Processed Dairy Beverages pH Evaluation: Consequences of Temperature Variation

2009 ◽  
Vol 33 (4) ◽  
pp. 319-323 ◽  
Author(s):  
Fabiana Vargas Ferreira ◽  
Roselaine Terezinha Pozzobon
Keyword(s):  
Temperature Variation ◽  
Room Temperature ◽  
Critical Level ◽  
Fermented Milk ◽  
Significance Level ◽  
Ph Values ◽  
Temperature Interaction ◽  
Fermented Dairy ◽  
Dairy Beverages

Objective: this study assessed the pH from processed dairy beverages as well as eventual consequences deriving from different ingestion temperatures. Study design: 50 adults who accompanied children attended to at the Dentistry School were randomly selected and they answered a questionnaire on beverages. The beverages were divided into 4 groups: yogurt (GI) fermented milk (GII), chocolate-based products (GIII) and fermented dairy beverages (GIV). They were asked which type, flavor and temperature. The most popular beverages were selected, and these made up the sample. A pHmeter Quimis 400 A device was used to verify pH. The average pH from each beverage was calculated and submitted to statistical analysis (Variance and Tukey test with a 5% significance level). Results: for groups I, II and III beverages, type x temperature interaction was significant, showing the pH averages were influenced by temperature variation. At iced temperatures,they presented lower pH values, which were considered statistically significant when compared to the values found for the same beverages at room temperature. Conclusion: all dairy beverages, with the exception of the chocolate-based type presented pH below critical level for enamel and present corrosive potential; as to ingestion temperature, iced temperature influenced pH reducing its values, in vitro.

In Vitro Interaction between Venous Blood Clots and Radiopharmaceuticals

1985 ◽  
Vol 24 (04) ◽  
pp. 173-179 ◽  
Author(s):  
B. R. R. Persson ◽  
V. Kempi
Keyword(s):  
Room Temperature ◽  
Venous Blood ◽  
Gel Chromatography ◽  
Time Intervals ◽  
Ph Values ◽  
Blood Clots ◽  
Glass Tubes ◽  
Ph Range ◽  
In Vitro Interaction

SummaryClots of 1 ml venous blood formed in glass tubes after 10 min at room temperature were incubated at 37° C with the radiopharmaceutical to be studied. Methods for quality control of the radiopharmaceuticals were compared. Gel chromatography scanning was found to give reliable information. The incorporation into the clot was studie’d at different pH values and after various time intervals. The highest incorporation was found for 125I-fibrinogen and for 99mTc-mac-roaggregates of albumin, followed by 99mTc-sulphur colloid and 99mTc-strep-tokinase at pH less than 2. The titrated initial dose of 99mTc-streptoki-nase was studied at various pH levels. The lysing effect was less in the pH range 1-2.5, where the best labeling yield was obtained. The inactivation of streptokinase by the labeling procedure was also studied with im-munoelectrophoresis and decomposition of casein. In vitro studies of the interaction of radiopharmaceuticals with clots add information for the clinical use of radiopharmaceuticals for thrombus localization.

scholarly journals ИСЭГ ЦАГААН ИДЭЭНЭЭС ЯЛГАСАН СҮҮН ХҮЧЛИЙН БАКТЕРИЙН ШИНЖ ЧАНАРЫН СУДАЛГАА

2017 ◽  
pp. 44-55
Author(s):  
Хандсүрэн Б ◽  
Дэмбэрэл Ш ◽  
Дүгэрсүрэн Ж
Keyword(s):  
Lactic Acid ◽  
Functional Food ◽  
In Vitro Study ◽  
Fermented Milk ◽  
Lactobacillus Helveticus ◽  
Probiotic Properties ◽  
Bacterial Strains ◽  
Intestinal Pathogens ◽  
Fermented Dairy

