scholarly journals Histological description of the midgut and the pyloric valve of Tropidacris collaris (Stoll, 1813) (Orthopetera: Romaleidae)

2006 ◽  
Vol 66 (4) ◽  
pp. 1045-1049 ◽  
Author(s):  
V. Wanderley-Teixeira ◽  
A. A. C. Teixeira ◽  
F. M. Cunha ◽  
M. K. C. M. Costa ◽  
A. F. S. L. Veiga ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Striate Muscle ◽  
Cell Layer ◽  
Periodic Acid ◽  
Cell Types ◽  
Epithelial Layer ◽  
Analysis Method ◽  
Periodic Acid Schiff ◽  
Epithelial Cell Layer ◽  
Tropidacris Collaris

The present research describes the histology of the midgut, gastric caeca, and pyloric valve of Tropidacris collaris (Stoll, 1813), (Orthopetera: Romaleidae). We used light microscopy, staining (Gomori's trichrome and periodic acid-Schiff (PAS)), and a routine histological analysis method (hematoxilin-eosin). The insects were obtained from, and also bred in, the Laboratory of Entomology, Department of Biology, of the Rural Federal University of Pernambuco (UFRPE). The collected material was fixed in alcoholic Boüin and embedded in paraplast. The results demonstrated that the midgut wall is composed of an inner epithelial layer and two outer layers of striate muscles: one internal (circular) and the other external (longitudinal), with connective tissue between the muscle fibers. The epithelium is single-layered, with two cell types: regenerative and elongated columnar. The gastric caeca presents muscle layers similar to those of the midgut. Simple columnar epithelium lines the gastric caeca, which presents villi and projects towards the lumen. The pyloric valve is of striate muscle tissue, covered by a single epithelial-cell layer.

Some Observations upon the Cytology of the Pars Distalis of the Surgically-removed Human Pituitary

1956 ◽  
Vol s3-97 (39) ◽  
pp. 379-391
Author(s):  
C. L. FOSTER
Keyword(s):  
Periodic Acid ◽  
Cell Types ◽  
Post Mortem ◽  
Β Cells ◽  
Human Pituitary ◽  
Periodic Acid Schiff ◽  
Golgi Bodies ◽  
Lipid Inclusions ◽  
Staining Methods ◽  
Human Anterior Pituitary

Human anterior pituitary tissue that had been removed at operation and immediately fixed was examined by a number of cytological and histochemical methods and by phase contrast and electron microscopy, and compared with similar material obtained post mortem. The general histological picture of good post-mortem material (not more than 4 hours post mortem) compared well with the surgically-removed tissue. For the study of silver impregnations of the Golgi substance, however, material removed at operation was found to be greatly superior. Evidence was obtained showing that the intracellular lipid inclusions seen post mortem were not artifacts resulting from cytolytic changes. There appeared to be no relationship between these lipid bodies and the Golgi material as revealed by the Aoyama method. No unequivocal dimorphism of the Golgi bodies, correlated with α- and β-cells, such as has been reported to occur in certain other mammals, was observed. Phospholipid was present in the granules of a substantial proportion of the α-cells. It was found that most of the cells which had been designated as β-cells after the application of certain routine staining methods, and most of the Gram-positive cells, reacted positively to the Periodic acid Schiff test: these cells could therefore be regarded as true β- or mucoid cells. A method for the demonstration in frozen sections of the cell-types, together with the lipid inclusions, is described.

scholarly journals Developmental Changes of Lymphoid Tissue in the Harderian Gland of Laying Hens

2014 ◽  
Vol 37 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Pamela Bejdić ◽  
Rizah Avdić ◽  
Ljiljana Amidžić ◽  
Velida Ćutahija ◽  
Faruk Tandir ◽  
...  
Keyword(s):  
Lymphoid Tissue ◽  
Plasma Cells ◽  
Laying Hens ◽  
Periodic Acid ◽  
Harderian Gland ◽  
Cell Types ◽  
Methyl Green ◽  
Periodic Acid Schiff ◽  
Tissue Sections ◽  
Lymphoid Nodules

Abstract The Harderian gland of 110 laying hens was histologically investigated from the time of hatching to the period of 10 months of age. Tissue sections were stained with haematoxylin and eosin, periodic acid-schiff (PAS) and methyl green-pyronin technique. The research shows that lymphoid tissue is colonised by three types of cells: heterophils, lymphocytes and plasma cells. The number of these cells is directly dependent on the bird’s age. During the lifetime of the hens there gradually comes a shift in the dominance of these three cell types. Lymphoid nodules are detected only in 40-day-old chickens, while later in adult birds the Harderian gland is the organ which contains the largest number of mature plasma cells. Some plasma cells contain Russell bodies with different size and shape.

