Degradability study of neutral detergent fiber of coast cross (Cynodon dactylon x Cynodon nlemfunensis)

Abstract Early and late season kudzu (Pueraria montana var. lobata) leaves were collected to analyze dry matter (DM), crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF) in situ rumen disappearance to assess kudzu quality. Four studies were conducted during different growing seasons [2 repeated early season (ES); 2 repeated late season (LS) to determine age variability effects. Kudzu collected from 7 middle Tennessee counties were incubated in the rumen for designated lengths of time (0.25, 1, 12, 24, 36, 48, and 72 h) to determine total rumen degradation (%) and rate of disappearance (% / h). Data were analyzed as a randomized complete block design (RCBD) with repeated measures. Regression analysis was used to determine degradation rate. Overall season effects (across incubation times) for dry matter disappearance (DMD) were significantly different (P < 0.0001). Significant season by incubation time interactions were exhibited for total DMD at 12 (P < 0.0001), 24 (P = 0.0004), 36 (P = 0.0055), 48 (P = 0.0209), and 72 h (P = 0.0384) incubation, with an average rumen degradability of ES kudzu being 9.75% higher than LS between 12 and 72 h. Slope regression between 1 and 24 h determined a rumen degradation rate of 2.41% / h across both seasons, with no significant seasonal difference (P = 0.3396). These results are indicative that ES kudzu is more highly rumen degradable than LS, as evidenced by the significant season by incubation time interactions between 12 and 72 h of incubation. Although there are significant seasonal effects on kudzu DMD during certain incubation times, DMD overall for ES and LS is still indicative of high rumen degradability with an average of 81.45% at 72 h across both seasons. Season effects were minimal for NDF, ADF, and CP rumen disappearance.

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148 The influence of kudzu (Pueraria montana var. lobata) age on in situ rumen degradation

Journal of Animal Science ◽

10.1093/jas/skz053.085 ◽

2019 ◽

Vol 97 (Supplement_1) ◽

pp. 38-38

Author(s):

Joseph Gulizia ◽

Kevin Downs ◽

Song Cui

Keyword(s):

Incubation Time ◽

Repeated Measures ◽

Dry Matter ◽

Degradation Rate ◽

Block Design ◽

Neutral Detergent Fiber ◽

Late Season ◽

Rumen Degradation ◽

Pueraria Montana

Abstract Early and late season kudzu (Pueraria montana var. lobata) leaves were collected to analyze dry matter (DM), crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF) in situ rumen disappearance to assess kudzu quality. Four studies were conducted during different growing seasons [2 repeated early season (ES); 2 repeated late season (LS) to determine age variability effects. Kudzu collected from 7 middle Tennessee counties were incubated in the rumen for designated lengths of time (0.25, 1, 12, 24, 36, 48, and 72 h) to determine total rumen degradation (%) and rate of disappearance (% / h). Data were analyzed as a randomized complete block design (RCBD) with repeated measures. Regression analysis was used to determine degradation rate. Overall season effects (across incubation times) for dry matter disappearance (DMD) were significantly different (P < 0.0001). Significant season by incubation time interactions were exhibited for total DMD at 12 (P < 0.0001), 24 (P = 0.0004), 36 (P = 0.0055), 48 (P = 0.0209), and 72 h (P = 0.0384) incubation, with an average rumen degradability of ES kudzu being 9.75% higher than LS between 12 and 72 h. Slope regression between 1 and 24 h determined a rumen degradation rate of 2.41% / h across both seasons, with no significant seasonal difference (P = 0.3396). These results are indicative that ES kudzu is more highly rumen degradable than LS, as evidenced by the significant season by incubation time interactions between 12 and 72 h of incubation. Although there are significant seasonal effects on kudzu DMD during certain incubation times, DMD overall for ES and LS is still indicative of high rumen degradability with an average of 81.45% at 72 h across both seasons. Season effects were minimal for NDF, ADF, and CP rumen disappearance.

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In situ caecal degradation of roughages in horses

Revista Brasileira de Zootecnia ◽

10.1590/s1516-35982010000200018 ◽

2010 ◽

Vol 39 (2) ◽

pp. 349-355 ◽

Cited By ~ 2

Author(s):

Vinícius Pimentel Silva ◽

Fernando Queiroz de Almeida ◽

Eliane da Silva Morgado ◽

Liziana Maria Rodrigues ◽

Tiago Marques dos Santos ◽

...

