Analysis of the flupenthixol distribution in the internal organs of laboratory animals for the diagnosis of acute poisoning

2021 ◽  
Vol 64 (4) ◽  
pp. 60
Author(s):  
D.Yu. Sanzhieva ◽  
I.P. Remezova
Keyword(s):  
Acute Poisoning ◽  
Laboratory Animals ◽  
Internal Organs

MICROSCOPIC CHANGES IN THE INTERNAL ORGANS OF WHITE MICE DURING EXPERIMENTAL IRON (IV) CLATHROCHELATE TOXICOSIS

2021 ◽  
Vol 12 (4) ◽  
Author(s):  
B. V. Borysevych ◽  
◽  
V. V. Lisova ◽  
I. M. Derkach ◽  
S. S. Derkach ◽  
...  
Keyword(s):  
Biological Response ◽  
Organ Damage ◽  
The Body ◽  
New Drugs ◽  
Laboratory Animals ◽  
Internal Organs ◽  
Experimental Drug ◽  
Liver And Kidney ◽  
Preclinical And Clinical Studies ◽  
First Time

Iron (IV) clathrochelate based on a macrobicyclic ligand of the hexahydrazide type is a unique compound that contains iron in a rare high valence IV. Preclinical and clinical studies of this complex, which were started for the first time in Ukraine, have an important theoretical and practical consequence as this complex can be recommended as an active substance in iron-containing drugs with antianemic action. In conducting preclinical studies of new drugs, pathomorphological studies are important because they are a necessary step in studying the biological response of animals to the action of test substances. It was found that some pathological changes develop in the body of white mice under conditions of experimental acute and chronic iron (IV) clathrochelate intoxication. They correlated with the dose of the test compound. During chronic intoxication, the microscopic changes in the liver and kidney of white mice treated with iron (IV) clathrochelate at a dose of 1/10 DL50 were similar to the microscopic changes in the liver and kidney of mice treated with the experimental drug at a dose of 1/5 DL50. However, the severity of these changes was lower, reflecting a lower degree of organ damage. In the myocardium of mice treated with iron (IV) clathrochelate at a dose of 1/5 DL50 on the 10th day, as in acute iron (IV) clathrochelate poisoning, only edema was recorded. The prospects for further research are the study of microscopic changes in the organs of laboratory animals of other species during experimental iron (IV) clathrochelate toxicosis.

scholarly journals Distribution and Resorption of Intravenously Administrated Polymer Microparticles in Tissues of Internal Organs of Laboratory Animals

2009 ◽  
Vol 2 (4) ◽  
pp. 453-465 ◽  
Author(s):  
Shishatskaya Ekaterina I. ◽  
◽  
Goreva Anastasya V. ◽  
Kalacheva Galina S. ◽  
Volova Tatiana G. ◽  
...  
Keyword(s):  
Laboratory Animals ◽  
Internal Organs ◽  
Polymer Microparticles

scholarly journals Morphological Changes in the Internal Organs of Laboratory Animals under a Single Administration of Fe Nanoparticles

2011 ◽  
Vol 11 (2) ◽  
pp. 62-66
Author(s):  
Nikita Aleksandrovich Navolokin ◽  
◽  
Olga Viktorovna Matveeva ◽  
Galina Nikiforovna Maslyakova ◽  
Alla Borisovna Bucharskaya ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Laboratory Animals ◽  
Single Administration ◽  
Internal Organs ◽  
Fe Nanoparticles

scholarly journals Synthesis and Properties of Zinc-Modified Hydroxyapatite

2020 ◽  
Vol 11 (1) ◽  
pp. 10
Author(s):  
Daria Lytkina ◽  
Anastasiya Gutsalova ◽  
Dmitriy Fedorishin ◽  
Natalya Korotchenko ◽  
Rafik Akhmedzhanov ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Functional Materials ◽  
Nitrogen Adsorption ◽  
Laboratory Animals ◽  
Zinc Ions ◽  
Internal Organs ◽  
Surgical Implants ◽  
Entire Experiment ◽  
Inorganic Components ◽  
Xrd Method

Hydroxyapatites modified with metal ions are the main inorganic components of bone tissue and are approved for use as components for biocomposites and coatings for surgical implants. This study examined prototypes of functional materials for bone implants based on hydroxyapatite modified with zinc ions. Zinc-modified hydroxyapatite was composed and synthesized. Using the XRD method, the phase composition was established. Using SEM, EPMA, and low-temperature nitrogen adsorption (BET) methods, surface properties were investigated. Antibacterial activity and biocompatibility have been established. The studied materials have antimicrobial activity; the samples did not cause significant changes in either the internal organs or the general condition of laboratory animals during the entire experiment.

scholarly journals DEVELOPMENT OF AN INFLAMMATORY RESPONSE IN LABORATORY ANIMALS AT EXPERIMENTAL MYCOTIC INFECTION AND IMMOBILIZATION STRESS

