scholarly journals Effects of desensitizing agents on dentinal tubule occlusion

2004 ◽  
Vol 12 (2) ◽  
pp. 144-148 ◽  
Author(s):  
César Augusto Galvão Arrais ◽  
Daniel Chi Ngai Chan ◽  
Marcelo Giannini
Keyword(s):  
Thin Layer ◽  
Sem Analysis ◽  
Clinical Aspect ◽  
Dentinal Tubule ◽  
Third Molars ◽  
Potassium Oxalate ◽  
Dentinal Tubules ◽  
Dentin Surface ◽  
Desensitizing Agents ◽  
Tubule Occlusion

The aim of this study was to evaluate the features of dentinal tubules occlusion following application of three commercially available desensitizing agents: potassium oxalate-based / Oxa-Gel (OX), HEMA and glutaraldehyde-based / Gluma Desensitizer (GD) and acidulated phosphate fluoride-based / Nupro Gel (AF). Buccal cervical areas of twenty-four extracted human third molars were smoothed and wet-polished with SiC papers and diamond pastes, in order to simulate the clinical aspect of hypersensitive dentin cervical surfaces. The teeth were randomly divided into four groups (n=6), according to the dentin surface treatments: G1: untreated; G2: OX; G3: GD; G4: AF. Specimens were fractured in the lingual-buccal direction and prepared for SEM analysis. OX promoted tubule occlusion by crystal-like deposits in the lumen of the tubules. While GL created a thin layer over the dentin surface, AF application produced precipitates that occluded the tubules. According to the SEM analysis, all desensitizing agents were able to occlude the dentinal tubules.

scholarly journals Effects of manufacturing methods of abalone gel as a desensitisation material on the closing of dentinal tubules

2020 ◽  
Vol 53 (2) ◽  
pp. 99
Author(s):  
Sri Budi Barunawati ◽  
Wayan Tunas Artama ◽  
Suparyono Saleh ◽  
Siti Sunarintyas ◽  
Yosi Bayu Murti
Keyword(s):  
Drying Methods ◽  
Dentinal Tubule ◽  
Third Molars ◽  
Dentinal Tubules ◽  
Treatment Groups ◽  
Three Samples ◽  
Significant Difference ◽  
Manufacturing Methods ◽  
Layer Chromatography ◽  
Tubule Occlusion

Background: Abalone (Haliotis varia Linnaeus) shells possess a high arginine content and are expected to be an alternative desensitisation material that is both insoluble and able to properly close dentinal tubules. Different methods of manufacturing abalone gel affect the molecular weight, hydrophilic or hydrophobic properties and protein content of the lysis. Purpose: This study aimed to determine the effects of different manufacturing methods on the dentinal tubule closure of abalone desensitisation gel. Methods: This study involved the extraction of abalone shells followed by preparative and thin-layer chromatography. The drying of the samples was carried out by the precipitation, drying, and addition methods. The research was divided into eight treatment groups, each consisting of three samples (F1, F2, F3). Each sample was applied to two study subjects’ post-extracted third molars, which were cut into disc shapes and subsequently etched with 6% citric acid. The percentage of dentinal tubule occlusion was calculated by Image J (NIH, USA) software. Data were analysed using three-way ANOVA. Results: The results showed that there were significant differences (p < 0.05) both in terms of the effects of the samples with deposition and addition on the occlusion of the dentinal tubules and in terms of the interactions between the samples with drying and addition. There was no significant difference (p > 0.05) in terms of the interactions of the samples’ three manufacturing methods. Conclusion: The manufacture of abalone gel as a desensitisation material requires a minimum of two interactions between the sample-making method and the addition, deposition, and drying methods. The best method was deposition.

scholarly journals Effect of Erosion/Abrasion Challenge on the Dentin Tubule Occlusion Using Different Desensitizing Agents

2017 ◽  
Vol 28 (2) ◽  
pp. 216-224 ◽  
Author(s):  
Gabriela D. Canali ◽  
Rodrigo N. Rached ◽  
Rui F. Mazur ◽  
Evelise M. Souza
Keyword(s):  
Repeated Measurements ◽  
Dentinal Tubule ◽  
Third Molars ◽  
Citric Acid Solution ◽  
Healthy Human ◽  
Dentin Permeability ◽  
Dentin Tubule ◽  
Before And After ◽  
Desensitizing Agents ◽  
Tubule Occlusion

