In vitro toxicity of MTA Compared with other Primary Teeth Pulpotomy Agents

2009 ◽  
Vol 33 (3) ◽  
pp. 217-222 ◽  
Author(s):  
José Vitor Nogara Borges de Menezes ◽  
Esther Rieko Takamori ◽  
Maria Francisca Thereza Borro Bijella ◽  
José Mauro Granjeiro
Keyword(s):  
Primary Teeth ◽  
Statistical Tests ◽  
Neutral Red ◽  
The Other ◽  
In Vitro Toxicity ◽  
Ferric Sulphate ◽  
Significant Difference ◽  
Other Substances ◽  
Neutral Red Uptake

Objective: The main goal of this work is to compare the In vitro toxicity of MTA with other primary teeth pulpotomy agents. Study Design: The In vitro toxicity of MTA, calcium hydroxide, ferric sulphate solution,diluted formocresol and Buckley's formocresol were tested using MTT and Neutral Red Uptake cell viability assays. The results for MTA were compared to those obtained for the other substances using ANOVA and Tukey statistical tests (p<0,05). Results: MTA had the lower in vitro toxicity and Buckley's formocresol, the higher, with statiscally significant difference. Conclusion: Among the primary teeth pulpotomy agents tested, MTA showed the lower In vitro toxicity, standing as the most promising substitute to formocresol.

Effect of Different Intermediary Bases on Microleakage of a Restorative Material in Class II Box Cavities of Primary Teeth

2017 ◽  
Vol 40 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Faika Y. Abdelmegid ◽  
Fouad S. Salama ◽  
Waleed M. Al-Mutairi ◽  
Saud K. Al-Mutairi ◽  
Sultan O. Baghazal
Keyword(s):  
Primary Teeth ◽  
In Vitro Study ◽  
Class Ii ◽  
Positive Control ◽  
Negative Control ◽  
The Other ◽  
Control Groups ◽  
Significant Difference ◽  
The Mean

Introduction The aim of this in vitro study was to assess and compare the effect of different intermediary bases on microleakage between tooth and a nanocomposite interface in Class II box cavities in primary teeth. Methods Standard Class II box cavities were prepared in 52 primary molars and randomly divided into 9 groups according to the intermediary base used (Multicore Flow, Fuji II LC, SDR, Smart Dentin Replacement, and Biodentine). All specimens were subjected to thermocycling and prepared for microleakage testing and evaluation. Results There was significant difference in the mean ranks of microleakage between the 9 groups, which was observed in the gingival side (p<0.0001) and the occlusal side (p<0.0001). The mean ranks microleakage was significantly higher with experimental SDR, experimental Multicore Flow, and positive control materials when compared with the other 6 groups. The microleakage mean ranks were statistically significantly lower in experimental Fuji II LC, experimental Biodentine, and all negative control groups when compared with the other 3 groups. Conclusions Microleakage is affected by the application of intermediate material. Experimental Biodentine and Fuji II LC showed the lowest microleakage while experimental SDR and experimental Multicore Flow showed the highest microleakage.

The EYTEX™ System and the Neutral Red Uptake Inhibition Assay in Cultured Hep G2 Cells as Alternative Methods for In Vivo Eye Irritation Following the EEC Protocol

1990 ◽  
Vol 17 (4) ◽  
pp. 325-333
Author(s):  
Paul J. Dierickx ◽  
Virginia C. Gordon
Keyword(s):  
Neutral Red ◽  
Alternative Methods ◽  
Inhibition Assay ◽  
Hep G2 ◽  
Hep G2 Cells ◽  
Eye Irritation ◽  
Uptake Inhibition ◽  
In Vitro Methods ◽  
Neutral Red Uptake

The neutral red uptake inhibition assay and the EYTEX™ system were investigated as alternative methods for the assessment of eye irritation, determined according to the EEC protocol. The 17 test chemicals used were mainly organic solvents. The xenobiotics were applied to Hep G2 cells for 24 hours at different concentrations. Neutral red uptake inhibition was then measured. The results are expressed as the NI50 value, which is the concentration of test compound required to induce a 50% reduction in neutral red uptake. The same chemicals were also tested as coded samples by the EYTEX™ test according to the manufacturer's directions. A nearly identical quantitative correlation was found for both in vitro methods with corneal opacity scores: r = 0.84 for EYTEX™ scores and r = 0.83 for log NI50, expressed in μg/ml. Whilst these correlations are certainly not perfect, it is clear that both in vitro methods can be used as valuable prescreening methods.

