Animal models of ovariectomy in rats demonstrate characteristics similar to early stage osteoarthritis

Abstract Purpose The aim of our study is to analyze the model of ovariectomy (OVX) in rats reproduced histological changes of osteoarthritis (OA). Methods For the development of the research, 12 Wistar rats were used, divided into 2 equal groups: Control Group - C (n = 6) and Osteoarthritis Group - OA (n = 6). After the 6-month experimental period, all rats were sacrificed and, subsequently, the entire knee joint complex was removed without disarticulation. For the histological evaluation of the tissue, the recommendations of the International Society for Research in OA (OARSI) were used. For data processing, each evaluation was statistically treated in both groups, comparing data from group C with the group OA. Results: Through the histological evaluation of OARSI, the evolution of OA in various tissues of the joint was evaluated. Although the OA group showed noticeable differences from group C, they were not as significant. Thus, only statistically significant favors were presented in the loss of the cartilaginous matrix (OA and C, p = 0.51), considering that the changes in the loss of ECM occurred only at the depth of 0% (superficial region), but at the depth of 50% (intermediate region) and 100% depth (deep region) did not exist. Conclusion Our study demonstrated that the OVX model is a good model to discuss OA, showing histological changes similar to those found in OA, the model demonstrated to have a progressive and slow characteristic since after the OARSI evaluations, prominent evidence was found in the initial manifestations of OA.

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Flaxseed, olive and fish oil influence plasmatic lipids, lymphocyte migration and morphometry of the intestinal of Wistar rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502010000300010 ◽

2010 ◽

Vol 25 (3) ◽

pp. 275-280 ◽

Cited By ~ 12

Author(s):

Damiana Diniz Rosa ◽

Regiane Lopes de Sales ◽

Luis Fernando de Sousa Moraes ◽

Fabíola Cesário Lourenço ◽

Clóvis Andrade Neves ◽

...

Keyword(s):

Fish Oil ◽

Intestinal Mucosa ◽

Wistar Rats ◽

Density Lipoprotein ◽

Parameter Analysis ◽

Male Rats ◽

Histological Evaluation ◽

Control Group ◽

Soy Oil ◽

Lymphocyte Migration

PURPOSE: Evaluate the effect of flaxseed, olive and fish oil on the lipid profile, preservation of villosities and lymphocyte migration in the intestinal mucosa of Wistar rats. METHODS: Thirty Wistar male rats were divided into four groups, which received the AIN-93M diet, with changes only to their lipid source: flaxseed, olive, fish, and soy oil (control group). The serum was separated for the biochemical parameter analysis. A histological evaluation was performed in the ileal portion. RESULTS: The group which was fed fish oil presented lower values when compared to the other treatments for Total Cholesterol, High-density Lipoprotein Cholesterol and Triacylglycerol (p<0.05). The animals treated with fish and olive oils presented better intestinal villosities preservation. Less deposition of lymphocytes was observed in the flaxseed group (p<0.001). CONCLUSIONS: This study demonstrated that flaxseed, olive and fish oils present different responses than soy oil for the intestinal mucosa preservation and lymphocyte proliferation in Wistar rats.

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Acute and Subacute Toxicological Evaluation of the Aerial Extract ofMonsonia angustifoliaE. Mey. ex. A. Rich in Wistar Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/4952485 ◽

2016 ◽

Vol 2016 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Anthony Jide Afolayan ◽

Olubunmi Abosede Wintola ◽

Gerda Fouche

Keyword(s):

