scholarly journals Growth, photosynthesis and antioxidant defense systém in Zn-deficient red cabbage plants

2010 ◽  
Vol 56 (No. 5) ◽  
pp. 209-217 ◽  
Author(s):  
R. Hajiboland ◽  
F. Amirazad
Keyword(s):  
Superoxide Dismutase ◽  
Chlorophyll A ◽  
Antioxidant Defense ◽  
Soluble Carbohydrates ◽  
Zn Deficiency ◽  
Lower Sensitivity ◽  
Chlorophyll Fluorescence Parameters ◽  
Red Cabbage ◽  
Malondialdehyde Content ◽  
Reduction Of Co2

The effect of Zn deficiency was studied in red cabbage (Brassica oleracea L. var. capitata f. rubra) plants grown in nutrient solution under controlled environmental conditions. Zinc starvation affected the number (61%), surface area (72%) and biomass (62%) of leaves more than root biomass (42%). Although chlorophyll fluorescence parameters revealed occurrence of photoinhibition following declined stomatal conductance and reduction of CO2 available at carboxylation sites, photosynthesis apparatus was not damaged seriously under Zn deficiency conditions. Chlorophyll a, chlorophyll a/b ratio, soluble carbohydrates and starch declined but anthocyanins and free phenolics were accumulated under Zn deficiency conditions. Activity of ascorbate peroxidase, catalase and peroxidase enhanced under Zn deficiency conditions, whereas activity of superoxide dismutase declined in leaves but not in roots of Zn-deficient plants. Maintenance of superoxide dismutase activity and malondialdehyde content in roots demonstrated that roots were more protected against reactive oxygen species imbalance under Zn deficiency conditions compared with leaves that was correlated well with the lower sensitivity of roots to low Zn supply.

scholarly journals Photochemistry and gas exchange in cold conditions in Zn-deficient red cabbage (Brassica oleracea L. var. capitata f. rubra) plants

2012 ◽  
Vol 24 (1) ◽  
pp. 3-11
Author(s):  
Roghieh Hajiboland ◽  
Fahimeh Amirazad
Keyword(s):  
Gas Exchange ◽  
Brassica Oleracea ◽  
Low Temperatures ◽  
Soluble Sugars ◽  
Cold Treatment ◽  
Soluble Carbohydrates ◽  
Shoot Biomass ◽  
Zn Deficiency ◽  
Ps Ii ◽  
Red Cabbage

Photochemistry and gas exchange in cold conditions in Zn-deficient red cabbage (Brassica oleraceaL. var.capitataf.rubra) plantsThe responses of red cabbage (Brassica oleraceaL. var.capitataf.rubra) plants to a low Zn supply and cold conditions (10°/7°C day/night temperature) were investigated in a hydroponic growing medium. A low Zn supply caused a significant reduction of shoot and root dry weight - up to 55% and 45% for the control and 62% and 52% for cold-treated plants, respectively. The total soluble carbohydrates and starch declined in Zn-deficient plants. Exposure to low temperatures, however, led to a decline in starch but an increase in soluble sugars. In Zn-sufficient plants, low temperatures increased the excitation capture efficiency of open photosystem II (PS II) reaction centres (RCs) (F'v/F'm), the quantum yield of PS II (ΦPSII), the electron transport rate (ETR) and the proportion of active chlorophyll associated with the RCs of PS II (Fv/F0). Low temperatures did not affect net CO2uptake in Zn-sufficient plants, though a reduction of stomatal conductance occurred. The results demonstrated that although cold-treated plants were slightly more susceptible to Zn deficiency, cold treatment caused greater shoot biomass (up to 32%) in plants supplied with adequate Zn. The adaptation of red cabbage plants to cold conditions is attributable to improved photochemical events in the leaves, a maintenance of the net CO2assimilation rate, lower water loss and the accumulation of anthocyanins as antioxidants.

STUDY OF THE COMBINED INFLUENCE OF SODIUM THIOPENTAL AND DELTA SLEEP-INDUCING PEPTIDE ON ANTIOXIDANT DEFENSE SYSTEM IN RATS

2016 ◽  
pp. 49-52
Author(s):  
A. V. Shvetsov ◽  
E. G. Batotsyrenova ◽  
N. A. Dyuzhikova ◽  
V. A. Kashuro ◽  
N. V. Lapina ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Time Interval ◽  
Sodium Thiopental ◽  
Antioxidant Defense Enzymes ◽  
Delta Sleep ◽  
Thiopental Coma ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Delta Sleep Inducing Peptide

