Free Radical Scavenging Properties and Induction of Apoptotic Effects of FA Fraction Obtained after Proteolysis of Bioactive Peptides from Microalgae Synechococcus sp. VDW

This study aims to determine the antioxidant activity of bioactive peptides derived from Synechococcus sp. VDW cells cultured for 21 days. Synechococcus sp. VDW protein hydrolysates were prepared with trypsin and purified by ultrafiltration with molecular mass cut-off membranes of 10, 5 and 3 kDa. The M<3 kDa (FA) fraction had the highest 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, with IC50 values of (11.5±0.3) and (13.6±0.2) µg/mL, respectively. The FA fraction was separated by reversed phase HPLC to yield four subfractions (F1–4). The F4 subfraction showed the highest maximum ABTS radical scavenging activity (3.55±0.61) % and it was selected for further analysis by electrospray ionisation quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS/MS) based on de novo peptide sequencing. Five antioxidant peptides were identified, of which AILESYSAGKTK had the highest ABTS radical scavenging activity. Furthermore, the FA fraction showed high cytotoxic activities against human cancer-derived cell lines, especially the colon cancer cell line (SW620) with an IC50 value of (106.6±21.5) µg/mL, but not the untransformed Wi38 cell line. The FA fraction activated the apoptotic pathway in SW620 cells after treatment for 24, 48 and 72 h, with the highest activities of caspases-3, -8 and -9 being observed after treatment for 72 h. These findings suggested that microalgae Synechococcus sp. VDW may be used to develop natural anticancer drugs.

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Characterization of Protein Hydrolysates from Fish Discards and By-Products from the North-West Spain Fishing Fleet as Potential Sources of Bioactive Peptides

Marine Drugs ◽

10.3390/md19060338 ◽

2021 ◽

Vol 19 (6) ◽

pp. 338

Author(s):

Andreia Henriques ◽

José A. Vázquez ◽

Jesus Valcarcel ◽

Rogério Mendes ◽

Narcisa M. Bandarra ◽

...

Keyword(s):

Inhibitory Activity ◽

Bioactive Peptides ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Protein Hydrolysates ◽

Scavenging Activity ◽

North West ◽

Chelating Activity ◽

The North ◽

By Products

Fish discards and by-products can be transformed into high value-added products such as fish protein hydrolysates (FPH) containing bioactive peptides. Protein hydrolysates were prepared from different parts (whole fish, skin and head) of several discarded species of the North-West Spain fishing fleet using Alcalase. All hydrolysates had moisture and ash contents lower than 10% and 15%, respectively. The fat content of FPH varied between 1.5% and 9.4% and had high protein content (69.8–76.6%). The amino acids profiles of FPH are quite similar and the most abundant amino acids were glutamic and aspartic acids. All FPH exhibited antioxidant activity and those obtained from Atlantic horse mackerel heads presented the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and Cu2+ chelating activity. On the other hand, hydrolysates from gurnard heads showed the highest ABTS radical scavenging activity and Fe2+ chelating activity. In what concerns the α-amylase inhibitory activity, the IC50 values recorded for FPH ranged between 5.70 and 84.37 mg/mL for blue whiting heads and whole Atlantic horse mackerel, respectively. α-Glucosidase inhibitory activity of FPH was relatively low but all FPH had high Angiotensin Converting Enzyme (ACE) inhibitory activity. Considering the biological activities, these FPH are potential natural additives for functional foods or nutraceuticals.

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Behaviour of 9-Ethyl-9H-carbazole Hydrazone Derivatives Against Oxidant Systems

Croatica Chemica Acta ◽

10.5562/cca3481 ◽

2019 ◽

Vol 92 (1) ◽

pp. 87-94 ◽

Cited By ~ 2

Author(s):

Cigdem Karaaslan ◽

Hande Gurer-Orhan ◽

Sibel Suzen ◽

Luciano Saso ◽

Omidreza Firuzi ◽

...

