scholarly journals 148 The influence of kudzu (Pueraria montana var. lobata) age on in situ rumen degradation

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 38-38
Author(s):  
Joseph Gulizia ◽  
Kevin Downs ◽  
Song Cui
Keyword(s):  
Incubation Time ◽  
Repeated Measures ◽  
Dry Matter ◽  
Degradation Rate ◽  
Block Design ◽  
Neutral Detergent Fiber ◽  
Late Season ◽  
Rumen Degradation ◽  
Pueraria Montana

Abstract Early and late season kudzu (Pueraria montana var. lobata) leaves were collected to analyze dry matter (DM), crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF) in situ rumen disappearance to assess kudzu quality. Four studies were conducted during different growing seasons [2 repeated early season (ES); 2 repeated late season (LS) to determine age variability effects. Kudzu collected from 7 middle Tennessee counties were incubated in the rumen for designated lengths of time (0.25, 1, 12, 24, 36, 48, and 72 h) to determine total rumen degradation (%) and rate of disappearance (% / h). Data were analyzed as a randomized complete block design (RCBD) with repeated measures. Regression analysis was used to determine degradation rate. Overall season effects (across incubation times) for dry matter disappearance (DMD) were significantly different (P < 0.0001). Significant season by incubation time interactions were exhibited for total DMD at 12 (P < 0.0001), 24 (P = 0.0004), 36 (P = 0.0055), 48 (P = 0.0209), and 72 h (P = 0.0384) incubation, with an average rumen degradability of ES kudzu being 9.75% higher than LS between 12 and 72 h. Slope regression between 1 and 24 h determined a rumen degradation rate of 2.41% / h across both seasons, with no significant seasonal difference (P = 0.3396). These results are indicative that ES kudzu is more highly rumen degradable than LS, as evidenced by the significant season by incubation time interactions between 12 and 72 h of incubation. Although there are significant seasonal effects on kudzu DMD during certain incubation times, DMD overall for ES and LS is still indicative of high rumen degradability with an average of 81.45% at 72 h across both seasons. Season effects were minimal for NDF, ADF, and CP rumen disappearance.

scholarly journals 55 The influence of kudzu (Pueraria montana var. lobata) age on in situ rumen degradation

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 85-85
Author(s):  
Joseph Gulizia ◽  
Kevin Downs ◽  
Song Cui
Keyword(s):  
Incubation Time ◽  
Repeated Measures ◽  
Dry Matter ◽  
Degradation Rate ◽  
Block Design ◽  
Neutral Detergent Fiber ◽  
Late Season ◽  
Rumen Degradation ◽  
Pueraria Montana

Abstract Early and late season kudzu (Pueraria montana var. lobata) leaves were collected to analyze dry matter (DM), crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF) in situ rumen disappearance to assess kudzu quality. Four studies were conducted during different growing seasons [2 repeated early season (ES); 2 repeated late season (LS) to determine age variability effects. Kudzu collected from 7 middle Tennessee counties were incubated in the rumen for designated lengths of time (0.25, 1, 12, 24, 36, 48, and 72 h) to determine total rumen degradation (%) and rate of disappearance (% / h). Data were analyzed as a randomized complete block design (RCBD) with repeated measures. Regression analysis was used to determine degradation rate. Overall season effects (across incubation times) for dry matter disappearance (DMD) were significantly different (P < 0.0001). Significant season by incubation time interactions were exhibited for total DMD at 12 (P < 0.0001), 24 (P = 0.0004), 36 (P = 0.0055), 48 (P = 0.0209), and 72 h (P = 0.0384) incubation, with an average rumen degradability of ES kudzu being 9.75% higher than LS between 12 and 72 h. Slope regression between 1 and 24 h determined a rumen degradation rate of 2.41% / h across both seasons, with no significant seasonal difference (P = 0.3396). These results are indicative that ES kudzu is more highly rumen degradable than LS, as evidenced by the significant season by incubation time interactions between 12 and 72 h of incubation. Although there are significant seasonal effects on kudzu DMD during certain incubation times, DMD overall for ES and LS is still indicative of high rumen degradability with an average of 81.45% at 72 h across both seasons. Season effects were minimal for NDF, ADF, and CP rumen disappearance.