Keyword(s):

Cynodon Dactylon ◽

Pigeon Pea ◽

Neutral Detergent Fiber ◽

Desmodium Ovalifolium ◽

Randomized Design ◽

Degradation Rates ◽

Digestion Technique ◽

In Situ Degradation ◽

The Times

The present study was carried out to evaluate the in situ degradation of dry matter (DM), neutral detergent fiber (NDF) and crude protein (CP) in roughages by the in situ caecal digestion technique in horses. The roughages evaluated were: Lucerne hay (Medicago sativa), peanut (Arachis pintoi cv. Amarillo), desmodio(Desmodium ovalifolium), stylo (Stylosanthes guianensis cv. Mineirão), pigeon pea (Cajanus cajan), lime-yellow pea (Macrotyloma axillare) and coastcross hay (Cynodon dactylon cv. coastcross). The assay was conducted in a complete randomized design with seven roughages and three replications. One mare with a cannula fitted in the caecum was used, fed diet consisting of coastcross hay (80%) and concentrate (20%) at 2.0% BW, four times a day. Nylon 6.5 × 20 cm bags were used with 45 μ/pore, containing 5.2 g DM/bag, inserting 3 or 4 bags in the caecum at the times of 2, 4, 6, 8, 12, 24 and 48 hours incubation. The caecum in situ degradability parameters of nutrients were obtained by Ørskov model. The DM degradability parameters of all the roughages were significant. There was no fit to the model for pigeon pea for CP and NDF and desmodio. Peanut, stylo and lime-yellow pea presented larger potentially degradable DM with values of 53, 46.5 and 40%, respectively, and higher values for the soluble fraction of 20, 21, 28.6%, with high degradability rates of 10.36, 20.26 and 14.8% h-1. Higher NDF degradation rates were observed in these foodstuffs with values of 9.1 and 11.3, 11.2% h-1, high potentially degradable fraction with values of 55, 51.8 and 47.2%, and greater CP degradation at 48 hours with values of 87, 95, and 94.8%. Peanut, stylo and lime-yellow pea presented potential for use in horses diets.

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PSI-35 Comparison of in situ techniques to evaluate the recovery of indigestible components and the accuracy of digestibility estimates

Journal of Animal Science ◽

10.1093/jas/skz258.517 ◽

2019 ◽

Vol 97 (Supplement_3) ◽

pp. 254-254

Author(s):

Jordan Adams ◽

Aaron B Norris ◽

Luis O Tedeschi

Keyword(s):

Neutral Detergent Fiber ◽

Parameter Estimates ◽

Surface Area Ratio ◽

In Situ Techniques ◽

Integral Role ◽

Fecal Recovery ◽

Internal Marker ◽

Internal Markers

Abstract Our objective was to evaluate the effects of bag type (BT) and incubation length (IL) when using in situ incubations for determination of indigestible components. Indigestible components play an integral role as an internal marker for rumen kinetics and digestibility estimations, as well as providing parameter estimates for nutritional models. However, the accuracy of internal markers is dependent upon the incubation technique utilized, with BT and IL acting as sources of error. A 3 × 2 factorial design was used to investigate the effect of BT (F57, F58, and Dracon) and IL (288 and 576 h) on indigestible dry matter (iDM) and neutral detergent fiber (iNDF) residues, as well as estimated DM and NDF digestibilities. Feed and fecal samples were obtained from two animals from an independent trial that collected total fecal production over 4 days. All bags contained a sample size-to-surface area ratio of 20 mg/cm2 and were ruminally incubated within three rumen-cannulated steers (229.5 ± 10 kg). For iDM and iNDF, feed residues demonstrated a BT × IL interaction (P < 0.01). Fecal residues for iDM and iNDF had BT and IL effects (P < 0.01) with the Dracon BT and 576 h IL having the least residues. Digestibility estimates for DM and NDF were influenced by BT × IL (P < 0.01) with the accuracy of estimates demonstrating a BT × IL interaction (P < 0.01) in which F57 at 576 h had the greatest accuracy. Resultant fecal recovery estimates for DM and NDF excreted exhibited a BT × IL interaction (P < 0.01), with F57 at 576 h better representing the excretion profiles. Based upon our analysis, utilizing the F57 BT at 576 h IL combination provides more accurate estimates relative to all other combinations used within the current study.

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Evaluation of different inclusion levels of dry live yeast impacts on various rumen parameters and in situ digestibilities of dry matter and neutral detergent fiber in growing and finishing beef cattle

Journal of Animal Science ◽

10.1093/jas/skz342 ◽

2019 ◽

Vol 97 (12) ◽

pp. 4987-4998 ◽

Cited By ~ 2

Author(s):

Caitlyn M Cagle ◽

Luiz Fernando D Batista ◽

Robin C Anderson ◽

Mozart A Fonseca ◽

Matt D Cravey ◽

...

