scholarly journals The antibacterial role of photodynamic activation of nanoencapsulated photosensitizing agents in root canals.

2021 ◽  
Vol 10 (2) ◽  
pp. 1-3
Author(s):  
Pablo Betancourt ◽  
◽  
Miguel Viñas ◽  
Keyword(s):  
Root Canals ◽  
Photosensitizing Agents

The role of intracanal medicaments in inhibition of bacteria isolated from root canals of infected primary molars

2019 ◽  
Vol 14 (1) ◽  
pp. 92
Author(s):  
Dr. Maha Abdul- Kareem Mahmood ◽  
Dr. Huda Elias Ali ◽  
Dr. Haraa Khairi Abdul-Kadher
Keyword(s):  
Antimicrobial Activity ◽  
Streptococcus Mutans ◽  
Normal Saline ◽  
Root Canal ◽  
Primary Teeth ◽  
Primary Molars ◽  
Root Canals ◽  
Etiologic Agents ◽  
Micro Organisms

Microbes are considered as the primary etiologic agents in endodontic diseases.Disinfection of the root canal is obtained by the combined effect of biomechanicalpreparation, irrigation and intra canal medicament. The aim of the present study wasto assess the antimicrobial activity of intracanal medicaments (formocresol andEndosepton) against two micro organisms (Streptococcus mutans and staphylococcusaureus) isolated from 15 necrotic pulps of primary molars indicated for pulpectomyprocedure. The samples were cultured, and purified using microbiological evaluation.Broth dilution test was performed in our study by preparing test tubes containing10 ml of BHI broth (pH. 7) which then inoculated with strains of the tested bacteriaand incubated at 37 C° for 24 h. After over night incubaction, ten fold dilution weremade in test tubes containing 9 ml of normal saline by adding 1 ml of the inoculum tothe first tube . Then from dilution 10-1 , 0.1 ml of cell suspension was added to 9.9 mlof formocresol and endosepton, then 0.1 ml was taken and spread on duplicates ofBHI agar plates at different intervals and incubated aerobically for 24 h. at 37 C°.Colonies on the plates were counted after incubation and CFU/mL (colony formingunit) was calculated. Our results indicating that there were no significant differencesbetween the intracanal medicaments, but there were high significant differencesbetween the intervals time of the study. We concluded that both materials had greatantibacterial effect against the pathogens commonly isolated from necrotic pulpaltissue of primary teeth.

The role of mechanical instrumentation in the cleaning of root canals

2013 ◽  
Vol 29 (1) ◽  
pp. 87-109 ◽  
Author(s):  
Zvi Metzger ◽  
Michael Solomonov ◽  
Anda Kfir
Keyword(s):  
Root Canals

Role of Polymorphonuclear Neutrophils in the Clearance of Enterococcus faecalis Derived from Saliva and Infected Root Canals

2011 ◽  
Vol 37 (3) ◽  
pp. 346-352 ◽  
Author(s):  
Zeyun Ma ◽  
Yixiang Wang ◽  
Xiaofei Zhu ◽  
Chengfei Zhang ◽  
Shenglin Li ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Polymorphonuclear Neutrophils ◽  
Root Canals ◽  
Infected Root

scholarly journals New Light on the Brain: The Role of Photosensitizing Agents and Laser Light in the Management of Invasive Intracranial Tumors

2003 ◽  
Vol 2 (4) ◽  
pp. 303-309 ◽  
Author(s):  
M. Sam Eljamel
Keyword(s):  
Brain Tissue ◽  
Malignant Gliomas ◽  
Surgical Excision ◽  
Normal Brain ◽  
Full Potential ◽  
Intracranial Tumors ◽  
Dismal Prognosis ◽  
Photosensitizing Agents ◽  
The Brain

Invasive intracranial tumors, particularly malignant gliomas, are very difficult to eradicate surgically and carry a dismal prognosis. The vast majority relapse locally indicating that their cure is dependent on radical and complete local excision. However, their ability to invade and hide among normal brain tissue, our inability to visualize and detect them, the low tolerance of brain tissue to ionizing radiation and the presence of the blood brain barrier are the main causes of our failure to eradicate them. Photodynamic detection with 100% specificity and more than 80% sensitivity offers an excellent chance of visualizing camouflaged tumor nests. Also, photodynamic therapy offers a very good chance of targeted destruction of the remaining tumor cells safely following surgical excision and may double the survival of patients harboring these awful tumors. More work needs to be done to refine this promising technology to exploit it to its full potential.

scholarly journals Importance of bacterial endotoxin (LPS) in endodontics

2004 ◽  
Vol 12 (2) ◽  
pp. 93-98 ◽  
Author(s):  
Mario Roberto Leonardo ◽  
Raquel Assed Bezerra da Silva ◽  
Sada Assed ◽  
Paulo Nelson-Filho
Keyword(s):  
Biological Activity ◽  
Biological Effects ◽  
Periapical Lesion ◽  
Gram Negative ◽  
Periapical Lesions ◽  
Root Canals ◽  
Anaerobic Microorganisms ◽  
Technological Advances ◽  
Mineralized Tissues