2020 ◽  
pp. 65-71
Author(s):  
L. I. Drozdova ◽  
L. I. Timina ◽  
N. M. Semenikhina
Keyword(s):  
Inflammatory Response ◽  
Peripheral Blood ◽  
Stress Factors ◽  
Laboratory Animals ◽  
Laboratory Mice ◽  
Internal Organs ◽  
Simultaneous Exposure ◽  
Anatomical Changes ◽  
Second Stage ◽  
Unpleasant Odor

The data on the methods of modeling polyetiological stress in laboratory mice and its effect on the development of the inflammatory response and the nature of pathological and anatomical changes are presented. At the first stage, two groups of animals simulated polyetiological stress. For this, the animals were infected with micromycete intraperitoneally and left stationary for several hours a day. After that, infection was assessed by detecting micromycete in the peripheral blood of animals. At the second stage, the degree of occurrence and the nature of pathological and anatomical changes were studied with different methods of micromycete administration. It was noted that as a result of the simultaneous exposure to two stress factors, micromycetes in the peripheral blood were detected on the 8th day of the study. At the same time, the influence of one stress factor led to the generalization of the process only on the 17th day. With different methods of infection in laboratory mice, characteristic changes were revealed in the internal organs, which in all groups of animals were represented mainly as single and multiple abscesses of different localization, as well as fistulas in the left caudal part of the peritoneum. Abscesses in the region of the left forearm were filled with characteristic purulent contents: a curdled mass of light gray with an unpleasant odor or odorless. These pathological and anatomical changes were noted in 90% of the animals of their total number in the experiment. The introduction of R. syclopium followed by the exposure of white mice to IMO stress leads to 100% death of animals on days 1-2 from the onset of stress. Animals that were only injected with P. cyclopium remained alive until the end of the study.

scholarly journals ДОСЛІДЖЕННЯ ГОСТРОЇ ТА ХРОНІЧНОЇ ТОКСИЧНОСТІ ЕКСПЕРИМЕНТАЛЬНОГО ПРЕПАРАТУ «МЕВЕСЕЛ-ІН’ЄКЦІЙНИЙ»

2016 ◽  
Vol 6 (2) ◽  
pp. 174-180
Author(s):  
B. Gutyj ◽  
M. Paska ◽  
N. Levkivska ◽  
R. Pelenyo ◽  
N. Nazaruk ◽  
...  
Keyword(s):  
Blood Parameters ◽  
Laboratory Animals ◽  
Average Dose ◽  
Toxic Substances ◽  
Internal Organs ◽  
Accumulation Coefficient ◽  
Short Time ◽  
Increased Activity ◽  
Toxicity Criteria ◽  
Total Average

<p>The results of study of toxicity of the newly created «Injectable Mevesel» during acute and chronic experiments are presented. There were no lethal cases at intragastric and intramuscular injections, only short-time inhibition of laboratory animals receiving the drug at a dose of 10.0 ml was observed. There were no lethal cases of test animals during the experiment in the conditions of study of accumulation properties of «Injectable Mevesel». Total average dose of the drug administered made up 162500 mg/kg, and accumulation coefficient was respectively 5.3.<br />In the study of morphological blood parameters of rats after intramuscular injection of «Injectable Mevesel» in increasing doses, probable increase in neutrophils count by 36.1%, and probable reduction in lymphocytes count by 15.2% were found.<br />Administration of the drug in increasing doses significantly affects the functional state of internal organs of experimental animals (liver) and causes significant degradation of the membranes of hepatocytes, as evidenced by increased activity of intracellular ALT, AST enzymes and alkaline phosphatase.<br />Therefore, new domestic drug «Injectable Mevesel» created by us belongs to class 4 toxicity criteria, i.e. low-toxic substances.</p>

scholarly journals Visualization capabilities of experimental oncological models in small laboratory animals

2018 ◽  
Vol 9 (4) ◽  
pp. 105-112 ◽  
Author(s):  
Valeria A. Pechatnikova ◽  
Alexander P. Trashkov ◽  
Maria A. Zelenenko ◽  
Nikolay A. Verlov ◽  
Grigorii A. Chizh ◽  
...  
Keyword(s):  
Soft Tissues ◽  
Pathological Process ◽  
Nerve Fibers ◽  
Laboratory Animals ◽  
Small Laboratory ◽  
Internal Organs ◽  
Imaging Methods ◽  
Non Invasive ◽  
Long Time

For a long time non-invasive imaging methods have been inaccessible in preclinical practice; their introduction lately has broadened the boundaries of relevant studies and felicitated new approaches to solving fundamental problems. Up-to-date imaging methods constitute an essential component of preclinical and translational biomedical research allowing quick and non-invasive extended representation of structural organization and functional characteristics of pathological processes in vivo. Methods of radiation diagnosis and nuclear magnetic resonance allow to assess the state of bones, soft tissues, internal organs, blood vessels and peripheral nerve fibers in various animals, not only mammals, but also fish, amphibians, reptiles and insects. Multiparametric studies can uniquely localize any anatomical structure or pathological process. However, not all existing techniques are applicable to various oncological models of small laboratory animals.