scholarly journals Bond Strength of Self-Adhesive Flowable Composites and Glass Ionomer Cements to Primary Teeth: A Systematic Review and Meta-Analysis of In Vitro Studies

Materials ◽  
2021 ◽  
Vol 14 (21) ◽  
pp. 6694
Author(s):  
Flavia Iaculli ◽  
Alessandro Salucci ◽  
Gianni Di Giorgio ◽  
Valeria Luzzi ◽  
Gaetano Ierardo ◽  
...  
Keyword(s):  
Systematic Review ◽  
Bond Strength ◽  
Primary Teeth ◽  
Meta Analysis ◽  
Deciduous Teeth ◽  
Glass Ionomer Cements ◽  
Glass Ionomer ◽  
Significant Difference ◽  
Flowable Composites

Background: Conventional composites are largely used in pediatric restorative dentistry and demonstrate successful clinical outcomes. However, the need for simplification of operative steps in young or uncooperative children demands reliable alternatives. Therefore, the aim of the present systematic review and meta-analysis was to evaluate the in vitro bond strength of glass ionomer cements (GICs) and self-adhesive flowable composites (SFCs) on deciduous teeth. Methods: A comprehensive literature search according to the PRISMA checklist was manually and electronically performed by two independent reviewers through the following databases: MEDLINE/PubMed, Google Scholar, Scopus, and Embase, to include in vitro studies comparing GICs and SFCs bond strength values of restorations on primary teeth. In addition, three groups of meta-analyses were conducted using random-effects models. Results: Three articles meeting the inclusion criteria were selected and subjected to both qualitative and quantitative assessment. No statistically significant difference was found between SFC versus GIC; however, both groups significantly differed with conventional flowable composites (CFs). Conclusions: Despite the absence of significant difference in bond strength values, SFCs may be considered a valid alternative to GICs in the restoration of deciduous teeth, although CFs proved better in vitro performances.

scholarly journals Pengaruh Gambut Terbakar Terhadap Beberapa Sifat Fisika Gambut di Kecamatan Tripa Makmur

2020 ◽  
Vol 4 (2) ◽  
pp. 416-425
Author(s):  
Teuku Muhammad Afrizal ◽  
Hairul Basri ◽  
Muhammad Rusli Alibasyah
Keyword(s):  
Organic Matter ◽  
Physical Properties ◽  
Water Content ◽  
Statistical Tests ◽  
Peat Soil ◽  
Rate Of Change ◽  
The Other ◽  
Volume Weight ◽  
Top Soil ◽  
Significant Difference