Wistar Rats ◽

Morphological Changes ◽

Plasma Concentrations ◽

Toxicity Testing ◽

Experimental Period ◽

Control Group ◽

Toxicological Evaluation ◽

Subacute Toxicity ◽

Significant Difference ◽

Dose Dependent

The acute and subacute toxicity profile of the aerial extract ofMonsonia angustifoliain Wistar rats was evaluated. The Organization for Economic Cooperation and Development (OECD) 420 guideline was adopted in the acute toxicity testing with a single oral dose of 5000 mg/kg (b.w.). For the 28-day daily oral dosing, the extract was administered at 75, 150, and 300 mg/kg b.w.; 1% ethanol in sterile distilled water was used as control. Clinical toxicity signs were subsequently evaluated. At a single dose of 5000 mg/kg b.w. the extract elicited no treatment-related signs of toxicity in the animals during the 14 days of experimental period. In the subacute toxicity, there was no significant difference in hematological, renal, and liver function indices. However, dose-dependent significant increases were observed on the plasma concentrations of white blood cell and platelet counts of the treated animals compared to the control group. While cage observations revealed no treatment-facilitated signs of toxicity, histopathological examinations of the kidneys and liver also showed no obvious lesions and morphological changes. These results suggest that the extract may be labelled and classified as safe and practically nontoxic within the doses and period of investigation in this study.

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The Osteogenic Potential of Human Nondifferentiated and Pre-differentiated Mesenchymal Stem Cells Combined with an Osteoconductive Scaffold – Early Stage Healing

Acta Medica (Hradec Kralove Czech Republic) ◽

10.14712/18059694.2017.43 ◽

2017 ◽

Vol 60 (1) ◽

pp. 12-18 ◽

Cited By ~ 4

Author(s):

Luboš Tuček ◽

Zuzana Kočí ◽

Kristýna Kárová ◽

Helena Doležalová ◽

Jakub Suchánek

Keyword(s):

Stem Cells ◽

Wistar Rats ◽

Early Stage ◽

Osteogenic Potential ◽

Control Group ◽

Positive Staining ◽

Bone Scaffold ◽

Synthetic Bone ◽

Differentiated Cells ◽

The Right

Despite the huge research into stem cells and their regenerative properties for bone healing, there are still unanswered questions including the recipient’s respond to the presence of the stem cells, the fate of stem cells inside the bone defect and the possible advantage in utilizing pre-differentiated cells. To address these problems, we used human multipotent mesenchymal stromal/stem cells (MSCs), GMP Grade, in a rat model of bone formation. In a “bioreactor concept” approach seven Wistar rats were implanted with 0.2 g of synthetic bone scaffold seeded with 2 × 106 MSCs, seven Wistar rats were implanted with 0.2 g of synthetic bone scaffold seeded with 1 × 106 predifferentiated osteoblasts and 1 × 106 pre-differentiated endothelial cells and 14 Wistar rats were implanted with 0.2 g of synthetic bone scaffold without seeded cells into an intramuscular pocket on the left side of their back. The right side of each rat was used as a control, and 0.2 g of synthetic bone scaffold was implanted into the intramuscular pocket alone. To see the early stage healing the samples were harvested 14 days after the implantation, MSCs were detected by positive DAPI and MTCO2 staining in 43% of all the samples implanted with MSCs, and no inflammation signs were present in any implanted animal. New vessels could be found in both groups implanted with MSCs, but not in the control group of animals. However, hematoxylin-eosin staining could not detect newly created bone within the implant in any of the groups. These results were in line with COLL1 staining, where we could detect positive staining only in three cases, all of which were implanted with un-differentiated MSCs. According to our findings, there were no benefits of using the pre-differentiated of MSC.

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A Nutraceutical approach to enhance Reproductive longevity and Ovarian health using Curcuma longa in wistar Rats

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00939 ◽

2021 ◽

pp. 5385-5390

Author(s):

Gowda K M Damodara ◽

S Moodithaya Shailaja ◽

R Kedilaya Vishakh ◽

Nayanatara A K ◽

Suchetha Kumari N

Keyword(s):

