Flaxseed, olive and fish oil influence plasmatic lipids, lymphocyte migration and morphometry of the intestinal of Wistar rats

PURPOSE: Evaluate the effect of flaxseed, olive and fish oil on the lipid profile, preservation of villosities and lymphocyte migration in the intestinal mucosa of Wistar rats. METHODS: Thirty Wistar male rats were divided into four groups, which received the AIN-93M diet, with changes only to their lipid source: flaxseed, olive, fish, and soy oil (control group). The serum was separated for the biochemical parameter analysis. A histological evaluation was performed in the ileal portion. RESULTS: The group which was fed fish oil presented lower values when compared to the other treatments for Total Cholesterol, High-density Lipoprotein Cholesterol and Triacylglycerol (p<0.05). The animals treated with fish and olive oils presented better intestinal villosities preservation. Less deposition of lymphocytes was observed in the flaxseed group (p<0.001). CONCLUSIONS: This study demonstrated that flaxseed, olive and fish oils present different responses than soy oil for the intestinal mucosa preservation and lymphocyte proliferation in Wistar rats.

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The Efficacy of Seluang Fish Oil (Rasbora agrotynea) Related Body Weight, Lipid Profile, Adiponectin and Leptin in Wistar Rats-Induced High Fat Diet

BioScientia Medicina ◽

10.32539/bsm.v2i2.41 ◽

2018 ◽

Vol 2 (2) ◽

pp. 25-30

Author(s):

Fatmawati Karim ◽

Rachmat Hidayat ◽

Erizka Rivani ◽

Husnul Khotimah ◽

Ester G Pansserga

Keyword(s):

Body Weight ◽

Fish Oil ◽

Lipid Profile ◽

High Fat Diet ◽

Wistar Rats ◽

Adiponectin Level ◽

Male Rats ◽

Control Group ◽

High Fat ◽

Reduce Body Weight

Background Rasbora agrotynea (local name : Seluang ) is a fish native to Sumatera, Malaya and Borneo. This fish has a potential as a local wisdom for supplementation of omega 3 and omega 6. In Sumatera, Seluang fish was used as traditional food. Aim of Study Aim of this study to explore the efficacy of seluang fish oil in body weight, lipid profile, adiponectin and leptin level in Wistar Rats-Induced High Fat Diet Methods This study was an experimental study , pre-post test with control group design. The sample in this study was 30 male rats, 8 weeks old, weight 150-200 gram. Rats were given high fat diet and seluang fish oil at dose of 0,1 , 0,2 and 0,4 mL/200 gr BW/day for 2 weeks. Seluang fish oil was extracted by distilation methods. The results of this study were assayed by SPSS 18. Results Seluang fish oil 0,4 mL/200 gr BW was more potent to reduce body weight gain, triglyseride level, leptin and increase adiponectin level than seluang fish oil 0,1 mL/200 gr BW, 0,2 mL/200 gr BW, negative control and positive control. Conclusion Seluang fish oil had a potention to reduce body weight, triglyceride, leptin and increase adiponectin level. Keywords: Seluang fish Oil â€“ body weight- triglyserida â€“ leptin - adiponectin

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Effect of Turmeric and Ginger on Lipid Profile in Male Rats Exposed to Oxidative Stress

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.10.1.23 ◽

2019 ◽

Vol 10 (01) ◽

Author(s):

Eman A. Al-Rekabi ◽

Dheyaa K. Alomer ◽

Rana Talib Al-Muswie ◽

Khalid G. Al-Fartosi

Keyword(s):

Oxidative Stress ◽

Hydrogen Peroxide ◽

Drinking Water ◽

Lipid Profile ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Male Rats ◽

Control Group ◽

Very Low Density Lipoprotein ◽

Low Density

The present study aimed to investigate the effect of turmeric and ginger on lipid profile of male rats exposed to oxidative stress induced by hydrogen peroxide H2O2 at a concentration of 1% given with consumed drinking water to male rats. Methods: 200 mg/kg from turmeric and ginger were used, and the animals were treatment for 30 days. Results: the results showed a significant increase in cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), whereas it explained a significant decrease in high density lipoprotein (HDL) of male rats exposed to oxidative stress when compared with control group. the results showed a significant decrease in cholesterol, triglycerides, (LDL), (VLDL), whereas it explained a significant increase in (HDL) of rats treated with turmeric and ginger at dose 200 mg/kg when compared with male rats exposed to oxidative stress.