We isolated lactic acid bacteria from fermented dairy products, fermented mare’s milk and cow yogurt, which are traditionally produced by Mongolian herders, and carried out in vitro study of their probiotic properties. Culture 44c is 100% identical to species Lactobacillus helveticus (Lhelveticus) or a strain KT368987, isolated from Mongolian airag, while culture 65b is 99% identical to Lactobacillus delbreuckii subsp. bulgaricus (L.bulgaricus) or strain CP016393 isolated from camel fermented milk in Gobi-Altai aimag and characteristics of both cultures were the best among all cultures obtained in the present study. Isolated lactic acid rods completely inhibited the growth of gastro-intestinal pathogens which spread among livestock (E.coli 09, E.coli 026, S.abortusovis 0068) and Mongolian population (S.aureus 5695, S.aureus 5068, S.aureus SA27, E.coli 10963, E.coli 10977). As well as, we determined the properties, including resistance to the acidic environment of digestive tract and activity when using with common antibiotics, which are the basic requirements for bacterial strains used in functional food production. According to the result, when pH of the environment reached 3.5 or 4.0, growth rate of the strains was over 80 percent and the lactic acid strains were variably resistant to 10 antibiotics of 5 classes (penicillin, aminoglycoside, cephalosporin and macrolide). For example; Formation of 8.9±0.87 mm zone around all antibiotic discs for culture 44c of L.helveticus demonstrates the culture can be used in combination with these antibiotics for therapeutic purposes. Besides of these characteristics, CFU per ml was determined by growing on MRS nutrient medium under anaerobic condition. Moreover, CFU/ml was determined because its spectrum of activity depends on the number of bacteria per 1ml. L.helveticus 44c reached its highest volume (2503х1010) at 48th hour of growth, while L.bulgaricus 65b reached its highest volume (2503х1010) at 72th hour of growth. After they reached their highest volume, both of them tended to decrease. For this reason, these strains, especially L.helveticus 44c and L.bulgaricus 65b strains, are possible to be used as a source material for probiotics or functional food products.

scholarly journals Kemampuan Susu Fermentasi Lactobacillus plantarum Menghambat Salmonella typhymurium Secara In Vitro

2010 ◽  
Vol 10 (2) ◽  
pp. 34-39
Author(s):  
Zuraida Hanum
Keyword(s):  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Room Temperature ◽  
Fermented Milk ◽  
Activity Test ◽  
Milk Fermentation ◽  
Temperature Observation ◽  
Plain Paper ◽  
Paper Disc

The ability of Lactobacillus plantarum fermented milk to inhibit Salmonella typhymurium in vitroABSTRACT. This research used Lactobacillus plantarum as a milk starter (concentration 3, 4, 5 %) and incubated for 48 hours at room temperature. Observation fermented begin from the first day of this product until 7 days and its still stored at room temperature. First analysis conducted on milk in this research were Storch, pH, acidity titration, fat, and dry matter. Milk fermentation analysis of Lactobcillus plantarum including pH, degree of dornik acidity and microbe activity test. Experimental design used is Repeated Measurement with three replications. Data colected analyzed by ANOVA test. If there is significant different between treatments, followed by Least Significant Test. Complete pasteurization result test (Storch) found that milk stay white, it means that peroksidase enzyme completely disappear and milk completely pasteurization. The ability of suppressing observed by Salmonella typhymurium in Nutrient Agar and challenge with Lactobacillus plantarum fermented milk 3%, 4% and 5% (50 μl/well). Tetracyline, chloramphenicol and a plain paper disc are used as control. Lactobacillus plantarum fermented milk of starter concentration 5%-first day has the biggest inhabitation zone by Salmonella typhimurium (9.39 mm). Range of pH showed between 4.84 to 4.14 and the acidity between 114.67 0D to 365.67 0D. Sensitivity test showed that Salmonella typhymurium more sensitive than chloramphenicol and tetracyline antibiotic.

In Situ Localization of PPAR Gamma and Uncoupling Protein in Mouse Embryo Sections Using Digoxigenin-Labeled Riboprobes

1996 ◽  
Vol 54 ◽  
pp. 32-33
Author(s):  
C. Jennermann ◽  
S. A. Kliewer ◽  
D. C. Morris
Keyword(s):  
Alkaline Phosphatase ◽  
Room Temperature ◽  
Uncoupling Protein ◽  
Ppar Gamma ◽  
Nuclear Hormone Receptor ◽  
Full Length ◽  
Northern Analysis ◽  
Peroxisome Proliferator