PSXI-11 Assessing in situ rumen degradability of late season kudzu (Pueraria montana var. lobata)

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 350-350
Author(s):  
J B Adkins ◽  
J P Gulizia ◽  
Kevin M Downs ◽  
S Cui
Keyword(s):  
Repeated Measures ◽  
Dry Matter ◽  
Block Design ◽  
Leguminous Plant ◽  
Native Plant ◽  
Weed Species ◽  
Invasive Weed ◽  
Indigestible Fraction ◽  
Pueraria Montana

Abstract Kudzu (Pueraria montana var. lobata) is an invasive weed species native to eastern Asia affecting much of the southeastern United States. Its broad leaves and viny growth allow it to outcompete native plant species for sunlight and nutrients. Kudzu, however, is a leguminous plant, making it a potential feedstock for ruminant species. Browsing ruminants in areas affected by kudzu could benefit animal productivity while serving to ameliorate rapid plant growth. This study specifically sought to assess the overall rumen degradability, rate of digestion, digestible fraction, and indigestible fraction using an in situ methodology. In situ rumen degradability was analyzed using four ruminally fistulated steers as individual experimental units over two repeated trials. Samples were incubated at 1, 12, 24, 36, 48, 60, and 72 h. Kudzu used in these trials was collected during September, making analysis in this study a reflection of degradability toward the end of the growing season. Data were analyzed as a randomized complete block design with repeated measures showing no significant differences between steers or trials (P &gt; 0.05). Overall degradability across all steers and times was 69.79%. Significant changes in dry matter disappearance across all steers were observed at 1, 12, and 24 h (P &lt; 0.05) with values of 33.86, 64.78, and 74.26%, respectively, and highest observed degradability at 72 h (79.55%). Incubation times between 24 and 72 h were not used in determining rate of digestion as dry matter disappearances throughout these times were not significantly different (P &gt; 0.05). Rate of digestion (kd) was determined, using linear regression, to be 1.68% ∙ h-1 along with a digestible fraction (Do) of 28.29% and indigestible fraction (U) of 22.03%. The results of this study reflect that kudzu maintains a relatively high level of rumen degradability toward seasonal senescence, making it a functional feedstock even into the cooler months.

Ruminal degradation characteristics of corn and corn–sunflower intercropped silages prepared at two stages of maturity

1992 ◽  
Vol 72 (4) ◽  
pp. 881-889 ◽  
Author(s):  
Z. Mir ◽  
P. S. Mir ◽  
S. Bittman ◽  
L. J. Fisher
Keyword(s):  
Dry Matter ◽  
Degradation Rate ◽  
Soluble Fraction ◽  
Corn Silage ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Ruminal Degradation ◽  
Whole Plants ◽  
Two Stages

The degradation characteristics of dry matter (DM), protein, neutral detergent fiber (NDF) and acid detergent fiber (ADF) of corn–sunflower intercrop silage (CSS) and monoculture corn silage (CS) prepared from whole plants, harvested at two stages of maturity, were compared using eight mature rumen-cannulated steers. The degradation characteristics were determined by incubating the silages in nylon bags for up to 72 h in the rumen of animals fed the respective silages. The degradation characteristics determined for the silages were the soluble fraction, the insoluble but degradable fractions, the rate of degradation of the degradable fractions of silage DM and protein, and the in situ disappearance of NDF and ADF after 0.5 and 72 h of incubation. The rate of particulate passage from the rumen was determined using chromium-mordanted NDF of the four silages. Values were used to estimate effectively degraded DM and protein. The rates of DM and protein degradation were highest for late-cut CSS (6.3 and 6.0% h−1, respectively) and the least for late-cut CS (2.5 and 0.8% h−1, respectively). Averaged across stages of maturity, more (P < 0.05) DM and protein were effectively degraded with CSS (57.4 and 70.1%, respectively) than with CS (48.8 and 48.7%). Degradation of NDF in early-cut CSS was lower (P < 0.05) than in CS after 72 h of incubation. ADF disappearance from all of the silages after 72 h of incubation was similar. Ruminal degradation of DM and protein in CSS was greater than in CS, which may affect efficiency of utilization of CSS. Key words: Degradation rate, effective degradability, corn silage, intercropped corn–sunflower, steers

scholarly journals Degradability study of neutral detergent fiber of coast cross (Cynodon dactylon x Cynodon nlemfunensis)

