Histological analysis of the repair of dural lesions with silicone mesh in rats subjected to experimental lesions

ABSTRACT Objective To evaluate inflammatory reaction, fibrosis and neovascularization in dural repairs in Wistar rats using four techniques: simple suture, bovine collagen membrane, silicon mesh and silicon mesh with suture. Methods Thirty Wistar rats were randomized in five groups: the first was the control group, submitted to dural tear only. The others underwent durotomy and simple suture, bovine collagen membrane, silicon mesh and silicon mesh with suture. Animals were euthanized and the spine was submitted to histological evaluation with a score system (ranging from zero to 3) for inflammation, neovascularization and fibrosis. Results Fibrosis was significantly different between simple suture and silicon mesh (p=0.005) and between simple suture and mesh with suture (p=0.015), showing that fibrosis is more intense when a foreign body is used in the repair. Bovine membrane was significantly different from mesh plus suture (p=0.011) regarding vascularization. Inflammation was significantly different between simple suture and bovine collagen membrane. Conclusion Silicon mesh, compared to other commercial products available, is a possible alternative for dural repair. More studies are necessary to confirm these findings.

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Flaxseed, olive and fish oil influence plasmatic lipids, lymphocyte migration and morphometry of the intestinal of Wistar rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502010000300010 ◽

2010 ◽

Vol 25 (3) ◽

pp. 275-280 ◽

Cited By ~ 12

Author(s):

Damiana Diniz Rosa ◽

Regiane Lopes de Sales ◽

Luis Fernando de Sousa Moraes ◽

Fabíola Cesário Lourenço ◽

Clóvis Andrade Neves ◽

...

Keyword(s):

Fish Oil ◽

Intestinal Mucosa ◽

Wistar Rats ◽

Density Lipoprotein ◽

Parameter Analysis ◽

Male Rats ◽

Histological Evaluation ◽

Control Group ◽

Soy Oil ◽

Lymphocyte Migration

PURPOSE: Evaluate the effect of flaxseed, olive and fish oil on the lipid profile, preservation of villosities and lymphocyte migration in the intestinal mucosa of Wistar rats. METHODS: Thirty Wistar male rats were divided into four groups, which received the AIN-93M diet, with changes only to their lipid source: flaxseed, olive, fish, and soy oil (control group). The serum was separated for the biochemical parameter analysis. A histological evaluation was performed in the ileal portion. RESULTS: The group which was fed fish oil presented lower values when compared to the other treatments for Total Cholesterol, High-density Lipoprotein Cholesterol and Triacylglycerol (p<0.05). The animals treated with fish and olive oils presented better intestinal villosities preservation. Less deposition of lymphocytes was observed in the flaxseed group (p<0.001). CONCLUSIONS: This study demonstrated that flaxseed, olive and fish oils present different responses than soy oil for the intestinal mucosa preservation and lymphocyte proliferation in Wistar rats.

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Morphological alterations in the heart and aorta of rats treated with glucocorticoids

Journal of Morphological Sciences ◽

10.4322/jms.065814 ◽

2015 ◽

Vol 32 (04) ◽

pp. 231-235

Author(s):

R. Dantas ◽

K. Souza ◽

D. Santos ◽

V. Feitosa ◽

E. Fioretto ◽

...

Keyword(s):

Wistar Rats ◽

Histological Analysis ◽

Saline Solution ◽

Morphological Changes ◽

Morphological Structure ◽

Control Group ◽

Morphological Alterations ◽

Heart Chamber ◽

Male Wistar Rats ◽

Synthetic Glucocorticoid

Abstract Introduction: The objective of this study was to analyze the morphological structure of the heart and aorta of rats treated with the synthetic glucocorticoid dexamethasone. Material and Methods: Male Wistar rats were divided into two groups: 08 control rats undergoing treatment with a 0.9% saline solution for 10 days and 08 rats treated for 10 days with dexamethasone (2mg/kg animal weight). Results: Histological analysis detected a mild cardiac hypertrophy and 15% reduction of collagen located in the aorta of animals treated with glucocorticoid when compared to the control group. Conclusion: We conclude that treatment with dexamethasone for a period of 10 consecutive days is able to promote morphological changes in the structure of the heart chamber and, impair morphological structure of aorta.

