scholarly journals Electrical conductivity as an indicator of pea seed aging of stored at different temperatures

2007 ◽  
Vol 64 (2) ◽  
pp. 119-124 ◽  
Author(s):  
Maristela Panobianco ◽  
Roberval Daiton Vieira ◽  
Dilermando Perecin
Keyword(s):  
Electrical Conductivity ◽  
Low Temperatures ◽  
Storage Temperature ◽  
Membrane Integrity ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Storage Period ◽  
Cellular Membrane ◽  
Membrane Repair ◽  
Seed Deterioration

Research on soybean seed has revealed that conductivity test results may be influenced by storage temperature and that an apparent direct relationship between deterioration at low temperatures and loss of cellular membrane integrity is not evident. The objective of this study was to verify the influence of low storage temperatures on electrical conductivity results in other species, such as seeds of two pea cultivars studied temperatures were: 10, 20, 25, 20/10 and 25/10ºC. The physiological potential of the seeds was evaluated every three months during an 18 month storage period using germination, accelerated aging and electrical conductivity tests. The electrical conductivity test was not a good indicator for the degree of seed deterioration stored at low temperatures. Seed deterioration at 10ºC did not seem to be directly related to the loss of membrane integrity, possibly due to membrane repair or reorganization during storage at this temperature.

scholarly journals Is it possible to estimate longevity through the analyses used to measure the initial physiological potential in soybean seeds?

2021 ◽  
Vol 43 ◽  
Author(s):  
Thiago Barbosa Batista ◽  
Samara Moreira Perissato ◽  
Carlos Henrique Queiroz Rego ◽  
Gustavo Roberto Fonseca de Oliveira ◽  
Fernando Augusto Henning ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Linear Regression ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Storage Period ◽  
High Potential ◽  
Seed Longevity ◽  
Soybean Seeds ◽  
Radicle Protrusion ◽  
Over Time

Abstract: The analysis of longevity can support decisions about the length of seed lot storage until commercialization, since this characteristic implies the maintenance of viability over time. Seed longevity is analyzed by the p50 test, which expresses the time to lose 50% of the initial viability. Seeds with high vigor and germination have greater physiological potential and, thus, a greater capacity to maintain quality throughout the storage period. However, there has been little research on the correlations between the analysis of p50 (longevity) and the tests used to measure physiological potential (germination and vigor) of lots, which can be used as a tool to make inferences about longevity using the most traditional tests. Thus, the objective of this study was to investigate which tests used to measure the potential of lots can estimate p50. To this end, germination and vigor were evaluated using traditional tests while longevity was assessed in eight soybean seed lots. Correlations and linear regression were tested for the traditional variables versus p50. It was found that the use of accelerated aging, electrical conductivity, and time to 50% radicle protrusion has high potential to estimate longevity as measured by p50.

scholarly journals Temperature during soybean seed storage and the amount of electrolytes of soaked seeds solution

2008 ◽  
Vol 65 (5) ◽  
pp. 496-501 ◽  
Author(s):  
Roberval Daiton Vieira ◽  
Dennis Merlin TeKrony ◽  
Dennis Bruce Egli ◽  
William Patrick Bruenning ◽  
Maristela Panobianco
Keyword(s):  
Electrical Conductivity ◽  
Storage Temperature ◽  
Soybean Seed ◽  
Storage Period ◽  
Seed Storage ◽  
Seed Vigor ◽  
Soybean Seeds ◽  
Glycine Max L ◽  
Pea Seeds ◽  
And Storage

The electrical conductivity test measures the electrolytes that leach out of seeds when they are immersed in water and this leakage is an indication of seed vigor. The level of standardization reached by the procedures of this test is such that the test is recommended for pea seeds and suggested for other large seeded legumes, including soybean [Glycine max (L.) Merrill]. This study was conducted to contribute to the standardization of this test for soybean seeds by verifying whether the seed storage temperature influences the composition of the leachate from soaked seeds solution. Two soybean seed lots of distinct physiological potential were stored in moisture-proof containers either at constant temperatures of 10ºC and 20ºC or at the temperature of 20ºC during the first seven months of storage followed by a change to 10ºC for the rest of the storage time (nine months). The chemical composition of the soaked water was evaluated every three months from January to October 1998. The highest amount of leakage was observed for potassium, followed by calcium and magnesium, iron and sodium regardless of temperature and storage period. The amount of electrolytes in the soaked water increased as the period of time and the temperature of storage increased. On the other hand the amount of leakage decrease along the time for those seeds stored at 10ºC or transferred from the temperature of 20 to that of 10ºC. The temperature at which soybean seeds remain during storage may affect the amount of electrolytes in the soaked water and consequently the results of the electrical conductivity test.

