scholarly journals Malondialdehyde levels in serum of dogs infected with Babesia canis

2010 ◽  
Vol 55 (No. 4) ◽  
pp. 163-171 ◽  
Author(s):  
M. Crnogaj ◽  
R. Petlevski ◽  
V. Mrljak ◽  
I. Kis ◽  
M. Torti ◽  
...  
Keyword(s):  
Statistical Difference ◽  
Nitrogen Concentration ◽  
Laboratory Data ◽  
Polymerase Chain Reaction Method ◽  
Babesia Canis ◽  
Mild Disease ◽  
Significant Statistical Difference ◽  
Blood Smears ◽  
Healthy Dogs ◽  
Polymerase Chain

Babesiosis is a common tick born disease of dogs in tropical and subtropical regions of the world caused by different species of Babesia. The aim of the present study was to confirm the presence of oxidative stress by examining serum malondialdehyde (MDA), an end product of lipid peroxidation, in 35 dogs naturally infected with Babesia canis (B. canis). MDA was examined in 14 healthy dogs as well. Blood smears were prepared from peripheral blood and they showed the presence of B. canis in infected dogs. B. canis was confirmed using the PCR (Polymerase chain reaction) method. On the basis of clinical and laboratory data the 35 infected dogs were clinically classified into two groups, complicated (seven dogs) and uncomplicated (28 dogs). The noted complications were renal dysfunction (5/7), hepatic dysfunction (3/7), muscular involvement (2/7) and ARDS (1/7). Levels of blood urea nitrogen concentration (BUN), creatinin, total bilirubin, alanin aminotranspherase (ALT), alkaline phosphatase (AP), and gammaglutamil aminotranspherase (GGT) were significantly increased in dogs with complicated versus uncomplicated babesiosis. Furthermore the uncomplicated group of dogs was, depending on the severity of anaemia, classified as suffering from severe, moderate or mild disease. Levels of serum MDA were significantly higher in sick dogs (36.90 μmol/l ± 13.95) than healthy animals (8.13 μmol/l ± 1.78). There was no significant statistical difference in serum MDA levels between dogs with complicated (38.48 μmol/l ± 12.11) and uncomplicated babesiosis (36.50 μmol/l ± 14.55). Comparison of the groups based on the severity of anaemia showed that there was no significant statistical difference in serum MDA levels between them. The study demonstrated the involvement of oxidative damage in dogs naturally infected with B. canis.

scholarly journals Antioxidant status, and blood zinc and copper concentrations in dogs with uncomplicated babesiosis due to Babesia canis infections

2021 ◽  
Vol 65 (2) ◽  
pp. 169-174
Author(s):  
Oliwier Teodorowski ◽  
Stanisław Winiarczyk ◽  
Duygu Tarhan ◽  
Banu Dokuzeylül ◽  
Alev Meltem Ercan ◽  
...  
Keyword(s):  
Babesia Canis ◽  
Antioxidant Defence ◽  
Blood Concentrations ◽  
Naturally Occurring ◽  
Blood Smears ◽  
Zinc Concentrations ◽  
Healthy Dogs ◽  
Peripheral Blood Smears ◽  
Blood Zinc ◽  
Apparently Healthy

Abstract Introduction The aim of the study was to demonstrate a link between uncomplicated Babesia canis infection in dogs and blood concentrations of zinc and copper and erythrocytic antioxidant defence – activities of glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT). Material and Methods The study was based on 15 naturally occurring cases of canine babesiosis with anorexia, pyrexia, depression, pale mucous membrane, splenomegaly and dark red urine. Microscopic examination of Giemsa-stained peripheral blood smears and the results of PCR confirmed B. canis infection. Seven apparently healthy dogs brought in for either a check-up or vaccination were used for comparison. Results The levels of the erythrocytic antioxidant enzymes - SOD and CAT - were significantly higher in the infected dogs than in cytologically negative dogs. The levels of blood micronutrients were significantly lower in the infected dogs (0.478 μg of zinc per mL vs 1.241 μg/mL and 0.722 μg of copper per mL vs 1.392 μg/mL). Conclusion Oxidative stress can be posited as one of the mechanisms leading to anaemia in dogs with babesiosis, and therefore antioxidant biomarker and copper and zinc concentrations could be used as indicators of disease severity and prognostic markers.

scholarly journals Echocardiographic evaluation of the left atrium of healthy dogs using the conventional M-mode and the bidimensional mode

2012 ◽  
Vol 64 (3) ◽  
pp. 585-592 ◽  
Author(s):  
D.G. Prada ◽  
V.M.C. Oliveira ◽  
M.H.M.A. Larsson ◽  
F.L. Yamaki
Keyword(s):  
Confidence Interval ◽  
Left Atrium ◽  
Body Surface ◽  
Statistical Difference ◽  
Healthy Adult ◽  
Short Axis ◽  
High Positive Correlation ◽  
Significant Statistical Difference ◽  
Echocardiographic Evaluation ◽  
Healthy Dogs