The aim of the study was to evaluate dentinal tubule occlusion, measuring the dentin permeability (Lp) and using different desensitizing agents before and after abrasive/erosive challenge. Dentin discs from 42 healthy human third molars were obtained. Minimum Lp was measured after a smear layer simulation using #600 SiC paper and maximum Lp after an immersion in 0.5 M EDTA. The specimens were treated with different desensitizers: two varnishes (Clinpro XT Varnish-CV, Fluor Protector-FP), a paste (Desensibilize Nano P-NP) and a gel (Oxa Gel-OG). The Lp of each specimen was measured immediately after the desensitizers’ application. The discs were subjected to erosion/abrasion cycles for 7 days, with 0.5% citric acid solution (6x/day) and tooth brushing (3x/day). Lp was measured after the first, fourth and seventh day of the challenge. The data were analyzed by 3-way ANOVA with repeated measurements and by a Games-Howell test (α=5%). FP and CV did not show significant differences in Lp immediately after application until the 7th day (p<0.05). OG showed a significant increase in Lp after the 4th and 7th days. NP resulted in a significantly higher permeability compared to the other materials immediately after the application and after the 1st day of challenge. All the desensitizers reduced the dentin permeability immediately after application. However, only the varnishes were able to maintain the occlusive effect after the erosion/abrasion challenge.

scholarly journals Occluding Effect of Nd:YAG Laser and Different Dentin Desensitizing Agents on Human Dentinal Tubules In Vitro: A Scanning Electron Microscopy Investigation

2012 ◽  
Vol 37 (4) ◽  
pp. 340-355 ◽  
Author(s):  
LMS Al-Saud ◽  
HNA Al-Nahedh
Keyword(s):  
Nd:Yag Laser ◽  
Ethylenediaminetetraacetic Acid ◽  
In Vitro Study ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Dentin Surface ◽  
Desensitizing Agents ◽  
The One ◽  
Scanning Electron

SUMMARY Objectives This in vitro study aimed to microscopically evaluate and compare the occluding effect of the Nd:YAG laser and different dentin desensitizing agents on human dentinal tubules. Materials and Methods The Nd:YAG laser (SunLase™ 800) and four commercially available and professionally applied dentin desensitizers (Gluma® desensitizer, Tenure Quick®, Quell™ desensitizer, and VivaSens®) were investigated in this study. Sixty-four extracted intact human molars were used. Each dentin surface was divided by shallow indentation into two halves, one of which was used for treatment and the other of which served as a control. The dentin surfaces were etched to remove any smear plugs and to mimic the open dentinal tubules of sensitive dentin using 0.5 M ethylenediaminetetraacetic acid (pH 7.4) for two minutes (applied with a microbrush) and then rinsed with an air-water syringe for 30 seconds. The laser samples (n=16) were randomly divided into four groups of four samples each. These groups were the step-up technique group, the 14-day group, the one-minute group, and the two-minute group. Forty-eight samples were treated with the four tested desensitizing agents and were randomly divided into four groups (n=12/group). Each group was further subdivided into three subgroups (n=4). Samples of the first subgroup were treated for 14 days, while those of the second subgroup were treated once. Samples of the last subgroup were fractured longitudinally after a single treatment. All of the samples were then examined under a scanning electron microscope. Results The Nd:YAG laser–irradiated dentin showed reduction or complete obliteration of the dentinal tubule lumen; thus, the treatment modified the original dentinal structure. The lased dentin surface in the two-minute group showed bubble-like changes in the area of the dentinal tubules' orifices. Statistically, the two-minute group was found to have a significantly higher percentage of partially or fully occluded tubules than did the one-minute group. All of the studied desensitizing agents produced occlusion of the dentinal tubules; however, the appearance of the precipitates, the level of coverage, and the degree of dentinal occlusion varied among the tested products. Conclusion Throughout the specified period of this study, occlusion and/or narrowing of the open dentinal tubules have been successfully achieved with both treatment approaches.

scholarly journals Comparitive Evaluation of Novel Desensitising Agents on Dentinal Tubule Occlusion-A Scanning Electron Microscopic Study

2020 ◽  
pp. 11-18
Author(s):  
Dr. Madhavi Ajit Shetty ◽  
Dr. Sharad Kokate ◽  
Dr. Vibha Hegde
Keyword(s):  
Control Group ◽  
Dentinal Tubule ◽  
Tetraacetic Acid ◽  
Electron Microscopic ◽  
Dentinal Tubules ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Desensitizing Agents ◽  
Dentinal Hypersensitivity ◽  
Tubule Occlusion

Aim: To comparatively evaluate the occlusion of dentinal tubules by four novel desensitizing agents for treatment of Dentinal Hypersensitivity. Materials and Methods: The Diode Laser and three commercially available Desensitizers the NovaminR group- (SHY-NMTM), Colgate Sensitive Pro-relief, Gluma were investigated in this study. 30 extracted mandibular molars were used (n=30). They were divided into 5 groups including the control group in which no treatment was done. Teeth were ground under water-cooled trimming wheel, to prepare flat dentin surfaces. The dentin surfaces were etched to remove any smear plugs and to mimic the open dentinal tubules of sensitive dentin using 0.5 M Ethylene Diamine Tetraacetic Acid (pH 7.4) for two minutes (applied with a micro brush) and then rinsed with an air-water syringe for 30 seconds.