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Toxicological evaluation of hydroethanol leaf extract of Pupalia lappacea (Linn.) Juss. (Amaranthaceae) in rodents

Drug Metabolism and Personalized Therapy ◽

10.1515/dmdi-2021-0115 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Murtala Akanji Abdullahi ◽

Elijah Oladapo Oyinloye ◽

Akinyinka Alabi ◽

Aderonke Adeyinka Aderinola ◽

Luqman Opeyemi Ogunjimi ◽

...

Keyword(s):

Leaf Extract ◽

Density Lipoprotein ◽

Serum Testosterone ◽

Female Rats ◽

Male Rats ◽

Laboratory Animals ◽

Serum Testosterone Level ◽

Control Group ◽

Toxicological Evaluation ◽

Liver And Kidney

Abstract Objectives Several studies have established the ethnobotanical benefits of Pupalia lappacea (PL) in laboratory animals without extensive toxicological evaluation of its safety profiles. Thus, an extensive toxicological investigation of sub-chronic oral administration of the hydroethanol leaf extract of P. lappacea in rodents was carried out in this study. Methods Different groups of rats were treated orally with the extract (10, 50 and 250 mg/kg) daily for 90 consecutive days. The control group received distilled water (10 mL/kg). After 90 days, some rats were left for additional 30 days without treatment for reversibility study. Blood and organs samples were collected for different evaluations at the end of study periods. Results The extract decreased the bodyweights, feeding and water intakes in female rats. PL increased the weights of the liver and kidney in male rats. PL increased the red blood cell (RBC), packed cell volume (PCV), hemoglobin (Hb), triglycerides (TRIG), cholesterol and high density lipoprotein (HDL) contents in rats. PL (250 mg/kg) significantly reduced the sperm motility and serum testosterone level. Cyto-architectural distortions of the testes, liver and spleen were visible. Conclusions The findings showed that P. lappacea is relatively safe at lower doses but cautions should be taken at higher dose.

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RESVERATROL INCLUSION COMPLEX WITH β-CYCLODEXTRIN (RCD): CHARACTERIZATION AND EVALUATION OF TOXICITY IN WISTAR RATS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i1.15399 ◽

2016 ◽

Vol 9 (1) ◽

pp. 27 ◽

Cited By ~ 2

Author(s):

V. S. K. Nishihira ◽

N. J. Mezzomo ◽

M. D. Baldissera ◽

R. A. Vaucher ◽

C. G. Pinto ◽

...

Keyword(s):

Inclusion Complex ◽

Oral Administration ◽

Biochemical Parameters ◽

Wistar Rats ◽

Metabolic Diseases ◽

Hematological Parameters ◽

Density Lipoprotein ◽

Blood Parameters ◽

Control Group ◽

Scanning Calorimetry

Objective: The aim of this study was to characterise the resveratrol inclusion complex with β-cyclodextrin (RCD) and evaluate their toxicity in wistar rats.Methods: The RCD were prepared in ultra-turrax. For characterization of the RCD were used: Fourier transform infra-red Spectroscopy, Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimetry (DSC) and X-ray powder diffraction. The RCD and others 4 treatments were performed by the chronic oral administration in 35 rats during 60 ds. After the treatments they were euthanized and the serum blood were collected to analyzed some hemogram and biochemical parameters including aspartyl aminotransferase (AST); alanine aminotransferase (AST); phosphatase alkaline (ALP); total bilirubin (TB); direct bilirubin (DB); total protein (TP); total cholesterol (TC), triacylglycerol (TAG), very low-density lipoprotein (VLDL), high-density lipoprotein (HDL), calcium, iron and phosphate using fully automated biochemistry analyzer.Results: The characterization results indicated a successful formation of the RCD. All hematological parameters analysed were within the normal values in all the groups. Furthermore, the hemogram and biochemical parameters were significantly (P>0.05) similar to the control group.Conclusion: The daily oral administration during 60 d of RCD are not harmful on blood parameters of Wistar rats. Thus, RCD can be used safely for treatment of some metabolic diseases.

