Characterization of the phenotypic diversity of tsiperifery (Piper spp.), the wild pepper from Madagascar under-studied in spite of its international fame

2020 ◽  
pp. 39-44
Author(s):  
H. Razafimandimby ◽  
L. Gautier ◽  
J.M. Bouvet ◽  
P. Danthu ◽  
L. Ramamonjisoa
Keyword(s):  
Phenotypic Diversity

scholarly journals Isolation and characterization of Anoxygenic phototrophs from shrimp ponds in Thailand

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Xelimar Ramirez ◽  
Imeleta Luamanu ◽  
Ruben Michael Ceballos ◽  
Elizabeth Padilla Crespo
Keyword(s):  
Environmental Samples ◽  
Production Systems ◽  
Phenotypic Diversity ◽  
Purple Bacteria ◽  
Shrimp Farming ◽  
Post Inoculation ◽  
Shrimp Ponds ◽  
Anoxygenic Phototrophs ◽  
Isolation And Characterization

Anoxygenic phototrophic purple bacteria are ubiquitous in aquatic and terrestrial environments and demonstrate broad phenotypic diversity. Purple bacteriaderive energy from light under anaerobic conditions via anoxygenic photosynthesis, a process in which water is not the electron donor. It has been suggested that these bacteria are useful for a variety of applications, including: wastewater treatment; heavy metal remediation; nitrogen fixation; and, control of CH4 emissions. In this study, the goal was to isolate and characterize PNSB from shrimp ponds in Thailand. Surface water and sediment were collected. Enrichment cultures were prepared using Pfenning’s mineral media. As indicated by development of reddish color and turbidity, anoxygenic phototrophic growth was observed within two days of incubation. Cultures in liquid media and on solid plates exhibited a deep red or purple color ten weeks post-inoculation. Under light microscopy, enrichments consist of communities dominated by thin, elongated gram-negative cells with granules of elemental sulfur, which are characteristic of purple bacteria. Molecular methods confirm the presence of pufLM, a genetic biomarker for purple bacteria (e.g., Thiohalocapsa marina, Allochromatium vinosum, Roseovarius tolerans). Initial sequencing of key genes (i.e., pufLM) indicate that these environmental samples contain novel isolates or “geographic variants” that have not been previously described. We have developed a few pure cultures of multiple species from these environmental samples. Since shrimp farming is a key industry in southern Thailand, the characterization of the microbial communities in these ecosystems, including anoxygenic phototrophs, will provide insights into how to maintain water quality in these food production systems.

scholarly journals Phenotypic diversity and carcass quality of creole goats with genetic potential for resistance to infection caused by worm

2018 ◽  
Vol 53 (7) ◽  
pp. 849-857
Author(s):  
Vanessa dos Santos Neri ◽  
José Lindenberg Rocha Sarmento ◽  
Laylson da Silva Borges ◽  
Tatiana Saraiva Torres ◽  
Luciano Silva Sena ◽  
...  
Keyword(s):  
Body Weight ◽  
Phenotypic Diversity ◽  
Phenotypic Characterization ◽  
Carcass Quality ◽  
Breeding Programs ◽  
Genetic Groups ◽  
Genetic Potential ◽  
Resistance To Infection

Abstract: The objective of this work was to phenotypically characterize creole goats of the Moxotó, Azul, Canindé, Repartida, Marota, and Graúna breeds, comparing them with the Boer, Anglo Nubian, and Alpine exotic breeds, to identify the genetic groups of greater potential for carcass quality and resistance to infection caused by worm. Data on morphometric measurements were obtained for the count of worm eggs in grams of feces (WEGF), Famacha score, carcass measurements, and body weight from 308 animals, in a farm in the state of Piauí, Brazil. Repartida goats showed the highest average for wither height (61.55 cm) and rump height (62.16 cm), and Moxotó goats had the greatest ear length (13.45 cm). The Moxotó breed showed the highest means for carcass and body weight. The lowest average for body weight was observed in Azul goats (24.35 kg), and the lowest WEGF was detected in Repartida goats (200 eggs per gram of feces). The average Famacha score was 2.77. Average linkage was the method that best summarized the information on the morphometric and carcass data. The use of morphometric and carcass measurements provides satisfactory results in the phenotypic characterization of the animals. The Brazilian creole goats, which are considered resistant to worm infection and show high-quality carcass traits, may be indicated for conservation and genetic breeding programs.

scholarly journals Transcriptomic characterization of microglia activation in a rat model of ischemic stroke

2020 ◽  
Vol 40 (1_suppl) ◽  
pp. S34-S48
Author(s):  
Wenjun Deng ◽  
Emiri Mandeville ◽  
Yasukazu Terasaki ◽  
Wenlu Li ◽  
Julie Holder ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Cerebral Ischemia ◽  
Phenotypic Diversity ◽  
Focal Cerebral Ischemia ◽  
Wide Spectrum ◽  
Spontaneously Hypertensive ◽  
Transient Focal Cerebral Ischemia ◽  
Microglial Response ◽  
Whole Transcriptome