scholarly journals Electrical conductivity testing of corn seeds as influenced by temperature and period of storage

2006 ◽  
Vol 41 (10) ◽  
pp. 1551-1559 ◽  
Author(s):  
Simone Aparecida Fessel ◽  
Roberval Daiton Vieira ◽  
Mara Cristina Pessoa da Cruz ◽  
Rinaldo Cesar de Paula ◽  
Maristela Panobianco
Keyword(s):  
Electrical Conductivity ◽  
Storage Temperature ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Corn Seed ◽  
Seed Deterioration ◽  
Deterioration Process ◽  
Physiological Quality ◽  
Effects Of Temperature ◽  
Plot Design

The objective of this work was to evaluate the effects of temperature (10, 20, 30, 20/10 and 30/10ºC) and period of storage on electrical conductivity (EC) in four seed lots of corn (Zea mays L.), as well as the mineral composition of the soaking solution. EC test determines indirectly the integrity of seed membrane systems, and is used for the assessment of seed vigor, because this test detects the seed deterioration process since its early phase. The research comprised determinations of water content, germination, accelerated aging (AA), cold (CT) and EC vigor tests, and determinations of Ca2+, Mg2+ and K+ release to the solution, after seed soaking of four corn seed lots. The evaluations were performed each four months during a period of 16 months. For statistical analysis, a completely randomized split plot design was used with eight replications. Except for seed lots stored at 10ºC, all vigor evaluations revealed a decline in vigor, but AA and CT showed more sensitiveness to declines of seed physiological quality than EC. Potassium was the main leached ion regardless of the storage temperature.

scholarly journals Eletrical conductivity and deterioration of soybean seeds exposed to different storage conditions

2007 ◽  
Vol 29 (2) ◽  
pp. 97-105 ◽  
Author(s):  
Maristela Panobianco ◽  
Roberval Daiton Vieira
Keyword(s):  
Electrical Conductivity ◽  
Fatty Acids ◽  
Storage Temperature ◽  
Membrane Integrity ◽  
Accelerated Aging ◽  
Storage Conditions ◽  
Soybean Seeds ◽  
Cell Membrane Integrity ◽  
Deterioration Process ◽  
Storage Temperatures

Research with soybean seeds has revealed that the results of the electrical conductivity test may be influenced by storage temperature, particularly low temperature, such as 10ºC, suggesting that seed deterioration at low storage temperatures does not seem to be directly related to the loss of the cell membrane integrity. This study was conducted with seeds of two soybean cultivars with the objective of: a) studying the effect of different storage temperatures (10ºC; 20ºC; 25ºC; 20/10ºC and 25/10ºC) on the results of the electrical conductivity test; b) observing the behavior of fatty acids and carbohydrates during storage and studying its relation with the electrical conductivity results. Every three months, from a total of 18 months of storage, the physiological quality of seeds was evaluated using the germination, accelerated aging and electrical conductivity tests. Based on the obtained results, it can be concluded that the electrical conductivity test was not shown to be a good indicative of the deterioration process of seeds stored at low temperatures, and no direct relationship between changes in the fatty acids and carbohydrates and the behavior of the mentioned test for seeds stored at 10ºC was found.

scholarly journals Electrical conductivity of the seed soaking solution and soybean seedling emergence

2004 ◽  
Vol 61 (2) ◽  
pp. 164-168 ◽  
Author(s):  
Roberval Daiton Vieira ◽  
Angelo Scappa Neto ◽  
Sonia Regina Mudrovitsch de Bittencourt ◽  
Maristela Panobianco
Keyword(s):  
Electrical Conductivity ◽  
Water Content ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Soybean Seedling ◽  
Seed Soaking ◽  
Laboratory Conditions ◽  
Field And Laboratory Conditions

Vigor of soybean [Glycine max (L.) Merrill] seeds can be evaluated by measuring the electrical conductivity (EC) of the seed soaking solution, which has shown a satisfactory relationship with field seedling emergence, but has not had aproper definition of range yet. This work studies the relationship between EC and soybean seedling emergence both in the field and laboratory conditions, using twenty two seed lots. Seed water content, standard germination and vigor (EC, accelerated aging and cold tests) were evaluated under laboratory conditions using -0.03; -0.20; -0.40 and -0.60 MPa matric potentials, and field seedling emergence was also observed. There was direct relationship between EC and field seedling emergence (FE). Under laboratory conditions, a decreasing relationship was found between EC and FE as water content in the substrate decreased. Relationships between these two parameters were also found when -0.03; -0.20 and -0.40 MPa matric potentials were used. EC tests can be used successfully to evaluate soybean seed vigor and identify lots with higher or lower field emergence potential.

