scholarly journals Physiological and biochemical changes in Cedrela fissilis seeds during storage

Author(s):  
David da Silva ◽  
Carlos André Stuepp ◽  
Ivar Wendling ◽  
Cristiane Vieira Helm ◽  
Alessandro Camargo Angelo

Abstract: The objective of this work was to evaluate the effect of storage on the physiological quality of cedar (Cedrela fissilis) seeds, as well as to correlate the germination and vigor of the seeds with their main biochemical changes. The experiments were carried out in a completely randomized design, in a 3×5 factorial arrangement (three environments × five storage periods). Seeds were stored for 0, 135, 280, 381, and 515 days in: a humidity chamber at 5±2ºC and 80% relative humidity, a drying chamber at 20±2ºC and 60% relative humidity, and an uncontrolled environment (laboratory) at 16±10ºC and 60±25% relative humidity. In all storage periods, the content of moisture on a wet basis and the percentages of proteins, lipids, total carbohydrates, and ash were evaluated. For the viability and vigor tests, the percentage of germination and mean germination time were calculated. At sampling time, seeds showed 11.5% water content, 85.5% germination, and mean germination time of 13.5 days, and all were negatively influenced by storage period. Protein percentage showed a downward trend, while that of carbohydrates increased as the storage period was extended. Seed germination and vigor reduce drastically with storage.

2014 ◽  
Vol 36 (1) ◽  
pp. 32-39 ◽  
Author(s):  
Elizabeth Rosemeire Marques ◽  
Eduardo Fontes Araújo ◽  
Roberto Fontes Araújo ◽  
Sebastião Martins Filho ◽  
Plínio César Soares

The objective of this study was to evaluate dormancy and physiological quality of seeds of rice cultivars during storage in different environments. After harvesting, the seeds of three rice cultivars (Seleta, Curinga and Relâmpago) were dried in the sun, to reach moisture content at around 13%. Then, they were packed in paper and stored in four environments: 5 ± 2 ºC / 70 ± 5% RH, 12 ± 2 ºC / 70 ± 5% RH, 18 ± 2 ºC / 65 ± 5% RH and in uncontrolled condition of temperature and relative humidity (natural). Physiological quality was evaluated at the beginning and at 3, 6, 9 and 12 months of storage by germination test, electrical conductivity, accelerated aging and sand emergence. The experiment was conducted in split plots in a completely randomized design with three replications. Environmental factor was applied in the plots, cultivars in the subplots and storage period in subplots. Cultivar Seleta showed higher dormancy, which was surpassed during storage regardless of environment conservation. In general, seeds stored in natural environment showed lower physiological quality. Only the seeds of cultivar Seleta, regardless of the environment, maintained germination above the minimum required for commercialization until six months of storage.


2012 ◽  
Vol 12 (53) ◽  
pp. 6385-6396
Author(s):  
LA Ochieng ◽  
◽  
PW Mathenge ◽  
R Muasya ◽  

Sorghum ( Sorghum bicolor L. Moench) is an important crop that enhances food security in a region . As a food crop, sorghum is nutritious , containing starch (75- 79%) as the major component, followed by protein (6.0 -16.1%) and oil (2.1- 5.0%). Despite the crop’s versatility, it is regrettable that sorghum yields are still fairly low in Bomet District of Kenya. One of the constraints associated with low yields is the accessibility to good quality seeds by farmers . It was assumed in this study that use of low quality seed was among the factors that could be significantly affecting the low production of sorghum in the district. This study focused on the physiological quality of seed which refers to the germination capacity, viability, characteristics related to dormancy and vigour of the seed. A total of 100 farmers were interviewed and 80 seed sample s were obtained from 80 farmers. The seeds were subjected to viability and vigour quality tests. These tests included germination (as a viability test); mean germination time and electrical conductivity tests (as vigour tests). About 29% of the seed samples taken for germination test were below the laboratory standards as stipulated in the seeds and Plant Varieties Act (CAP 326). Critical electrical conductivity values and critical mean germination time values were derived during the study . In accordance with the critical values arrived at in this study, 27.5% of the seed samples showed relatively high electric al conductivity readings and 36.3% of the seed samples showed longer mean germination time. However, 11.3% of the seed samples showed high germination percentages of above 90%. Since about 29% of farmers seeds were of bad physiological quality, it was concluded that this proportion of seed can result in poor yields and hence cause food insecurity to the people of the society , and thus require s further consideration for improvement . This study recommends that further research be conducted on the genetic, physical and sanitary quality aspects of seed planted by farmers in Bomet District so that a definite conclusive statement about the quality of seed planted by farmers in the District can be made.