Body Weight ◽

Wistar Rats ◽

Curcuma Longa ◽

Histological Evaluation ◽

Control Group ◽

Adult Rats ◽

Primordial Follicles ◽

Aging Properties ◽

Sham Control Group

Aging is associated with various physiological, pathological and psychosocial alterations. This study evaluates the benefits of Curcumin by assessing reproductive aging indices and ovarian health in Wistar rats. Laboratory bred adult rats selected for the experiment. After 12 months of follow-up, the animals were grouped into Normal control rats, Sham control group, Curcumin-1(100 mg/kg body weight), Curcumin-2(200 mg/kg body weight) and Curcumin-3 (400 mg/kg body weight). For the duration of six months Curcumin dosage was administered. The experimental parameters included estrous cycle and histological evaluation of ovarian follicles. Data were analyzed using one-way ANOVA. The percentage of primordial follicles was significantly more (p<0.001) in all the groups when compared with other types of follicles. Prolonged increase (p=0.0001) in the Diestrus phase in animals treated with different dosages of Curcumin. The current study concludes that Curcumin, an active component of Curcuma longa contributes to the anti-aging properties.

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Effect of Tobacco on Knee Joint Recovery of College Students After Sports

Tobacco Regulatory Science ◽

10.18001/trs.7.4.17 ◽

2021 ◽

Vol 7 (4) ◽

pp. 400-407

Author(s):

Xiaofeng Zhang

Keyword(s):

College Students ◽

Knee Joint ◽

Protective Effect ◽

Iodoacetic Acid ◽

Control Group ◽

Joint Cartilage ◽

Blank Control Group ◽

Knee Arthritis ◽

Osteoarthritis Cartilage ◽

Histological Changes

Objectives: By analyzing the protective effect and mechanism of tobacco on knee joint cartilage in rats, this paper studies the effect of tobacco on knee joint recovery of college students after sports. Methods: Firstly, the main subunits of nAChRs were systematically studied by using the rat knee arthritis model α 7 and α 4 and β To clarify the correlation between nAChRs and the occurrence and development of OA. Then, the OA rat model prepared by iodoacetic acid was used as the experimental object to observe the protective effect of nicotine on knee osteoarthritis cartilage in rats. Results: The histological changes of rats in MIA group were obvious after operation. The results of light microscope score and Mankin's score at 15 and 30 days were significantly higher than those in con group. Of right knee cartilage in rats in MIA group α 7, α 4 and β The expression of 2 did not change significantly on the 15th day, but increased significantly on the 30th day compared with the blank control group. Conclusion: Nicotine has a protective effect on knee bone and joint cartilage and promotes the accelerated recovery of knee bone and joint after exercise.. Key words: nicotine, knee joint, cartilage, recovery after exercise.

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Evaluation of Antipsychotic Activity of Ethanolic Extract of Dhatryadi Ghrita on Wistar Rats

Current Psychopharmacologye ◽

10.2174/2211556008666191104100712 ◽

2019 ◽

Vol 8 (3) ◽

pp. 213-237

Author(s):

Rashmi S. Pal ◽

Amrita Mishra

Keyword(s):

Animal Models ◽

Wistar Rats ◽

Ethanolic Extract ◽

Conditioned Avoidance ◽

Treated Group ◽

Control Group ◽

Antipsychotic Activity ◽

Positive Symptoms Of Psychosis ◽

Extrapyramidal Motor ◽

Herbal Formulations

Objective: Herbal formulations based on plants are effective against psychosis. The effects of Dhatryadi Ghrita on Wistar rats against psychosis were investigated. Background: An increased preference nowadays is obvious towards the use of herbal drugs in the treatment of chronic ailments. Treatment of psychiatric diseases has become easier, but the extrapyramidal motor disorders are the major adverse effect exists with most of the antipsychotic drugs. Methods: For the assessment of neuroleptic activity of the ethanolic extract of Dhatryadi Ghrita, prepared with different antipsychotic animal models, three doses of the extract (100, 200 and 300 mg/kg) were used for the study with different animal models. Result: A significant reduction of amphetamine-induced stereotype and conditioned avoidance response was observed in the extract-treated animals compared to control. Minor signs of catalepsy were visible in the extract-treated group as compared to the control group. Conclusion: The study revealed that the extract may be possessing the property to alleviate the positive symptoms of Psychosis.

