Endolymphatic irradiation in preparation for renal transplantation: a 26-year's follow-up

OBJECTIVE: The aim of the present study was to analyze the long-term evolution of patients submitted to endolymphatic irradiation as a pre-transplant preparation. SETTING: Referral center of university hospital. DESIGN: Case-control study. MAIN OUTCOMES MEASURES: The study was designed to evaluate the incidence of rejection, kidney loss, leukopenia, infection, and graft survival in the group treated (group 1) prior to surgery, compared to a control group (group 2) composed of patients under identical clinical conditions (sex, age, type of donor, immunosuppressive therapy and time of transplant) that did not undergo treatment preparation. PATIENTS: Patients were selected from amongst transplantation candidates on a long-term waiting list, some with a high level of antibodies against panel. The control group was chosen from amongst recently transplanted patients. Patients in the treated group received lipoiodine containing 131I with specific activity ranging between 4 and 6 mCu/ml. RESULTS: A significant difference between the two groups was found with regard to the incidence of rejection crises (21.0% in group 1 and 73.6% in group 2; P= 0.003), and the maintenance dose of azathioprine (smaller in group 1; P< 0.01). As to kidney graft loss due to rejection, a tendency to significance could be identified (10.5% in group 1 and 42.1% in group 2; P= 0.063); however, the difference was not significant between the two groups in terms of reversibility of rejection episodes during the first 60 post-transplant days. CONCLUSIONS: The authors concluded that this method, besides being relatively innocuous (there was no compromising of either the thyroid gland or of gonad function and there was no increase in tumor incidence), has an extended immunosuppressive effect, and can be indicated for cadaveric renal allograft recipients, especially those showing high panel reactivity.

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A New Non-Uremic Rat Model of Long-Term Peritoneal Dialysis

Physiological Research ◽

10.33549/physiolres.931990 ◽

2011 ◽

pp. 157-164 ◽

Cited By ~ 1

Author(s):

Y.-M. PENG ◽

Z.-J. SHU ◽

L. XIAO ◽

L. SUN ◽

W.-B. TANG ◽

...

Keyword(s):

Peritoneal Dialysis ◽

Rat Model ◽

Clinical Aspects ◽

Control Group ◽

Sham Operation ◽

Significant Difference ◽

Group 2 ◽

Group 1 ◽

Glucose Group

Together with the development of peritoneal dialysis (PD), appropriate animal models play an important role in the investigation of physiological, pathophysiological and clinical aspects of PD. However, there is still not an ideal experimental PD animal model. In this study, 45 Sprague-Dawley rats were divided into three groups. Group 1 (n=15) was receiving daily peritoneal injection through the catheter connected to the abdominal cavity, using PD solution containing 3.86 % D-glucose. Group 2 (n=15) was receiving daily peritoneal injection of 0.9 % physiological saline through a catheter. Group 3 (n=15), which was subjected to sham operation, served as controls. Our results showed that WBC counts in peritoneal effluent of Group 1 were slightly higher than those of Group 2 and control group, respectively (p<0.05). However, there was no episode of infection in any group. In addition, there was no significant difference in neutrophils fractions among these three groups. Hematoxylin-eosin and Masson’s trichrome staining demonstrated a dramatic increase in thickness of the mesothelium-to-muscle layer of peritoneum exposed to high glucose (Group 1) compared to Group 2 and controls (p<0.01). These data indicated that we established a novel rat model of PD with a modified catheter insertion method. This model is more practical, easy to operate, not too expensive and it will facilitate the investigate of long-term effects of PD.

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Therapeutic trial in experimental tegumentary leishmaniasis caused by Leishmania (Leishmania) amazonensis. A comparative study between mefloquine and aminosidine

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86822000000400008 ◽

2000 ◽

Vol 33 (4) ◽

pp. 377-382 ◽

Cited By ~ 4

Author(s):

Letícia Oba Galvão ◽

Sebastião Moreira Júnior ◽

Pedro Medeiros Júnior ◽

Gleiser José Piantino Lemos ◽

Nara Fabiana Cunha ◽

...