Author(s):  
Astiti Rahayu ◽  
Triani Hardiyati ◽  
Ponendi Hidayat

Increased productivity of cocoa needs high quality of cocoa seeds which are generally provided by certified seed gardens located far from smallholders farm, where seed delivery takes long time and may reduce physiological quality of seeds. One effort to maintain the seed quality is by treatment the seeds with polyethylene glycol6000 (PEG 6000). This study was aimed to study the interaction of PEG 6000 concentration and storage period on cocoa seed quality, and to determine the best concentration of PEG 6000 and storage period to maintain cocoa seed quality. The research was conducted in seed storage room, seed laboratory and green houseof PPPPTK, Cianjur. The experimental design used was a factorial completely randomized design and each combination treatment repeated three times. The first factor was concentration of PEG 6000 of 0%, 20%, 40%, and 60%, and the second factor was the storage period of 3 weeks, 6 weeks, 9 weeks, and 12 weeks. The results showed that concentration of PEG 60% with three weeks storage period was able to minimize the number of moldy seeds. The concentration of PEG 20%, 40%, and 60% were able to prevent seed germination in storage until six weeks. Concentration of PEG 6000 20% was able to maintain moisture content, seed germination, and germination rate.Key words:cocoa seed, storage, recalcitrant, PEG


2018 ◽  
Vol 40 (4) ◽  
pp. 362-369 ◽  
Author(s):  
Marcelo Ramos de Oliveira ◽  
Lisandro Tomas da Silva Bonome ◽  
Henrique von Hertwig Bittencourt ◽  
Edenilson Zarowni ◽  
Luan da Silva Lefchak

Abstract: The objectives of this study were to evaluate treatments with alternative products for repelling bean weevils, and to assess their influence on the physiological quality of Phaseolus vulgaris seeds. The experiment was carried out in a completely randomized design, with four replications, in a two-factorial scheme (10x4). It comprised 10 treatments: Piper nigrum, diatomaceous earth and kaolin (4 g/kg of seed); Cinnamomum zeylanicum, Citrus sinensis and Eucalyptus sp. (1% of the seed weight); Azadirachta indica oil (3 mL/kg of seed); Gastoxin (6 g/t of seed); PET container; control; and the storage times of 0, 25, 50, and 75 days. The variables seed moisture content, germination, emergence speed, mean emergence time, weight of one thousand seeds, seed infestation, and repellency were evaluated. The treatments with kaolin and A. indica had a repellent effect at first, but they lost effectiveness by the end of the storage period. The products based on C. zeylanicum and Eucalyptus sp. did not have such impact during early periods of storage, albeit repellency grew with time. On the other hand, C. sinensis and diatom-based products were repellents, both at the beginning and at the end of storage. The treatments with diatomaceous earth, kaolin, P. nigrum, C. zeylanicum, Eucalyptus sp., A. indica, and PET container all proved to be effective in reducing bean weevil infestation, without negatively impacting the physiological quality of the seeds.


Author(s):  
Dagne Tarle ◽  
Mitiku Guya ◽  
Solomon Abera ◽  
Getachew Bekele Fereja

This study was conducted to assess the effect of drying methods and pre-treatments on nutritional content and sensory quality of dried fish. The experiment was conducted in factorial arrangement of 2×3×2 with two drying methods (sun and oven drying), three fish species (tilapia, cat fish and carp) and two preservatives treatment (garlic and ginger juice) laid out in Completely Randomized Design (CRD). Fresh fillets were analysed for their nutritional value and sensory quality. The compositions of the fresh fillets were 6.50-7.59% for ash, 74.20-76.67% for protein, 8.06-9.09% for fat and 8.47-9.12% for total carbohydrates. Drying reduced the moisture contents from 74.74-75.81% to between 7.76-8.25%, making it safe for storage. The ash content changed from 7.11 to 7.34 and from 6.50% to 6.34% for cat fish and tilapia, respectively, with statistical significance whereas no change was observed in carp with 7.60% because of drying. Drying method had no difference in ash and protein contents while increase in fat from 7.75 to 9.44% and a decrease in carbohydrate from 9.37 to 8.13% were observed in sun dried samples than that of oven dried fillets. This study showed that nutritional values of dry fish did not statistically changed during storage period of 3 months.