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Histological evaluation of the effects of corticotomy on induced orthodontic movement in rats

Revista de Odontologia da UNESP ◽

10.1590/1807-2577.10417 ◽

2017 ◽

Vol 46 (6) ◽

pp. 368-373 ◽

Cited By ~ 1

Author(s):

Patrícia Pigato SCHNEIDER ◽

Lais Pavan SILVA ◽

Fernando José Lopes de Campos CARVALHO ◽

Luís Filipe Siu LON ◽

Luiz Gonzaga GANDINI JÚNIOR

Keyword(s):

Histological Analysis ◽

Tooth Movement ◽

Initial Period ◽

Invasive Procedure ◽

Histological Evaluation ◽

Control Group ◽

Coil Spring ◽

Orthodontic Movement ◽

Spring Force ◽

Movement Group

Abstract: Introduction: Alveolar corticotomy is a surgical procedure used to increase the velocity of tooth movement. Objective Identify histological evidence of the effect of corticotomy on orthodontic movement in rats. Material and method Forty-five Wistar rats (Rattusnorvegicus Albinus) were equally divided into three groups: Control Group (CG) - no tooth movement or corticotomy; Movement Group (MG) - tooth orthodontic movement only; and Corticotomy and Movement Group (CMG) - tooth orthodontic movement surgically assisted by corticotomy. In the CMG, surgical procedures consisted in an incision in the palatal, reaching from the mesial to the distal regions of the maxillary right first molar. Tooth movement in the MG and CMG was applied with coil spring force of 40 gF from the maxillary right first molar to the maxillary right incisor. The rats were sacrificed at days 1, 3, and 7, and histological sections were performed to evaluate the counting of osteoblasts and osteoclasts throughout the areas of tension and pressure. Result Histological analysis showed that the CMG presented better cell response to bone neoformation compared with that of the other groups. Greater proliferation of osteoclasts was observed in areas of pressure on day 3, resulting in increased reabsorption, whereas greater proliferation of osteoblasts was observed in areas of tension on day 1, indicating increased bone formation. Conclusion Differences between the treated groups occurred only in the initial period of tooth movement. Therefore, the changes caused by corticotomy are not significant in orthodontic movement to justify this invasive procedure.

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Pool of bovine morphogenetic proteins and guided tissue regeneration in the treatment of intrabony periodontal defects: I- Clinical measurements

Journal of Applied Oral Science ◽

10.1590/s1678-77572004000100013 ◽

2004 ◽

Vol 12 (1) ◽

pp. 70-77 ◽

Cited By ~ 8

Author(s):

Maria do Carmo Machado Guimarães ◽

Euloir Passanezi ◽

Adriana Campos Passanezi Sant'Ana ◽

Sebastião Luiz Aguiar Greghi

Keyword(s):

Demineralized Bone Matrix ◽

Bone Matrix ◽

Test Group ◽

Control Group ◽

Collagen Membrane ◽

Bovine Bone ◽

Intrabony Defects ◽

Bovine Collagen ◽

Barrier Membrane ◽

Clinical Measurements

The purpose of this study was to evaluate the effect of the pool of bovine BMPs on the treatment of intrabony defects. The sample comprised 15 patients aged 26 to 57 years old presenting with 10 pairs of lesions of 2 or 3 walls or 2-3 walls ³5mm, located in the same type teeth (premolar or molar) and same jaw. The test defects were treated with combination of a pool of bovine bone morphogenetic and resorbable hydroxyapatite carrier (BMPs- HA), bovine demineralized bone matrix (MB) and coverage by a bovine collagen barrier membrane. The control defects were treated with MB-HA and covered by a bovine collagen membrane. The clinical measurements at six months after therapy in the test group revealed a reduction in the mean probing pocket depth (PPD) of 1.63 ± 1.41mm (B) and 1.93 ± 0.96mm (L) and a mean change in the clinical attachment level (CAL) of 1.60 ± 1.16mm (B) and 1.46 ± 0.97mm (L). The control group showed a mean reduction of PPD of 1.93 ± 1.34mm (B) and 2.0 ± 1.51mm (L) and a mean change of CAL of 1.03 ± 1.24mm (B) and 1.30 ± 1.14 mm (L). The analysis of variance (ANOVA) demonstrated that the changes in the clinical parameters were statistically significant (p<0.05). There were no significant differences between the test and controls subjects (Student's test, p<0.005). These findings suggest that the use of a pool of bovine BMPs do not provide added effects to GTR in the treatment of intrabony defects.