Keyword(s):

Treated Group ◽

Leishmania Amazonensis ◽

Control Group ◽

Therapeutic Trial ◽

End Of Treatment ◽

Significant Difference ◽

Group 3 ◽

Group 2 ◽

The Right ◽

Group 1

One hundred and eighty-two male inbred C57/BL/6 mice were infected with 3 x 106 Leishmania (Leishmania) amazonensis promastigotes of the MHOM/BR/PH8 strain by means of a subcutaneous injection in the right ear. The animals were separated in three groups: 1) oral mefloquine hydrochloride treatment (16mg/kg/day/10 days), 2) intramuscular aminosidine (Paromomycin®) treatment (20mg/kg/20 days) and 3) control. Twenty six mice of each treated group were sacrificed, one at the end of treatment (nine weeks after inoculation), and one six weeks later (fifteen weeks after inoculation). Control Group animals were sacrificed at weeks six, nine and fifteen after inoculation. There was no significant difference between Group 1 (mefloquine) and Group 3 (control) subjects. Group 2 animals (aminosidine) presented the smallest differences of all, both at the end of the treatment and six weeks later. The histopato-logical parameters have shown the following findings: a) there was no significant difference between the mefloquine treated group and the control group; the group treated with aminosidine showed fewer of vacuolated macrophages than the control group, at week 9 (end of treatment). b) both at the end of treatment and six weeks later, evaluation of tissue necrosis and tissue fibrosis revealed no differences between the treated groups. It was found that six weeks after the end of treatment, mice in the control group presented significantly more severe degrees of fibrosis than mice in the other groups. It can be concluded that mefloquine showed limited therapeutic effect in this experimental model, whereas aminosidine had a significant effect. Nevertheless, neither of them resulted in cure of the lesions.

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The effect of paricalcitol on Hepatitis B immunization in hemodialysis patients

Medical Science and Discovery ◽

10.36472/msd.v7i7.393 ◽

2020 ◽

Vol 7 (7) ◽

pp. 549-553

Author(s):

Mustafa Demir ◽

Gamze İçaçan ◽

Burkay Yakar ◽

Ayhan Doğukan

Keyword(s):

Hepatitis B ◽

Risk Groups ◽

Treated Group ◽

Maintenance Hemodialysis ◽

Control Group ◽

High Morbidity ◽

Significant Difference ◽

Group 2 ◽

And Gender ◽

Group 1

Objective: Hepatitis B virus (HBV) infection has high morbidity and mortality. Therefore vaccination for HBV is crucial, especially for risk groups. In this study, we aimed to determine the effect of paricalcitol on HBV immunization in maintenance hemodialysis (HD) patients. Methods: Forty-two maintenance HD patients enrolled in the study. Group 1 was the control group who didn't receive paricalcitol treatment (n:28, control group). Group 2 was the paricalcitol treatment group for secondary hyperparathyroidism (n:14, paricalcitol group). Anti-HBs titers were measured with a three-month interval for two times. Results: The mean age of the patients in Group 1 was 58.50(18-80) years, while of the patients in Group 2 was 46.50 (23-81) years. There was no statistically significant difference between the groups in terms of age and gender (p = 0.200, p = 0.508, respectively). Baseline anti-HBs titer in the control group was 190.32 IU/L (20.18-1000), and 187.89 IU/L (38.77-1000) in the paricalcitol treated group. After 3 months of follow-up, anti-HBs titers decreased to 114.72 IU/L (13.68-1000) from 190.32 IU/L (20.18-1000) in the control group and to the 175.27 IU/L (14.25-1000) from 187.89 IU/L (38.77-1000) in the paricalcitol group. The decrease in anti-HBs titers was significant in the control group, whereas it was not significant in the paricalcitol group (P = 0.001, 0.209, respectively). Conclusion: The protective effect of paricalcitol on hepatitis B seroconversion in HD patients was observed. We think that paricalcitol may be used as an adjuvant for hepatitis B seroconversion.

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Objective assessment of stiffness in Achilles tendinopathy: a novel approach using the MyotonPRO

BMJ Open Sport & Exercise Medicine ◽

10.1136/bmjsem-2018-000446 ◽

2018 ◽

Vol 4 (1) ◽

pp. e000446 ◽

Cited By ~ 3

Author(s):

Gafin Ericson Morgan ◽

Rhodri Martin ◽

Lisa Williams ◽

Owen Pearce ◽

Keith Morris

Keyword(s):

