozoa which are statistically significant (p<0,05) in group 1 (P1) and group 2 (P2). Spermatozoa motility have a tendency to rise, but statistically meaningless (p>0,05). Conclusion: The higher dose of soy bean milk may rise the concentration, morphology and motility of spermatozoa wistar rats.Keywords: soy bean, soy bean milk, qualities of spermatozoa.Abstrak: Pengaruh kacang kedelai terhadap kualitas spermatozoa masih menimbulkan kontroversi. Kedelai merupakan salah satu sumber fitoestrogen karena struktur isoflavon kedelai mirip dengan struktur molekul estrogen sehingga dapat mengacaukan keseimbangan hormon. Kedelai juga sebagai sumber protein yang kaya akan asam amino arginin. Penelitian ini bertujuan untuk mengetahui efek dari susu kacang kedelai terhadap kualitas spermatozoa tikus wistar. Penelitian ini menggunakan 9 ekor wistar yang terbagi secara acak ke dalam 3 kelompok. Terdiri dari Kelompok kontrol yang tidak diberikan susu kacang kedelai, kelompok perlakuan 1 (P1) yang diberi susu kacang kacang kedelai dengan dosis 500mg/kgBB/hari dan kelompok perlakuan 2 (P2) dengan dosis 780mg/kgBB/hari. Pemberian perlakuan berlangsung selama 60 hari. Hasil penelitian memperlihatkan peningkatan konsentrasi dan morfologi spermatozoa yang signifikan secara statistik (p<0,05) antara kelompok perlakuan 1 (P1) dan kelompok perlakuan 2 (P2). Motilitas spermatozoa pada kelompok perlakuan 1 (P1) dan kelompok perlakuan 2 (P2) mempunyai kecenderungan meningkat, tetapi secara statistik tidak bermakna (p>0,05). Simpulan: Makin tinggi dosis susu kacang kedelai yang diberikan, dapat meningkatkan konsentrasi, motilitas, dan morfologi spermatozoa tikus wistar.Kata kunci: kacang kedelai, susu kacang kedelai, kualitas spermatozoa.

scholarly journals Vitamin D Supplementation Associated to Better Survival in Hospitalized Frail Elderly COVID-19 Patients: The GERIA-COVID Quasi-Experimental Study

Nutrients ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3377 ◽  
Author(s):  
Gaëlle Annweiler ◽  
Mathieu Corvaisier ◽  
Jennifer Gautier ◽  
Vincent Dubée ◽  
Erick Legrand ◽  
...  
Keyword(s):  
Experimental Study ◽  
Vitamin D ◽  
Frail Elderly ◽  
Vitamin D Supplementation ◽  
Ordinal Scale ◽  
Geriatric Unit ◽  
Quasi Experimental ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Background. The objective of this quasi-experimental study was to determine whether bolus vitamin D supplementation taken either regularly over the preceding year or after the diagnosis of COVID-19 was effective in improving survival among hospitalized frail elderly COVID-19 patients. Methods. Seventy-seven patients consecutively hospitalized for COVID-19 in a geriatric unit were included. Intervention groups were participants regularly supplemented with vitamin D over the preceding year (Group 1), and those supplemented with vitamin D after COVID-19 diagnosis (Group 2). The comparator group involved participants having received no vitamin D supplements (Group 3). Outcomes were 14-day mortality and highest (worst) score on the ordinal scale for clinical improvement (OSCI) measured during COVID-19 acute phase. Potential confounders were age, gender, functional abilities, undernutrition, cancer, hypertension, cardiomyopathy, glycated hemoglobin, number of acute health issues at admission, hospital use of antibiotics, corticosteroids, and pharmacological treatments of respiratory disorders. Results. The three groups (n = 77; mean ± SD, 88 ± 5 years; 49% women) were similar at baseline (except for woman proportion, p = 0.02), as were the treatments used for COVID-19. In Group 1 (n = 29), 93.1% of COVID-19 participants survived at day 14, compared to 81.2% survivors in Group 2 (n = 16) (p = 0.33) and 68.7% survivors in Group 3 (n = 32) (p = 0.02). While considering Group 3 as reference (hazard ratio (HR) = 1), the fully-adjusted HR for 14-day mortality was HR = 0.07 (p = 0.017) for Group 1 and HR = 0.37 (p = 0.28) for Group 2. Group 1 had longer survival time than Group 3 (log-rank p = 0.015), although there was no difference between Groups 2 and 3 (log-rank p = 0.32). Group 1, but not Group 2 (p = 0.40), was associated with lower risk of OSCI score ≥5 compared to Group 3 (odds ratio = 0.08, p = 0.03). Conclusions. Regular bolus vitamin D supplementation was associated with less severe COVID-19 and better survival in frail elderly.