2007 ◽  
Vol 31 (4) ◽  
pp. 1184-1190 ◽  
Author(s):  
Taciana Villela Savian ◽  
Joel Augusto Muniz ◽  
Luiz Henrique de Aquino ◽  
Vera Lúcia Banys ◽  
Daniel Furtado Ferreira
Keyword(s):  
Confidence Intervals ◽  
Incubation Time ◽  
Cynodon Dactylon ◽  
Block Design ◽  
Neutral Detergent Fiber ◽  
Parameter Estimates ◽  
Degradation Model ◽  
Early Stages ◽  
Plot Design

The objective of this work was to fit the degradation model proposed by Orskov & McDonald (1979) to data of an in situ degradability trial. Neutral detergent fiber degradations (NDF) of coast cross grass (Cynodon dactylon x Cynodon nlemfunensis) were submitted to twelve cutting ages (30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 and 360 days) in a complete block design. At each cutting age, NDF degradation was investigated using nine incubation times (0, 3, 6, 12, 24, 48, 72, 96 and 120 hours) in a split-plot design, taking cutting age as main plots and incubation time as subplots. Each plot comprised a non-lactating cow with a permanent ruminal fistula. Variances of the parameter estimates were also obtained, as well as expressions for the estimation of confidence intervals for parameters in the model. A good fit of the model to the data of neutral detergent fiber degradability in the most cutting ages was found. The cutting ages of the coast cross grass influenced the degradability of different fractions, benefiting early stages. In advanced cutting ages the parameters estimates were less precise.

Review: Recent advances in evaluation of bags made from different textiles used in situ ruminal degradation

2015 ◽  
Vol 95 (4) ◽  
pp. 493-498 ◽  
Author(s):  
Tiago Neves Pereira Valente ◽  
Edenio Detmann ◽  
Cláudia Batista Sampaio
Keyword(s):  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
In Situ Techniques ◽  
Rumen Degradation ◽  
Recent Advances ◽  
Ruminal Degradation ◽  
Woven Textile ◽  
Internal Markers

Valente, T. N. P., Detmann, E. and Sampaio, C. B. 2015. Review: Recent advances in evaluation of bags made from different textiles used in situ ruminal degradation. Can. J. Anim. Sci. 95: 493–498. Textile bags are used in the laboratory to analyze the indigestible contents (internal markers) of feedstuffs after in situ ruminal incubation. Information is needed on the rate and extent of degradation in the rumen using bags made from different materials. In situ techniques have been used extensively to measure the degradation of feedstuffs in the rumen. However, in situ techniques are prone to variability. This paper reviews the effects of particle size, the material from which bags are made, pore size, tensile strength of the bags, in situ estimation of the levels of indigestible compounds [indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF), and indigestible acid detergent fiber (iADF)], rumen degradation profiles, and the use of bags made from nylon (50 µm), F57 (Ankom®), and non-woven textile (100 g m−2).

scholarly journals Soybean hulls and / or white oat grains on the ingestive behavior of confined steers

2019 ◽  
Vol 40 (5) ◽  
pp. 1925
Author(s):  
Guilherme Joner ◽  
Dari Celestino Alves Filho ◽  
Andrei Retamoso Mayer ◽  
Patrícia Machado Martini Cattelam ◽  
Camille Carijo Domingues ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Dry Matter ◽  
Block Design ◽  
Neutral Detergent Fiber ◽  
Ingestive Behavior ◽  
Raw Material ◽  
Feeding Time ◽  
Soybean Hulls ◽  
Significance Level ◽  
Significant Difference