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Quercetin reduces tendon adhesion in rat through suppression of oxidative stress

BMC Musculoskeletal Disorders ◽

10.1186/s12891-020-03618-2 ◽

2020 ◽

Vol 21 (1) ◽

Author(s):

Yuan Liang ◽

Keteng Xu ◽

Pei Zhang ◽

Jiale Zhang ◽

Pengtao Chen ◽

...

Keyword(s):

Oxidative Stress ◽

Histological Analysis ◽

Histological Evaluation ◽

Control Group ◽

Biological Functions ◽

Tendon Tissue ◽

Safe Strategy ◽

Prevention And Treatment ◽

And Control ◽

Clinical Problems

Abstract Background Tendon adhesion is one of the most common clinical problems, which poses a considerable challenge to orthopedics doctors. Quercetin (QUE) as a popular drug at present, it has various biological functions, including anti-inflammatory, anti-ischemic, anti-peroxidation, and antioxidant. The purpose of this study was to investigate the effect of quercetin on tendon adhesion and whether quercetin can inhibit oxidative stress. Method Thirty-six rats were randomly divided into three groups, including control group, low QUE (50 mg/kg/day) group, and high QUE (100 mg/kg/day) group. After 1 week, the levels of SOD, MDA and GPx were measured. The degree of tendon adhesion was assessed by macroscopic evaluation and histological evaluation. After 4 weeks. Besides, the pharmacological toxicity of quercetin to main organs were evaluated by histological analysis. Results The extent of superoxide dismutase (SOD) and glutathione peroxidase (GPx) of tendon tissue in high QUE group was significantly higher than those of low QUE group and control group. And the extent of malondialdehyde (MDA) of tendon tissue in high QUE group was significantly lower than that of low QUE group and control group. By macroscopic evaluation and histological analysis, the extent of tendon adhesion in high QUE group was lower than low QUE group and control group. However, there were no significant changes of the major organs through histological analysis. Conclusions Quercetin may be a good and safe strategy in preventing tendon adhesion. But further clinical research is needed before its recommendation in the prevention and treatment of tendon adhesion.

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A Nutraceutical approach to enhance Reproductive longevity and Ovarian health using Curcuma longa in wistar Rats

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00939 ◽

2021 ◽

pp. 5385-5390

Author(s):

Gowda K M Damodara ◽

S Moodithaya Shailaja ◽

R Kedilaya Vishakh ◽

Nayanatara A K ◽

Suchetha Kumari N

Keyword(s):

Body Weight ◽

Wistar Rats ◽

Curcuma Longa ◽

Histological Evaluation ◽

Control Group ◽

Adult Rats ◽

Primordial Follicles ◽

Aging Properties ◽

Sham Control Group

Aging is associated with various physiological, pathological and psychosocial alterations. This study evaluates the benefits of Curcumin by assessing reproductive aging indices and ovarian health in Wistar rats. Laboratory bred adult rats selected for the experiment. After 12 months of follow-up, the animals were grouped into Normal control rats, Sham control group, Curcumin-1(100 mg/kg body weight), Curcumin-2(200 mg/kg body weight) and Curcumin-3 (400 mg/kg body weight). For the duration of six months Curcumin dosage was administered. The experimental parameters included estrous cycle and histological evaluation of ovarian follicles. Data were analyzed using one-way ANOVA. The percentage of primordial follicles was significantly more (p<0.001) in all the groups when compared with other types of follicles. Prolonged increase (p=0.0001) in the Diestrus phase in animals treated with different dosages of Curcumin. The current study concludes that Curcumin, an active component of Curcuma longa contributes to the anti-aging properties.