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Amyloid Β ◽

In Vitro Assays ◽

Cytotoxic Activities ◽

Scavenging Activity ◽

Neuroprotective Effects ◽

Neuronal Pc12 Cells

Antioxidants are helpful in prevention of several diseases related with oxidative stress including neurodegenerative disorders. In recent studies, carbazoles were given proof of promising antioxidant activities. In this article, 9-ethyl-9H-carbazole hydrazone derivatives were synthesized, characterized and their in vitro antioxidant activity and possible cytotoxic effects were investigated. Furthermore, protective effect of the synthesized derivatives against amyloid β-induced damage in PC12 neuronal cells was examined by using MTT assay. The newly synthesized carbazoles were found to have radical scavenging activity with a varying potency both in cell-free and cell-based in vitro assays. Several compounds, especially such as 3d and 3e, 3m and 3n bearing two halogen groups on the phenyl ring, were found to have cytotoxic activity. However, their cytotoxic activities were not higher than that of melatonin. Several compounds also significantly protected neuronal PC12 cells against amyloid β-induced damage, which can be defined as neuroprotective agents. (4-(2-((9-Ethyl-9H-carbazol-3-yl)methylene)hydrazinyl)benzonitrile) 3r was found as the most active compound with both radical scavenging activity and neuroprotective effects against amyloid β-induced damage. These findings might provide an alternative strategy for developing novel carbazole derivatives for management of neurodegenerative diseases, such as Alzheimer's disease.

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Antiradical Activities and Cytotoxic Effects of Phlomis Brevibracteata Turrill on SK-HEP-1 Adenocarcinoma Cell Line

Proceedings ◽

10.3390/proceedings2251575 ◽

2018 ◽

Vol 2 (25) ◽

pp. 1575

Author(s):

Imge Kunter ◽

Niloofar Zabib ◽

Kelly Randmae ◽

Mahshid Dabeshlim ◽

Muberra Kosar

Keyword(s):

Cell Line ◽

Mtt Assay ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Scavenging Activity ◽

Endemic Plant ◽

Cytotoxic Effects ◽

Phenolic Extract

The present study investigates the total phenol amount, radical scavenging and anticancer activity of methanolic extract of Phlomis brevibracteata Turrill (PBT) leaves; an endemic plant of North Cyprus. The antiradical activity and cytotoxicity of 70% methanolic extract of PBT leaves were analyzed by the implementation of DPPH, ABTS radical scavenging activity tests and MTT assay, respectively. Our results showed significant antiradical scavenging activity in the total phenolic content of the PBT leaves. MTT assay proved potent cytotoxic activity against SK-HEP-1 cell line not only for 70% methanolic extract of PBT, but also for gallic acid and caffeic acid constituents of the total phenolic extract.

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Chemotherapeutic potiential of Lupeol and its analogue (oxime) on human mammary cancer cell line (MCF-7)

Progress in Bioscience and Bioengineering ◽

10.29269/pbb2017.v1i1.3 ◽

2017 ◽

Vol 1 (1) ◽

Author(s):

Jayasri Gunasekaran ◽

Mirunalini Sankaran

Keyword(s):

Cell Line ◽

Mammary Cancer ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Cancer Cell Line ◽

Scavenging Activity ◽

Mammary Cancer Cell ◽

Underlying Mechanisms ◽

Mcf 7 ◽

Human Mammary Cancer

Lupeol, a dietary triterpene, present in many fruits and medicinal plants, has been reported to possess many pharmacological properties including anti-cancer activities both in vitro.The present study is conducted to investigate the anticancer activity and the underlying mechanisms of Lupeol analogue (oxime) on human mammary cancer (MCF-7).MTT assay showed that the lupeol analogue had marked cytotoxic activity in MCF-7 cancer cells.We observed increased levels of Antioxidants (ABTS, DPPH,Superoxide anions radical scavenging activity,Hydroxyl radical scavenging activity) in MCF-7 cell line, because of anti oxidant mechanism of lupeol analogue (oxime) .We observed the higher concentrations ( 20,30,40,50 and 70 µM/mL) of lupeol analogue treatment significantly showed increased cytotoxicity in MCF-7 cells. The IC50 value was found to be 40 µM/mL of lupeol analogue (oxime) could greatly inhibit the cell growth. So, we have chosen at 40 and 320 µM/mL concentration of lupeol analogue (oxime) for further experiments. We observed increased levels of Lipid peroxidation and decreased levels of Antioxidants (SOD,CAT,GPx,GSH) in MCF-7 cell line because of the pro oxidant mechanism of lupeol analogue (oxime) . These observations revealed the cytotoxic potential of lupeol, which could be attributed to their prooxidant property on the MCF-7 cells. It is evident from the observation made in the present study that the lupeol analogue (oxime)has potential anticancer effect compared to Lupeol.

