A Histological Evaluation of Novel Cyanoacrylate-Based β-TCP Composite in Rat Calvarial Defects

2006 ◽  
Vol 309-311 ◽  
pp. 1133-1136 ◽  
Author(s):  
Sang Bae Lee ◽  
Kyeong Jun Park ◽  
Doug Youn Lee ◽  
Jeong Jong Park ◽  
Jae Sun Hwang ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Weight Ratio ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Control Group ◽  
Filling Material ◽  
Fibrous Connective Tissue ◽  
Implantation Time ◽  
Filling Materials ◽  
Calvarial Defects

In this study, the osteoconductive properties of novel cyanoacrylate-based filling materials for bone defect were evaluated. A new filling material was prepared by mixing Histoacryl® and acid-treated -tricalcium phosphate ( -TCP). Mixing weight ratio of acid-treated -TCP to Histoacryl® was 5:1. 12 male Spraque-Dawley rats were used in this study. The animals were divided into 4 groups. Critical-sized calvarial defects (8 mm) were created in 9 animals, and then the defects were treated with dense pellet specimen, porous cement-like specimen, and untreated defect for surgical control group. Augmentation treatments were carried out in 3 animals. Histological analysis revealed excellent ostgeoconductive properties of new filling materials. But, some of -TCP particle in the cement-like group were encapsulated by fibrous connective tissue. For the dense pellet group and augmentation treatment group, shape and stability were better maintained during the implantation time than cement like group. These results indicate that our novel -TCP/Histoacryl® composite have the potential to serve as filling materials for bone defects in the dental and plastic surgery.

scholarly journals C-Phycocyanin Alleviates Bladder Inflammation and Dysfunction in Cyclophosphamide-Induced Cystitis in a Mouse Model by Inhibiting COX-2 and EP4

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Xing-qi Bao ◽  
Yi-chen Huang ◽  
Fang Chen
Keyword(s):  
Smooth Muscles ◽  
Weight Ratio ◽  
Inflammatory Factors ◽  
Histological Evaluation ◽  
Control Group ◽  
Prostaglandin E ◽  
Bladder Inflammation ◽  
Group A ◽  
Cox 2 ◽  
Micturition Frequency

Objective. To explore the effect of C-phycocyanin (C-PC) on voiding behavior and histological changes in cyclophosphamide- (CYP-) induced cystitis in mice. Methods. Sixty female mice were included. The mice in the C-PC group received C-PC (25 mg/kg, twice, i.p.) and then CYP (200 mg/kg, i.p.) two hours later, while the mice in the CYP group only received the equivalent CYP. Saline was injected in the mice in the control group. A voided stain on paper (VSOP) test was conducted to analyze the micturition. The bladders were harvested for histological evaluation and measurements of inflammatory factors. Results. C-PC reduced the micturition frequency in the mice with CYP-induced cystitis. The bladder/body weight ratio and edema were remarkably higher in the CYP group compared to the C-PC group. C-PC suppressed the expressions of COX-2, PGE2, and EP4 (prostaglandin E receptor 4) according to the ELISA assay. Immunohistochemical staining also indicated that C-PC reduced the expressions of COX-2 in urothelium and EP4 in smooth muscles. Conclusions. C-PC relieved symptoms associated with CYP-induced cystitis in mice by inhibiting bladder inflammation through COX-2 and EP4 expression.

scholarly journals Histological evaluation of the effects of corticotomy on induced orthodontic movement in rats

2017 ◽  
Vol 46 (6) ◽  
pp. 368-373 ◽  
Author(s):  
Patrícia Pigato SCHNEIDER ◽  
Lais Pavan SILVA ◽  
Fernando José Lopes de Campos CARVALHO ◽  
Luís Filipe Siu LON ◽  
Luiz Gonzaga GANDINI JÚNIOR
Keyword(s):  
Histological Analysis ◽  
Tooth Movement ◽  
Initial Period ◽  
Invasive Procedure ◽  
Histological Evaluation ◽  
Control Group ◽  
Coil Spring ◽  
Orthodontic Movement ◽  
Spring Force ◽  
Movement Group