Keyword(s):

Block Design ◽

Random Effect ◽

Gas Production ◽

Neutral Detergent Fiber ◽

Repeated Measure ◽

Beneficial Result ◽

Ch4 Concentration ◽

Live Yeast

Abstract This study evaluated the effects of supplementing dry live yeast (LY; Saccharomyces cerevisiae) on in vitro gas production (IVGP) fermentation dynamics, pH, and CH4 concentration at 48 h, and in situ rumen parameters and digestibility of DM (DMD) and NDF (NDFD) of growing cattle during 3 feeding phases: grower (GRW) for 17 d (38% steamed-flaked corn; SFC), transition (TRANS) for 15 d (55.5% SFC: 1.2 Mcal/kg NEg), and finisher (FIN) for 13 d (73% SFC: 1.23 Mcal/kg NEg). Twenty British-crossbred, ruminally cannulated steers (183 kg ± 44 kg) 6 mo of age were blocked by weight into 5 pens containing Calan gate feeders and received a control (CON) diet (17.2% CP, 35.8% NDF, 86.7% DM) without LY on days −12 to 0. After that, animals were randomly assigned to treatments (TRT), 5 animals per TRT: CON or LY at inclusion rates of 5 g/d (LY1), 10 g/d (LY2), or 15 g/d (LY3) top dressed every morning at 0800 for 45 d. The DMD and NDFD were assessed during 7 separate collection days using in situ nylon bags containing 5 g of GRW, TRANS, or FIN diets, incubated at 1200 for 48 h. Protozoa counts (PC) were determined during 5 collection periods. Data were analyzed as a repeated measure within a randomized complete block design, assuming a random effect of the pen. For GRW, TRT altered the total gas production of the nonfiber carbohydrate (NFC; P = 0.045) and the fractional rate of degradation (kd) of the fiber carbohydrate (FC) pool (P = 0.001) in a cubic pattern (P ≤ 0.05): LY2 had the most gas production and fastest kd. TRT also influenced DMD (P = 0.035) and NDFD (P = 0.012) with LY2 providing the greatest digestibility. For TRANS, TRT tended to affect the NFC kd (P = 0.078) and influenced pH (P = 0.04) and DMD (P < 0.001) in which LY2 yielded the fastest kd, highest pH, and greatest DMD. For FIN, there was an effect of TRT on total gas production (P < 0.001) and kd (P = 0.004) of the NFC pool, FC kd (P = 0.012), in vitro CH4 concentration (P < 0.001), PC (P < 0.001), DMD (P = 0.039), and NDFD (P = 0.008). LY1 had the highest PC and provided the greatest DMD and NDFD. LY2 had the fastest kd of both the NFC and FC pools and had the least CH4 concentration. LY3 had the greatest NFC gas production. No specific dose–response pattern was observed, but 10 g/d provided the most beneficial result for all diets. We concluded that supplementation with LY affected IVGP as well as ruminal parameters and digestibilities.

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Chemical characteristics and digestibility of forage maize hybrids associated with foliar fungicide application

Semina Ciências Agrárias ◽

10.5433/1679-0359.2020v41n6supl2p3285 ◽

2020 ◽

Vol 41 (6supl2) ◽

pp. 3285-3298

Author(s):

Marcelo Cruz Mendes ◽

◽

Alan Junior Stadler ◽

Kathia Szeuczuk de Oliveira ◽

Mikael Neumann ◽

...

Keyword(s):

Block Design ◽

Neutral Detergent Fiber ◽

Chemical Characteristics ◽

Forage Maize ◽

Maize Hybrids ◽

Fungicide Application ◽

Sowing Season ◽

Foliar Fungicide ◽

Dough Stage

The objective of this study was to evaluate the effect of foliar fungicide application on the chemical characteristics and in situ digestibility of forage and silage in forage maize hybrids with different grain textures in two sowing seasons. A randomized complete block design was used with four replications, in a 4 x 2 factorial arrangement, 4 hybrids, with and without foliar fungicide application, in two sowing seasons. The hybrids used were DKB240PRO, AS1572PRO, 32R48YH and AG8690PRO3. Data were tested by analysis of variance; the means were compared by Tukey’s test at 5% probability and two non-orthogonal contrasts were performed. The application of fungicide on forage maize hybrids reduced the content of neutral detergent fiber (NDF), acid detergent fiber (ADF), soft dough stage and in the silage produced. The sowing season in December negatively influenced the chemical parameters of forage maize hybrids, compared to sowing in October. Hybrids with highly vitreous endosperm presented the highest contents of NDF and ADF, soft dough stage and in the silage produced. The application of fungicide (trifloxystrobin + prothioconazole) at the V8 and R1 stages positively influenced in situ digestibility soft dough stage and in the silage produced, depending of the sowing season.