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Development of Thai Gac Fruit Extraction as a Multifunctional Cosmeceutical Ingredient for Antioxidant, Melanogenesis and Collagen Stimulating Activities

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.819.104 ◽

2019 ◽

Vol 819 ◽

pp. 104-110

Author(s):

Sareeya Reungpatthanaphong ◽

Khanittha Chawananorasest ◽

Thanagon Kirdin ◽

Monchanok Bamrungchai ◽

Paiboon Reungpatthanaphong

Keyword(s):

Antioxidant Activity ◽

Cell Line ◽

Active Ingredient ◽

Human Fibroblast ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Fibroblast Cell ◽

Scavenging Activity ◽

Antioxidant Power ◽

Human Fibroblast Cell

Gac fruit (Momordica cochinchinensis Spreng) is mostly found in southeast Asian countries. Previous studies have demonstrated that gac fruit is a major source of β-carotene, lycopene, and α-tocopherol. This study aimed to develop the extraction of Thai gac fruit as a potential active ingredient for antioxidant, melanogenesis, and collagen stimulating activities. In the present study, different parts of ripe Thai gac fruit, the peel, pulp, and seed membrane, were extracted using the accelerated solvent extraction (ASE) technique, an innovative plant extraction method. DPPH radical-scavenging activity, ABTS radical scavenging activity, and ferric reducing/antioxidant power (FRAP) assay were performed to compare the antioxidant activity derived from each part of the fruit. The effect of Thai gac fruit on melanogenesis and collagen stimulating activities was investigated using B16F1 and human fibroblast cell lines, respectively. The results demonstrate that the pulp (EC50 value of 7.42 ± 0.81 mg/mL) and the seed membrane (EC50 value of 76.43 ± 2.66 μg/mL) showed the strongest antioxidant activities in DPPH and ABTS assay, respectively. The peel clearly displayed the strongest antioxidant activity and significantly increased the FRAP value (247.67 ± 19.73 μM/mg). Furthermore, the peel showed the lowest melanin content (76.56 ± 7.13 % of control) in the B16F1 cell line, and clearly increased the most amount of collagen (21.87 ± 3.12%) in the human fibroblast cell line in comparison to the other fractions. Therefore, Thai gac fruit peel revealed the biological effects, including antioxidant, melanogenesis, and collagen stimulating activities. In conclusion, Thai gac fruit extracted using the ASE technique exhibited several effects that could be used as a potential active ingredient for cosmeceutical products, due to its antioxidant, melanogenesis, and collagen stimulating activities. Our findings provide potential uses of Thai gac fruit as a multifunctional cosmeceutical ingredient to be further explored in future studies.

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Biological Evaluation of Curcumin and Related Diarylheptanoids

Zeitschrift für Naturforschung C ◽

10.1515/znc-2006-9-1002 ◽

2006 ◽

Vol 61 (9-10) ◽

pp. 625-631 ◽

Cited By ~ 15

Author(s):

Faridah Abas ◽

Lim Siang Hui ◽

Syahida Ahmad ◽

Johnson Stanslas ◽

D. A. Israf ◽

...