scholarly journals Influence of lignin content in soybean seed coat on the incidence of the storage fungus Aspergillus flavus

2012 ◽  
Vol 34 (4) ◽  
pp. 541-548 ◽  
Author(s):  
Ísis Barreto Dantas ◽  
João Almir de Oliveira ◽  
Heloisa Oliveira dos Santos ◽  
Édila Vilela Resende Von Pinho ◽  
Sttela Dellyzete Veiga Franco da Rosa
Keyword(s):  
Electrical Conductivity ◽  
Aspergillus Flavus ◽  
Seed Coat ◽  
Seed Quality ◽  
Lignin Content ◽  
Soybean Seed ◽  
Storage Period ◽  
Seed Storage ◽  
Seed Health ◽  
Physiological Quality

Seed quality may be affected by several factors, including permeability, color, and lignin content in the seed coat. This study aimed at evaluating influence of lignin content in the tegument of seed samples of six different soybean cultivars, in which half of each sample was inoculated with the fungus Aspergillus flavus, on the physical and physiological quality, and on the seed health, during 180 days storage period, under cold chamber with controlled conditions of temperature and RH. For that, at each interval of 60 days, samples were removed, and the physiological quality of these seeds was assessed by means of moisture and lignin contents; and by tests of seed health, germination, and electrical conductivity. The moisture content of seeds remained constant during all storage period. In the seed health test, it was found that inoculation was efficient, once the minimum incidence of the fungus in the inoculated seeds was 85%. In the germination test, there was a trend of reduction on percentage germination with the increase in storage period. However, there was an increase on electrical conductivity of seeds assessed. It was concluded that there is no interference of the lignin content in the seed coat on the resistance to infection by the fungus Aspergillus flavus, even after seed storage for a period of 180 days.

scholarly journals Biochemical Profile of the Soybean Seed Embryonic Axis and Its Changes during Accelerated Aging

Biology ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 186
Author(s):  
Luciano Antônio Ebone ◽  
Andréia Caverzan ◽  
Diógenes Cecchin Silveira ◽  
Luciano de Oliveira Siqueira ◽  
Nadia Canali Lângaro ◽  
...  
Keyword(s):  
Ascorbate Peroxidase ◽  
Cell Structure ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Embryonic Axis ◽  
Nutrient Analysis ◽  
Maillard Reactions ◽  
Seed Deterioration ◽  
Randomized Experimental Design ◽  
Peroxidase Enzymes

Seed deterioration is an important topic in plant science, as the majority of cultivated species use seeds as their means of propagation; however, due to its complexity, the process of seed deterioration has not yet been completely elucidated. Three soybean cultivars (BMX Raio, BMX Zeus, and DM 53i54) exposed to four distinct periods of accelerated aging (0, 3, 6 and 9 days) in a fully randomized experimental design. Initially, vigor and germination tests were performed. The activity of superoxide dismutase, catalase, ascorbate peroxidase enzymes, hydrogen peroxide, malonaldehyde, DNA oxidation, macromolecules and mineral content, and Maillard reactions were quantified in the embryonic axis. Results showed that DNA did not suffer degradation or oxidation. In terms of consumption of reserves, only sugars were consumed, while levels of protein, starch, and triglycerides were maintained. The Maillard reaction did show potential as an indicator of buffer capacity of protein to ROS. Additionally, levels of catalase and ascorbate peroxidase decreased during the aging process. Moreover, nutrient analysis showed that a high magnesium level in the cultivar bestowed greater resilience to deterioration, which can indicate a potential function of magnesium in the cell structure via reflex in seed aging through seed respiration.

scholarly journals Evaluation of the physiological quality of anadenenthera peregrina (L.) Speg. seeds during storage

2009 ◽  
Vol 33 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Daniel Santos Pinho ◽  
Eduardo Euclydes de Lima e Borges ◽  
Viviana Borges Corte ◽  
Luiz Carlos Bhering Nasser
Keyword(s):  
Electrical Conductivity ◽  
Storage Temperature ◽  
Storage Period ◽  
Seed Priming ◽  
Seed Moisture ◽  
Physiological Quality ◽  
Viable Seeds ◽  
Cold Chamber ◽  
Constant Percentage