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Effects of dimethylsulfoxide and pentoxifylline in the vitality of cutaneous flaps in rats

Revista do Colégio Brasileiro de Cirurgiões ◽

10.1590/0100-69912017005006 ◽

2017 ◽

Vol 44 (5) ◽

pp. 457-464 ◽

Cited By ~ 1

Author(s):

Stephanie Luzia da Costa Pedretti ◽

Cícero de Lima Rena ◽

Maria Christina Marques Nogueira Castãnon ◽

Ana Paula do Nascimento Duque ◽

Fernando Henrique Pereira ◽

...

Keyword(s):

Single Dose ◽

Tissue Repair ◽

Wistar Rats ◽

Inflammatory Infiltrate ◽

Histological Analysis ◽

Distal Segment ◽

Repair Process ◽

Control Group ◽

Skin Flaps ◽

Cutaneous Flap

ABSTRACT Objectives: to verify the influence of dimethylsulfoxide and pentoxifylline on the vitality of cutaneous flaps in rats and the tissue repair process. Methods: were studied 30 Wistar rats, submitting them to a 2cm wide by 8cm long dorsal cutaneous flap, of caudal base. We distributed the animals in three groups: Control Group (n=10) with application gauze moistened with 0.9% Saline in the flap bed for 30 seconds; Dimethylsulfoxide group (n=10), with administration of 1ml of 5% dimethylsulfoxide divided into five injections of 0.2ml in the transition of the flap segments; Pentoxifylline group (n=10), with administration of pentoxifylline 20mg/kg, diluted to 1ml and divided into five injections of 0.2ml in the transition of the flap segments. Drugs were administered intraoperatively, in a single dose and subcutaneously. We observed the skin flaps for changes in color and texture. On the 10th postoperative day, we checked the dimensions of viable and necrotic tissues, followed by excision of the specimen for histological analysis. Results: the measurements of length of the viable and necrotic tissues between groups showed no differences. Histological analysis showed that the Dimethylsulfoxide group presented neovascularization, inflammatory infiltrate with leukocytes and more structured conjunctival stroma. The Pentoxifylline group showed neovascularization and inflammatory infiltrate, with moderate to intense granulation. The control group evolved with a higher rate of necrosis in the distal segment. Conclusion: dimethylsulfoxide and pentoxifylline influenced the vitality of the flap and the tissue repair process. However, they did not prevent necrosis macroscopically.

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Histological analysis of low-intensity laser therapy effects in peripheral nerve regeneration in Wistar rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502011000100004 ◽

2011 ◽

Vol 26 (1) ◽

pp. 12-18 ◽

Cited By ~ 35

Author(s):

Cibele Nazaré da Silva Câmara ◽

Marcus Vinicius Henriques Brito ◽

Edvaldo Lima Silveira ◽

Diego Sá Guimarães da Silva ◽

Vivian Roberta Ferreira Simões ◽

...

Keyword(s):

Sciatic Nerve ◽

Nerve Regeneration ◽

Laser Therapy ◽

Wistar Rats ◽

Histological Analysis ◽

Control Group ◽

Low Intensity ◽

Low Intensity Laser Therapy ◽

Sciatic Nerve Regeneration ◽

Intensity Laser

Purpose: Analyze the influence of low-intensity laser therapy in the sciatic nerve regeneration of rats submitted to controlled crush through histological analysis. Methods: Were used 20 Wistar rats, to analyze the influence of low-intensity laser therapy in the sciatic nerve regeneration, where the injury of the type axonotmesis was induced by a haemostatic clamp Crile (2nd level of the rack). The animals were randomly distributed in 2 groups. Control group (CG n = 10) and Laser group (LG n = 10). These were subdivided in 2 subgroups each, according to the euthanasia period: (CG14 _ n = 5 and CG21 _ n = 5) and (LG14 _ n = 5 and LG21 _ n = 5). At the end of treatment, the samples were removed and prepared for histological analysis, where were analyzed and quantified the following findings: Schwann cells, myelinic axons with large diameter and neurons. Results: In the groups submitted to low-intensity laser therapy, were observed an increase in the number of all analyzed aspects with significance level. Conclusion: The irradiation with low intensity laser (904nm) influenced positively the regeneration of the sciatic nerve in Wistar rats after being injured by crush (axonotmesis), becoming the nerve recovery more rapid and efficient.