A biochemical investigation was performed into activity of rat antioxidant defense enzymes at different time interval after administration of sodium thiopental and delta-sleep-inducing peptide (DSIP). It was shown that thiopental coma was accompanied by a decreased level of superoxide dismutase ( 6 and 24 h after exposure) and increased level of caspase-3 ( 6 h after exposure) in the rat blood plasma. A pharmacological correction with DSIP induced a decrease of the level of superoxide dismutase ( 6 and 24 h after exposure), glutathione peroxidase and glucose 6-phosphate dehydrogenase (after 6h).

scholarly journals Phytochemical changes in mango fruit in response to Alternaria alternata infection

2018 ◽  
Vol 36 (No. 3) ◽  
pp. 227-232 ◽  
Author(s):  
Jiao Li ◽  
Liangang Mao ◽  
Yanning Zhang ◽  
Lan Zhang ◽  
Hongyun Jiang
Keyword(s):  
Superoxide Dismutase ◽  
Vitamin C ◽  
Fruit Quality ◽  
Alternaria Alternata ◽  
Enzyme Activities ◽  
Phenylalanine Ammonia Lyase ◽  
Titratable Acidity ◽  
Mango Fruit ◽  
Malondialdehyde Content

Changes in mango fruit quality, malondialdehyde content, and enzymatic activities in response to pathogen Alternaria alternata infection were studied. A. alternata significantly affected the appearance of mango fruit at 5 and 7 days after treatment (DAT). The quality of pathogen-infected fruit first showed a significant decrease in titratable acidity and vitamin C content and a significant increase in pH since 3 DAT. The malondialdehyde content was higher than that in the untreated controls at 3 and 7 DAT. The enzyme activities of ascorbate peroxidase and polyphenol oxidase showed significant increases since 3 DAT. Significant increases in l-phenylalanine ammonia-lyase and superoxide dismutase activities were observed at 7 DAT. These results indicate that A. alternata infection first significantly affects some biochemical constituents and enzyme activities in mango fruit since 3 DAT and that there was no significant effect on appearance until 5 DAT.

scholarly journals Antioxidant Enzyme Activities of some Brassica Species

2017 ◽  
Vol 74 (2) ◽  
pp. 191
Author(s):  
Rodica SOARE ◽  
Maria DINU ◽  
Cristina BĂBEANU ◽  
Mihaela POPESCU
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Enzymatic Activity ◽  
Brassica Oleracea ◽  
Radical Cation ◽  
Colorimetric Method ◽  
Antioxidant Enzyme Activities ◽  
Red Cabbage ◽  
Soluble Peroxidase ◽  
Brassica Oleracea L

This paper set out to comparatively study five species: white cabbage (Brassica oleracea L. var. capitata alba Alef.), red cabbage (Brassica oleracea L. var. capitata f. rubra Alef.), Kale (Brassica oleracea L. var. Acephala), cauliflower (Brassica oleracea var. botrytis) and broccoli (Brassica oleracea var. cymosa) in order to identify those with high enzymatic and antioxidant activities. The enzymatic activity of superoxide dismutase (SOD), catalase (CAT) and soluble peroxidase (POX) as well as the antioxidant activity against 2.2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation were determined. Total superoxide dismutase activity was measured spectrophotometrically based on inhibition in the photochemical reduction of nitroblue tetrazolium. Total soluble peroxidase was assayed by measuring the increase in A436 due to the guaiacol oxidation and the catalase activity was assayed through the colorimetric method. The capacity of extracts to scavenge the ABTS radical cation was assessed colorimetric using Trolox as a standard. The obtained results show that studied enzymatic activities and the antioxidant activity against ABTS vary depending on the analyzed species. So, among the studied Brassicaceae species, it emphasize red cabbage with the highest enzymatic activity (CAT 22.54 mM H2O2/min/g and POX 187.2 mM ΔA/1min/1g f.w.) and kale with highest antioxidant activity, of 767 μmol TE/100g f.w. The results of this study recommendintroducing the studied varieties in diet due to the rich antioxidant properties.

scholarly journals INFLUENCE OF «METISEVIT» ON THE ACTIVITY OF ENZYME AND NONENZYME LINK OF ANTIOXIDANT PROTECTION UNDER THE BULL’S BODY CADMIUM LOADING

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 52-59
Author(s):  
B.V. Gutyj ◽  
Y. Lavryshyn ◽  
V. Binkevych ◽  
O. Binkevych ◽  
О. Paladischuk ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Vitamin E ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Cadmium Stress ◽  
Antioxidant Defense System ◽  
The Body ◽  
Antioxidant Protection ◽  
Young Cattle