Keyword(s):

Free Radical ◽

Cell Line ◽

Cancer Cell ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Cancer Cell Line ◽

Biological Evaluation ◽

Free Radical Scavenging ◽

Lung Cancer Cell Line ◽

Cytotoxic Activities

AbstractNine derivatives of three natural diarylheptanoids, curcumin, demethoxycurcumin and bisdemethoxycurcumin, were prepared. Their antioxidant, free radical scavenging, nitric oxide (NO) inhibitory and cytotoxic activities were evaluated and compared with those of the respective natural compounds. Curcumin (1), demethoxycurcumin (2), demethyldemethoxycurcumin (C3), diacetyldemethoxycurcumin (AC2) and triacetyldemethylcurcumin (AC5) exhibited higher antioxidant activity than quercetin while products from demethylation of 1 and 2 exhibited higher free radical scavenging activity. Compounds AC2 and AC5 were found to be most active in inhibiting breast cancer cells (MCF-7) proliferation with IC50 values of 6.7 and 3.6 μм, respectively. The activity of AC2 is almost doubled and of AC5 almost tripled as compared to curcumin. Their selectivity towards different cell lines is also more noticeable. Compounds AC2 and AC5 also showed increased activity against a human prostate cancer cell line (DU-145) and non-small lung cancer cell line (NCI-H460) with IC50 values of 20.4, 16.3 and 18.3, 10.7 μм, respectively.

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The isolation, analytical characterization by HPLC-UV and NMR spectroscopy, cytotoxic and antioxidant activities of baeomycesic acid from Thamnolia vermicularis var. subuliformis

Hemijska industrija ◽

10.2298/hemind110414035m ◽

2011 ◽

Vol 65 (5) ◽

pp. 591-598

Author(s):

Nedeljko Manojlovic ◽

Perica Vasiljevic ◽

Dragan Nikolic ◽

Gordana Bogdanovic-Dusanovic ◽

Zoran Markovic ◽

...

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Herbal Medicines ◽

Rapid Identification ◽

Cytotoxic Activities ◽

Spectroscopic Methods ◽

Scavenging Activity ◽

Analytical Characterization

The aim of this work was the analytical characterization of the ?-orcinol depside, baeomycesic acid in lichens extracts. The extract of Thamnolia vermicularis var. subuliformis was analyzed by the two different methods, namely HPLC-UV and 1H NMR analysis. The results showed that baeomycesic acid was the most abundant depside in the lichens. These results could be of use for rapid identification of this metabolite in other lichen species. Besides baeomycesic acid, three depsides and one monocyclic phenolic compound were isolated from the lichen extract on the chromatographic column. The structure of baeomycesic acid was confirmed by HPLC-UV and spectroscopic methods. In addition, antioxidant and cytotoxic activities of baeomycesic acid were determined. The result of the testing showed that baeomycesic acid exhibited a moderate radical scavenging activity (IC50 = 602.10 ? 0.54 ?g/mL) and good cytotoxic activity. This is the first report of detailed analytical characterization, isolation, as well as antioxidant and cytotoxic activities of baeomycesic acid from Thamnolia vermicularis. These results may be helpful in future industrial production of herbal medicines that include this important natural product.

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Antioxidant Activities of Artemisia Absinthium and Citrus Paradisi Extracts Repress Viability of Aggressive Liver Cancer Cell Line

10.21203/rs.3.rs-641615/v1 ◽

2021 ◽

Author(s):

Muhammad Ali ◽

Rida Iqbal ◽

Muhammad Safdar ◽

Sehrish Murtaza ◽

Ghulam Mustafa ◽

...

Keyword(s):

Liver Cancer ◽

Cell Line ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Scavenging Activity ◽

Citrus Paradisi ◽

Anticancer Activities ◽

Artemisia Absinthium ◽

Cancerous Cell ◽

Dpph Radical Scavenging

Abstract Numerous reports show that herbal medicines can be utilized in the treatment of different liver disorders. In this study, antioxidant, antibacterial, and anticancer activities of individual as well as combined ethanolic extracts of Artemisia absinthium leaves and Citrus paradisi peels were investigated. Values of total phenolic contents (TPC), total flavonoid contents (TFC), DPPH-radical scavenging activity, and ferric reducing antioxidant power (FRAP) were measured to explore the antioxidant capacity. To assess antibacterial activity, four bacterial strains were used. For evaluation of anticancer activity Huh-7 (liver cancer) and Vero (non-cancerous) cell lines were utilized. FRAP activity of combined plants extract was higher as compared to their individual effect; the trend did not hold in case of DPPH-radical scavenging activity. Disk diffusion method revealed that only combined extract of plants express antibacterial activity against one strain (E. coli). MTT results indicated that both plants have cytotoxic effect on Huh-7 cell line and did not show any effect on Vero cell line. Our data showed a strong negative correlation between the amount of TPC, TFC, & DPPH radicals-scavenging activity and cell viability of cancerous cell line. Whereas, no effect was shown on non-cancerous cell line. Hence, these plants can be used in treatment of liver cancer because of their antioxidant, antibacterial, and anticancer activities.