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A study of in situ degradability: heterogeneity of variances and correlated errors

Scientia Agricola ◽

10.1590/s0103-90162007000500013 ◽

2007 ◽

Vol 64 (5) ◽

pp. 548-554 ◽

Cited By ~ 8

Author(s):

Taciana Villela Savian ◽

Joel Augusto Muniz

Keyword(s):

Cynodon Dactylon ◽

Neutral Detergent Fiber ◽

Experimental Unit ◽

Correlated Errors ◽

Linear Behavior ◽

Inverse Variance ◽

Autoregressive Errors ◽

Cynodon Nlemfuensis ◽

Colonization Time

Degradation models exhibit a non-linear behavior and the selection of a model to describe the degradability depends on the coherence of the model with the involved biological events. The purpose of this study is to evaluate the behavior of the parameters of the degradation model proposed by Mertens & Loften, adjusted to the results of an in situ degradability trial. The experiment evaluated the potential degradable residue of neutral detergent fiber (NDF) of coastcross grass (Cynodon dactylon × Cynodon nlemfuensis) submitted to two cutting ages (30 and 90 days), with three replicates. For each cutting age, the potentially degradable residue of NDF was studied using fifteen incubation times (0; 0,5; 1; 3; 6; 9; 12; 18; 34; 35; 48; 56; 72; 96 e 120 hours). The experimental unit comprised one non-lactating cow with a permanent ruminal fistula. Mean and individual adjustments were obtained for the animals in three different configurations: inverse variance weight without autoregressive errors; unweighted with autoregressive errors, and unweighted without autoregressive errors. Variances of parameter estimators were also obtained by means of the mean parameter covariance matrix, providing expressions for the estimation of the confidence age for the parameters of the model. A weighting of the model by the inverse variance resulted in estimates statistically equal to zero for the colonization time. The use of a structure of second order autoregressive errors improved the fit of the model of Mertens & Loften, providing more precise estimates of the parameters.

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In situ degradability of elephant grass ensiled with acerola by-product

Semina Ciências Agrárias ◽

10.5433/1679-0359.2019v40n5supl1p2427 ◽

2019 ◽

Vol 40 (5Supl1) ◽

pp. 2427

Author(s):

Ana Cristina Holanda Ferreira ◽

Norberto Mario Rodriguez ◽

José Neuman Miranda Neiva ◽

Patrícia Guimarães Pimentel ◽

Fernando César Ferraz Lopes ◽

...

Keyword(s):

Adult Male ◽

Crude Protein ◽

Dry Matter ◽

Laboratory Analysis ◽

Neutral Detergent Fiber ◽

Pennisetum Purpureum ◽

Elephant Grass ◽

Acid Detergent Fiber ◽

Plot Design

This study evaluated the inclusion of increasing contents (0; 35; 70; 105 and 140 g kg-1) of dried acerola fruit (Malpighia glabra, Linn.) by-product (DABP) in the ensilage of elephant grass (Pennisetum purpureum, Schum.) considering the in situ degradability of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and hemicellulose. Plastic drums with 210 L capacity were used as experimental silos. After 45 days of ensiling, silos were opened and silage samples were collected for the degradability trial and laboratory analysis. For the in situ degradability trial, was used one rumen-fistulated adult male cattle. The five by-product inclusion contents were tested in three replicates, with five incubation times, in a randomized complete split-plot design in which the contents of DABP were the treatments (plots), the different silos were the replicates and the incubation times were the sub-plots. Longer incubation times significantly increased (P < 0.05) the disappearance rates of DM, CP, NDF, ADF and hemicellulose. The effective degradability of the DM, NDF and hemicellulose was higher in the silages with 35 g kg-1 of DABP in the rumen passage rates of 0.02; 0.05 and 0.08 h-1. Dried acerola fruit by-product can be added to elephant grass ensiling up to 35 g kg-1 without reducing the effective degradability of nutrients.

