scholarly journals The effect of induced training on selected equine blood plasma indicators on treadmill trained horses

2020 ◽  
Vol 65 (No. 12) ◽  
pp. 528-536
Author(s):  
M Massanyi ◽  
M Halo Jr. ◽  
A Kovacik ◽  
M Halo ◽  
I Imrich ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Creatinine Kinase ◽  
Total Proteins ◽  
Entire Duration ◽  
Mineral Profile ◽  
Visible Effect ◽  
Muscular Damage

The aim of this study was to evaluate the effect of induced training on the horses’ metabolism during an experiment lasting nine weeks where we continually scaled up the load on the horses by three defined stages. Blood was obtained from eighteen horses – two stallions, eight mares and eight geldings. In the experiment, we focused on the biochemical analysis of the blood plasma on multiple mineral profile indicators – Ca, P, Mg, K, Cl and Na, and some other variables (energy, nitrogen, AST, ALT, glucose, urea, creatinine kinase, total proteins). The result showed significant changes between the groups in most indicators. A significant increase in the potassium, phosphorus and calcium and a decrease in the concentrations of magnesium over the course of the experiment were found. For the other indicators, a significant increase in the activities of the AST and ALT out of the other indicators and the fluctuating values in the total proteins were noticed. Summarised, significant changes of multiple indicators were observed in different stages of the experiment. These changes had no visible effect on the horses’ organisms throughout entire duration of the experiment and were most probably caused by the muscular work and possible muscular damage during training.

scholarly journals Protein, energetic, enzymatic and mineral profile of Nellore cows during the pregnancy, parturition and postpartum

2020 ◽  
Vol 42 ◽  
pp. e49022
Author(s):  
Wagner Dias Coelho de Oliveira ◽  
Tairon Pannunzio Dias e Silva ◽  
Leílson Rocha Bezerra ◽  
Marcos Jácome de Araújo ◽  
Ricardo Loiola Edvan ◽  
...  
Keyword(s):  
Live Weight ◽  
Late Pregnancy ◽  
Experimental Period ◽  
Negative Energy ◽  
Normal Variation ◽  
Blood Biomarkers ◽  
Total Proteins ◽  
Mineral Profile ◽  
Calcium Phosphorus ◽  
Beta Hydroxybutyrate

The aim of this study was to evaluate the protein energetic, enzymatic and mineral profile of Nellore cows during the pregnancy, parturition and postpartum. Fifteen multiparous cows with 4 ± 1 years of age and live weight of 400 ± 50 kg were used at different stages (non-pregnant, in the initial, middle and late pregnancy, at birth, one day postpartum, 30 and 60 days postpartum). Blood collections were performed every 30 days and assayed for the following blood biomarkers: Protein (total proteins, albumin, urea and creatinine), energetic (cholesterol, triglycerides, glucose and beta hydroxybutyrate), mineral (calcium, phosphorus, magnesium) and enzymatic (alkaline phosphatase and aspartate aminotransferase). Calcium had the lowest concentrations (p < 0.05) in the initial pregnancy, while phosphorus had the highest concentration at parturition (p < 0.05). Triglycerides, glucose and beta hydroxybutyrate were influenced by the stages of pregnancy, reducing in the late pregnancy and at parturition. Glucose had a reduction in the late pregnancy and elevation in the postpartum. Beta hydroxybutyrate showed increase at the late pregnancy. Although lipomobilization occurred in the phases of higher metabolic demands in the attempt to maintain homeostatic conditions. Nellore cows did not present negative energy balance in the late pregnancy period, maintaining normal variation in blood markers throughout the experimental period.

scholarly journals Mineral Profile of Blood Plasma of Lactating Dairy Cows

1982 ◽  
Vol 65 (7) ◽  
pp. 1219-1226 ◽  
Author(s):  
P.A. McAdam ◽  
G.D. O’Dell
Keyword(s):  
Blood Plasma ◽  
Dairy Cows ◽  
Lactating Dairy Cows ◽  
Mineral Profile

scholarly journals Zinc as a feed supplement and its impact on plasma cholesterol concentrations in breeding cocks

2011 ◽  
Vol 80 (3) ◽  
pp. 281-285 ◽  
Author(s):  
Tomáš Parák ◽  
Eva Straková
Keyword(s):  
Blood Plasma ◽  
Total Cholesterol ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Plasma Cholesterol ◽  
Control Group ◽  
Total Proteins ◽  
Zinc Compounds ◽  
The Impact ◽  
In Blood Plasma

The aim of this work was to verify the impact of feed supplemented with selected inorganic and organic zinc compounds on the total cholesterol concentrations and other blood plasma indices in breeding cocks. A total of 250 RIR 05 breeding cocks, 9 weeks old, were used. The cocks were divided into 5 groups of 50 animals each (four experimental groups and one control group). Cocks were fed a commercial feed mixture specifically for breeding cocks, containing 30.4 mg·kg-1 of zinc. The feed for experimental groups of cocks was fortified to 100 mg Zn·kg-1: zinc sulphate in first group, zinc oxide in second group, fodder yeast (Minvital Zn) in third group and Bioplex Zn in fourth group. Blood samples for biochemical examination were taken from the basilic vein. The contents of total cholesterol (Chol), total proteins (TP), glucose (Glu), aspartate aminotransferase (AST), alanine aminotransferase (ALT), calcium (Ca), phosphorus (P) and magnesium (Mg) in blood plasma were spectrophotometrically measured using a biochemical analyser, Cobas EMira, and commercial kits (Biovendor a.s., Czech Republic). In the 15th week of age, third and fourth groups showed a significant (P ≤ 0.05) and highly significant (P ≤ 0.01) decrease of total cholesterol in blood plasma compared to the control. In the 20th and 25th week of age, all of the experimental groups showed a significantand highly significant (P ≤ 0.01) decrease of total cholesterol in blood plasma compared to the control. Other monitored indices (total proteins, glucoses, aspartate aminotransferase, alanine aminotransferase, calcium, phosphorus and magnesium) did not reveal any significant changes between the experimental and the control groups. The presented work provides the first available experimental evidence regarding the impact of zinc supplementation on the cholesterol levels in blood plasma of breeding cocks.