scholarly journals COMPARATIVE EVALUATION OF THE EFFECTIVENESS OF DESENSITIZING DENTIFRICE AND DENTAL LASER ON DENTINAL TUBULE OCCLUSION- AN INVTRO –SEM STUDY

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Shaheen C ◽  
Preadeep Shukla ◽  
Gaurav Malhotra ◽  
Prerna Kataria ◽  
Mona Dagar
Keyword(s):  
Diode Laser ◽  
Treatment Modality ◽  
Sem Analysis ◽  
Combination Group ◽  
Control Group ◽  
Dentinal Tubule ◽  
Group 13 ◽  
Partially Occluded ◽  
Dentinal Hypersensitivity ◽  
Tubule Occlusion

Background: the present study aims to evaluate whether fluoro calcium phospho silicate (Biomin or Elzenes) or in combination with Diode laser has better dentinal tubule occlusion and can be suggested as a effective treatment modality for patients with dentinal hypersensitivity. The objectives of the study were -to evaluate effectiveness of Fluoro calcium phospho silicate on dentinal tubules occlusion, to evaluate effectiveness of Fluoro calcium phospho silicate and laser and to compare its effect with Fluoro calcium phosphosilicate alone and laser on dentinal tubule occlusion, and to evaluate the effectiveness of laser on dentinal tubule occlusion. In study setting, SEM analysis was done at Sophisticated test and instrumentation centre, Cochin University of Science and Technology (CUSAT), Kerala. 120 Dentine specimens were obtained from sound premolars extracted for orthodontic purpose from the patients of age group 13 to 40 years. All the teeth were stored in 0. 2% thymol at room temperature. In our study, Fluoro calcium phospho silicate (Biomin) showed an average results of 70.37% completely occluded tubules and 23.48% of partially occluded tubules compared to 2.68% and 28.43% in the control group. Conclusion: In our invitro study the treated groups the specimens brushed with combination group (Biomin and Laser) showed the highest percentage of tubule occlusion followed by Biomin alone and then Laser. Hence from the results, it can be stated that combination of Biomin and laser can be considered as the best effective method in office treatment modality for dentinal hypersensitivity because of its ability to occlude more tubules.. Keywords: Diode laser, Biomin or Elzenes

scholarly journals “COMPARATIVE EVALUATION OF EFFECT ON DENTINAL TUBULE OCCLUSION BY THREE DIFFERENT DESENSITIZING DENTIFICES: A SEM STUDY”

2021 ◽  
Vol 5 (11) ◽  
Author(s):  
Aman Mallick ◽  
Gaurav Malhotra ◽  
Pradeep Shukla ◽  
Prerna Kataria ◽  
C. S Joshi
Keyword(s):  
Strontium Chloride ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Dentine Hypersensitivity ◽  
Study Results ◽  
Sem Study ◽  
Group 3 ◽  
Group 2 ◽  
Tubule Occlusion

Introduction: Dentine hypersensitivity (DH) occurs on exposed dentine and is dependent on the patency of dentinal tubules. This study compared the effectiveness of toothpaste containing Strontium Chloride, Novamine, Pro-Arginine in occluding dentine tubules along with comparision of those. Materials and Method: 45 freshly extracted teeth were randomly divided (15 each) into 3 groups: Group 1 15 Teeth treated with toothpaste containing Strontium Chloride. Group 2 15 Teeth treated with toothpaste containing Novamine Group 3 15 Teeth treated with toothpaste containing Pro-Arginine. Each specimen was brushed with the dentifrice slurries and examined under SCM. Result: The mean of occluded dentinal tubules on the dentin surface after brushing with three dentifrices were significant among them Pro-arginine group showed maximum occlusion. Conclusion: The present in vitro SEM study results revealed that:  All the experimental agents– Strontium chloride, novamin, Pro- Argenine were effective in occluding dentinal tubules & the percentage (%) of occluded tubules was found to be highest for Pro- Argenine as compared to the other groups. Keywords: SCM, DH

scholarly journals Effects of Dentifrices Containing Nanohydroxyapatite on Dentinal Tubule Occlusion—A Scanning Electron Microscopy and EDX Study

2020 ◽  
Vol 10 (18) ◽  
pp. 6513
Author(s):  
Emilia Bologa ◽  
Simona Stoleriu ◽  
Gianina Iovan ◽  
Cristina Angela Ghiorghe ◽  
Irina Nica ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Study Groups ◽  
Control Group ◽  
Dentinal Tubule ◽  
Human Teeth ◽  
Dentinal Tubules ◽  
Mineral Deposition ◽  
Tubule Occlusion ◽  
Scanning Electron