Abstrak. Gambut mengandung bahan organik yang tinggi. Gambut yang didrainase rentan terhadap kebakaran. Pemanfaatan gambut di Aceh sangat luas. Umumnya, gambut sering dibakar agar dapat dengan cepat dimanfaatkan., khususnya di Kecamatan Tripa Makmur Kabupaten Nagan Raya. Penelitian ini bertujuan untuk melihat seberapa jauh tingkat perubahan sifat fisika gambut terbakar Rawa Tripa Kabupaten Nagan Raya. Hasil penelitian menunjukkan bahwa rata-rata berat volume tertinggi terdapat pada sampel T1 (top soil) yaitu  0,36 g cm-3 dan terendah pada T0 (sub soil) yaitu  0,17 g cm-3. Kadar air tertinggi  terdapat pada sampel T0 (sub soil)  yaitu 234,82% dan terendah pada T1 (top soil) yaitu 81,96%. Porositas tertinggi terdapat pada sampel gambut T0  (sub soil) yaitu 94,93% dan terendah pada T1 (top soil) yaitu 65,28%. Permeabilitas tertinggi terdapat pada T0 (sub soil) yaitu 33,80 cm/jam dan terendah pada T1 (top soil) yaitu 16,10 cm/jam. Hasil uji statistik menunjukkan bahwa ada perbedaan yang signifikan pada parameter berat volume dengan t hitung (3,482) t tabel (1, 943), sedangkan pada parameter lainnya tidak ada perbedaan yang signifikan. Tingkat kematangan gambut di kawasan Tripa Makmur adalah hemik. Kedalaman gambut sebesar 3 meter.  Subsiden tertinggi adalah 100 cm pada gambut terbakar di tahun 2015. Warna  gambut adalah coklat kehitaman. The Effect of Burning Peat on Some of The Physical Porperties of Peat Soil in The Tripa Makmur Sub DistrictAbstract. Peat contains high organic matter. Drained peat is susceptible to fire. The use of peat in Aceh is very broad. Peat is often burned so that it can be quickly utilized especially in Tripa makmur Sub Districk, Nagan Raya Districk. This study aims to see how far the rate of change in the physical properties of peat is burning Rawa Tripa in Nagan Raya District. The results showed that the highest average of volume weight was found in T1(top soil) 0,36 g cm-3 and the lowest in T0(sub soil) 0,17 g cm-3. The highest water content was found inT0(sub soil) 234,82%  and the lowest was on T1(top soil) 81,96%. The highest porosity was found T0(sub soil) 94,93% and the lowest in T1(top soil) 65,28%. The highest permeability is found in T0(sub soil) 33,80 cm/hour and the lowest is on T1(top soil) 16,10 cm/hour. The results of statistical tests show that there is a significant difference in the parameters of volume weight with t count 3,482 t table 1,943, while in the other parameters have no significant differences. The level of maturity in the Tripa Makmur area is hemik. Peat depth of 3 meters. The highest subsidance is 100 cm on burning peat in 2015. The color of this peat is blackish brown. 

scholarly journals The in vitro toxicity of atrazine on kinematics and DNA fragmentation in common carp (Cyprinus carpio) sperm cells

2019 ◽  
Vol 70 (3) ◽  
pp. 1639
Author(s):  
M.E. ÖZGÜR
Keyword(s):  
Dna Fragmentation ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
In Vitro Toxicity ◽  
Computer Assisted ◽  
Sperm Cells ◽  
Common Carp Cyprinus Carpio ◽  
Significant Difference ◽  
Carp Cyprinus Carpio

This study investigated the in vitro effects of different concentrations of Atrazine (0.001, 0.01, 0.1, 0.5 mg/L) added to motile and immotile solutions on kinematics quality of sperm cells of common carp, Cyprinus carpio, which is a fish of economic significance. The kinematics of the sperm cells was analyzed by a computer-assisted sperm analysis system (CASA). As a result of the study, while there was a significant difference (P < 0.05) between the groups in terms of the VSL (μm/s) and VCL (μm/s) values after the Atrazine-added immotile solution’s (IMS) and incubation for 3 hours, there was a significant difference (P < 0.05) in only the VSL values directly activated by the Atrazine-added motile solution (MS). DNA fragmentation was evident but not in higher numbers in the 0.1 mg/L atrazine group. Finally, it was determined the effective concentration (EC50) values of the VSL value of the motile and immotile solution as 0.34 mg/L and 0.03 mg/L, respectively.

scholarly journals Effect of Er,Cr:YSGG on Remineralization Using CPP - ACPF (MI - Paste Plus) after Enamel Erosion Caused by Carbonated Soft Drink in Primary Teeth: In-Vitro Study

2019 ◽  
Vol 7 (7) ◽  
pp. 1184-1192 ◽  
Author(s):  
Ghada Ezzeldin Elwardani ◽  
Tarek Abdel Hamid Harhash ◽  
Ahmed Abbas Zaky
Keyword(s):  
Primary Teeth ◽  
Artificial Saliva ◽  
Soft Drink ◽  
Combined Treatment ◽  
Laser Application ◽  
Mi Paste Plus ◽  
Group I ◽  
Significant Difference ◽  
Mi Paste