Abstract: Introduction: Alveolar corticotomy is a surgical procedure used to increase the velocity of tooth movement. Objective Identify histological evidence of the effect of corticotomy on orthodontic movement in rats. Material and method Forty-five Wistar rats (Rattusnorvegicus Albinus) were equally divided into three groups: Control Group (CG) - no tooth movement or corticotomy; Movement Group (MG) - tooth orthodontic movement only; and Corticotomy and Movement Group (CMG) - tooth orthodontic movement surgically assisted by corticotomy. In the CMG, surgical procedures consisted in an incision in the palatal, reaching from the mesial to the distal regions of the maxillary right first molar. Tooth movement in the MG and CMG was applied with coil spring force of 40 gF from the maxillary right first molar to the maxillary right incisor. The rats were sacrificed at days 1, 3, and 7, and histological sections were performed to evaluate the counting of osteoblasts and osteoclasts throughout the areas of tension and pressure. Result Histological analysis showed that the CMG presented better cell response to bone neoformation compared with that of the other groups. Greater proliferation of osteoclasts was observed in areas of pressure on day 3, resulting in increased reabsorption, whereas greater proliferation of osteoblasts was observed in areas of tension on day 1, indicating increased bone formation. Conclusion Differences between the treated groups occurred only in the initial period of tooth movement. Therefore, the changes caused by corticotomy are not significant in orthodontic movement to justify this invasive procedure.

scholarly journals Quercetin reduces tendon adhesion in rat through suppression of oxidative stress

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Yuan Liang ◽  
Keteng Xu ◽  
Pei Zhang ◽  
Jiale Zhang ◽  
Pengtao Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Histological Analysis ◽  
Histological Evaluation ◽  
Control Group ◽  
Biological Functions ◽  
Tendon Tissue ◽  
Safe Strategy ◽  
Prevention And Treatment ◽  
And Control ◽  
Clinical Problems

Abstract Background Tendon adhesion is one of the most common clinical problems, which poses a considerable challenge to orthopedics doctors. Quercetin (QUE) as a popular drug at present, it has various biological functions, including anti-inflammatory, anti-ischemic, anti-peroxidation, and antioxidant. The purpose of this study was to investigate the effect of quercetin on tendon adhesion and whether quercetin can inhibit oxidative stress. Method Thirty-six rats were randomly divided into three groups, including control group, low QUE (50 mg/kg/day) group, and high QUE (100 mg/kg/day) group. After 1 week, the levels of SOD, MDA and GPx were measured. The degree of tendon adhesion was assessed by macroscopic evaluation and histological evaluation. After 4 weeks. Besides, the pharmacological toxicity of quercetin to main organs were evaluated by histological analysis. Results The extent of superoxide dismutase (SOD) and glutathione peroxidase (GPx) of tendon tissue in high QUE group was significantly higher than those of low QUE group and control group. And the extent of malondialdehyde (MDA) of tendon tissue in high QUE group was significantly lower than that of low QUE group and control group. By macroscopic evaluation and histological analysis, the extent of tendon adhesion in high QUE group was lower than low QUE group and control group. However, there were no significant changes of the major organs through histological analysis. Conclusions Quercetin may be a good and safe strategy in preventing tendon adhesion. But further clinical research is needed before its recommendation in the prevention and treatment of tendon adhesion.

scholarly journals The Effect of Autologous Platelet-Rich Plasma on Bone Regeneration by Autologous Mesenchymal Stem Cells Loaded onto Allogeneic Cancellous Bone Granules

2017 ◽  
Vol 203 (6) ◽  
pp. 327-338 ◽  
Author(s):  
Min Wook Joo ◽  
Seung Jae Chung ◽  
Seung Han Shin ◽  
Yang-Guk Chung
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Cancellous Bone ◽  
Platelet Rich Plasma ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Control Group ◽  
Micro Ct ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet

To develop a clinically effective bone regeneration strategy, we compared bone regeneration using allogeneic cancellous bone granule scaffolds loaded with autologous bone marrow-derived mesenchymal stem cells (BM-MSC) with or without autologous platelet-rich plasma (PRP). Critical-sized segmental bone defects were made at the mid-shaft of both radiuses in 41 New Zealand White rabbits. Small-sized allogeneic cancellous bone granules (300-700 μm in diameter) loaded with BM-MSC were implanted on one side, and PRP was added. On the other side, autologous BM-MSC loaded onto allogeneic cancellous granules were grafted as a control. Bone regeneration was assessed by radiographic evaluation at 4, 8, and 16 weeks postimplantation and by micro-computed tomography (micro-CT) and histological evaluation of the retrieved specimens at 8 and 16 weeks. The experimental group did not show significantly higher bone quantity indices than the control group at any time point. Micro-CT analysis revealed that both groups had similar mean total volumes, surface areas, and other parameters at 8 and 16 weeks. Histological evaluation of 8- and 16-week specimens also showed a similar progression of new bone formation and maturation. In this experiment using a contralateral control group in the same individual, an initial single addition of PRP in allogeneic cancellous bone granules loaded with BM-MSC for critical-sized bone defects in the weight-bearing area did not induce a consequent difference in bone healing. Further research into the optimal preparation and application of PRP is necessary. Furthermore, studies involving a greater number of subjects and larger experimental animals could determine the clinical relevance of PRP treatment.

Bioactive Cyanoacrylate-Based Filling Material for Bone Defects in Dental Applications

2005 ◽  
Vol 284-286 ◽  
pp. 933-936 ◽  
Author(s):  
Kyeong Jun Park ◽  
Ji Ho Park ◽  
Sang Bae Lee ◽  
Doug Youn Lee ◽  
Kyoung Nam Kim ◽  
...  
Keyword(s):  
Bond Strength ◽  
Temperature Change ◽  
Tricalcium Phosphate ◽  
Shear Bond Strength ◽  
Bone Defects ◽  
Distilled Water ◽  
Filling Material ◽  
Filling Materials ◽  
Bioactive Properties ◽  
Dental Applications

We tried to prepare a new filling material for bone defects using β-Tricalcium phosphate (β-TCP) particles and Histoacryl®. The aim of this study was to evaluate physical and bioactive properties of cyanoacrylate-based filling materials for bone defects in the dental field. The shear bond strength values of the Histoacryl® and β-TCP/ Histoacryl® compounds stored in double-distilled water decreased with the increase of the amount of added β-TCP. The temperature change of the β-TCP/ Histoacryl® compounds during polymerization decreased compared to that of the Histoacryl®. The cytotoxicity of the filling materials decreased when the amount of added β-TCP was increased. In the evaluation of bioactivity, hydroxyapatite (HA) was precipitated on the surface and inner space of the porous filling material 4 weeks after immersion in SBF. This precipitation of HA on the surface of the filling material was also confirmed in the XRD result. These results indicate that our novel β-TCP/Histoacryl® compounds have the potential to serve as filling materials for bone defects in the dental field.

scholarly journals Verification of remaining filling materials in flattened/oval-shaped root canals after use of several endodontic retreatment protocols

RSBO ◽  
2021 ◽  
Vol 17 (2) ◽  
pp. 72-78
Author(s):  
Fábio Carmona Tirintan ◽  
Álvaro Henrique Borges ◽  
Jesus Djalma Pécora ◽  
Manoel dos Santos da Silva Neto ◽  
Matheus Gehrke Barbosa ◽  
...  
Keyword(s):  
Complete Removal ◽  
Lower Amount ◽  
Control Group ◽  
Filling Material ◽  
Root Canal Filling ◽  
Root Canals ◽  
Filling Materials ◽  
Level Of Significance ◽  
Relative Differences ◽  
Endodontic Retreatment