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Upregulation of Kiss-1 and Gpr54 Genes Expression in Pituitary of Male Rats Following the Central Administration of Neuropeptide Y

International journal of basic science in medicine ◽

10.34172/ijbsm.2019.03 ◽

2019 ◽

Vol 4 (4) ◽

pp. 137-142

Author(s):

Vahid Azizi ◽

Shahrbanoo Oryan ◽

Homayuon Khazali ◽

Abdolkarim Hosseini

Keyword(s):

Gene Expression ◽

Pituitary Gland ◽

Neuropeptide Y ◽

Wistar Rats ◽

Third Ventricle ◽

Male Rats ◽

Control Group ◽

Central Administration ◽

Reproductive Axis ◽

Male Wistar Rats

Introduction: The neuropeptide Y (NPY) in the neural circuits of the hypothalamus has a stimulating effect on reproductive activities in mammals. Kisspeptin (KiSS1) is a quintessential neurotransmitter in the reproductive axis which directly stimulates gonadotropin-releasing hormone neurons in the hypothalamus. The distribution of KiSS1 expressing cells in the pituitary was described previously. Despite earlier reports showing the KiSS1 receptor, G-protein coupled receptor 54 (GPR54) expression in the pituitary, the potential physiological roles of kisspeptin at this gland have remained obscure. Accordingly, this study investigated the role of NPY on the relative expression of Kiss1 and Gpr54 genes in the pituitary gland in male Wistar rats. Methods: In general, 20 male Wistar rats weighing 200-250 g in 4 groups (5 in each group) received saline, NPY (2.3 nM), BIBP3226 (NPY receptor antagonist, 7.8 nM), and NPY+ BIBP3226. Then, they received the simultaneous injection of these molecules through the third ventricle of the brain. Finally, the relative mean expressions of Kiss1 and Gpr54 genes in the anterior pituitary were quantitatively analyzed by the real-time polymerase chain reaction. Results: The central injection of NPY increased the relative mean expressions of Kiss1 and Gpr54 genes in the pituitary gland compared to the control group although the injection of BIBP3226 eradicated these effects. However, the gene expression of Gpr54 in the rats receiving NPY coupled with BIBP3226 in hypophysis in comparison to the group receiving only NPY demonstrated a significant reduction (P<0.05). Conclusion: Overall, the central injection of NPY stimulated the gene expression of Kiss1 and Gpr54 in the pituitary gland.

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PENGARUH PEMBERIAN QUERCETIN TERHADAP KADAR LEPTIN SERUM TIKUS WISTAR YANG DIBERI DIET TINGGI LEMAK

Saintika Medika ◽

10.22219/sm.v6i2.1019 ◽

2012 ◽

Vol 6 (2) ◽

Author(s):

Sudiarto . ◽

Sri Hidayati Suprihatin

Keyword(s):

Metabolic Syndrome ◽

Wistar Rats ◽

Block Design ◽

Density Lipoprotein ◽

Control Group ◽

Diet Induced Obesity ◽

Post Test ◽

Ultimate Cause ◽

Chronical Disease ◽

High Level

Obesity, one of the degenerative diseases, is a risk factor for chronical disease and an ultimate cause of metabolic syndrome which ismarked by the raising level of leptin on blood (hyperleptinemia). Metabolic syndrome is marked by the size of the waist is more than 40 inchfor man or 35 inch for woman, hypertension, hyperglycemia, high level of triglyceride, and the level of HDL (high-density lipoprotein) is low.This research is aimed to know the giving of quercetin (which has high anti oxide activities) on the declining level of leptin to the giving of DIO(Diet Induced Obesity). An experimental study using Post Test Control Group was done to the experimental wistar rats. Samples werechosen by using randomized complete block design. Rats were treated for 8 weeks. The dose of given quercetin were 2 mg/kgBB/day, 10mg/kgBB/day, and 50 mg/kgBB/day during the last 8 weeks of research. The variable measured in this research is the level of leptin onblood. Based on the experiment, quercetin is proven that gives influence to the level of leptin on obesity wistar rats’ serum compare to the givenlevel of leptin on controlled rats. There is different average on the level of leptin on wistar rats’ serum which being obesity on each grouptreatment. The giving of quercetin have significant correlation (p<0.05) with the level of leptin on wistar rats’ serum which being obesity (r = -0.704, p = 0.001). The conclusion from this research is the giving of quercetin to obesity rats for 8 weeks can decline the level of leptin onblood.Keywords: quercetin, leptin, high fat diet

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Correlation between Serum and Tissue Levels of Adipokines in Obesity in Adult Male Rats with and without Antioxidant

QJM ◽

10.1093/qjmed/hcaa065.011 ◽

2020 ◽

Vol 113 (Supplement_1) ◽

Author(s):

M M Elshawwa

Keyword(s):