BACKGROUND: Erosion is a widespread phenomenon with higher predilection in primary dentition. AIM: The aim of the present study is to assess the remineralising effect of Er,Cr:YSGG laser application combined with CPP-ACPF after erosive demineralisation by Coca-Cola in primary teeth. METHODS: Fifty teeth (n = 10) were divided into; Group I: Artificial saliva, (Saliva natural, Medac, UK), Group II: CPP-ACPF (MI Paste Plus, GC Corp, USA), Group III: Er,Cr:YSGG (Waterlase iPlus, USA), Group IV: CPP-ACPF + Er,Cr:YSGG, Group V: Er,Cr:YSGG + CPP-ACPF. Teeth were immersed in Coca-Cola for 10 min, 5 times/day for 5 days. DIAGNOdent (DD) measurements were taken before and after the experiment. RESULTS: There was a significant increase in DD readings after erosive-treatment cycles in all test groups. The highest reading was in samples immersed in artificial saliva, and the lowest was in those subjected to combined CPP-ACPF and Er,Cr:YSGG laser application, regardless of the sequence used. There was no significant difference between samples immersed in artificial saliva, and after CPP-ACPF application. Similarly, there was no significant difference between samples treated by combined treatment of CPP-ACPF and Er,Cr:YSGG application. However, there was a significant difference between samples immersed in artificial saliva or treated with CPP-ACPF application and those subjected to combined treatment CPP-ACPF along with Er,Cr:YSGG. CONCLUSION: Combining Er,Cr:YSGG laser and CPP-ACPF paste significantly increased enamel remineralisation, regardless of the sequence implemented. Saliva naturally and CPP-ACPF application had a comparable effect on remineralisation.

Angiopoietin-1 and Osteopontin Expression by CD138+ Myeloma Cells Rather Than VEGF and CD45 Correlates with Bone Marrow Angiogenesis in Multiple Myeloma Patients at the Diagnosis.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 2501-2501
Author(s):  
Nicola Giuliani ◽  
Simona Colla ◽  
Francesca Morandi ◽  
Sabrina Bonomini ◽  
Mirca Lazzaretti ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Linear Trend ◽  
The Other ◽  
Vegf Mrna ◽  
Myeloma Cells ◽  
Other Hand ◽  
Significant Difference ◽  
Angiopoietin 1

Abstract Bone marrow (BM) angiogenesis is increased in Multiple Myeloma (MM) patients and correlates with disease progression and patient survival. Myeloma cells secrete the main endothelial growth factor VEGF. In mouse models VEGF secretion as well as the angiogenic properties of MM cells correlate with the lack of CD45 expression by MM cells. However, recent data indicate that VEGF plasma cell expression is similar between MGUS and MM patients suggesting that other molecules could be involved. In line with this hypothesis we have recently demonstrated that myeloma cells may also produce factors with angiogenic properties as angiopoietin-1 (ANG-1) and osteopontin (OPN) that are involved in myeloma induced angiogenesis in vitro. In order to identify which factors correlate with BM angiogenesis in MM patients, we have investigated in a cohort of 121 newly diagnosed MM patients (stage I–III) the expression of the angiogenic molecules VEGF, ANG-1 and OPN and their correlation with bone marrow (BM) angiogenesis and CD45 expression by MM cells. We found that 90% of CD138+ MM cells tested were positive for VEGF mRNA. On the other hand we found that 50% and 40 % of MM patients were positive for ANG-1 and OPN mRNA respectively. Using the previously published cut off for CD45 expression we found that 61 out of 121 MM patients were positive for CD45 and 60 out of 121 were negative for CD45 expression. Any correlation was not observed between VEGF expression and BM angiogenesis in MM patients (p=0.5), whereas the number of microvessels X field was higher in Ang-1 positive patients in comparison with Ang-1 negative ones (mean±SE: 6.23±0.2 vs. 2.94±0.1, median: 6.21 vs. 2.79; p=0.001,) and the microvascular density (MVD) was significantly increased (32.98±1.7 vs. 14.55±1.3, median: 34.69 vs. 13.04; p&lt;0.01; capillaries: 26.73±1.3 vs. 10.42±0.8, median: 24.06 vs. 9.04; p&lt;0.01, small venules: 9.56 ±0.5 vs. 4.14±0.5, median: 10.60 vs. 3.65; p&lt;0.01). Furthermore a significantly positive correlation between Ang-1 expression and MVD was found (Pearson Chi-square: p=0.036, Cochran’s Linear Trend: p=0.01). A significantly higher MVD was also observed in the group of patients positive for OPN, (mean±SE: 29.1±0.7 vs. 17.55±0.37; p&lt;0.01) and similarly, the number of microvessels per field was higher in OPN positive patients in comparison with OPN negative ones (mean±SE: 6.7±0.15 vs. 4.28±0.04; p=0.05). On the other hand, any significant difference was not observed between CD45 positive and CD45 negative patients for the expression of VEGF (p=0.4), ANG-1 (p=0.3) and OPN (p=0.09). Consistently we did not find any significant difference in both MVD and number of vessels X field between CD45 positive patients as compared with CD45 negative ones (p=0.5 and p=0.4, respectively). Finally, a multivariate analysis confirmed that VEGF and CD45 did not correlate with the BM angiogenesis showing that ANG-1 expression by MM cells was more tightly correlated with MVD and the number of vessels X field as compared to OPN. Our data indicate that ANG-1 and in part OPN rather than VEGF and CD45 expression by MM cells are the critical determinants correlated with the increase of BM angiogenesis that occurs in MM patients at the diagnosis.