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ANÁLISE DOS EFEITOS DA EXPOSIÇÃO CRÔNICA ORAL AO HERBICIDA À BASE DE GLIFOSATO NA REMODELAÇÃO CARDÍACA EM RATOS

Colloquium Vitae ◽

10.5747/cv.2020.v12.n3.v305 ◽

2020 ◽

Vol 12 (3) ◽

pp. 10-15

Author(s):

Larissa Ferreira Ros ◽

Cristiane Pontes Teodozio ◽

Ana Julia Alias Costa ◽

Renata Calciolari Rossi ◽

Rejane Batista Brinholi ◽

...

Keyword(s):

Left Ventricle ◽

Right Ventricle ◽

Cardiac Remodeling ◽

Wistar Rats ◽

Experimental Period ◽

Control Group ◽

Male Adult ◽

Herbicide Glyphosate

The aim of this study was to analyze the structural cardiac remodeling of rats exposed to the herbicide glyphosate chronically. Twenty male adult Wistar rats (300-450 g) were used, divided into a control group (CG, n=10) and group of rats fed diet contaminated by glyphosate (OG), n =10) for 180 days. After the experimental period, the rats were euthanized and to evaluate structural cardiac remodeling the heart was dissected in the left ventricle (LV), atriums (ATs) and right ventricle (RV) and heavy. There was an increase in the atrium (CG= 0,084±0,22mg e OG 0,105±0,21mg, p=0,048), right ventricles (CG= 0,219±0,04mg e OG= 0,276±0,03mg, p=0,036) and left ventricles (CG= 0,950±0,05mg e OG= 1,030±0,09mg, p=0,040) in the group exposed to glyphosate. Glyphosate promoted an increase in the cardiac chambers of rats that ingested contaminated feed.

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Amygdalin and magnetic water ameliorate histological changes in liver induced by 1, 2 dimethyl hydrazine

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v40i1.150 ◽

2016 ◽

Vol 40 (1) ◽

pp. 136-139

Author(s):

Ahmed S. Farhan

Keyword(s):

Fatty Infiltration ◽

Experimental Period ◽

Control Group ◽

Cell Necrosis ◽

Histological Changes ◽

Water Group ◽

Toxic Damage ◽

Group I ◽

Ameliorative Effect ◽

Magnetic Water

This study aimed at evaluating the ameliorative effect of amygdalin and magnetic water on 1,2-dimethyl hydrazine induced toxic damage in tissues and oxidative stress in rat liver. Seven groups of ten rats each were selected for the study. Group I animals were treated as control. Group II rats received 1,2-dimethyl hydrazine (20 mg/kg body weight) injections subcutaneously once a week for 16 consecutive weeks and then kept without any treatment untill the end of the experimental period. Group III rats received amygdalin (20 mg/100 mg) daily via Oro-gastric tube. Groups IV rats were given magnetic water freely. Group V rats were given 1,2-dimethyl hydrazine + amygdalin. Group VI rat were given 1,2-dimethyl hydrazine + magnetic water Group VII rats were given 1,2-dimethyl hydrazine + amygdalin + magnetic water. Liver histological changes were studied. Degenerative changes were observed in different areas of liver tissue in 1,2-dimethyl hydrazine group, and these changes include: Fibrosis with the appearance of cell necrosis, hemorrhage, fatty infiltration and pleomorphic nuclei. While other groups showed normal appearance of the hepatic cells but some changes were observed in 1,2-dimethyl hydrazine + amygdalin + magnetic water group include: Fibrosis with the appearance of cell necrosis, hemorrhage, fatty infiltration and pleomorphic nuclei but changes in this group were less than in 1,2-dimethyl hydrazine group. In conclusion, the present results suggest that the amygdalin and magnetic water have the potential to ameliorate carcinogen 1,2-dimethyl hydrazine induced hepatotoxicity by antioxidant and antiinflammation activity.