Dynamic Stiffness ◽

Objective Assessment ◽

Weight Bearing ◽

Achilles Tendinopathy ◽

Control Group ◽

Asymptomatic Group ◽

Musculotendinous Junction ◽

Significant Difference ◽

Group 2 ◽

Group 1

ObjectivesThe aim of this study was to establish quantitative values for asymptomatic and symptomatic Achilles tendons.DesignCohort study with a single (cross-sectional) time point of patients diagnosed with unilateral Achilles tendinopathy and an asymptomatic group with comparative homogeneity.MethodsA sample of 50 participants: 25 diagnosed with symptomatic unilateral Achilles tendinopathy (AT group) and 25 with asymptomatic Achilles tendons (control group 2). The asymptomatic side of the AT group was used as a control (control group 1). Measurements at 2 cm intervals on the tendon from its insertion at the calcaneum up to the musculotendinous junction were taken non-weight bearing (NWB) and weight bearing (WB) using the MyotonPRO.ResultsThere was a significant (p<0.005) decrease in natural oscillation frequency (F) at points 2, 3 and 4 of the AT group (NWB condition) and points 2 and 3 for the WB condition. There was a significant (p<0.005) increase in logarithmic decrement (D) at points 2 and 3 signifying a decrease in elasticity. Dynamic stiffness (S) was significantly (p<0.005) reduced in the AT group at points 2 and 3 WB and point 3 WB. There was no significant difference in creep (C) observed between the symptomatic and asymptomatic tendons. There was a significant (p<0.005) increase in mechanical stress relaxation time (R) at point 2 NWB.There was a correlation between body weight and gender on tendon mechanics, with the symptomatic tendons. No significant differences were observed between the control group 1 and control group 2.ConclusionsThe MyotonPRO measured decreased stiffness over a section of the tendon corresponding clinically with Achilles tendinopathy. This may have potential in identifying risk of injury and informing rehabilitation, however further extensive research is required to generate baseline data for specific population groups monitoring variables over time. Age, gender and body mass index appear to have some bearing on the mechanical properties of the tendon but mainly in the tendinopathy group.

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Effect of Varicocelectomy on the Frequency of Nocturnal Sperm Emissions

American Journal of Men s Health ◽

10.1177/1557988315598833 ◽

2015 ◽

Vol 10 (3) ◽

pp. 250-253

Author(s):

Cengiz Kara ◽

Ozgu Aydogdu ◽

Ural Oguz ◽

Mehmet Giray Sönmez

Keyword(s):

Inguinal Hernia ◽

Depression Score ◽

Control Group ◽

Hormone Levels ◽

Significant Difference ◽

Younger Men ◽

Serum Hormone ◽

Postoperative Serum ◽

Group 2 ◽

Group 1

The aim of this study was to investigate the frequency of nocturnal sperm emissions (NSE) in varicocele patients after varicocele surgery. A total of 127 patients, 96 varicocele (Group 1) and 31 patients with inguinal hernia (Group 2) were included in this study. Mean age, laterality of surgical procedures, spermiogram results, marital status, and postoperative serum hormone levels were noted for all patients. Two groups were compared in terms of Beck depression score (BDS) and anxiety scores (AS). The frequency of NSE and libido changes in the patients during 10 days postoperatively was evaluated. The number of the patients who had NSE and increased libido were significantly higher in the varicocelectomy group when compared with the control group. No significant difference was noted between the groups in terms of BDS, AS, and serum hormone levels. No association was reported between BDS, AS, and serum hormone levels and the presence of NSE in Group 1. The incidence of NSE was higher in younger men. Increased libido was significantly associated with NSE in Group 1. Cord dissection during surgery may be a factor on increased frequency of NSEs in varicocele patients.

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What is the impact of methotrexate on liver in patients with juvenile idiopathic arthritis? Results of liver SWE performed in a single centre

Modern Rheumatology ◽

10.1093/mr/roab064 ◽

2021 ◽

Author(s):

Vildan Güngörer ◽

Mehmet Öztürk ◽

Mustafa Yasir Özlü ◽

Şükrü Arslan

Keyword(s):