scholarly journals Differential toxicities of albendazole and its two main metabolites to Balb/c 3T3, HepG2, and FaO lines and rat hepatocytes

2016 ◽  
Vol 60 (4) ◽  
pp. 495-500 ◽  
Author(s):  
Lidia Radko ◽  
Maria Minta ◽  
Sylwia Stypuła-Trębas
Keyword(s):  
Hepatoma Cell ◽  
Rat Hepatocytes ◽  
Neutral Red ◽  
In Vitro Toxicity ◽  
Isolated Rat Hepatocytes ◽  
Hepatoma Cell Lines ◽  
Cellular Models ◽  
The Impact ◽  
Isolated Rat

Abstract Introduction: The cytotoxicity of anthelmintic agent, albendazole (ABZ) and its two major metabolites, sulfoxide (ABZSO) and sulfone (ABZ-SO2), on non-hepatic Balb/c 3T3 line, two hepatoma cell lines (FaO, HepG2), and isolated rat hepatocytes was investigated. Material and Methods: Cell cultures were exposed for 24, 48, and 72 h to eight concentrations of the compounds ranging from 0.05 to 100 μg/mL (ABZ) and from 0.78 to 100 μg/mL (ABZ-SO and ABZ-SO2). Three different assays were applied in which various biochemical endpoints were assessed: lysosomal activity - neutral red uptake (NRU) assay, proliferation - total protein contents (TPC) assay and lactate dehydrogenase (LDH) leakage assay. Results: The most toxic was albendazole whose EC50 values calculated from the concentration effect curves ranged from 0.2 to 0.5 μg/mL (Balb/c 3T3 ) and from 0.4 to 73.3 μg/mL (HepG2). Rat hepatoma line and isolated rat hepatocytes were less sensitive to the impact of ABZ. Toxic action expressed as EC50 was recorded after 72 h exposure only in LDH release assay at 0.8 μg/mL and 9.7 μg/mL respectively. The toxicity of metabolites was much lower. The most sensitive to ABZ-SO were fibroblasts and EC50-72h values were similar in all three assays used, i.e. NRU (14.1 μg/mL), TPC (15.8 μg/mL), and LDH (20.9 μg/mL). In the case of ABZ-SO2 the mean effective concentrations were the highest, and could be reached only in one LDH assay. These values (μg/mL) were as follows: 65.3 (FaO), 65.4 (HepG2), 75.8 (hepatocytes), and 77.4 (Balb/c 3T3). Conclusion: The differences in in vitro toxicity of albendazole depend on metabolic ability of the cellular models. Primary cultured rat hepatocytes represent a valuable tool to study the impact of biotransformation on the cytotoxicity of drugs.