New Technique for Application of Fibrin Sealant: Rubbing Method Devised to Prevent Cerebrospinal Fluid Leakage from Dura Mater Sites Repaired with Expanded Polytetrafluoroethylene Surgical Membranes

Neurosurgery ◽  
2001 ◽  
Vol 49 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Satoshi Nakajima ◽  
Tadaharu Fukuda ◽  
Masamichi Hasue ◽  
Yuichi Sengoku ◽  
Jo Haraoka ◽  
...  
Keyword(s):  
Experimental Study ◽  
Cerebrospinal Fluid ◽  
Clinical Study ◽  
Fibrin Sealant ◽  
Cerebrospinal Fluid Leakage ◽  
Fluid Leakage ◽  
Pressure Elevation ◽  
Spray Method ◽  
Group 2 ◽  
Group 1

Abstract OBJECTIVE The effectiveness of a strong, pressure-tight method for fibrin sealant application was experimentally and clinically evaluated, using expanded polytetrafluoroethylene (ePTFE) surgical membranes. METHODS Two application methods were examined in two groups. For Group 1, 1 ml each of Solutions A and B was simply sprayed over the surface (spray method). For Group 2, a small amount of Solution A was rubbed into the sutured site and then Solutions A and B were sprayed over the surface (rubbing method). In the experimental study, a moment pressure elevation test and a continuous stepwise pressure elevation test were performed for both groups. The clinical study was conducted using 350 patients undergoing craniotomies. The ePTFE surgical membrane was used for 180 patients but not the other 170 patients. RESULTS In the experimental study, the bursting pressures were 51.4 ± 13.0 cm H2O for Group 1 and 129 ± 35.0 cm H2O for Group 2 in the moment pressure elevation test and 27.0 ± 6.7 cm H2O and 100 ± 31.9 cm H2O, respectively, in the continuous stepwise pressure elevation test. The sealing effect was significantly greater for Group 2 than for Group 1 in both tests (P &lt; 0.001). In the clinical study using ePTFE surgical membranes, the rubbing method significantly reduced the incidence of postoperative cerebrospinal fluid leakage (1.4%), compared with the spray method (18.3%), when external decompression was not concomitantly used (P &lt; 0.01). CONCLUSION The sealing effect of fibrin sealant on cerebrospinal fluid leakage was significantly greater with the rubbing method than with the spray method. The strong sealing effect obtained with the rubbing method is thought to result from firm linkage between the ePTFE surgical membrane and the fibrin sealant and also from fibrin being formed even in needle holes and spaces in the sutured site.

scholarly journals The Effect of Two-Mile Jogging Training on Lung Function Values in TNI Soldier Kodam I/Bukit Barisan

2020 ◽  
Vol 1 (1) ◽  
pp. 61-67
Author(s):  
Rifda Hayati ◽  
Nuryunita Nainggolan ◽  
Amira Permatasari Tarigan ◽  
Putri Chairani Eyanoer
Keyword(s):  
Experimental Study ◽  
Lung Function ◽  
Physical Exercise ◽  
Physical Endurance ◽  
Improve Lung Function ◽  
Research Subjects ◽  
Practice Group ◽  
Group 2 ◽  
Army Personnel ◽  
Group 1