Juvenile Idiopathic Arthritis ◽

Shear Wave Elastography ◽

Term Therapy ◽

Control Group ◽

Significant Difference ◽

Group 2 ◽

Long Term Therapy ◽

And Control ◽

The Impact

ABSTRACT Objectives Long-term therapy with low-dose methotrexate (MTX) is widely used in treatment of rheumatic diseases, in children. The purpose of this study was to evaluate liver elasticity in patients with juvenile idiopathic arthritis (JIA) who received MTX and compare the results with control group. Methods Liver elasticity was evaluated with shear wave elastography (SWE) technique in 25 patients aged 3–17 years who were followed up with JIA and received MTX and compared with 25 healthy controls of the same age and weight. Factors that had an effect on liver elasticity were examined. Results The mean SWE value of patients was 2.64 ± 2.13 m/s and 24.10 ± 18.50 kPa, whereas 1.83 ± 0.16 m/s and 10.09 ± 1.83 kPa in control group. There was a significant difference in liver elasticity in the patient and control groups. When the patients were evaluated as Group 1 (< 1000 mg) and Group 2 (≥ 1000 mg) according to the cumulative MTX dose, no significant difference was obtained. There was positive correlation between liver elasticity and weekly MTX dose and age. Conclusions Our study revealed that liver elasticity significantly decreased in patients who received MTX when compared with the control group. The elastography technique will be understood better over time and used safely in many areas.

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Effects of COX 1-2 Inhibitors on Prevention of Rocuronium Injection Pain: Controlled, Randomised, Double Blind Study

10.22541/au.161646222.24501995/v1 ◽

2021 ◽

Author(s):

Mehmet Sahap ◽

Handan Gulec ◽

Esra ozayar ◽

Ozlem zdemir ◽

Merve Kacan ◽

...

Keyword(s):

General Anesthesia ◽

Control Group ◽

Anesthesia Induction ◽

Movement Response ◽

Rocuronium Bromide ◽

Cox Inhibitors ◽

Significant Difference ◽

Group 2 ◽

Withdrawal Response ◽

Group 1

Purpose : Rocuronium bromide is a painful agent while general anesthesia induction. The aim of the study is to investigate the effects of cox inhibitors as a resque agent against the rocuronium pain Methodology : Sixty patients enrolled for the general anesthesia enrolled in this study. Patients were allocated into two groups (Group 1: Dexkethoprofen group, Group 2: Control group) .Pain was evaluated by during rocuronium injection, patients were scored by a scale showed below. 0;No movement response to injection 1;Mild movement response to injection 2;Hand withdrawal response to injection 3;Arm withdrawal response to injection We also evaluated the pain with 2 questions when the patient was in the recovery room.Question 1.What was the last feeling before you fall into sleep? and question 2. Did you feel any pain on your hand during medication injection for anesthesia? Results:There were differences between the groups in terms of total pain score for example in group 1 there were 16 (53%) patients who did not make any movement during rocuronium injection while there were 22 (73%) patients in group 2.There was significant difference in injection rocuronium bromide pain between group 1: dexkethoprofen group, Group 2: control group in terms of the answer to the second question.Patients felt less pain than the control group. Conclusion:The effect of cox inhibitors on rocuronium pain was seen in our study but check is also effective in reducing pain in vascular width.

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Effect of Cryotherapy & Active Stretching Together and Active Stretching Alone for Improving Hamstring Flexibility in Asymptomatic Individuals

International Journal of Health Sciences and Research ◽

10.52403/ijhsr.20210651 ◽

2021 ◽

Vol 11 (6) ◽

pp. 342-348

Author(s):

Nishat Tabassum ◽

Sanghamitra Jena

Keyword(s):

Control Group ◽

Test Results ◽

Post Intervention ◽

Study Objective ◽

Significant Difference ◽

Group 2 ◽

Hamstring Flexibility ◽

Asymptomatic Individuals ◽

Post Assessment ◽

Group 1

Study Objective: To know about the effects of cryotherapy and active stretching together and active stretching alone for improving hamstring flexibility in asymptomatic individuals. Method: 22 subjects were participated in study of the age 18 to 40. Subjects were randomly and equally assigned to static stretching and cryotherapy (group 1) and only active stretching (group 2). Subjects in each group were given stretching and cryotherapy for three weeks. Pre and post assessment of ROM was measured by the KEA, SLR and Sit and reach test. Results: After three weeks of intervention there was a significant difference between pre intervention and post intervention score in both the group but in group 1 showed significant difference between KEA, SLR and SRT variables. Conclusion: Subject who received active stretching and cryotherapy showed better improvement than the control group who received only active stretching. Hence it can be concluded that active stretching along with cryotherapy can improve hamstring flexibility than the active stretching only. Key words: Cryotherapy, flexibility, ROM, active stretching.

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Investigation of TAp63 gene Expression and Follicle Count Using Melatonin in Cisplatin-induced Ovarian Toxicity

10.21203/rs.3.rs-52313/v1 ◽

2020 ◽

Author(s):

Hacı Öztürk Şahin ◽

Mehmet Nuri Duran ◽

Fatma Sılan ◽

Ece Sılan ◽

Duygu Sıddıkoglu ◽

...