The Effect of Casein Phosphopeptide-Amorf Calcium Phosphate and Acidulated Phosphate Fluoride Gel on Dental Erosion in Primary Teeth: An in Vitro Study

2017 ◽  
Vol 41 (4) ◽  
pp. 275-279 ◽  
Author(s):  
Eda Arat Maden ◽  
Özge Acar ◽  
Ceyhan Altun ◽  
Günseli Güven Polat
Keyword(s):  
Surface Roughness ◽  
Protective Effect ◽  
Primary Teeth ◽  
Artificial Saliva ◽  
Soft Drink ◽  
Dental Erosion ◽  
The Other ◽  
The Mean ◽  
Casein Phosphopeptide

Objective: This study aimed to investigate the effect of acidulated phosphate fluoride (APF) gel and casein phosphopeptide/amorphous calciumphosphate (CPP-ACP) on the dental erosion produced by carbonated soft drink in primary teeth. Study Design: This study evaluated by an in vitro model the effect of APF gel and CPP-ACP on the dental enamel previously subjected to erosive challenge with carbonated soft drink. Sixty sound human primary molars were prepared by embedding the crown sections in acrylic resin blocks leaving the enamel surfaces exposed. The surface roughness of the enamel was measured with prophilometry at baseline. Specimens were randomly divided into three treatment groups (n:20): artificial saliva, CPP-ACP, 1.23% APF gel. All specimens were then exposed to an erosive challenge of carbonated soft drink and artificial saliva for 20 cycles of 20 seconds each. Demineralization-remineralization cycles was repeated twice at eight-hour intervals and roughness values were measured. Enamel samples were treated with artificial saliva, CPP-ACP, 1.23% APF gel applied for 10 min after erosive challenge. The arithmetic average roughness (Ra) readings were recorded after remineralization agents were applied. Results: The mean surface roughness in all groups increased significantly after erosion process and decreased after remineralization treatment. After treatment, the mean surface roughness of the 1.23% APF gel group was significantly less than the other groups and the mean surface roughness of the artificial saliva group was significantly more than the other groups. 1.23% APF gel showed the highest protective effect against erosive enamel loss. Conclusions: Under the conditions of this study, artificial saliva, CPP-ACP and 1.23% APF treatments were able to reduce erosive enamel loss produced by carbonated soft drink in primary teeth. However, 1.23% APF gel showed the highest protective effect against erosive enamel loss.

scholarly journals Cytotoxic Effect of Addition of Different Concentrations of Nanohydroxyapatite to Resin Modified and Conventional Glass Ionomer Cements on L929 Murine Fibroblasts

2021 ◽  
Author(s):  
Parisa Golkar ◽  
Ladan Ranjbar Omrani ◽  
Shide Zohourinia ◽  
Elham Ahmadi ◽  
Faeze Asadian
Keyword(s):  
Cytotoxic Effect ◽  
In Vitro Study ◽  
Biological Properties ◽  
The Other ◽  
Glass Ionomer Cements ◽  
Glass Ionomer ◽  
Beneficial Effects ◽  
Significant Difference ◽  
Murine Fibroblasts

Objectives: In this study we assessed the cytotoxic effect of nanohydroxyapatite (NHA) incorporated into resin modified and conventional glass ionomer cements (RMGICs and CGICs) on L929 murine fibroblasts. Materials and Methods: In this in vitro study, 0wt%, 1wt%, 2wt%, 5wt%, 7wt% and 10wt% concentrations of NHA were added to Fuji II LC RMGIC and Fuji IX CGIC powders. Eighteen samples (5×3mm) were fabricated from each type of glass ionomer, in six experimental groups (n=3): CG0, CG1, CG2, CG5, CG7, CG10, RMG0, RMG1, RMG2, RMG5, RMG7, and RMG10. Samples were incubated for 72h. The overlaying solution was removed and added to L929 fibroblasts. The methyl thiazolyl tetrazolium bromide (MTT) assay was performed at 24, 48 and 72h. The wavelength was read by a spectrophotometer. Data were analyzed by ANOVA and Tukey’s test. Results: There was no significant difference in cytotoxicity of the two types of glass ionomers, with and without NHA, except for CG0 and RMG0 groups after 72h. RMG0 group was significantly more cytotoxic than the CG0 group (P<0.05). In CG groups during the first 24h, the cytotoxicity of CG5 and CG7 groups was significantly higher than that of CG1; while, there was no significant difference between the RMG groups. Cytotoxicity significantly decreased in all groups after 24h (P<0.05). Conclusion: Incorporation of NHA into Fuji II LC RMGIC and Fuji IX CGIC did not affect their biocompatibility and therefore its addition to these materials can provide favorable biological properties, especially considering its beneficial effects on the other properties of GICs.

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