Dry matter and neutral detergent fiber contents act as physical regulators of voluntary intake in ruminants. Therefore, different levels of these components may interfere with animal performance and require careful assessment of the used raw material. This study was conducted with the purpose to assess the effect of soybean hulls and white oat in distinct or associated uses in the concentrate diet on the ingestive behavior of cattle. Thirty-six steers with an initial mean age of 20 months and initial mean weight of 226 kg were used. The diet was composed of 50% sorghum silage and 50% concentrate based on dry matter. The tested diets were soybean hulls (concentrate fraction composed mainly of soybean hulls), white oat grains (concentrate fraction composed mainly of white oat grains), and mixture (concentrate fraction composed of equal parts of soybean hulls and white oat grains). The experimental design was a randomized block design with 12 animals per diet. The data were compared by the PROC MIXED procedure with repeated measures in time, and the means were compared by the least significant difference test at 5% significance level. Diets presented different contents of neutral detergent fiber (66, 56, and 47%) and lignin (3.2, 3.7, and 4.1%) as the percentage of white oat grain increased in the diet. The total rumination time was lower (447 minutes) for animals that received equal parts of soybean hulls and white oat grains in the diet when compared to animals receiving a diet containing soybean hulls (483 minutes) and white oat grains (495 minutes), with no difference from each other. Animals fed a diet containing soybean hulls and white oat grains showed longer feeding time, a higher number of daily meals, shorter rumination time, shorter time spent chewing, and fewer number of chews per bolus and day. This diet results in a better dry matter and neutral detergent fiber rumination efficiencies.

scholarly journals Botanical Composition and In Situ Dry Matter Degradability of Legume- Grass Mixture Pasture Fertilized with Different Amounts of Nitrogen

2018 ◽  
Vol 6 (1) ◽  
pp. 16 ◽  
Author(s):  
Numan Kılıçalp ◽  
Mustafa Avcı ◽  
Hatice Hızlı ◽  
Rüştü Hatipoğlu ◽  
Tugay Ayaşan
Keyword(s):  
Nitrogen Fertilizer ◽  
Dry Matter ◽  
Positive Relationship ◽  
Block Design ◽  
N Fertilizer ◽  
Neutral Detergent Fiber ◽  
Net Energy ◽  
Botanical Composition ◽  
Randomized Block Design

This study was conducted to determine the effects of different nitrogen (N) fertilizer levels on botanical composition, dry matter (DM) yield, chemical composition, in situ ruminal DM and NDF degradability and net energy lactation (NEL) of a native legume-grass mixture pasture. Field and degradability trials were arranged in a Complete Randomized Block Design with three replicates. Pasture was fertilized at six levels of N fertilizer (ammonium nitrate, N0, N50, N100, N150, N200 and N250 kg/ha). Three rumen cannulated Holstein Friesian heifers (3-4 year old) were used. Feed samples were incubated for 0, 8, 12, 24, 36, 48, 72 and 96 h. Application of nitrogen fertilizer results indicated that nitrogen fertilizer decreased the legumes ratio (LR) of pasture, approximately 22.4%, Although N fertilizer increased the grass ratio (GR) of pasture approximately 55.8%. Dry matter (DM), Net energy lactation (NEL, Mcal/kg) and ash contents of pasture were increased by increasing N fertilizer level. However, when N fertilizer level increased acid detergent fiber (ADF) concentration of pasture decreased. Application of the different rate of nitrogen fertilizer had no effect on in situ rumen degradability of DM and NDF of pasture. It was also found that there was the significant positive relationship between effective NDF degradability at 48 h. rumen incubation period and different level of N fertilizer. In addition, it was determined that there was a linear and quadratic positive relationship between DM yield and different nitrogen doses. In conclusion, different rates of nitrogen application changed botanical composition, decreased ADF content and increased NEL and effective rumen degradability of neutral detergent fiber (EDNDF 48 h.) of pasture.

scholarly journals Chemical composition and in situ degradability of silages from dual-purpose sorghum hybrids