Author(s):  
Gerusa M. Conceição ◽  
Alessandro D. Lúcio ◽  
Liliane M. Mertz-Henning ◽  
Fernando A. Henning ◽  
Manoela Beche ◽  
...  

ABSTRACT The purpose of this study was to evaluate the effect of the chemical treatment with fungicide, insecticide, micronutrient and polymer on physiological and sanitary quality of soybean seeds during storage. The treatments were arranged in a 3 x 5 x 5 factorial scheme (cultivars x seeds treatment x storage period), in completely randomized design with four replicates. Three cultivars were used: NA 4823RG, BMX TurboRR and Fundacep 62RR. The treatments were: T1: no chemical treatment, T2: fungicide, insecticide and micronutrient; T3: fungicide, insecticide, micronutrient and polymer, T4: fungicide; T5: insecticide. After the chemical treatment, the seeds were stored under environmental conditions from May to December 2012, and seed quality was evaluated at 0, 2, 4, 6 and 8 months of storage. Seeds water content and physiological quality were determined through tests of germination, accelerated aging, seedling length, seedling dry weight and sanity. The treatment with fungicides, insecticides, micronutrients and polymer did not affect seed quality over eight months of storage and promoted the control fungi associated with the seeds.


2019 ◽  
Vol 40 (6Supl2) ◽  
pp. 2961
Author(s):  
Isneider Luiz Silva ◽  
Fernando Ribeiro Teles de Camargo ◽  
Raniele Tadeu Guimarães de Souza ◽  
Itamar Rosa Teixeira ◽  
Hamilton Kikuti

To treat seed lots during the beneficiation process industrial seed treatment (TIS) has been used on seedlings. However, the actual TIS influence on the physiological quality of soybeans throughout storage is not yet known. The objective of this work was to evaluate the effects of TIS on the physiological quality of seeds of soybean cultivars during storage. Three soybean cultivars (M7110 IPRO, RR-8473RSF, M7739 IPRO) were subjected to four chemical treatment combinations (TIC-treated fungicide / insecticide): T1 - control; T2 - fungicide (Derosal Plus® - 200mL); T3 - insecticide (Cruiser ® - 500mL); T4 - a mixture of fungicide and insecticide products metalaxyl + thiabendazole + fludioxonil (MaximAdvanced - 200 mL) and thiamethoxam insecticide (Cruiser 350 FS - 500 mL). The samples were stored for a total of six months, with evaluation before storage and monthly throughout the storage period (0, 30, 60, 90, 120, 150 and 180 days). The seeds were stored in a cold chamber regulated at 12 ± 2 ºC and a relative humidity of 45± 2%. In addition to determining the water content, the following germination and vigor tests were performed: first count, seedling length, seedling dry mass and accelerated aging. A completely randomized design was used, in a 3 x 4 x 7 factorial scheme, with four replications. The data were initiallysubjected to analysis of variance, and later discriminated by the Tukey test (p < 0.05) (cultivars and chemical products) and regression (storage period). The work concludes that the use of insecticide favored the length of seedlings in cultivar RR-8473 RSF. Chemicals affected the quality and vigor of seeds in all studied cultivars. Soybean seeds can be stored with chemical treatment for commercial purposes for up to 60 days.


2019 ◽  
Vol 23 (2) ◽  
pp. 103-111
Author(s):  
Niki Rahayu ◽  
Anne Nuraini ◽  
Sumadi Sumadi