The article contains the research results of the effect of cadmium chloride on the indexes of enzyme and nonenzyme systems of  antioxidant defense system in young cattle, such as the activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels of vitamins A and E. It is established that feeding calves at a dose of toxicant 0.04 mg / kg activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels of vitamins A and E in the blood of experimental animals decreased throughout the experiment. The lowest indicators of antioxidant in the blood of young cattle is set on the twenty -fourth day of the experiment, which is associated with increased activation of lipid peroxidation and the balance between antioxidant system and lipid peroxidation intensity. Given the cadmium load of young cattle it is used a new integrated drug with antioxidant action «Metisevit», which includes metifen, sodium selenite and vitamin E wich is founded as stimulating effects on the activity of antioxidant protection. In particular,it is established probable increase in activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels, vitamin A and vitamin E in the blood of young cattle, which has performed cadmium stress. These changes occur through comprehensive action components of the drug «Metisevit» that leads to the normalization of metabolic processes and free radical in the body of the bull. The results of the research indicate antioxidant drug «Metisevit» in the application of its young cattle and the validity of his administration to improve the body's antioxidant status of chronic cadmium toxicosis.

scholarly journals Corrective effect of red wine concentrate enriched with natural complex of polyphenols on activity of antioxidant defense enzymes in cardiac muscle under experimental diabetes mellitus

2021 ◽  
Vol 15 (1) ◽  
pp. 37-48
Author(s):  
D. Yu. Hertsyk ◽  
◽  
M. V. Sabadashka ◽  
L. V. Kaprelyants ◽  
N. O. Sybirna ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Red Wine ◽  
Experimental Diabetes ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Experimental Diabetes Mellitus ◽  
Corrective Effect ◽  
Antioxidant Defense Enzymes

Background. Diabetes mellitus is accompanied by oxidative-nitrative stress, which is caused both by an increased generation of the Reactive Oxygen Species and the Reactive Nitrogen Species under the conditions of this pathology and by the disorders of the antioxidant defense system, especially its enzymatic part. This leads alterations in the morpho-functional state of cells, organs, and the whole organism. Free radicals are involved in the destruction of pancreatic cells in type 1 diabetes mellitus, which leads to even greater inhibition of insulin secretion, worsening of the course of the disease and occurrence of diabetic complications. The complications of diabetes mellitus include often diagnosed cardiovascular diseases. Therefore, the use of treatments characterized not only by hypoglycemic properties for normalization of blood sugar level in diabetes mellitus, but also by antioxidant properties for normalization of oxidative/antioxidant balance of the organism in the studied pathology can be promising. Scientists pay a great attention to the study of substances with such properties, especially natural phenolic compounds of grape, which are also characterized by immunomodulatory properties. Materials and Methods. The study was performed on cardiac tissues of control rats, control animals treated with wine polyphenolic complex concentrate, animals with streptozotocin-induced diabetes mellitus and animals with experimental diabetes mellitus treated with a concentrate of wine polyphenolic complex. The activity of catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase were detected to examine the corrective effect of the concentrate of red wine natural polyphenolic comp­lex on the state of the enzymatic part of the antioxidant defense system. Results. The results have shown the normalization of activity of catalase, superoxide dismutase, and changes in the activity of enzymes of glutathione cycle after oral administration of polyphenolic complex concentrate for 14 days to rats with streptozo­tocin-induced diabetes mellitus. Conclusions. The results confirm a hypothesis about the antioxidant effect of the studied concentrate and the ability of natural polyphenolic complexes to serve as the basis for new drugs for treatment of diabetes-induced disorders.

scholarly journals Physıologıcal response of Apocynum venetum L. seedlıngs under osmotıc stress

2018 ◽  
Vol 44 (4) ◽  
pp. 551-556
Author(s):  
Hua Zhao ◽  
Huiping Dai
Keyword(s):  
Superoxide Dismutase ◽  
Polyethylene Glycol ◽  
Drought Stress ◽  
Physiological Response ◽  
Antioxidant Defense ◽  
Soluble Sugar ◽  
Defense Systems ◽  
Apocynum Venetum ◽  
Antioxidant Defense Systems ◽  
Effects Of Drought

Effects of drought stress were induced by polyethylene glycol (PEG-6000) (10, 20, and 30% ) for 2, 4, 6 and 8 days in Apocynum venetum L. seedlings with the potting method. The results showed that PEG significantly increased the activities of glutathione reductase (GR), superoxide dismutase (SOD), ascorbate peroxidase (APX), and the contents of electrolyte leakage rates, MDA, proline and soluble sugar were increased from 30% PEG. Thus, it was indicated that the application of exogenous 30% PEG induced oxidative damage by enhancing antioxidant defense systems.