2019 ◽  
Vol 40 (4) ◽  
pp. 1565
Author(s):  
Alex Lopes da Silva ◽  
Ricardo Loiola Edvan ◽  
Leilson Rocha Bezerra ◽  
Rafael Felippe Ratke ◽  
José Avelino Santos Rodrigues ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Dry Matter ◽  
Block Design ◽  
Matter Content ◽  
Neutral Detergent Fiber ◽  
Dry Matter Content ◽  
Dual Purpose ◽  
Protein Degradability

The objective was to determine chemical composition and in situ degradability for silages of dual-purpose sorghum hybrids. Twenty-five hybrids of double-purpose sorghum were investigated in a randomized block design with three replicates. Ammoniacal nitrogen (NH3-N), pH, chemical composition of the silage and in situ degradability were determined. Silage of the hybrids 9929012, 947216, 947030, 947254, 947072, 947252, 12F042066, 1141570 and 1141562 presented greater dry matter content, ranging from 402.9 to 470.7 g kg-1. The greatest crude protein content was presented by silages from the hybrids 9929036, 9929030, 12F042224, FEPAGRO19, FEPAGRO11, 9929026, 947030, 947072, 947252, 12F042226 and BRS Ponta Negra. The silage of hybrid 1141562 exhibited the lowest neutral detergent fiber content (588 g kg-1). The highest values of dry matter and crude protein degradability were observed in the silages of the hybrids 9929030, 947252, 947072, 12F042066 and 12F042226. The use of ensilage of the 9929030 and 12F042226 hybrids is recommended because these silages present a better chemical composition of dry matter, crude protein and neutral detergent fiber and better dry matter and crude protein degradability.

scholarly journals Evaluation of the Nutrient Composition, In Vitro Fermentation Characteristics, and In Situ Degradability of Amaranthus caudatus, Amaranthus cruentus, and Amaranthus hypochondriacus in Cattle

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 18
Author(s):  
Kim Margarette C. Nogoy ◽  
Jia Yu ◽  
Young Gyu Song ◽  
Shida Li ◽  
Jong-Wook Chung ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
Nutrient Composition ◽  
Neutral Detergent Fiber ◽  
Amaranthus Cruentus ◽  
Amaranthus Caudatus ◽  
Amaranthus Hypochondriacus

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.

scholarly journals Forage yield and nutritive value of Elephant grass, Italian ryegrass and spontaneous growing species mixed with forage peanut or red clover

2014 ◽  
Vol 44 (10) ◽  
pp. 1845-1852 ◽  
Author(s):  
Michelle Schalemberg Diehl ◽  
Clair Jorge Olivo ◽  
Carlos Alberto Agnolin ◽  
Ricardo Lima de Azevedo Junior ◽  
Vinícius Felipe Bratz ◽  
...  
Keyword(s):  
Repeated Measures ◽  
Nutritive Value ◽  
Red Clover ◽  
Experimental Period ◽  
Italian Ryegrass ◽  
Forage Yield ◽  
Neutral Detergent Fiber ◽  
Elephant Grass ◽  
Grazing Systems

The objective of this research was to evaluate of three grazing systems (GS) with elephant grass (EG), Italian ryegrass (IR) + spontaneous growing species (SGS); EG + IR + SGS + forage peanut (FP); and EG + IR + SGS + red clover (RC), during the winter and summer periods in rotational grazing with dairy cattle. Experimental design was completely randomized with three treatments, two replicates with repeated measures. Lactating Holstein cows receiving 1% BW-daily feed supplement with concentrate were used in the evaluation. Eight grazing cycles were performed during the experimental period. The values of pre forage mass and stocking rate were 2.52, 2.60 and 2.99 t ha-1 and 2.64, 2.77 and 3.14 animal unit ha-1, respectively for GS. Samples of forage were collected by hand-plucking technique to analyze the crude protein (CP), neutral detergent fiber (NDF), in situ dry matter digestibility (ISDMD), in situ organic matter digestibility (ISOMD) of forage present between rows of elephant grass, in the rows of elephant grass and the legumes. Higher value of CP, ISOMD and lower of NDF were observed for the grazing systems mixed with legumes forage.

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