Background: Military soldiers are army personnel who are required to have good physical endurance and optimal fitness, include. So, it's needed physical exercise, include to be able to improve lung function. One parameter that can reflect physical fitness are lung function and m VO2max. This study aims to determine whether there is a relationship between physical exercise in the form of 2-mile jogging with increased lung function and VO2max of military soldiers Kodam 1 Bukit Barisan Methods: This research is an experimental study carried out in February-April 2019 against the TNI soldiers of the Bukit Barisan Military District 1. The research subjects were divided into two groups, group 1 who underwent regular 2-mile jogging exercises 3-5 times a week with a duration of 25 minutes for 2 months, and group 2 who underwent non-routine jogging exercises (less than 3 times a week). Lung function was assessed by spirometry. Results: As many as 68 military soldiers were involved in this study which were divided into two groups, 38 people in the group 1 who routinely trained and 30 people in group 2 that did not routinely practice. Group 1 VEP1 values were higher than group 2 (p=0.03). No significant differences in terms of KVP, MVV and VO2max in both groups. Lung function of smokers were lower than non-smokers, but not statistically significant. Conclusion: There were significant differences in VEP1 values between military soldiers who routinely and did not routinely undergo 2-mile jogging exercises.

scholarly journals MORPHOLOGY OF GRAFTED TENDON

2011 ◽  
Vol 18 (01) ◽  
pp. 112-119
Author(s):  
SHAHZADA M.H JAFRI ◽  
ANJUM ANWAR QADRI ◽  
KHUBAIB SHAHZAD ◽  
Mulazim Hussain Bukhari
Keyword(s):  
Experimental Study ◽  
Study Design ◽  
Morphological Study ◽  
Morphological Changes ◽  
Two Stage ◽  
Group 3 ◽  
Group 2 ◽  
Fibrous Reaction ◽  
Morphologic Changes ◽  
Group 1

This is the first research done to explore the morphologic changes in two stage tendon grafting as compared to one stage tendon grafting. AIMS: To compare morphology of grafted tendons with and with out first stage silicon rubber rod implantation. STUDY DESIGN: Comparative experimental study. PERIOD: 1994-2007. MATERIAL AND METHODS: 30 patients were included in this study. They was divided into 3 groups. Group 1 underwent 2 stage tendon grafting group 3 was used as control morphological study of tendons. RESULTS: Group 1 (1-stage) tendon grafting showed degeneration and fibrous reaction as morphological changes. Group 2. (2 Stage) appeared as normal tendons morphologically. CONCLUSION: This study concludes that instead of direct tendon grafting, two stage tendon grafting is recommended.

scholarly journals Comparison of hemorrhoidectomy combined with LIS and open hemorrhoidectomy.

2020 ◽  
Vol 27 (11) ◽  
pp. 2305-2308
Author(s):  
Sajid Rashid ◽  
Naveed Malik
Keyword(s):  
Experimental Study ◽  
Pain Score ◽  
Study Design ◽  
P Value ◽  
Open Hemorrhoidectomy ◽  
Post Operative Pain ◽  
Group 2 ◽  
Study Setting ◽  
Surgical Unit ◽  
Group 1

Objectives: To measure the effectiveness of addition of LIS with open haemorrhoidectomy in reducing post-operative pain. Study Design: Experimental study. Setting: Surgical Unit of DHQ Teaching Hospital Rawalpindi. Period: Jan-2016 to Nov-2017. Material & Methods: This study was conducted on total number of 60 patients (n=60) with 4th degree haemorrhoids. Patients were divided into two equal groups of 30. In group 1 open haemorrhoidectomy with LIS was done and group 2 open haemorrhoidectomy without LIS was done. Results: Mean pain score of 2.53 with SD of 0.681 was found in group 1 (open haemorrhoidectomy with LIS) as compare to mean pain score of 7.07 with SD of 1.112 in group 2 (open haemorrhoidectomy without LIS) p value was 0.05. T-Test was applied and difference between the two groups was found statistically significant. Conclusion: Open haemorrhoidectomy with LIS reduces post-operative pain in patients.

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