This study verified of remaining filling materials in flattened/oval-shaped root canals after use of several endodontic retreatment protocols. Material and methods: Seventy human mandibular incisors were selected. Sixty specimens received crown-apex preparation performed by ProTaper Next® system andwere filled with hybrid Tagger technique with gutta-percha and AH Plus®. Ten specimens were left unprepared (Control Group). The specimens were randomly distributed in six groups, according the different retreatment protocols: G1 – Clearsonic® + ProTaper Universal Retreatment® + Reciproc®; G2 – Reciproc®; G3 – Clearsonic® + ProTaper Universal Retreatment®; G4 – Clearsonic® + Reciproc®; G5 – Oscillatory EndoEze® + Manual files; G6 – ProTaper Universal Retreatment®. Presence of root canal filling material residual were evaluated using Scanning Electron Microscopy. The relative differences of remaining filling materials between the groups and root thirds were determined by Kolmogorov-Smirnov and Levene tests followed by Tukey post hoc test. The level of significance was set at α = 5%. Results: Regarding the presence residual, G1 (Clearsonic® + ProTaper Universal Retreatment® + Reciproc®), G2 (Reciproc®), G3(Clearsonic® + ProTaper Universal Retreatment®) and G6 (ProTaper Universal Retreatment®) protocols presented a lower amount (p<0.05). The highest values (p<0.05) of remaining filling materials were presented in the G5 (Oscillatory EndoEze® + Manual files) protocol. Intermediate values (p<0.05) were presented by G4 (Clearsonic® + Reciproc®) protocol. Conclusion: The endodontic retreatment protocols no showed effectiveness for complete removal of remaining sealing material from flattened/oval-shaped root canals. 

scholarly journals An experimental model for the study of craniofacial deformities

2010 ◽  
Vol 25 (3) ◽  
pp. 264-268 ◽  
Author(s):  
André de Mendonça Costa ◽  
Gerson Shigeru Kobayashi ◽  
Daniela Franco Bueno ◽  
Marília Trierveiler Martins ◽  
Marcus de Castro Ferreira ◽  
...  
Keyword(s):  
Experimental Model ◽  
Dura Mater ◽  
Wistar Rats ◽  
Histological Analysis ◽  
Bone Defects ◽  
Cranial Bone ◽  
Craniofacial Abnormalities ◽  
Tissue Samples ◽  
Surgical Model ◽  
Calvarial Defects

PURPOSE: To develop an experimental surgical model in rats for the study of craniofacial abnormalities. METHODS: Full thickness calvarial defects with 10x10-mm and 5x8-mm dimensions were created in 40 male NIS Wistar rats, body weight ranging from 320 to 420 g. The animals were equally divided into two groups. The periosteum was removed and dura mater was left intact. Animals were killed at 8 and 16 weeks postoperatively and cranial tissue samples were taken from the defects for histological analysis. RESULTS: Cranial defects remained open even after 16 weeks postoperatively. CONCLUSION: The experimental model with 5x8-mm defects in the parietal region with the removal of the periosteum and maintenance of the integrity of the dura mater are critical and might be used for the study of cranial bone defects in craniofacial abnormalities.

scholarly journals Effects of collagen/β-tricalcium phosphate bone graft to regenerate bone in critically sized rabbit calvarial defects

2019 ◽  
Vol 17 (1) ◽  
pp. 228080001882049 ◽  
Author(s):  
Hamid Tebyanian ◽  
Mohammad Hadi Norahan ◽  
Hossein Eyni ◽  
Mansoureh Movahedin ◽  
SM Javad Mortazavi ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Regeneration ◽  
Bone Repair ◽  
Bone Growth ◽  
Bone Defects ◽  
Histological Evaluation ◽  
Material Degradation ◽  
Alizarin Red ◽  
Calvarial Defects ◽  
Hematoxylin And Eosin