Microglia are key regulators of inflammatory response after stroke and brain injury. To better understand activation of microglia as well as their phenotypic diversity after ischemic stroke, we profiled the transcriptome of microglia after 75 min transient focal cerebral ischemia in 3-month- and 12-month-old male spontaneously hypertensive rats. Microglia were isolated from the brains by FACS sorting on days 3 and 14 after cerebral ischemia. GeneChip Rat 1.0ST microarray was used to profile the whole transcriptome of sorted microglia. We identified an evolving and complex pattern of activation from 3 to 14 days after stroke onset. M2-like patterns were extensively and persistently upregulated over time. M1-like patterns were only mildly upregulated, mostly at day 14. Younger 3-month-old brains showed a larger microglial response in both pro- and anti-inflammatory pathways, compared to older 12-month-old brains. Importantly, our data revealed that after stroke, most microglia are activated towards a wide spectrum of novel polarization states beyond the standard M1/M2 dichotomy, especially in pathways related to TLR2 and dietary fatty acid signaling. Finally, classes of transcription factors that might potentially regulate microglial activation were identified. These findings should provide a comprehensive database for dissecting microglial mechanisms and pursuing neuroinflammation targets for acute ischemic stroke.

scholarly journals Agronomic, physiological and molecular characterization of spring wheat (Triticum aestivum L.) accessions for drought tolerance

2020 ◽  
Vol 49 (1) ◽  
pp. 29-38
Author(s):  
Muhammad Qadir Ahmad ◽  
Muhammad Farooq Naseer ◽  
Abdul Qayyum ◽  
Sami Ul-Allah ◽  
Waqas Malik ◽  
...  
Keyword(s):  
Drought Stress ◽  
Molecular Characterization ◽  
Genetic Association ◽  
Spring Wheat ◽  
Phenotypic Diversity ◽  
Grain Filling ◽  
Triticum Aestivum L ◽  
Kernel Weight ◽  
Chlorophyll Contents

A comprehensive study was conducted to investigate (i) the effect of drought stress on genetic association among various yield traits and (ii) molecular and phenotypic diversity in the selected spring wheat accessions. A panel of 24 spring wheat accessions was evaluated under normal irrigation and drought stress. Data were collected for chlorophyll contents, canopy temperature, days to heading, grain filling period, relative water content, plant height, peduncle length, number of kernels per spike, 1000-kernel weight and grain yield. Analysis of variance depicted significant differences for genotypes (G), effect of treatment (T) and interaction between T and G. Correlation analysis revealed that genetic association among various traits was stronger in normal treatment than water stressed. The Biplot analysis grouped the genotypes into tolerant and susceptible groups. Molecular characterization of tolerant and susceptible genotypes was done with 14 ISSR markers. ISSR primers revealed a mean of 0.63 genetic similarities among genotypes. Polymorphism information content (PIC) values varied from 0.24 to 0.49. The genetic diversity in selected germplasm can be used to develop drought tolerant lines considering the changing pattern of traits association under drought stress.

Weed diversity and weed management

Weed Science ◽  
1997 ◽  
Vol 45 (3) ◽  
pp. 357-363 ◽  
Author(s):  
Jack Dekker
Keyword(s):  
Weed Management ◽  
Phenotypic Diversity ◽  
Management Strategies ◽  
Physical Space ◽  
Cropping System ◽  
Weed Species ◽  
Plant Parts ◽  
Weed Diversity ◽  
Diversity Enhancement

The story of agriculture is the story of weed interference. After millennia of weed control we still have weeds. This situation has led many growers to observe that “the weeds always win.” One of the most important reasons weeds are so successful is their biodiversity. Biodiversity is an inevitable consequence of the struggle an individual weed species undergoes in the presence of neighbors, and by occupying a physical space in an agroecosystem. Weeds have evolved in response to cropping system practices by adapting and occupying niches left available in agroecosystems. Forces created by our cropping practices over evolutionary time have led to the weed diversity we observe today. Diversity underlies weed management in several important ways. A plant experiences diversity among its neighbors in at least five different ways. Weeds have adapted to selection in agroecosystems in several ways: (1) genetic variants within a species; (2) somatic polymorphism of plant parts; (3) success in diverse habitat microsites; (4) temporal adaptations within the community; and (5) floristic diversity of a community at higher levels than the species. Herein, weed diversity is discussed in this broader context, in terms of population behaviors that emerge as a consequence of the activities of individual components at lower levels of organization. Diversity is also discussed in terms of its implications for weed management. The potential exists to develop management strategies based on differences in weed and crop diversity. These strategies might be developed by characterization of weedy genetic and phenotypic diversity; enhancement of crop, cropping system, and agroecosystem diversity; and characterization of the spatial distribution of weed populations.

scholarly journals Phenotypic and genetic characterization of differential galacto-oligosaccharide utilization in Lactobacillus plantarum