Some Secretory Products of the Invasive Stage of a Digenetic Trematode

1968 ◽  
Vol 26 ◽  
pp. 156-157
Author(s):  
Paul L. Krupa ◽  
Arya K. Bal ◽  
Gilles H. Cousineau
Keyword(s):  
Secretory Granules ◽  
Periodic Acid ◽  
Cell Types ◽  
Digenetic Trematode ◽  
Width Ratio ◽  
Uniform Density ◽  
Acid Hydrolases ◽  
Periodic Acid Schiff ◽  
Gland Cells ◽  
Secretory Products

The fine structure of various gland cells and their secretory products was studied in the invasive stage (cercaria) of the platyhelminth parasite, Cryptocotyle lingua. Secretory granules or droplets occur in several different specialized cell types, but those that we call attention to here are found in the (1) surface cytoplasmic tegument or “cuticle”, (2) ducts of cephalic (penetration) glands, and (3) epithelial lining of the “excretory bladder”.The tegumental granules appear as numerous, membrane-bounded circular or oval profiles of uniform density (Fig. 1). They are scattered more or less randomly among mitochondria and other inclusion bodies of the tegument. Some of the longer granules, with a length to width ratio of about 7 to 1, have their long axes oriented perpendicularly to the surface plasma membrane of the parasite. In cercariae tested for acid hydrolases with sodium β-glycerophosphate in Barka and Anderson's modification of Gomori's medium, clumps of reaction product appear in the vicinity of the granules and elsewhere within the tegument, but not within the granules themselves. As granules that stain with periodic acid-Schiff, they are seen in certain subsurface gland cells as well as in the tegument under the light microscope.

Cells with affinity for periodic acid – Schiff are present in the pars intermedia of Atlantic salmon

1991 ◽  
Vol 69 (12) ◽  
pp. 3105-3108 ◽  
Author(s):  
Martin P. Komourdjian ◽  
Richard L. Saunders
Keyword(s):  
Atlantic Salmon ◽  
Periodic Acid ◽  
Cell Types ◽  
Point Of View ◽  
Pars Intermedia ◽  
Periodic Acid Schiff ◽  
Distinct Cell ◽  
Two Populations ◽  
Evolutionary Point

Examination of pituitaries from two populations of Atlantic salmon (Salmo salar) parr held under natural photoperiods revealed at least two histologically distinct cell types. One type has fine granules with affinity for lead hematoxylin (PIPbH). Another type, hitherto believed to be absent in salmonids, has larger granules which are Schiff-positive (PIPAS). These cells are significantly (P < 0.05) shorter and narrower and have significantly smaller nuclei (P < 0.05) than the PIPbH type. No evidence was found for ascribing to the PIPAS cells the functions assigned to them in other species: melanophore control and reproduction. However, PIPAS granules became evident only during fall-winter, when the smolting process begins. The incidence of these cells rose and then declined sharply during this period, suggesting a possible role in some aspect of smoltification. While the role of these PIPAS cells deserves further study, their presence is in itself important from an evolutionary point of view.

Cytochemical Reactions in Cells from Leukemic Dogs

1986 ◽  
Vol 23 (2) ◽  
pp. 103-109 ◽  
Author(s):  
C. B. Grindem ◽  
J. B. Stevens ◽  
V. Perman
Keyword(s):  
Periodic Acid ◽  
Leukemic Cell ◽  
Cell Types ◽  
Lymphocytic Leukemia ◽  
Leukemic Cells ◽  
Monocytic Leukemia ◽  
Periodic Acid Schiff ◽  
Myelocytic Leukemia ◽  
Leukocyte Alkaline Phosphatase ◽  
Acetate Esterase

Leukemic cells from 17 dogs with spontaneous leukemia were stained with leukocyte alkaline phosphatase, alpha naphthyl acetate esterase with and without fluoride, peroxidase, and periodic acid-Schiff. Cytochemistry was necessary for identification or confirmation of leukemic cell type in most dogs and resulted in changing the light microscopic morphologic diagnosis in eight of 17 dogs. Leukemic cell types diagnosed were myclomonocytic leukemia in seven dogs, monocytic leukemia in five dogs, lymphocytic leukemia in four dogs, and myelocytic leukemia in one dog.