The aim of this experiment was to evaluate the effect of storage temperature on the viability and vigour of A. peregrina seeds. Seeds of A. peregrina harvested in Viçosa-MG, in September 2005 were used. The seeds were stored in the Laboratory of Forest Seeds Analysis (LASF) of the Universidade Federal de Viçosa (UFV), in a cold chamber, under approximately 5ºC over150 days. The seeds were stored under 20ºC for 150 days more. Seed moisture was determined after oven-drying at 105 ± 3ºC for 24 hours. The seeds were analyzed monthly using germination, tetrazolium and electrical conductivity tests and Osmopriming during 300 days, with four repetitions of 25 seeds. A. peregrina seeds kept a constant percentage of germination and viable seeds under the tetrazolium test for five months of storage, under either 5ºC and 20ºC. The electrical conductivity test showed significant differences in relation to the storage period, proving to be more sensitive than the standard germination test. Seed priming in PEG -0,4 MPa solution was not efficient in recovering the viability and vigor of the stored seeds at 5ºC and 20ºC.

scholarly journals Electrical conductivity test in the evaluation of the physiological potential of treated and stored soybean seeds

2021 ◽  
Vol 42 (6) ◽  
pp. 3135-3148
Author(s):  
Ana Paula Silva Couto ◽  
◽  
Cristian Rafael Brzezinski ◽  
Julia Abati ◽  
Ronan Carlos Colombo ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Seed Treatment ◽  
Seed Quality ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Control Treatment ◽  
Soybean Seeds ◽  
Seed Treatments ◽  
Chemical Treatments ◽  
Soybean Cultivars

Soybean seed treatment contributes to the maintenance of seed quality, but the effect of commercial formulations and chemical products on the effectiveness of the electrical conductivity test based on electrolyte leaching has been frequently questioned. This study aimed to verify the interference of the chemical seed treatment of two soybean cultivars on the effectiveness of the electrical conductivity test in evaluating the vigor of freshly treated and stored seeds. The experimental design was completely randomized, consisting of seven seed treatments and two evaluation periods (0 and 60 days after storage), with four replications. The used seed treatments consisted of 1) fipronil + pyraclostrobin + thiophanate-methyl, 2) imidacloprid + thiodicarb + carbendazim + thiram, 3) abamectin + thiamethoxan + fludioxonil + mefenoxam + thiabendazole, 4) carbendazim + thiram, 5) fludioxonil + mefenoxam + thiabendazole, 6) carboxin + thiram, and 7) control (no treatment). The cultivars were BRS 360 RR and BRS 284, which were analyzed separately. Germination, accelerated aging, emergence, and electrical conductivity tests were carried out. No differences were detected between the control and chemical treatments performed on seeds of the two freshly treated soybean cultivars regarding germination, accelerated aging, and emergence tests. The germination test stood out after storage with the cultivar BRS 360 RR, showing the maintenance of germination potential for seeds treated with carbendazim + thiram and the control treatment. Therefore, the chemical treatment of soybean seeds interferes with the result of the electrical conductivity test. The electrical conductivity test is effective in segregating seed lots in terms of vigor level. The electrical conductivity test correlates with the other vigor tests used to identify the reduction in the physiological seed quality with storage.

scholarly journals Harvest delay, storage and physiological quality of soybean seeds

2019 ◽  
Vol 41 (4) ◽  
pp. 506-513
Author(s):  
Rafael Vergara ◽  
Raimunda Nonata Oliveira da Silva ◽  
Arieli Paula Nadal ◽  
Gizele Ingrid Gadotti ◽  
Tiago Zanatta Aumonde ◽  
...  
Keyword(s):  
Seed Quality ◽  
Local Development ◽  
Block Design ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Storage Period ◽  
Seed Vigor ◽  
Soybean Seeds ◽  
Randomized Block Design ◽  
Harvest Delay

Abstract: Soybean is one of the leading commodities in Brazilian agribusiness. Its cultivation is widespread in different seed-producing regions of the country, where it generates income and local development. In this context, the soybean seed is a fundamental input, as its quality strongly influences the success of the crop. However, the period after the achievement of physiological maturity is critical for the maintenance of seed quality. For that reason, this study aimed at evaluating the effect of harvest delay on both the initial and final qualities of soybean seeds. The research consisted of four harvest times, in which the initial quality was assessed through tests of first germination count, germination, accelerated aging and tetrazolium. After 120 days of storage, the germination and accelerated aging tests were once again conducted. The experiment complied with a completely randomized block design with eight replications. The rainfall was monitored during the pre-harvest phase. All variables experienced negative impacts due to the delay in harvesting. Also, the seeds suffered more damage as the delay progressed, and the variables germination and seed vigor decreased after the storage period.

Sign in / Sign up

Export Citation Format

Share Document