ALGORITHM OF COPROLOGICAL DI-AGNOSTICS OF INTESTINAL PROTO-SOZOSIS OF CALFS
The article presents the results of a exam-ination of calves under the age of 1 month, kept in one husbandry located on two farms in different districts of the Leningrad region. Diarrhea was found in 45% of calves at the farm in Osminovillage of, Luga district. In the Laboratory for the Study of Parasitic Diseases at the Department of Parasitology named after V. L. Yakimov at the St. Peters-burg State University of Veterinary Medi-cine, the scatological study algorithm con-sisted of making a direct smear, using the Darling’s method with an improved flotation fluid and staining fecal smears according to Romanovsky-Giemsa’s and Ziehl-Neelsen’s techniques. The staining of smears from fe-ces by the Romanovsky-Giemsa’s and Ziehl-Neelsen’s techniques made it possible to detect pathogens with a size of 3-5 microns, which are typical for Cryptosporidium sp. and Blastocystis sp. For the detection of blastocysts, the preferred diagnostic method is the Romanovsky-Giemsa staining of fecal smears, and for the detection of cryptospor-idia - Ziehl-Neelsen staining. Using this al-gorithm of scatological studies on a livestock farm located in the village of Porozhkino, the parasitic etiology of dysfunction of the gastrointestinal tract in calves was excluded. At the livestock farm in the village of Os-mino, a complex of scatological studies was carried out to identify the causative agents of cryptosporidiosis and blastocystosis, and recommendations were given on the imple-mentation of therapeutic and prophylactic measures on this farm. The diagnostic algo-rithm, which includes a step-by-step study of animal feces by the direct smear method, flotation method, staining of smears accord-ing to Romanovsky-Giemsa’s and Ziehl-Neelsen’s techniques allows us to identify a wide range of pathogens and establish not only mono, but also mixed infection