Peroxisome proliferator-activated receptor gamma (PPARg) is a member of the nuclear hormone receptor superfamily and has been shown in vitro to regulate genes involved in lipid metabolism and adipocyte differentiation. By Northern analysis, we and other researchers have shown that expression of this receptor predominates in adipose tissue in adult mice, and appears first in whole-embryo mRNA at 13.5 days postconception. In situ hybridization was used to find out in which developing tissues PPARg is specifically expressed.Digoxigenin-labeled riboprobes were generated using the Genius™ 4 RNA Labeling Kit from Boehringer Mannheim. Full length PPAR gamma, obtained by PCR from mouse liver cDNA, was inserted into pBluescript SK and used as template for the transcription reaction. Probes of average size 200 base pairs were made by partial alkaline hydrolysis of the full length transcripts. The in situ hybridization assays were performed as described previously with some modifications. Frozen sections (10 μm thick) of day 18 mouse embryos were cut, fixed with 4% paraformaldehyde and acetylated with 0.25% acetic anhydride in 1.0M triethanolamine buffer. The sections were incubated for 2 hours at room temperature in pre-hybridization buffer, and were then hybridized with a probe concentration of 200μg per ml at 70° C, overnight in a humidified chamber. Following stringent washes in SSC buffers, the immunological detection steps were performed at room temperature. The alkaline phosphatase labeled, anti-digoxigenin antibody and detection buffers were purchased from Boehringer Mannheim. The sections were treated with a blocking buffer for one hour and incubated with antibody solution at a 1:5000 dilution for 2 hours, both at room temperature. Colored precipitate was formed by exposure to the alkaline phosphatase substrate nitrobluetetrazoliumchloride/ bromo-chloroindlylphosphate.

Evaluation of 99mTc-Label led Vitamin K4 as an Agent for Testicular Imaging

1991 ◽  
Vol 30 (01) ◽  
pp. 35-39 ◽  
Author(s):  
H. S. Durak ◽  
M. Kitapgi ◽  
B. E. Caner ◽  
R. Senekowitsch ◽  
M. T. Ercan
Keyword(s):  
Soft Tissue ◽  
Room Temperature ◽  
Normal Subjects ◽  
Left Testis ◽  
Wide Range ◽  
Activity Ratios ◽  
The Right ◽  
Radioactivity Levels

Vitamin K4 was labelled with 99mTc with an efficiency higher than 97%. The compound was stable up to 24 h at room temperature, and its biodistribution in NMRI mice indicated its in vivo stability. Blood radioactivity levels were high over a wide range. 10% of the injected activity remained in blood after 24 h. Excretion was mostly via kidneys. Only the liver and kidneys concentrated appreciable amounts of radioactivity. Testis/soft tissue ratios were 1.4 and 1.57 at 6 and 24 h, respectively. Testis/blood ratios were lower than 1. In vitro studies with mouse blood indicated that 33.9 ±9.6% of the radioactivity was associated with RBCs; it was washed out almost completely with saline. Protein binding was 28.7 ±6.3% as determined by TCA precipitation. Blood clearance of 99mTc-l<4 in normal subjects showed a slow decrease of radioactivity, reaching a plateau after 16 h at 20% of the injected activity. In scintigraphic images in men the testes could be well visualized. The right/left testis ratio was 1.08 ±0.13. Testis/soft tissue and testis/blood activity ratios were highest at 3 h. These ratios were higher than those obtained with pertechnetate at 20 min post injection.99mTc-l<4 appears to be a promising radiopharmaceutical for the scintigraphic visualization of testes.

Effect of Temperature on ADP-Induced Platelet Aggregation

1973 ◽  
Vol 29 (01) ◽  
pp. 183-189
Author(s):  
C. A Praga ◽  
E. M Pogliani
Keyword(s):  
Platelet Aggregation ◽  
Incubation Period ◽  
Room Temperature ◽  
Important Variable ◽  
Practical Significance ◽  
Effect Of Temperature ◽  
Induce Platelet Aggregation ◽  
Cuvette Holder ◽  
Exact Temperature