This study was aimed at determining the effect of genotype quality of Unpad sweet corn seeds on physiological quality after four months of storage. The experimental design used was a completely randomized design with a treatment factor of 16 genotypes of sweet corn with 2 replications. The data were analyzed using the F test, if there was a significant difference in Scott Knott’s test at the level of 5%. All of the seed genotypes had different qualities, so there was a significant difference in 100 grain weight parameters, germination and simultaneous growth based on ANOVA test. Seeds that had good initial physical quality are able to maintain quality until the end of storage period. After the storage period there is the best genotype, namely with code 613 which is able to maintain its physical-physiological quality until the end of storage. Based on the results of the study, it can be concluded that the genotype of sweet corn seed npad Genotype 613 (P5 x P1 (x) (3)) has good physical and physiological qualities and can be used as a candidate for hybrid elders. This is seen from the quality of seeds that meet the requirements of quality seed characters from 100 grain weight parameters and germination.PENGARUH MUTU GENOTIP BENIH JAGUNG MANIS UNPAD TERHADAP MUTU FISIOLOGISPenelitian ini bertujuan untuk mengetahui pengaruh mutu genotip benih jagung manis Unpad terhadap mutu fisiologis setelah empat bulan penyimpanan. Rancangan percobaan yang digunakan yaitu Rancangan Acak Lengkap dengan faktor perlakuan 16 genotipe jagung manis dengan 2 ulangan. Data dianalisis menggunakan uji F, apabila terdapat perbedaan yang signifikan dilakukan uji lanjut Scott Knott taraf 5 %.Semua genotip benih memiliki mutu yang berbeda, sehingga terdapat perbedaan yang signifikan pada parameter bobot 100 butir, daya berkecambah dan keserempakan tumbuh berdasarkan uji anova. Benih yang memiliki mutu fisik awal yang baik mampu mempertahankan mutu sampai periode akhir penyimpanan. Setelah periode simpan terdapat genotip terbaik yaitu dengan kode 613 yang mampu mempertahankan mutu fisik-fisiologisnya sampai akhir penyimpanan. Berdasarkan hasil penelitian dapat diambil kesimpulan bahwa genotip benih jagung manis Unpad Genotip 613 (P5 x P1 (x) (3)) memiliki mutu fisik dan fisiologis yang baik dan dapat dijadikan sebagai calon tetua hibrida. Hal ini dilihat dari kualitas benih yang memenuhi syarat karakter benih bermutu dari parameter bobot 100 butir dan daya berkecambah.


2017 ◽  
Vol 39 (4) ◽  
pp. 363-373 ◽  
Author(s):  
Jaciara de Souza Bispo ◽  
Danielle Carolina Campos da Costa ◽  
Samara Elizabeth Vieira Gomes ◽  
Gilmara Moreira de Oliveira ◽  
Janete Rodrigues Matias ◽  
...  

Abstract: Angico is a species found in several environments in Brazil, with several applications. It is used in the timber industry and mainly in folk medicine. In order to verify a variation in the biometric characteristics and the quality of seeds from different mother-plants in different harvesting years, the following variables were studied: moisture content, diameter, density, electrical conductivity, fresh and dry matter of seedlings, germination percentage and kinetics, in a completely randomized design with a 2x3 factorial arrangement (lots x size). The obtained results showed that angico seeds from different lots showed different physiological quality, possibly due to the climate variations to which mother-plants were submitted in the different years. Seed size directly interferes with seedling growth under both controlled and greenhouse conditions, and it can be used as a vigor indicator for angico seeds.


2018 ◽  
Vol 39 (2) ◽  
pp. 565 ◽  
Author(s):  
Agda Malany Forte de Oliveira ◽  
Railene Hérica Carlos Rocha ◽  
Wellinghton Alves Guedes ◽  
George Alves Dias ◽  
José Franciraldo de Lima

The number of studies on edible coatings that are used for extending the shelf life of fruits has steadily increased. For this purpose, it is necessary to choose raw materials with characteristics that maintain product quality. The objective of this study is to evaluate the effect of coatings of Chlorella sp. on the post-harvest conservation of ‘Tommy Atkins’ mango fruits. The experiment was conducted using a completely randomized design, and spray treatments (T) with 0%, 1%, 2%, 3%, or 4% of Chlorella sp. were applied to the fruits. After the treatments, the fruits were stored for 28 days (21 days at 10 °C and 42% relative humidity and 7 days at 25 °C and 42% relative humidity), and were analyzed on day 28. There was higher pulp firmness in fruits as the concentration of Chlorella sp. was increased, and the concentration of ascorbic acid was comparatively higher (22.17%) in fruits treated with 3% Chlorella sp. The percentage of soluble solids and the soluble solids/titratable acidity ratio were decreased as the coating concentrations were decreased. The use of Chlorella sp. at 1% and 2% allowed fruit maturation without compromising fruit quality attributes during the 28-day storage period.


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