scholarly journals Pale vacuolated epithelial cells in epididymis of aflatoxin-treated mice

Reproduction ◽  
2001 ◽  
pp. 629-641 ◽  
Author(s):  
VF Agnes ◽  
MA Akbarsha
Keyword(s):  
Epithelial Cells ◽  
Aflatoxin B1 ◽  
Histological Study ◽  
Periodic Acid ◽  
Cell Types ◽  
Principal Cell ◽  
Autoimmune Response ◽  
Periodic Acid Schiff ◽  
Thin Sections ◽  
Principal Cells

The responses of the mouse epididymal epithelium to subchronic doses of aflatoxin B1 were investigated in a histological study. Either few and large or small and profuse vacuoles containing an amorphous to dense periodic acid-Schiff-positive material were observed in the epithelium of all the segments of the epididymis. Resin-embedded semi-thin sections and transmission electron microscopy indicated that these vacuoles were intracellular. The cells that contained these vacuoles were quite different in organization and electron density from the cell types already established in the epididymal epithelium and are designated as pale vacuolated epithelial cells. Owing to aflatoxin B1 toxicity, the apical membrane of some of the principal cells, either individually or in groups, disintegrated so that the principal cells released their contents into the lumen of the duct through development of a 'fistula'. Spermatozoa from the ductal lumen entered the principal cell fistula and reached the basal lamina. If extravasation of the spermatozoa via this route occurred, it would bring about an autoimmune response, leading to the formation of spermatic granulomas and the generation of anti-sperm antibodies. Extravasation of spermatozoa seems to be offset by the underlying basal cell, which is presumed to develop into a pale vacuolated epithelial cell to enclose the disintegrating principal cells and the spermatozoa arriving at the principal cell. Thus, the development of pale vacuolated epithelial cells may be a protective device preventing an autoimmune response to sperm antigens in the context of toxicant-induced degeneration of the principal cells of the epididymal epithelium.

scholarly journals 3. MORFOLOGI DAN KANDUNGAN KARBOHIDRAT KELENJAR LINGUALIS DAN SUBLINGUALIS ITIK (Anas plathyrinchos) (Morphology and Carbohydrates Content in Lingual and Sublingual Glands of Duck (Anas plathyrinchos))

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
M Jalaluddin
Keyword(s):  
Secretory Cell ◽  
Periodic Acid ◽  
Secretory Cells ◽  
Sublingual Gland ◽  
Epithelial Layer ◽  
Periodic Acid Schiff ◽  
Tubular Gland ◽  
One Year ◽  
Hematoxylin Eosin ◽  
Carbohydrate Contents

This study was aimed to find out the morphology and carbohydrates content of lingual and sub-lingual glands of duck (Anas plathyrinchos). Sample used in this study was lingual and sublingual glands of a one year old female duck. Anatomy morphology and histology of lingual and sublingual glands were observed after processed into histological preparation and stained with hematoxylin-eosin (HE) then stained further with alcian blue (AB) pH 2.5 and periodic acid Schiff (PAS) to detect carbohydrate contents. The results showed that the lingual gland of duck cannot be observed due to its location below the epithelial layer of the tongue. While the sublingual gland of duck has an elongated shape and not granulated like typically glands, located at ventral tongue (dorsoventral lingual) between os mandibulae and covered with musculus digastricus venter oral. Staining with AB pH 2.5 and PAS showed that cytoplasm of secretory cell and secrete of lingual and sublingual glands contain acidic and neutral carbohydrates which indicated a positive reaction with intensity ranging from weak (+) to strong (+++). This study concluded that the lingual and sublingual gland found in female duck is a complex tubular gland with mucous secretory cells type. ____________________________________________________________________________________________________________________

scholarly journals Morphology and histology of the olfactory organ of two African lungfishes, Protopterus amphibius and P. dolloi (Lepidosirenidae, Dipnoi)

2021 ◽  
Vol 51 (1) ◽  
Author(s):  
Hyun Tae Kim ◽  
Jong Young Park
Keyword(s):  
Alcian Blue ◽  
Periodic Acid ◽  
Cell Types ◽  
Sensory Epithelium ◽  
Olfactory Organ ◽  
Basal Cells ◽  
Periodic Acid Schiff ◽  
Multiple Cell ◽  
Hematoxylin And Eosin ◽  
Olfactory Organs