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Botanical Composition and In Situ Dry Matter Degradability of Legume- Grass Mixture Pasture Fertilized with Different Amounts of Nitrogen

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v6i1.16-21.1413 ◽

2018 ◽

Vol 6 (1) ◽

pp. 16 ◽

Cited By ~ 1

Author(s):

Numan Kılıçalp ◽

Mustafa Avcı ◽

Hatice Hızlı ◽

Rüştü Hatipoğlu ◽

Tugay Ayaşan

Keyword(s):

Nitrogen Fertilizer ◽

Dry Matter ◽

Positive Relationship ◽

Block Design ◽

N Fertilizer ◽

Neutral Detergent Fiber ◽

Net Energy ◽

Botanical Composition ◽

Randomized Block Design

This study was conducted to determine the effects of different nitrogen (N) fertilizer levels on botanical composition, dry matter (DM) yield, chemical composition, in situ ruminal DM and NDF degradability and net energy lactation (NEL) of a native legume-grass mixture pasture. Field and degradability trials were arranged in a Complete Randomized Block Design with three replicates. Pasture was fertilized at six levels of N fertilizer (ammonium nitrate, N0, N50, N100, N150, N200 and N250 kg/ha). Three rumen cannulated Holstein Friesian heifers (3-4 year old) were used. Feed samples were incubated for 0, 8, 12, 24, 36, 48, 72 and 96 h. Application of nitrogen fertilizer results indicated that nitrogen fertilizer decreased the legumes ratio (LR) of pasture, approximately 22.4%, Although N fertilizer increased the grass ratio (GR) of pasture approximately 55.8%. Dry matter (DM), Net energy lactation (NEL, Mcal/kg) and ash contents of pasture were increased by increasing N fertilizer level. However, when N fertilizer level increased acid detergent fiber (ADF) concentration of pasture decreased. Application of the different rate of nitrogen fertilizer had no effect on in situ rumen degradability of DM and NDF of pasture. It was also found that there was the significant positive relationship between effective NDF degradability at 48 h. rumen incubation period and different level of N fertilizer. In addition, it was determined that there was a linear and quadratic positive relationship between DM yield and different nitrogen doses. In conclusion, different rates of nitrogen application changed botanical composition, decreased ADF content and increased NEL and effective rumen degradability of neutral detergent fiber (EDNDF 48 h.) of pasture.

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174 Forage Production and Nutritive Value of Alfalfa-bermudagrass Mixtures Managed Under Contrasting Defoliation Strategies in the Southeast US

Journal of Animal Science ◽

10.1093/jas/skab235.172 ◽

2021 ◽

Vol 99 (Supplement_3) ◽

pp. 96-96

Author(s):

Liliane Severino da Silva ◽

Justin C Burt ◽

Mary K Mullenix ◽

Jennifer J Tucker

Keyword(s):

Nutritive Value ◽

Near Infrared ◽

Cynodon Dactylon ◽

Block Design ◽

Neutral Detergent Fiber ◽

Forage Production ◽

Dual Purpose ◽

Southeast Us ◽

Livestock Systems ◽

Synthetic Nitrogen Fertilizer

Abstract Incorporation of alfalfa (Medicago sativa) into bermudagrass pastures improves forage quality and decreases the reliance on synthetic nitrogen fertilizer. The objective of this study was to determine forage mass (FM), nutritive value (NV), and botanical composition of ‘Bulldog 805’ alfalfa and ‘Tifton 85’ bermudagrass (T85; Cynodon dactylon) mixtures managed under three defoliation strategies: 1) hay production (H), 2) grazing (G) or 3) dual-purpose (DP) use. The study was conducted in two locations (Headland, AL and Tifton, GA) using a randomized complete block design with two replicates. In spring 2020, grazed plots were divided in four strips and every 7-d, animals were rotated to a new strip and stocking rate was adjusted. Under DP, plots were grazed until mid-July, then forage was harvested in late August. For H plots, forage was harvested every 28 to 35-d. Forage samples were collected prior defoliation to determine FM and on grazed periods, pre- and post-grazing samples and disk meter measurements were collected. Nutritive value responses were determined using near-infrared spectroscopy. There was no effect of defoliation strategy on FM (P = 0.604; mean 3471 kg DM/ha). Greater FM (P = 0.002) was observed in July and August than June (3531 and 3976 vs 2905 kg DM ha-1, SE= 263). This response was associated with an up to 40% increase of T85 proportion in the mixture (P = 0.001). Alfalfa percentage was 78% greater for DP than G (P = 0.029). There were no differences among treatments for NV responses (P > 0.05). Among defoliation periods, crude protein, neutral detergent fiber and acid detergent fiber concentrations ranged from 14 to 22%, 45 to 58% and 28 to 35%, respectively. These preliminary results demonstrate multi-use options for this mixture in the region while aiming for increased forage nutritive value, and extended growing season, and sustainability of forage-livestock systems.

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