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Animal models of ovariectomy in rats demonstrate characteristics similar to early stage osteoarthritis

10.21203/rs.3.rs-528848/v1 ◽

2021 ◽

Author(s):

Natália Aparecida Casonato ◽

Camila Marques de Araújo ◽

Mariane Santos Trevisan ◽

Cristina Arrais Lima ◽

Fernando Augusto Vasilceac

Keyword(s):

Animal Models ◽

Knee Joint ◽

Wistar Rats ◽

Early Stage ◽

Experimental Period ◽

Histological Evaluation ◽

Control Group ◽

Intermediate Region ◽

Histological Changes ◽

Deep Region

Abstract Purpose The aim of our study is to analyze the model of ovariectomy (OVX) in rats reproduced histological changes of osteoarthritis (OA). Methods For the development of the research, 12 Wistar rats were used, divided into 2 equal groups: Control Group - C (n = 6) and Osteoarthritis Group - OA (n = 6). After the 6-month experimental period, all rats were sacrificed and, subsequently, the entire knee joint complex was removed without disarticulation. For the histological evaluation of the tissue, the recommendations of the International Society for Research in OA (OARSI) were used. For data processing, each evaluation was statistically treated in both groups, comparing data from group C with the group OA. Results: Through the histological evaluation of OARSI, the evolution of OA in various tissues of the joint was evaluated. Although the OA group showed noticeable differences from group C, they were not as significant. Thus, only statistically significant favors were presented in the loss of the cartilaginous matrix (OA and C, p = 0.51), considering that the changes in the loss of ECM occurred only at the depth of 0% (superficial region), but at the depth of 50% (intermediate region) and 100% depth (deep region) did not exist. Conclusion Our study demonstrated that the OVX model is a good model to discuss OA, showing histological changes similar to those found in OA, the model demonstrated to have a progressive and slow characteristic since after the OARSI evaluations, prominent evidence was found in the initial manifestations of OA.

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Histological Analysis of Early Osteolysis in Total Ankle Arthroplasty

Foot & Ankle Orthopaedics ◽

10.1177/2473011417s000075 ◽

2017 ◽

Vol 2 (3) ◽

pp. 2473011417S0000 ◽

Cited By ~ 1

Author(s):

Oliver Schipper ◽

Steven Haddad

Keyword(s):

Foreign Body ◽

Giant Cell ◽

Histological Analysis ◽

Polyethylene Wear ◽

Giant Cells ◽

Foreign Body Giant Cell ◽

Control Group ◽

Wear Particles ◽

Cell Reaction ◽

Ankle Arthroplasty

Category: Ankle Arthritis Introduction/Purpose: The purpose of this study was to perform a histological comparative analysis of tibiotalar joint samples taken from areas of osteolysis adjacent to total ankle arthroplasties vs control synovial specimens to determine the reaction to and presence of polyethylene (PE) particles. Methods: A total of 57 pathology samples were identified in the osteolysis group, while 11 were identified in the control group. For each sample, hematoxylin and eosin, Oil Red O (ORO), and macrophage marker CD163-stained slides were created. Polarized light and ORO stain were used to identify PE particles. The presence of metal particles and giant cell reaction to PE particles were also scored. Results: Macrophages, PE particles, metallosis, and foreign body giant cell reaction scores were significantly higher in the osteolysis group compared with the control group. In the osteolysis group, ORO staining was positive in 93% (53/57), birefringent material was present in 96.5% (55/57), and macrophage infiltrates were present in 96.5% (55/57). Foreign body giant cell reaction with giant cells surrounding PE particles was present in 49.1% (28/57) of osteolytic specimens. The presence of foreign body giant cell reaction was associated with significantly higher macrophage, ORO, and polarizable material scores. The average time to surgery for osteolysis from the index ankle replacement was 6.0 (range, 0-15) years for the 57 patients in the osteolysis group. Conclusion: This study is the largest ankle arthroplasty histological analysis to show that areas of osteolysis consist of abundant polyethylene wear particles, present both intracellularly and extracellularly. Furthermore, these areas were associated with a CD163+ macrophage infiltrate and frequently a foreign body reaction with giant cells engulfing PE particles. It is likely that implant wear particles play a significant role in osteolysis based on the histopathology.

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