Keyword(s):

Gene Expression ◽

Female Rats ◽

Histological Evaluation ◽

Saline Control ◽

Control Group ◽

Ovarian Toxicity ◽

Significant Difference ◽

Group 3 ◽

Group 2 ◽

Group 1

Abstract Background: Premature ovarian failure is among the most important side effects of chemotherapy during reproductive period. Preserving ovarian function is gradually gaining importance during oncologic treatment. The present study aims to investigate the potential of melatonin to protect from cisplatin-induced ovarian toxicity in rats. Twenty nine female rats were divided to three groups: Saline control group (Group 1), cisplatin group (Group 2), and cisplatin+melatonin group (Group 3). While the rats in Groups 2 and 3 were administered 5 mg/kg single dose of cisplatin via intra-peritoneal (IP) route, the rats in Group 3 were started on melatonin (20 mg/kg IP) before cisplatin administration and continued during 3 consecutive days. Ovaries were removed one week after cisplatin administration in all groups. Blood samples were obtained before the rats were decapited. Histological evaluation, follicle count, and classification were performed. TAp63 mRNA expression was evaluated using mRNA extraction and real-time polymerase chain reaction (PCR) method. Serum estradiol (E2) and anti-mullerian hormone (AMH) values were measured with enzyme immune-assay technology. Results: While primordial follicles were seen to decrease in Group 2 as compared to Group 1 (p:0.023), primordial follicle count was observed to be preserved significantly in melatonin group as compared to Group 2 (p:0.047). Moreover, cisplatin-induced histo-pathological morphology was preserved in favor of normal histology in melatonin group. A significant difference was not observed between groups with regard to mean serum AMH and E2 values (p:0.102 and p:0.411, respectively). While TAp63 gene expression significantly increased in Group 2 as compared to control group (p:0.001), we did not detect a statistically significant difference in cisplatin+melatonin group, although gene expression decreased (p:0.34). Conclusion: We conclude that concurrent administration of melatonin and cisplatin may protect from ovarian damage.

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Endovascular reflux-elimination of perforating veins by RFITT and foam sclerotherapy

Phlebologie ◽

10.12687/phleb2217-1-2015 ◽

2015 ◽

Vol 44 (01) ◽

pp. 13-17

Author(s):

J. Duben ◽

J. Gatek ◽

T. Saha ◽

G. Hnatkova ◽

L. Hnatek

Keyword(s):

Varicose Veins ◽

Control Group ◽

Foam Sclerotherapy ◽

Perforating Veins ◽

Significant Difference ◽

Power Setting ◽

Group 3 ◽

Group 2 ◽

One Year ◽

Group 1

SummaryIntroduction: During the last years, many endovascular techniques have been developed in order to eliminate not only the reflux in stem veins but in perforating veins and their tributaries, too.Aim: The aim of this study was to use endo -vascular RFITT and the foam sclerotherapy for the occlusion of perforating veins as the prime source of reflux and their tributaries.Material and Methods: The Celon method was used for the thermal treatment. Polydocalon with the concentration 1% and 2% with DSS technique was used for the foam sclerotherapy. The RFITT was accomplished in 127 perforating veins in total. This group was divided into three subgroups. The first one consists of patients where only RFITT was carried out (n= 41), in the second, there were patients with RFITT realized with sclerotherapy during one session (n= 48), in the third, RFITT was completed with sclerotherapy in one month after the RFITT intervention (n= 38). The control group included perforating veins treated only with sclerotherapy (n= 81). The power setting 6W was used on the generator during the RFITT with CelonProSurge micro and 18W for usage of Celon ProCurve probe.Results: The effectiveness of the procedure in the group 1 was 8.8%, in the group two 93.7%, in the group three 92.1% and in the control group 76.5% in one year follow up. There was no significant difference between the effectiveness in groups 1, 2 and 3. The marginal difference was among all three groups with RFITT and the control group. Significant differences were in the parameter of the extinction of visible varicose veins with the reflux from perforators. The extinction was faster in group 3 than in group 2 and in the control group and the slowest was in group 1. The significant difference was observed between groups 2 and 3 compared with group 1 and the margin difference was between groups 2 and 3 compared with the control group. No significant difference was observed between groups 1 and the control group.Conclusions: All procedures are effective. The most important is the combination of RFITT and the sclerotherapy one month after thermal intervention. This is associated with a low risk of recanalization and the fastest extinction of visible varicose veins.

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