Insulin Resistance ◽

Adult Male ◽

Fasting Glucose ◽

Density Lipoprotein ◽

Serum Levels ◽

Lipoprotein Cholesterol ◽

Male Rats ◽

Control Group ◽

Tissue Levels ◽

Group I

Abstract Background Obesity is associated with insulin resistance, type2 diabetes, dyslipidemia and cardiovascular diseases. Apelin and chemerin are identified as adipokines and adipose tissue markers. Several adipose-derived peptides are known to influence food intake, including apelin, whose expression is regulated by insulin and chemerin. Oxidative stress thought to be involved in the development of complications associated with obesity. Objective To study the nature of correlation between serum and liver levels of apelin, chemerin and oxidative parameters in obese rats with and without antioxidant. Aiming to clarify the pathophysiology of obesity. Material and Methods Thirty adult male albino rats, divided into three equal groups. Group I (control), group II (obese) and group III (obese and Lepidium sativum (LS) as an antioxidants). At the end of the experiment, blood samples were collected for estimation of the serum levels of chemerin, apelin, fasting glucose, insulin, insulin resistance (IR), lipid profile, reduced glutathione (GSH) and malondialdehyde (MDA). In addition to tissue homogenous extracts of liver were taken for the levels of MDA, CAT, chemerin and apelin. Results After eight weeks, high fat diet group showed a significant increase in serum levels of apelin, chemerin, fasting glucose, insulin, IR, total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C) & MDA and a significant decrease in high-density lipoprotein cholesterol (HDL-C) & GSH. HFD also caused a significant increase in tissue levels of MDA, CAT & chemerin and a significant decrease in apelin, compared to control group. While addition of LS to HFD caused a significant decrease in serum levels of apelin, chemerin, fasting glucose, insulin, IR, TC, TG, LDL-C & MDA and a significant increase in HDL-C & GSH. LS also caused a significant decrease in tissue levels of MDA, chemerin & insignificant decrease in CAT and a significant increase in apelin, compared to HFD group. Conclusion This study showed a significant positive correlation between liver & serum chemerin and between liver and serum MDA. On the other hand, it showed a significant negative correlation between liver and serum apelin and liver CAT and serum GSH

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Effect of Prunus Dulcis & Álpha-Tocopherol in Ethanol Induced Dyslipidemia In Wistar Rats

Biomedical & Pharmacology Journal ◽

10.13005/bpj/2037 ◽

2020 ◽

Vol 13 (4) ◽

pp. 1619-1624

Author(s):

Samuel Sundar Doss ◽

J. Vijayakumar ◽

E. Sukumar ◽

K. Rekha

Keyword(s):

Wistar Rats ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Prunus Dulcis ◽

Alpha Tocopherol ◽

Control Group ◽

P Value ◽

Low Density ◽

Ethanol Group ◽

Beneficial Effects

The study is aimed at assessing the effect of Prunus dulcis and alpha-tocopherol treatment against ethanol induced dyslipidemia in Wistar rats. 30 albino Wistar rats were selected based on the selection criteria and equally distributed into 5 groups – Control, ethanol, Prunus dulcis, alpha-tocopherol and combination of alpha-tocopherol + Prunus dulcis treated for 40 days. After the treatment for 40 days, all the animals were euthanized and a retro-orbital puncture was made to collect the blood samples for biochemical investigations. Obtained results were statistically analysed using ANOVA. Compared to ethanol group alpha tocopherol, Prunus dulcis and alpha tocopherol + Prunus dulcis treatment significantly decreased total cholesterol and triglycerides levels with p value <0.001. High density lipoprotein (66.31%) levels in the ethanol group were decreased compared to the control group and were significantly increased in other groups. Low density lipoprotein and Very low density lipoprotein levels were higher in the ethanol group compared with the control group and were significantly reduced in other groups with p value <0.001. Results suggest that ethanol has an ill effect on the lipid profile. Treatment with Prunus dulcius and alpha-tocopherol both solely or in combination has produced beneficial effects against dyslipidemia.

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Effects of Nori- and Wakame-enriched meats with or without supplementary cholesterol on arylesterase activity, lipaemia and lipoproteinaemia in growing Wistar rats

British Journal Of Nutrition ◽

10.1017/s000711451100198x ◽

2011 ◽

Vol 106 (10) ◽

pp. 1476-1486 ◽

Cited By ~ 24

Author(s):

Raúl Olivero-David ◽

Adriana Schultz-Moreira ◽

Miguel Vázquez-Velasco ◽

Laura González-Torres ◽

Sara Bastida ◽

...