Bone defects remain a significant health issue and a major cause of morbidity in elderly patients. Composites based on collagen/calcium phosphate have been widely used for bone repair in clinical applications, owing to their comparability to bone extracellular matrix. This study aimed to evaluate the effects of a scaffold of collagen/calcium phosphate (COL/β-TCP) on bone formation to assess its potential use as a bone substitute to repair bone defects. Bilateral full-thickness critically sized calvarial defects (8 mm in diameter) were created in New Zealand white rabbits and treated with COL/β-TCP or COL scaffolds. One defect was also left unfilled as a control. Bone regeneration was assessed through histological evaluation using hematoxylin and eosin and Masson’s trichrome staining after 4 and 8 weeks. Alizarin Red staining was also utilized to observe the mineralization process. Our findings indicated that COL/β-TCP implantation could better enhance bone regeneration than COL and exhibited both new bone growth and scaffold material degradation.

scholarly journals Histological Assessment of an Experimental Model of Human Osteomyelitis in Rabbits

2018 ◽  
Vol 1 (1) ◽  
pp. 432-437
Author(s):  
Cristin Coman ◽  
Carmen Cristina Surdu-Bob ◽  
Florica Barbuceanu ◽  
Ene Vlase ◽  
Marius Badulescu
Keyword(s):  
Staphylococcus Aureus ◽  
Histological Analysis ◽  
Bone Defects ◽  
Experimental Models ◽  
Histological Evaluation ◽  
Separate Study ◽  
Histological Assessment ◽  
Rabbit Tibia ◽  
Histopathological Evaluation ◽  
Histological Data

Abstract The aim of this paper was to present a histological evaluation of the treatment performed on a humanmodel of osteomyelitis induced in rabbits. Osteomyelitis was induced in rabbits by a human strain of Staphylococcus aureus injected in bone defects created in the rabbit tibia. There have been created five groups of animals, groups receiving treatment in two different stages of the disease, acute and chronic. Copper and silver sub-millimetre-particles were introduced in the same place with the Staphylococcus solution. Evaluation of installation and evolution of the disease was done by clinical, hematological, microbiological, radiological and histological monitoring. A separate study of histological data is presented here. Histological examinations performed by HE was done on 5 μm sections of uncalcified bone. Every examination was classified according to a system of score. The results of histopathological evaluation confirm that histological analysis is a powerful tool in experimental models of this disease.

scholarly journals Histological analysis of the repair of dural lesions with silicone mesh in rats subjected to experimental lesions

2015 ◽  
Vol 13 (4) ◽  
pp. 567-573
Author(s):  
Fernando William Figueiredo da Rosa ◽  
Pedro Henrique Isoldi Pohl ◽  
Ana Maria Amaral Antônio Mader ◽  
Carla Peluso de Paiva ◽  
Aline Amaro dos Santos ◽  
...  
Keyword(s):  
Foreign Body ◽  
Wistar Rats ◽  
Histological Analysis ◽  
Histological Evaluation ◽  
Control Group ◽  
Collagen Membrane ◽  
Dural Tear ◽  
Dural Repair ◽  
Score System ◽  
Bovine Collagen

ABSTRACT Objective To evaluate inflammatory reaction, fibrosis and neovascularization in dural repairs in Wistar rats using four techniques: simple suture, bovine collagen membrane, silicon mesh and silicon mesh with suture. Methods Thirty Wistar rats were randomized in five groups: the first was the control group, submitted to dural tear only. The others underwent durotomy and simple suture, bovine collagen membrane, silicon mesh and silicon mesh with suture. Animals were euthanized and the spine was submitted to histological evaluation with a score system (ranging from zero to 3) for inflammation, neovascularization and fibrosis. Results Fibrosis was significantly different between simple suture and silicon mesh (p=0.005) and between simple suture and mesh with suture (p=0.015), showing that fibrosis is more intense when a foreign body is used in the repair. Bovine membrane was significantly different from mesh plus suture (p=0.011) regarding vascularization. Inflammation was significantly different between simple suture and bovine collagen membrane. Conclusion Silicon mesh, compared to other commercial products available, is a possible alternative for dural repair. More studies are necessary to confirm these findings.

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