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jori Fuhren ◽  
Markus Schwalbe ◽  
Lucía Peralta-Marzal ◽  
Christiane Rösch ◽  
Henk A. Schols ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Phenotypic Diversity ◽  
Degree Of Polymerization ◽  
Carbohydrate Utilization ◽  
Potential Health ◽  
Probiotic Strains ◽  
Health Promoting ◽  
Phenotypic And Genetic Characterization

AbstractSeveral Lactobacillus plantarum strains are marketed as probiotics for their potential health benefits. Prebiotics, e.g., galacto-oligosaccharides (GOS), have the potential to selectively stimulate the growth of L. plantarum probiotic strains based on their phenotypic diversity in carbohydrate utilization, and thereby enhance their health promoting effects in the host in a strain-specific manner. Previously, we have shown that GOS variably promotes the strain-specific growth of L. plantarum. In this study we investigated this variation by molecular analysis of GOS utilization by L. plantarum. HPAEC-PAD analysis revealed two distinct GOS utilization phenotypes in L. plantarum. Linking these phenotypes to the strain-specific genotypes led to the identification of a lac operon encoding a β-galactosidase (lacA), a permease (lacS), and a divergently oriented regulator (lacR), that are predicted to be involved in the utilization of higher degree of polymerization (DP) constituents present in GOS (specifically DP of 3–4). Mutation of lacA and lacS in L. plantarum NC8 resulted in reduced growth on GOS, and HPAEC analysis confirmed the role of these genes in the import and utilization of higher-DP GOS constituents. Overall, the results enable the design of highly-selective synbiotic combinations of L. plantarum strain-specific probiotics and specific GOS-prebiotic fractions.

scholarly journals Comprehensive description of the current breast cancer microenvironment advancements via single-cell analysis

2021 ◽  
Vol 40 (1) ◽  
Author(s):  
Xueqi Yan ◽  
Yinghong Xie ◽  
Fan Yang ◽  
Yijia Hua ◽  
Tianyu Zeng ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Phenotypic Diversity ◽  
Cell Analysis ◽  
Mass Cytometry ◽  
Comprehensive Description ◽  
Infiltrating Cells ◽  
Tumoral Cells

AbstractBreast cancer is a heterogeneous disease with a complex microenvironment consisting of tumor cells, immune cells, fibroblasts and vascular cells. These cancer-associated cells shape the tumor microenvironment (TME) and influence the progression of breast cancer and the therapeutic responses in patients. The exact composition of the intra-tumoral cells is mixed as the highly heterogeneous and dynamic nature of the TME. Recent advances in single-cell technologies such as single-cell DNA sequencing (scDNA-seq), single-cell RNA sequencing (scRNA-seq) and mass cytometry have provided new insights into the phenotypic and functional diversity of tumor-infiltrating cells in breast cancer. In this review, we have outlined the recent progress in single-cell characterization of breast tumor ecosystems, and summarized the phenotypic diversity of intra-tumoral cells and their potential prognostic relevance.

scholarly journals Morphological and reproductive characterization of nascent allotetraploids cross-compatible with cultivated peanut (Arachis hypogaea L.)

2021 ◽  
Author(s):  
Chandler Levinson ◽  
Ye Chu ◽  
Xuelin Luo ◽  
H. Thomas Stalker ◽  
Dongying Gao ◽  
...  
Keyword(s):  
Phenotypic Diversity ◽  
Phenotypic Variability ◽  
Diploid Species ◽  
Lower Percentage ◽  
Crop Breeding ◽  
Arachis Hypogaea L ◽  
Cultivated Peanut ◽  
Leaf Hairiness ◽  
Wild Diploid Species

AbstractPeanut improvement is limited by a narrow genetic base. However, this obstacle can be circumvented by incorporating phenotypic variability from wild, diploid Arachis species through interspecific hybridizations. In this study, four allotetraploid interspecific hybrids IpaCor4x (A. ipaensis × A. correntina), IpaDur4x (A. ipaensis × A. duranensis), IpaSten4x (A. ipaensis × A. stenosperma), and ValSten4x (A. valida × A. stenosperma) were created and morphologically characterized through the following parameters: flower count, flower size, flower banner pigmentation, leaf area and weight, leaf hairiness, main stem height, internode length, percent of reproductive nodes, biomass, 100 pod weight, and 100 seed weight. For every trait, except for flower banner absorption at 380 nm, at least one or more allotetraploids differed from the cultivated peanut control. In general, these allotetraploids had a greater production of flowers during the growing season, larger flowers, larger and hairier leaves, taller main stems, longer primary laterals, longer internodes, lower percentage of reproductive nodes, heavier plant body masses, and smaller seeds and pods. This phenotypic diversity can be utilized directly in ornamental and forage breeding, while for oil and food crop breeding, this diversity will likely need to be selected against while desirable traits such as disease and insect resistance and abiotic stress tolerances derived from the wild diploid species are maintained.

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