New knowledge of the structure and biological activity of endotoxins (LPS) has revolutionized concepts concerning their mechanisms of action and forms of inactivation. Since the 1980's, technological advances in microbiological culture and identification have shown that anaerobic microorganisms, especially Gram-negative, predominate in root canals of teeth with pulp necrosis and radiographically visible chronic periapical lesions. Gram-negative bacteria not only have different factors of virulence and generate sub-products that are toxic to apical and periapical tissues, as also contain endotoxin (LPS) on their cell wall. This is especially important because endotoxin is released during multiplication or bacterial death, causing a series of biological effects that lead to an inflammatory reaction and resorption of mineralized tissues. Thus, due to the role of endotoxin in the pathogenesis of periapical lesions, we reviewed the literature concerning the biological activity of endotoxin and the relevance of its inactivation during treatment of teeth with pulp necrosis and chronic periapical lesion.

scholarly journals In Vivo Study of Calcium Hydroxide Endodontic Treatment in Chronic Apical Periodontitis

2018 ◽  
Vol 24 (4) ◽  
pp. 164-167
Author(s):  
Gheorghiu Irina-Maria ◽  
Mitran Loredana ◽  
M. Mitran ◽  
Temelcea Anca-Nicoleta ◽  
Scarlatesc Sanziana ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
Essential Role ◽  
Apical Periodontitis ◽  
Endodontic Treatment ◽  
Microbial Load ◽  
Root Canals

Abstract The aim of this study was to evaluate the in vivo efficiency of endodontic treatment with calcium hydroxide in chronic apical periodontitis by determining the microbial load from the endodontic space in different stages of treatment. The microbiological determinations that have been made have led to the conclusion that calcium hydroxide possesses a redoubltable antimicrobial activity, but only with the condition of long-lasting contact with the root canals. Another important result is the essential role of the rigorous biomechanical treatment of the root canals, intracanal medication with calcium hydroxide is addresses mostly to microorganisms located in root canal ramifications.

scholarly journals Evaluation of the Effect of MTAD on Expression of Enterococcus faecalis Virulence Factors Considering the Role of Different Obturating Materials

2019 ◽  
Author(s):  
Behnam Bolhari ◽  
Abbas Bahador ◽  
Mehrfam Khoshkhounejad ◽  
Mahsa Sobhi Afshar ◽  
Mohammad Moghaddaszadeh
Keyword(s):  
Enterococcus Faecalis ◽  
Virulence Factors ◽  
Root Canal ◽  
Statistical Power ◽  
P Value ◽  
Human Teeth ◽  
Root Canals ◽  
Gutta Percha ◽  
Post Hoc

Objectives: The aim of this study was to determine the effect of MTAD on the expression of virulence factors of Enterococcus faecalis (E.faecalis) considering the role of Gutta-percha/AH26 or Resilon/RealSeal SE as root canal obturating materials. Materials and Methods: One-hundred and forty-four single-rooted human teeth were instrumented to a standardized apical size. Root canals were infected by E.faecalis (ATCC 29212). Ninety teeth were irrigated with MTAD and randomly divided into three groups. In two groups, root canals were obturated by either Gutta-percha/AH26 or Resilon/RealSeal SE. Root canals were kept unobturated in the third group. The remaining 54 teeth received no final irrigation. All groups were then subdivided into three timepoint subgroups in which dentin powder was obtained from each sample to determine the expression of specific virulence factors of E.faecalis (efa, esp, gel, fsr) using real-time reverse transcription polymerase chain reaction (RT-PCR). Statistical analysis was performed by one-way analysis of variance (ANOVA) and Tukey’s post-hoc test. The statistical power was set at P-value ≤0.05. Results: MTAD was effective against the expression of most of the tested virulence factors, and Gutta-percha/AH26 increased the antibacterial efficacy of MTAD. Conclusions: MTAD could inhibit the expression of some known virulence factors of E.faecalis at the majority of tested timepoints. This may partly explain some of the mechanisms of antimicrobial efficacy of MTAD against this resistant microorganism which is known as one of the main causes of failure of root canal treatment.

scholarly journals Systematic Review on the Role of Lasers in Endodontic Therapy: Valuable Adjunct Treatment?

2020 ◽  
Vol 8 (3) ◽  
pp. 63
Author(s):  
Eugenia Anagnostaki ◽  
Valina Mylona ◽  
Steven Parker ◽  
Edward Lynch ◽  
Martin Grootveld
Keyword(s):  
Systematic Review ◽  
Pain Modulation ◽  
Treatment Modalities ◽  
Adjunct Treatment ◽  
Root Canals ◽  
Valuable Adjunct ◽  
Direct Irradiation ◽  
Root Canal System ◽  
Almost All

(1) Background: Adjunctive instruments, such as lasers have been investigated to address the risk of failure of endodontic therapy due to the complexity of the root canal system. Lasers have been used therapeutically, in direct irradiation of the root canals or adjunct to irrigants placed into the canals, in combination with a photosensitizer (antimicrobial photodynamic therapy) and in pain management (photobiomodulation). The purpose of this systematic review was to evaluate the evidence in clinical use within these three areas of therapy. (2) Methods: PubMed, Cochrane and Scopus search engines were used to identify human clinical trials regarding the use of lasers in endodontic therapy. (3) Results: After applying the keywords and additional filters, inclusion and exclusion criteria, the initial number of 1486 articles was reduced to 17. It was revealed that almost all studies (14/17) presented a statistically significant improved outcome in laser-assisted endodontic therapy, with the remaining three not showing any adverse effects. (4) Conclusions: The use of laser photonic energy of appropriate delivered parameters can be proposed as useful adjunctive when considering optimal treatment modalities in orthograde endodontics. Additionally, a tendency of research towards pain modulation in this field is developing.

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