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ANTI-PROLIFERATIVE ACTIVITY OF ZIZYPHUS SPINA CHRISTI LEAVES METHANOL EXTRACT AGAINST RHABDOMYOSARCOMA (RD) CELL LINE

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15013 ◽

2017 ◽

Vol 9 (2) ◽

pp. 279 ◽

Cited By ~ 1

Author(s):

Ahmed R. Abu-raghif ◽

Ghaith A. Jasim ◽

Muneer M. Hanoon

Keyword(s):

Antioxidant Activity ◽

Cell Line ◽

Proliferative Activity ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Cancer Cell Line ◽

Extraction Yield ◽

Gas Chromatography Mass Spectrometry ◽

Scavenging Activity

Objective: The aim of this study was to investigate the capability of Zizyphus spina christi methanol extract to inhibit cancer cell line proliferation.Methods: The leaves of Zizyphus spina christi were extracted by cold maceration method. The anti-proliferative activity of the methanol extract against rhabdomyosarcoma (RD) cell line was tested by 3-(4, 5 Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The phytochemical constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The antioxidant activity was assessed by measuring free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Results: The percentage extraction yield for leaves with methanol was 20.64%. The methanol extract showed dose dependent inhibition of RD cell line, the IC50 was 154.44 µg/ml. GC-MS showed the presence of flavonoid fraction and other compounds with antioxidant activity. The methanol extract demonstrated DPPH scavenging activity with IC50 of 33.91 mg/ml.Conclusion: Methanol extract showed potential anti-proliferative activity against RD cell line, which could be due to its antioxidant activity.

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In vitro Assessment the Potential Antioxidant and Antitumor Activities of Bifidobacterium Derived Bacteriocins

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.9.2.15 ◽

2019 ◽

Vol 9 (02) ◽

Author(s):

Shadan A Alwendawi

Keyword(s):

Antioxidant Activity ◽

Human Cancer ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Cancer Drug ◽

Scavenging Activity ◽

Antitumor Activities ◽

Human Cancer Cell Lines ◽

Mcf 7

Nowadays there are increasing interest in using microbial bioactive peptides as therapeutic agents or as adjuvant to increase the effectiveness of available therapies, a promising approach in this line is using of probiotics secreted peptides. Bifidobacterium is one of the favorite patented probiotics genera and most of human enteric Bifidobacterium secretes bacteriocins peptides to the surroundings. Bifidobacterial – associated bacteriocins are classified as GRAS peptides, and recently were attracted attention that become a widespread research topic in various fields including cancer drug discovery development. This study was conducted to seek for antioxidant and antitumor activities of bifidobacterial – derived bacteriocins. Two bacteriocins, Bifidin B1 and Bifidin B2, were partially purified from enteric Bifidibacterium longum Bl and Bifidobacterium bifidum B2, respectively. Bifidins were physiochemically characterized in respective of thermal, pH, and storage stability. Their proteinaceous nature was confirmed. Potential antioxidant activity in terms of free radical scavenging activity was evaluated, both Bifidins exhibited antioxidant activity, the highest percentage scavenging activity against DPPH was 70.55±0.2673 %, recorded for Bifidin B1, and was followed by 68.1 ±1.753% scavenging capacity for Bifidin B2, while both of Bifidins B1 and B2 had almost close values for scavenging of superoxide anion radicals, 66 ± 1.970 % and 65.64 ± 1.343%, respectively. Bifidins demonstrated potential antitumor activity on two human cancer cell lines, MCF-7 and Skov-3, however, the antiproliferative activities does not exceed 60.8%. Bifidins B1 and B2 showed highest cytotoxicity against Skov-3 cells rather than MCF-7 cells, with IC50 values of 28.9± 8.76 µg/ml, and 29.87± 9.13 µg/ml, respectively.

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