This in vitro study evaluated the effects of dentifrices containing nano-hydroxyapatite (n-HAp) on dentinal tubule occlusion and on mineral deposition. Dentin specimens of ten human teeth were submersed for 30 s in 40% citric acid and then randomly divided into four groups (three study groups and one control group). In the study groups, the dentin samples were exposed to three different n-HAp toothpastes: Karex (Dr. Kurt Wolff GmbH & Co. KG, Bielefeld, Germany), Biorepair Plus Sensitive (Coswell SpA, Bologna, Italy), and Dr. Wolff’s Biorepair (Dr. Kurt Wolff GmbH & Co. KG, Bielefeld, Germany); in the control group no toothpaste was applied. All of the samples were evaluated using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) analysis. In the control group all of the samples showed a frank and wide opening of the dentinal tubules, whereas in the study groups different degrees of tubule closure by mineral depositions were observed. Toothpastes containing n-HAp determined a significant occlusion of dentinal tubules and a significant increase of mineral deposition on the dentin surface. All three tested toothpastes showed similar results regarding the degree of dentinal tubule closure. Varying degrees of differences in calcium, phosphate, carbon, and oxygen ion concentrations among the three tested toothpastes were obtained.

Evaluation of Dentinal Changes Following Application of Three Different Desensitizing Agents

2017 ◽  
Vol 68 (7) ◽  
pp. 1573-1577 ◽  
Author(s):  
Simona Stoleriu ◽  
Galina Pancu ◽  
Angela Ghiorghe ◽  
Dorina Cerasella Sincar ◽  
Sorina Solomon ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Dentinal Tubule ◽  
Nacl Solution ◽  
Human Teeth ◽  
Desensitizing Agents ◽  
Strontium Acetate ◽  
Dentin Tubules ◽  
Tubule Occlusion ◽  
Scanning Electron

The aim of this in vitro study was to evaluate the effect of three desensitizing tooth pastes on the dentinal tubule occlusion. Thirty dentin discs having a thickness of 3 mm were obtained by cutting human teeth. The discs were submersed in citric acid for 30 seconds to open the dentin tubules. Then the discs were cut in two halves. In each group 10 halves were kept in 0.9% NaCl solution) and the other 10 halves were exposed to the action of one of the tested desensitizing toothpastes. The dentin samples were placed in the machine designed to simulate tooth brushing. Three commercial desensitizing toothpastes were chosen to be applied on dentin surface. The morphology of dentin samples and the level of tubule occlusion was scored using scanning electron microscope. All the three desensitizing toothpastes demonstrated significant effects on dentinal tubule occlusion. The tooth paste containing arginine and calcium carbonate as active ingredients showed the highest degree of tubule occlusion, followed by the dentifrice containing strontium acetate and sodium fluoride.

scholarly journals Dentin Tubular Occlusion with Bioactive Glass Containing Dentrifice and Gluma Desensitizer–A Comparative Sem Evaluation

2014 ◽  
Vol 02 (01) ◽  
pp. 016-021
Author(s):  
Ashu Gupta ◽  
Neha Sharma ◽  
Manmohan Bramta
Keyword(s):  
Bioactive Glass ◽  
Treatment Modalities ◽  
Tooth Surface ◽  
Strontium Chloride ◽  
Potassium Oxalate ◽  
Dentinal Tubules ◽  
Initial Application ◽  
Desensitizing Agents ◽  
Dentinal Hypersensitivity ◽  
At Home

AbstractDentinal hypersensitivity (DH) is a painful clinical condition and is characterized by pain arising from exposed dentin in response to various stimuli. Various treatment modalities are available to treat dentinal hypersensitivity which include at-home and in-office treatment. At home treatment generally consists of a variety of dentrifices containing different constituents like stannous fluoride, strontium chloride and potassium oxalate. These agents cause occlusion of dentinal tubules which decreases both dentine permeability and fluid movement thereby reducing hypersensitivity. Recently, bioactive glass (NovaMin) has been incorporated as a remineralising ingredient in dentifrice formulations for treating Dentinal Hyprsensitivity. It relieves the symptoms by precipitating hydroxycarbonate apatite (HCA) onto the tooth surface. Another combination product consisting of an aqueous solution of 5% glutaraldehyde and 35% hydroxyethyl methacrylate (Gluma desensitizer) has also been reported to be an effective desensitizing agent. Thus, the aim of the study was to evaluate and compare the tubule occluding effect of of two desensitizing agents, which are bioactive glass (Novamin), and Gluma Desensitizer. In the present study bioactive glass was found to produce more completely occluded tubules while Gluma desensitizer caused more partial occlusion on initial application. Hence, NovaMin application could be more effective in providing relief from dentinal hypersensitivity when compared with Gluma Desensitizer.

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