AbstractThe olfactory organs of two African lungfishes,Protopterus amphibiusandP. dolloi, were investigated using a stereo microscope and a compound light microscope and were described anatomically, histologically, and histochemically. Like other lungfishes, these species present the following general features: i) elongated olfactory chamber (OC), ii) anterior nostril at the ventral tip of the upper lip, iii) posterior nostril on the palate of the oral cavity, iv) lamellae with multiple cell types such as olfactory receptor neurons, supporting cells, basal cells, lymphatic cells, and mucous cells (MC), and vi) vomero-like epithelial crypt (VEC) made of glandular epithelium (GE) and crypt sensory epithelium. Some of these features exhibit differences between species: MCs are abundant in both the lamellar and inner walls of the OC inP. amphibiusbut occur only in lamellae inP. dolloi. On the other hand, some between feature differences are consistent across species: the GE of bothP. amphibiusandP. dolloiis strongly positive for Alcian blue (pH 2.5)-periodic acid Schiff (deep violet coloration), and positive with hematoxylin and eosin and with Masson’s trichrome (reddish-brown staining), unlike the MCs of the two species which stain dark red with both Alcian blue (pH 2.5)-periodic acid Schiff and Masson’s trichrome but respond faintly to hematoxylin and eosin. The differing abundance of MCs in the two lungfishes might reflect different degrees in aerial exposure of the olfactory organ, while the neutral and acid mucopolysaccharide-containing VEC, as indicated by staining properties of the MCs, is evolutionary evidence thatP. amphibiusandP. dolloiare the closest living relatives to tetrapods, at least in the order Dipnoi.

Ultrastructure of the Parotid Gland of the Nine-Banded Armadillo

1977 ◽  
Vol 35 ◽  
pp. 640-641
Author(s):  
J. R. Ruby
Keyword(s):  
Endoplasmic Reticulum ◽  
Parotid Gland ◽  
Periodic Acid ◽  
Acinar Cells ◽  
Duct System ◽  
Parotid Glands ◽  
Dasypus Novemcinctus ◽  
Anterior Portion ◽  
Periodic Acid Schiff ◽  
Thin Sections

Parotid glands were obtained from five adult (four male and one female) armadillos (Dasypus novemcinctus) which were perfusion-fixed. The glands were located in a position similar to that of most mammals. They extended interiorly to the anterior portion of the submandibular gland.In the light microscope, it was noted that the acini were relatively small and stained strongly positive with the periodic acid-Schiff (PAS) and alcian blue techniques, confirming the earlier results of Shackleford (1). Based on these qualities and other structural criteria, these cells have been classified as seromucous (2). The duct system was well developed. There were numerous intercalated ducts and intralobular striated ducts. The striated duct cells contained large amounts of PAS-positive substance.Thin sections revealed that the acinar cells were pyramidal in shape and contained a basally placed, slightly flattened nucleus (Fig. 1). The rough endoplasmic reticulum was also at the base of the cell.

SEM of Hepatocytes and Biliary Passages in Goldfish Liver

1977 ◽  
Vol 35 ◽  
pp. 556-557
Author(s):  
Waykin Nopanitaya ◽  
Joe W. Grisham ◽  
Johnny L. Carson
Keyword(s):  
Carassius Auratus ◽  
Cell Layer ◽  
Three Dimensional ◽  
Cell Types ◽  
Biliary System ◽  
Fish Food ◽  
Commercial Fish ◽  
Goldfish Carassius Auratus ◽  
Commercial Fish Food

An interesting feature of the goldfish liver is the morphology of the hepatic plate, which is always formed by a two-cell layer of hepatocytes. Hepatic plates of the goldfish liver contain an infrequently seen second type of cell, in the centers of plates between two hepatocytes. A TEH study by Yamamoto (1) demonstrated ultrastructural differences between hepatocytes and centrally located cells in hepatic plates; the latter were classified as ductule cells of the biliary system. None of the previous studies clearly showed a three-dimensional organization of the two cell types described. In the present investigation we utilize SEM to elucidate the arrangement of hepatocytes and bile ductular cells in intralobular plates of goldfish liver.Livers from young goldfish (Carassius auratus), about 6-10 cm, fed commercial fish food were used for this study. Hepatic samples were fixed in 4% buffered paraformaldehyde, cut into pieces, fractured, osmicated, CPD, mounted Au-Pd coated, and viewed by SEM at 17-20 kV. Our observations were confined to the ultrastructure of biliary passages within intralobular plates, ductule cells, and hepatocytes.

Sign in / Sign up

Export Citation Format

Share Document