Pathomorphological changes in internal organs in acute poisoning with 4-hydroxybutanoic acid (according to the data of the Bureau of forensic medical expertise)

2021 ◽  
pp. 28-34
Author(s):  
A. G. Sinenchenko ◽  
A. N. Lodyagin ◽  
T. V. Gorbacheva ◽  
G. I. Sinenchenko ◽  
B. V. Batotsyrenov
Keyword(s):  
Sudden Death ◽  
Lethal Dose ◽  
Biological Tissues ◽  
Acute Poisoning ◽  
Direct Connection ◽  
Internal Organs ◽  
Fatal Poisoning ◽  
Medical Expertise ◽  
Arterial Blood ◽  
Hydroxybutanoic Acid

The aim of the study was to establish objective reliable morphological signs of fatal poisoning with 4-hydroxybutanoic acid (4-HBA).Material and methods. The 49 acts of forensic medical examination of corpses with «Acute poisoning with 4-hydroxybutanoic acid» as the main cause of death were analyzed. The work was done in the Saint Petersburg Bureau of Forensic Medical Expertise.The results of morphological (macro - and microscopic) as well as forensic chemical studies of biological tissues were evaluated.The results were statistically processed using Statistic for Windows software (version 10). The numerical characteristics of the variables (M ± SD, Me [Q25; Q75]), χ2 — Pearson's criterion, and Spearman's correlation coefficient were applied for statistical processing.Results. The average lethal dose of 4-HBA in arterial blood was found to be equal to 273,6 ± 125,1 mg/l. In a forensic chemical study the combined use of 4-HBA and other narcotic substances (NS) and psychoactive substances (PAS) was diagnosed in 48.9% of cases. Of the causes leading to death, non-violent and «non-criminal» death due to 4-HBA poisoning was established more often - in 67.3% (33) of cases, a fall of the victim from a height with a fatal outcome - in 22.4% (11), less often violent death as a result of blunt and acute trauma - in 4.09% (2) cases.Among the macroscopic signs of acute lethal poisoning with 4-HBA were an increased mass of internal organs (heart, lungs, spleen, liver) in direct connection with the concentration of the toxicant in the blood. Microscopic signs of acute lethal poisoning by a toxicant included blood circulation features characteristic of sudden death.Concomitant pathology was diagnosed in 32.6% of cases in the form of degenerative changes in internal organs. In turn, hepatitis C and human immunodeficiency virus (HIV) were detected in 26.5% (13) of the dead.Conclusions. Thus, the signs characteristic for acute fatal poisoning with 4-HBA include the features of hemocirculation characteristic of sudden death. The brain is most severely affected, which is manifested by destructive edema. Poisoning is also characterized by the presence of serous pulmonary edema. Macroscopic signs of poisoning were revealed, including an increase in the weight of the heart, lungs, spleen and liver in direct connection with the concentration of the toxicant in the blood.It should be noted that the concomitant pathology in the form of dystrophic changes in the internal organs indicates the long-term nature of the use of the toxicant leading to pronounced metabolic disorders.

The influence of the feeding Flour Beetle Tribolium confusum-infested fodder on the selected indices of the health status of rats

2015 ◽  
Vol 18 (3) ◽  
pp. 499-506 ◽  
Author(s):  
Ł.B. Lis ◽  
T. Bakuła
Keyword(s):  
Health Status ◽  
Maximum Level ◽  
Tribolium Confusum ◽  
Female Rats ◽  
Farm Animals ◽  
Laboratory Animals ◽  
Control Group ◽  
Internal Organs ◽  
Beetle Population ◽  
The Impact

Abstract The present experiment was designed to demonstrate differences in the degree of fodder contamination with benzoquinones at various Tribolium confusum levels, the impact of infested feed on the beetle population and the impact of infested feed on the health status of rats. The feeding studies were done on female rats divided into 3 groups: a control group and two experimental groups. Experimental groups were fed with a fodder infested by 150 individuals of T. confusum per kg (group D1) and 300 individuals of T. confusum per kg (group D2). The insects were grown in the fodder for 5 months and the contaminated fodder was given to the laboratory animals for 8 weeks. After that period the rats were sacrificed, blood was drawn for morphological, biochemical and immunological analyses, as well as the samples of internal organs were taken for histopathology. Regardless of initial degree of infestation, after 5 months incubation period the content of benzoquinones in fodder reached the maximum level that reduced beetle population. The resulting concentration to benzoquinones had no effect upon feed intake nor growth of rate, whereas caused the presence of these substances in feces, urine and also in tissues which was indicated by pathological lesions observed in the study. The results obtained point to the possibility of the benzoquinones accumulation in the organisms of farm animals fed fodder containing pests.

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