scholarly journals Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods

2005 ◽  
Vol 48 (3) ◽  
pp. 385-388 ◽  
Author(s):  
Dimas Augusto Morozin Zaia ◽  
Fábio Rangel Marques ◽  
Cássia Thaïs Bussamra Vieira Zaia
Keyword(s):  
Comparative Study ◽  
Blood Plasma ◽  
Total Protein ◽  
Total Proteins ◽  
Erythrosin B ◽  
Spectrophotometric Methods ◽  
Biuret Method ◽  
Bradford Method ◽  
In Blood Plasma

A comparative study between the biuret method (standard method for total proteins) and spectrophotometric methods using dyes (Bradford, 3',3",5',5"-tetrabromophenolphthalein ethyl ester-TBPEE, and erythrosin-B) was carried out for the determination of total proteins in blood plasma from rats. Bradford method showed the highest sensitivity for proteins and biuret method showed the lowest. For all the methods, the absorbance for different proteins (BSA, casein, and egg albumin) was measured and Bradford method showed the lowest variation of absorbance. The concentration of total protein obtained by using Bradford method was not statistically different (p>0.05) from concentration of total protein obtained by the biuret method. But in regard to erythrosin-B and TBPEE methods the concentrations of total protein were statistically different (p<0.05). Thus, Bradford method could be used instead of the biuret method for determination of total proteins in blood plasma.

scholarly journals Blood Plasma Metabolic Profile of Aberdeen Angus Bulls during Postnatal Ontogenesis

2010 ◽  
Vol 79 (3) ◽  
pp. 419-429 ◽  
Author(s):  
Aleš Pavlík ◽  
Pavel Jelínek ◽  
Martin Matějíček ◽  
Josef Illek
Keyword(s):  
Blood Plasma ◽  
Postnatal Development ◽  
Metabolic Profile ◽  
Correlation Coefficients ◽  
Inorganic Phosphorus ◽  
Total Proteins ◽  
Significant Relationships ◽  
Activity Temperature ◽  
Copper Zinc ◽  
Relationship Of

Selected indicators for the metabolic profile of blood plasma in 22 Aberdeen Angus bulls reared by the extensive method were monitored during postnatal development (from 4 to 564 days of age), including total proteins, albumin, urea, creatinine, glucose, cholesterol, triacylglycerols, bilirubin, alkaline phosphatase, alanine transaminase, aspartate transaminase, tyroxin, triiodothyronine, calcium, inorganic phosphorus, magnesium, potassium, sodium, chlorides, copper, zinc and iron. In assessing the relationship of age to the indicators of the internal environment, values of correlation coefficients for total proteins (r = –0.70;p< 0.01), albumin (r = –0.56;p< 0.01), urea (r = –0.73;p< 0.01), glucose (r = –0.38;p< 0.01) and triiodothyronine (r = –0.82;p< 0.01) were established. Significant relationships were shown between the temperature of the external environment and the amount of total proteins (r = –0.38;p< 0.01), urea concentration (r = –0.41;p< 0.01), ALP (r = 0.58;p< 0.01) and ALT (r = 0.45;p< 0.01) activity. Temperature also showed a significant impact on the concentration of P (r = 0.57;p< 0.01), K (r = –0.69;p< 0.01) and Zn (r = 0.33;p< 0.01). The work yields important information on changes in the indicators of the metabolic profile of the blood plasma of bulls during postnatal development under defined nutritional and temperature conditions that can be used as reference values for evaluating health status as well as nutrition level.

scholarly journals Blood Plasma Mineral Profile and Qualitative Indicators of the Eggshell in Laying Hens in Different Housing Systems

2009 ◽  
Vol 78 (3) ◽  
pp. 419-429 ◽  
Author(s):  
Aleš Pavlík ◽  
Martina Lichovníková ◽  
Pavel Jelínek
Keyword(s):  
Blood Plasma ◽  
Laying Hens ◽  
Breaking Strength ◽  
Housing System ◽  
Shell Quality ◽  
Housing Systems ◽  
Cage System ◽  
Mineral Profile ◽  
Egg Shell ◽  
Mineral Concentrations

The aim of this study was to compare the blood plasma mineral profile (Ca, P, K, Mg, Zn, Cu and Se) and egg-shell quality (eggshell weight, eggshell breaking strength and thickness) of laying hens housed in three different housing systems (traditional cage system, enriched cage system and deep litter system). In each housing system, 12 ISA Brown laying hens were observed during the laying period from week 22 to 75 of age. The effect (p < 0.05 and p < 0.01) of age and/or phase of the laying cycle on all mineral concentrations was determined. Eggshell breaking strength decreased (p < 0.001) with the age of birds. The results of this study indicate that the housing systems compared had no significant effect on the blood plasma mineral profile of laying hens under study and the values were within the physiological range. However, a significant effect of housing system on eggshell breaking strength and eggshell weight was found. Improved eggshell quality was obtained in most periods of the laying cycle in the enriched cage systems.

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