Keyword(s):

Wistar Rats ◽

Plasma Cholesterol ◽

Dietary Cholesterol ◽

Lipoprotein Metabolism ◽

Density Lipoprotein ◽

Control Group ◽

Vldl Cholesterol ◽

Freeze Dried ◽

Cholesterol Supplementation ◽

Two Parameters

Some seaweeds exert antioxidant and hypocholesterolaemic properties. The effects of diets including restructured meats (RM) containing Wakame (W) or Nori (N) algae on arylesterase (AE) activity and lipoprotein concentration and composition were tested. In the present study, six groups of ten male growing Wistar rats each were fed a mix of 85 % AIN-93M diet and 15 % freeze-dried RM for 35 d. The control group (C) consumed control RM, the W and N groups consumed RM with 5 % W and 5 % N, respectively. The cholesterol-enriched C (CC), W (CW) and N (CN) groups consumed their corresponding basal diets with supplementary cholesterol (2·43 %) and cholic acid (0·49 %). Cholesterol in the diet induced lower (P < 0·001) growth ratios. Both W and N diets significantly increased AE activity. VLDL-cholesterol values were lower in N rats than in W rats. AE activity increased (P < 0·001) in CC and CW rats but not in CN rats compared with their corresponding counterparts. AE was lower (P < 0·05) in the CN group than in the CC and CW groups. The CN diet partially blocked (P < 0·001) the hypercholesterolaemic induction observed in CC and CW diets and reduced TAG levels (at least P < 0·05) with respect to those of CC rats. Although dietary cholesterol supplementation increased total cholesterol, VLDL-cholesterol and (intermediate-density lipoprotein+LDL)-cholesterol (all P < 0·001) in all rats, the CN diet moderately improved the lipoprotein profile of hypercholesterolaemic rats. Changes in AE activity and plasma cholesterol in CN rats but not in CW rats suggest a possible relationship between the two parameters. It is concluded that inclusion of RM enriched with N may be used in hypercholesterolaemic diets to improve lipoprotein metabolism.

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Doxorubicin for prevention of epineurial fibrosis in a rat sciatic nerve model: outcome based on gross postsurgical, histopathological, and ultrastructural findings

Journal of Neurosurgery Spine ◽

10.3171/2009.9.spine09407 ◽

2010 ◽

Vol 12 (3) ◽

pp. 327-333 ◽

Cited By ~ 11

Author(s):

Baki S. Albayrak ◽

Ozgur Ismailoglu ◽

Konuralp Ilbay ◽

Umut Yaka ◽

Gamze Tanriover ◽

...

Keyword(s):

Human Subjects ◽

Electron Microscopic Examination ◽

Male Rats ◽

Surrounding Tissue ◽

Histological Evaluation ◽

Control Group ◽

Electron Microscopic ◽

Biceps Femoris Muscle ◽

Ultrastructural Studies ◽

Sciatic Nerves

Object Epineural fibrosis may complicate peripheral nerve surgeries and currently is considered as one of the main factors responsible for failed surgeries. The authors investigated the postoperative antiscarring effects of topically applied doxorubicin (DXR) on rat sciatic nerves. Methods The sciatic nerves were dissected from the surrounding tissue and exposed bilaterally in 20 Wistar albino adult male rats. Abrasion trauma was produced on the exposed surface of the biceps femoris muscle in the vicinity of the sciatic nerves and their main branches in all animals. In the DXR Group, cottonoid pads soaked with DXR (0.5 mg/ml) were placed around the nerves for 5 minutes, whereas cotton pads soaked with saline (0.9% NaCl) were applied to nerves of animals in the Control Group for the same duration. Twelve weeks after the procedure, all of the rats were killed and the sciatic nerves were examined. Epineural adhesions were evaluated histopathologically and ultrastructurally. Additionally, quantitative histological parameters, the scar tissue formation index and the scar density, were calculated in histological evaluation. Results Gross postsurgical evaluation as well as histopathological and electron microscopic examination of involved nerve segments showed significantly less epineurial adhesions in the DXR Group than in the Control Group. Quantitative analysis of the epineurium revealed a statistically significant reduction in the density and amount of epineural scarring in specimens from the DXR Group than in those from the Control Group. Conlusions The results of gross postsurgical anatomical evaluation and histopathological and ultrastructural studies suggested that topical application of DXR effectively reduced epineural scar formation on rat sciatic nerves. These promising findings merit further experimental and clinical studies to determine the efficacy and safe applicability of DXR in human subjects.

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