Effect of Oil Water-Accommodated Fraction on Growth and Chlorophyll-A of Marine Microalgae

2013 ◽  
Vol 726-731 ◽  
pp. 94-97
Author(s):  
Yu Bai Zhang ◽  
Xue Xi Tang
Keyword(s):  
Chlorophyll A ◽  
Isochrysis Galbana ◽  
Chlorophyll Fluorescence Parameters ◽  
Fluorescence Parameters ◽  
Nitzschia Closterium ◽  
Oil Water ◽  
Chlorophyll A Content ◽  
Platymonas Helgolandica ◽  
Toxicity Effects ◽  
Water Accommodated Fraction

The experiment studied the 96h acute toxicity effects on three kinds of microalgae caused by Water-Accommodated Fraction (WAF), and effects of different concentrations WAF (Platymonas helgolandicavar.tsingtaoensis,Nitzschia closteriumf.minutissima00.524816 mg/L;Isochrysis galbana00.52.551020 mg/L) on chlorophyll-a content and chlorophyll fluorescence parameters. It showed that the WAF 96h EC50of thePlatymonas helgolandicavar.tsingtaoensis,Nitzschia closteriumf.minutissima,Isochrysis galbanawere 13.84mg/L, 18.83mg/L and 6.73mg/L. The level of WAF sensitivity wasNitzschia closteriumf.minutissima>Platymonas helgolandicavar.tsingtaoensis>Isochrysis galbana. The WAF caused inhibition of chlorophyll-a content except in condition of the low concentration. It showed the higher of the WAF concentration, the stronger the inhibition.

scholarly journals Effect of Microelements and Selenium on Superoxide Dismutase Enzyme, Malondialdehyde Activity and Grain Yield Maize (Zea mays L.) under Water Deficit Stress

2011 ◽  
Vol 39 (2) ◽  
pp. 153 ◽  
Author(s):  
Nourali SAJEDI ◽  
Hamid MADANI ◽  
Ahmad NADERI
Keyword(s):  
Superoxide Dismutase ◽  
Water Stress ◽  
Grain Yield ◽  
Water Deficit ◽  
Block Design ◽  
Stress Conditions ◽  
Water Deficit Stress ◽  
Growth Stages ◽  
Malondialdehyde Content ◽  
Main Plot

This study was carried out to investigate effects of microelements under water deficit stress at different growth stages on antioxidant enzyme alteration, chemical biomarker and grain yield of maize in the years 2007 and 2008. The experiment was conducted in a split plot factorial based on a randomized complete block design with four replications. There were three factors, water deficit stress at different stages of growth as main plot and combinations of selenium (with and without using) and microelements (with and without using) as sub plots. The result indicated that the activity of superoxide dismutase and malondialdehyde content under water deficit stress increased, but grain yield was reduced. The highest grain yield was obtained from optimum irrigation, while in the case of with water deficit stress at V8 stage it was non significant. Selenium spray increased activity of superoxide dismutase enzyme, malondialdehyde content of leaves in V8, R2 and R4 stages and also grain yield. Application of microelements increased the leaves superoxide dismutase enzyme activity and malondialdehyde content. Selenium and microelements spray under water deficit stress conditions during vegetative growth and dough stage increased grain yield in comparison to not spraying elements under water stress conditions. The present results also showed that by using selenium and microelements under water stress can obtain acceptable yield compared to not using these elements.

scholarly journals Antitumor activity of tanshinone and its nanoparticles on U14 cervical carcinoma-bearing mice

2016 ◽  
Vol 3 ◽  
pp. 184954351667344
Author(s):  
Ya Di ◽  
Qingjie Meng ◽  
Hongwei Yang ◽  
Kun Li ◽  
Liyan Cao ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Cervical Carcinoma ◽  
Antitumor Effect ◽  
Salvia Miltiorrhiza ◽  
Control Group ◽  
High Dose ◽  
Tumor Inhibition ◽  
Malondialdehyde Content ◽  
Model Control ◽  
Model Control Group

In this study, tanshinone was extracted from Salvia miltiorrhiza. To improve the utilization and the dissolution of the drug, the tanshinone extractions were prepared at a pharmaceutical nanoscale and in the nanometer range of 100–200 nm. Then, the rate of tumor inhibition and the activity of antioxidant system and the thymus/spleen indices were investigated to find the antitumor effect of nanoparticles of tanshinone in cervical carcinoma-bearing mice. Our data suggest that tanshinone inhibits cervical tumor growth and the rates of tumor inhibition of all drug groups were more than 45%. The highest rate was 70.88% in the high dose of nanoscale tanshinone group. The activities of superoxide dismutase were higher in drug groups than in the model control group, and the concentrations of malondialdehyde were significantly lower. These findings suggested that tanshinone enhance the superoxide dismutase activity of the mice and decrease the malondialdehyde content. It may be one of the mechanisms of antitumor effect of tanshinone. The thymus index and spleen index were higher than normal control or model control. These data suggested that tanshinone also enhanced the immune system of mice.

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