The left atrium (LA) was evaluated through the bidimensional method (2-D) (short axis) and the relation left atrium:aorta (LA:Ao) in a group of 40 healthy adult dogs between 1.5 and 7 years of age and weights ranging from 3.2 to 38.3kg, and these values were compared to those in the conventional M-mode. Comparing the variable LA and Ao with the two methods and their respective indexes, significant statistical difference was observed (P<0,001), high positive correlation between the weight and LA-2D and LAM, between body surface and LA-2D and LAM. A difference between LA and Ao in both methods and these respective indicators were observed. The association was higly positive between weight and body surface area (BSA) and between M- mode LA (LAM) and 2-D mode LA (LA-2D). No correlation was observed between these indicators and weight and BSA, in both methods, so these indicators are weight and BSA-independent. Conclusions: LA-2D is bigger than LAM, the LA-2D:Ao-2D average index is 1,379 and the confidence interval is 1,337 to 1,422. The index in the 2-D method is, therefore, superior to the index in the M-mode.

scholarly journals Postoperative analgesia effects of sulfentanyl plus dexmedetomidine in patients received VATS

Pteridines ◽  
2020 ◽  
Vol 31 (1) ◽  
pp. 55-60
Author(s):  
Haoyu Jiang ◽  
Ying Zheng ◽  
Chang Liu ◽  
Ying Bao
Keyword(s):  
Statistical Difference ◽  
Control Group ◽  
Control Groups ◽  
Significant Statistical Difference ◽  
Post Surgery ◽  
Intravenous Analgesia ◽  
The Mean ◽  
Nsclc Patients ◽  
And Control ◽  
Time Point

AbstractBackground To evaluate sulfentanyl combined with dexmedetomidine hydrochloride on postoperative analgesia in patients who received video-assisted thoracic surgery (VATS) and its effects on serum norepinephrine (NE), dopamine (DA), 5-hydroxytryptamine (5-HT), and prostaglandin (PGE2).Material and Methods Ninety-nine non-small cell lung cancer (NSCLC) patients who received VATS were included in the study. All the patients received intravenous inhalation compound anesthesia. Of the 99 cases, 49 subjects (control group) received sulfentanyl for patient controlled intravenous analgesia (PICA) and other 50 cases (experiment group) received sulfentanyl combined with dexmedetomidine hydrochloride for PICA after operation of VATS. The analgesic effects of the two groups were evaluated according to Visual Analogue Scales (VAS) and the Bruggrmann Comfort Scale (BCS). The serum pain mediator of NE, DA, 5-HT, and PGE2 were examined and compared between the two groups in the first 24 h post-surgery.Results The VAS scores for the experiment group were significant lower than that of control group on the time points of 8, 16, and 24 h post-surgery (pall<0.05), and the BCS scores of the experiment group in the time points of 8, 16, and 24 h were significantly higher than that of controls (p<0.05). However, the VAS and BCS scores were not statistical differently in the time point of 1, 2, and 4 h post-surgery (pall>0.05). The mean sulfentanyl dosage was 63.01 ± 5.14 μg and 67.12 ± 6.91 μg for the experiment and control groups respectively with significant statistical difference (p<0.05). The mean analgesic pump pressing times were 4.30 ± 1.31 and 5.31 ± 1.46 for experiment and control groups respectively with significant statistical difference (p<0.05). The serum NE, DA, 5-HT, and PGE2 levels were significantly lower in the experimental group compared to that of control group in the time point of 12 h post-surgery (pall<0.05). The side effects of nausea, vomiting, delirium, rash, and hypotension atrial fibrillation were not statistically different between the two groups (pall>0.05).Conclusion Patient controlled intravenous analgesia of sulfentanyl combined with dexmedetomidine hydrochloride was effective in reducing the VAS score and serum pain mediators in NSCLC patients who received VAST.

The Effectiveness of Antibiotic-Coated Ventriculoperitoneal Shunts for Prevention of Shunt Infections in Patients with Myelomeningocele

2021 ◽  
pp. 1-4
Author(s):  
Bilal Ertuğrul ◽  
Metin Kaplan ◽  
Ömer Batu Hergünsel ◽  
Bekir Akgün ◽  
Sait Öztürk ◽  
...  
Keyword(s):  
Protective Effect ◽  
Statistical Difference ◽  
Shunt Infection ◽  
Infection Rates ◽  
Ventriculoperitoneal Shunts ◽  
Significant Statistical Difference ◽  
Shunt Infections