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An Experimental Aneurysm Model: A Training Model for Neurointerventionalists

Interventional Neuroradiology ◽

10.1177/159101990601200104 ◽

2006 ◽

Vol 12 (1) ◽

pp. 17-24 ◽

Cited By ~ 6

Author(s):

I.Q. Grunwald ◽

B. Romeike ◽

R. Eymann ◽

C. Roth ◽

T. Struffert ◽

...

Keyword(s):

Animal Models ◽

Interventional Procedures ◽

Thrombus Formation ◽

Training Model ◽

Endovascular Embolization ◽

Histological Evaluation ◽

Control Group ◽

Experimental Aneurysm ◽

Occlusion Rate ◽

The Right

Reproducible animal models not only facilitate the pre-clinical assessment of aneurysm therapy but can also help in training for interventional procedures. The objective of this study was to find an animal model that can be used to test different endovascular occlusion techniques. Aneurysms in the right common carotid artery were created in 35 NZW rabbits by distal ligation and intraluminal elastase infusion. A total of 27 aneurysms were occluded by endovascular embolization with GDC-Coils. The time needed for placement of the microcatheter into the aneurysm by a professional interventionalist, a semi-professional interventionalist and a trainee was measured. The percentage of occlusion (occlusion rate) of the aneurysms was determined angiographically after embolization and again three months later, followed by a histological examination. Aneurysms of 2–6 mm size were reliably created in all 35 animals; mean size was 3.0 mm in height and 5.5 mm in diameter. Occlusion was achieved in 27 animals. Five animals from the group of 35 were initially planned as a control group with no embolization. We added to the control group one animal whose aneurysm could not be occluded endovascularly because of partial thrombosis and small size of the aneurysm. The angiographically determined mean occlusion rate was 89.5% ± 11.3% standard deviation. Histological evaluation of the six aneurysms of the control group showed that they remained patent. Aneurysms that underwent embolization showed organized thrombus formation with no signs of recanalization. Two animals died from anaesthesia related or embolic complications. The time needed by the professional did not significantly decrease, after a little practice the trainee was nearly as quick as the professional. The beginner showed extensive progress, reducing the time for catheter placement by more than 50%. This paper describes the angiographic and histopathologic findings and also demonstrates possible methods for training in interventional procedures. Animal models will play a vital part in the training of future interventionalists. This model has the capability of testing different embolization devices (GDC, Onyx®) and methods. Histologic long-term prognosis and the physical effect of the coils can be evaluated with this model.

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Efficacy of extracellular vesicles from mesenchymal stem cells on osteoarthritis in animal models: a systematic review and meta-analysis

Nanomedicine ◽

10.2217/nnm-2021-0047 ◽

2021 ◽

Author(s):

Bocheng Zhang ◽

Xiaoyuan Tian ◽

Zhenan Qu ◽

Jiaming Liu ◽

Liang Yang ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Animal Models ◽

Extracellular Vesicles ◽

Early Stage ◽

Meta Analysis ◽

Included Study ◽

Histological Evaluation ◽

Eligibility Criteria

Background: Some studies have reported results from the use of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) to treat osteoarthritis (OA). Objective: To evaluate the efficacy of MSC-EVs as a treatment for OA. Data sources: Databases were searched using the terms ‘mesenchymal stem cells’, ‘osteoarthritis’ and ‘extracellular vesicles.’ Study eligibility criteria: Studies performed in animal models utilizing MSC-EVs to treat OA that described the macroscopic evaluation or histological evaluation were included. Study appraisal: The quality of the studies was examined using the CAMARADES quality checklist. Results: MSC-EVs were superior to the placebo in the macroscopic evaluation and histological evaluation. MSC-EVs were more effective in the early stage of OA and once a week was better than multiple times a week. Limitations: The included studies were highly heterogeneous. Conclusion: MSC-EVs may improve the results of macroscopic and histological evaluations of OA.

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