<b><i>Purpose:</i></b> Hydrocephalus is a common comorbidity among the newborns, with myelomeningocele (MMC) and ventriculoperitoneal (VP) shunts being frequently used for the treatment of such patients. In this study, we aimed to compare the effectiveness of antibiotic-free and antibiotic-coated shunts to reduce the rate of shunt infection in patients with hydrocephalus and accompanying MMC. <b><i>Methods:</i></b> 116 patients with hydrocephalus and MMC who were treated with VP shunts were included in the study. Shunt infection rates among antibiotic-free and antibiotic-coated shunts were compared. <b><i>Results:</i></b> Of the 116 patients included in the study, 39 had antibiotic-coated shunts and 77 had antibiotic-free shunts. Shunt infection developed in 4 of the 39 cases treated with antibiotic-coated shunts and in 5 of the 77 cases treated with shunts without antibiotics. No significant statistical difference was found between antibiotic-coated and antibiotic-free VP shunts in terms of shunt infection (<i>p</i> = 0.450, <i>p</i> &#x3e; 0.05). <b><i>Conclusion:</i></b> In patients with MMC, using VP shunts containing antibiotics was found not to have a protective effect in preventing shunt infection. Whether the sac is intact or ruptured does not affect this result.

scholarly journals The Spectrum of Bacille Calmette–Guérin Diseases in Children—A Decade of Data from Neonatal Vaccination Settings

Vaccines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 150
Author(s):  
Noora Al Busaidi ◽  
Prakash KP ◽  
Amina Al-Jardani ◽  
Nashat Al-Sukaiti ◽  
Salem Al Tamemi ◽  
...  
Keyword(s):  
Laboratory Data ◽  
International Classification Of Diseases ◽  
Bcg Vaccine ◽  
Bacille Calmette Guerin ◽  
Related Disease ◽  
Central Public Health ◽  
Classification Of Diseases ◽  
Polymerase Chain ◽  
Disseminated Disease

In this paper, we present a multicentre record-based descriptive study used to estimate the incidence and characterize the spectrum of confirmed bacille Calmette–Guérin (BCG) vaccine-related disease among children in Oman. This study included all children (age ≤ 14 years) who had culture and/or polymerase chain reaction (PCR)-confirmed BCG disease from January 2006 to December 2018, as identified from Central Public Health Laboratory data and International Classification of Diseases coding of an electronic patient information system. In total, 88 children confirmed to have BCG disease were included in the study, making an average incidence of 9.2 cases per 100,000 vaccinated neonates. The males comprised 65.9%, Omanis 93.2%, and the median age of presentation was 4 months in children with BCG disease. The most common type of disease was BCG abscesses (72.4%). Children with immunodeficiency and those presenting within 6 months were found to have a more severe and disseminated disease. In total, 28 children had immunodeficiency. The age of presentation and type of BCG disease was significantly associated with immunodeficiency status. The majority of cases required therapy (both medical and surgical) and recovered well. The incidence of laboratory-confirmed BCG vaccine-related disease was low in Oman supporting continuing the use of the BCG vaccination practice at birth.

scholarly journals Factors Enhancing Serum Syndecan-1 Concentrations: A Large-Scale Comprehensive Medical Examination

2019 ◽  
Vol 8 (9) ◽  
pp. 1320
Author(s):  
Kazumasa Oda ◽  
Hideshi Okada ◽  
Akio Suzuki ◽  
Hiroyuki Tomita ◽  
Ryo Kobayashi ◽  
...  
Keyword(s):  
Large Scale ◽  
Prospective Observational Study ◽  
Nitrogen Concentration ◽  
Laboratory Data ◽  
University Hospital ◽  
Endothelial Glycocalyx ◽  
Sample Collection ◽  
Blood Samples ◽  
Triglyceride Concentration ◽  
Medical Examinations

Endothelial disorders are related to various diseases. An initial endothelial injury is characterized by endothelial glycocalyx injury. We aimed to evaluate endothelial glycocalyx injury by measuring serum syndecan-1 concentrations in patients during comprehensive medical examinations. A single-center, prospective, observational study was conducted at Asahi University Hospital. The participants enrolled in this study were 1313 patients who underwent comprehensive medical examinations at Asahi University Hospital from January 2018 to June 2018. One patient undergoing hemodialysis was excluded from the study. At enrollment, blood samples were obtained, and study personnel collected demographic and clinical data. No treatments or exposures were conducted except for standard medical examinations and blood sample collection. Laboratory data were obtained by the collection of blood samples at the time of study enrolment. According to nonlinear regression, the concentrations of serum syndecan-1 were significantly related to age (p = 0.016), aspartic aminotransferase concentration (AST, p = 0.020), blood urea nitrogen concentration (BUN, p = 0.013), triglyceride concentration (p < 0.001), and hematocrit (p = 0.006). These relationships were independent associations. Endothelial glycocalyx injury, which is reflected by serum syndecan-1 concentrations, is related to age, hematocrit, AST concentration, BUN concentration, and triglyceride concentration.

scholarly journals PREVALENCE OF INFECTION WITH CAGA-POSITIVE HELICOBACTER PYLORI STRAINS AMONG CHILDREN AND ADOLESCENTS IN SOUTHERN BRAZIL

2014 ◽  
Vol 51 (3) ◽  
pp. 180-185 ◽  
Author(s):  
Juliana Ghisleni de OLIVEIRA ◽  
Cristina Helena Targa FERREIRA ◽  
Anna Carolina Saraiva CAMERIN ◽  
Cláudia Augustin ROTA ◽  
Luíse MEURER ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Children And Adolescents ◽  
Polymerase Chain Reaction Method ◽  
Southern Brazil ◽  
Worldwide Distribution ◽  
Clinical Presentations ◽  
Urease Test ◽  
Polymerase Chain ◽  
H Pylori ◽  
Low Prevalence

Context Helicobacter pylori (H. pylori) has a worldwide distribution, but the prevalence of infection, virulence factors, and clinical presentation vary widely according to the studied population. In Brazil, a continental country composed of several ethnicities and cultural habits, the behavior of infection also appears to vary, as many other studies have shown. Objectives Describe the prevalence of infection with cagA-positive H. pylori strains in a group of children and adolescents who underwent esophagogastroduodenoscopy in Porto Alegre, Rio Grande do Sul. Methods Fifty-four gastric biopsy specimens of children and adolescents with H. pylori infection demonstrated by histology, urease test and molecular analysis were tested for the presence of cagA positive H. pylori strains by the polymerase chain reaction method. Results he prevalence of cagA-positive H. pylori was 29.6% (95% confidence interval, 18 to 43.6%). There were no statistically significant differences in clinical or demographic characteristics or in the endoscopic and histological features of patients infected with cagA-positive strains as compared with those infected by cagA-negative strains. Conclusions he study showed a low prevalence of infection with cagA-positive H. pylori strains among children and adolescents who underwent EGD in southern Brazil, in comparison to studies conducted with children from other regions of Brazil. There was no association between the presence of cagA-positive strains and more severe clinical presentations in the studied sample.

The effect of Hyoscine Butylbromide on the rate of vaginal delivery in cases of secondary arrest of cervical dilatation: A rondomized controlled clinical trial

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Mohamed Ahmed Elkady ◽  
Dina Yahia Mansour ◽  
Alyaa Mostafa Elshamy
Keyword(s):  
Vaginal Delivery ◽  
Statistical Difference ◽  
Saline Group ◽  
Cervical Dilatation ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Hyoscine Butylbromide ◽  
Labor Time ◽  
Significant Statistical Difference ◽  
Significant Difference

Abstract Background For decades, health providers have worked to manage labor actively and safely, with the goal of shortening the duration of painful labor. Reduction of cesarean sections and maternal complication is also an important aspect of labor management. Antispasmodic are commonly administrating during labor in both developing and developed countries, although there is a paucity of scientific reports validating this. Hyoscine butylbromide is an effective antispasmodic drug. Therefore, We aim to assess efficacy of Hyoscine butylbromide on rate of vaginal delivery in cases of 2ry arrest of cervical dilatation. Objective To assess whether the intravenous injection of hyoscine butylbromide is effective in progress of labor in which cases with 2ry arrest of cervical dilatation. Patients and Methods The study included 100 pregnant women (PG, P1, P2, P3) conducted at Ain Shams University Maternity Hospital, the inclusion Criteria were: term uncomplicated singleton pregnancy in a spontaneous active labor, with cephalic presentation. The patient must be free from any medical disorder associated with pregnancy and free from any obstetric complication. Cases were subdivided into 2 groups: Study group: 50 patients received 40mg iv Hyoscine Butylbromide (as Buscopan 20mg\mrl) when the cervix &gt;4cm dilated and was &gt;50% effaced. Control group: 50 patients received 2ml saline as placebo. Results Significant increase in vaginal delivery rate (72%) in Hyoscine group; compared to Saline group (52%); with significant statistical difference (p = 0.04). Highly significant decrease in 1st and 2nd stage labor time in Hyoscine group; compared to Saline group; with highly significant statistical difference (p &lt; 0.05 respectively). These results observed without significant difference as regards 3rd stage labor time and postpartum hemorrhage (p &gt; 0.05), APGAR score and NICU admission (p &gt; 0.05). Conclusion From the current study we can conclude that HBB 40 mg is safe and effective in increasing vaginal delivary rate in cases of 2ry arrest of 12 dilatation. It is effective in decreasing first and second stage of labor. It has no maternal or neonatal adverse effect. It is easy and available.

Sign in / Sign up

Export Citation Format

Share Document