SummaryTemperature represents a very important variable in ADP-induced platelet aggregation.When low doses of ADP ( < 1 (μM) are used to induce platelet aggregation, the length of the incubation period of PRP in the cuvette holder of the aggregometer, thermostatted at 37° C, is very critical. Samples of the same PRP previously kept at room temperature, were incubated for increasing periods of time in the cuvette of the aggregometer before adding ADP, and a significant decrease of aggregation, proportional to the length of incubation, was observed. Stirring of the PRP during the incubation period made these changes more evident.To measure the exact temperature of the PRP during incubation in the aggre- gometer, a thermocouple device was used. While the temperature of the cuvette holder was stable at 37° C, the PRP temperature itself increased exponentially, taking about ten minutes from the beginning of the incubation to reach the value of 37° C. The above results have a practical significance in the reproducibility of the platelet aggregation test in vitro and acquire particular value when the effect of inhibitors of ADP induced platelet aggregation is studied.Experiments carried out with three anti-aggregating agents (acetyl salicyclic acid, dipyridamole and metergoline) have shown that the incubation conditions which influence both the effect of the drugs on platelets and the ADP breakdown in plasma must be strictly controlled.

Human Platelet Acetylcholinesterase: The Effects of Anticholinesterases on Platelet Function

1979 ◽  
Vol 42 (05) ◽  
pp. 1615-1619 ◽  
Author(s):  
Martin J Smith ◽  
Boyd Braem ◽  
Kent D Davis
Keyword(s):  
Platelet Function ◽  
Ache Activity ◽  
Room Temperature ◽  
Platelet Rich Plasma ◽  
Complete Inhibition ◽  
Clot Retraction ◽  
Plasma Clot ◽  
Normal Platelet ◽  
Aggregation Factor

SummaryPlatelet acetylcholinesterase (AChE) activity was measured in gel-filtered platelet preparations. Three different anticholinesteratic agents (eserine, neostigmine, and diiso- propylphosphorofluoridate) at final concentrations of 10 μM caused complete inhibition of AChE activity after 30 min incubation at room temperature with either platelet-rich plasma or gel-filtered platelets. Complete inhibition of platelet AChE had no effect on platelet aggregation, factor-3 availability, and plasma clot retraction. We conclude that platelet membrane AChE activity is not required for normal platelet function as measured by these in vitro parameters.

PERILAKU DISOLUSI KETOPROFEN DAN INDOMETASIN FARNESIL TERSALUT GEL KITOSAN-GOM GUAR

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Purwantiningsih Sugita ◽  
Bambang Srijanto ◽  
Budi Arifin ◽  
Fithri Amelia ◽  
Mahdi Mubarok
Keyword(s):  
Room Temperature ◽  
Guar Gum ◽  
Tween 80 ◽  
Chitosan Solution ◽  
In Vitro Dissolution ◽  
Dissolution Profile ◽  
Magnetic Stirrer ◽  
Dissolution Behaviour ◽  
Shrimp Shell Waste

Chitosan, a modification of shrimp-shell waste, has been utilized as microcapsule. However, it’s fragile gel property needs to be strengthened by adding glutaraldehyde (glu) and natural hydrocolloid guar gum (gg). This research’s purposes were to study dissolution behaviour of ketoprofen and infar through optimum chitosan-guar gum microcapsule. Into 228.6 mL of 1.75% (w/v) chitosan solution in 1% (v/v) acetic acid,38.1 mL of gg solution was added with concentration variation of 0.35, 0.55, and 0.75% (w/v) for ketoprofen microcapsules and 0.05, 0.19, and 0.33% (w/v) for infar microcapsules, and stirred with magnetic stirrer until homogenous. Afterwards, 7.62mL of glu was added slowly under stirring, with concentrations varied: 3, 3.5, and 4% (v/v) for ketoprofen microcapsules, and 4, 4.5, and 5% (v/v) for infar microcapsules. All mixtures were shaked for 20 minutes for homogenization. All mixtures wereshaked for 20 minutes for homogenization. Into each  microcapsule mixture for ketoprofen, a solution of 2 g of ketoprofen in 250 mL of 96% ethanol was added, whereas solution of 100 mg of in 250 mL of 96% ethanol was added into each microcapsule mixture for infar. Every mixture was then added with 5 mL of 2% Tween-80 and stirred with magnetic stirrer for an hour at room temperature. Everymixture was then added with 5 mL of 2% Tween-80 and stirred with magnetic stirrer for an hour at room temperature. Conversion of suspension into fine powders/granules (microcapsules) was done by using spray dryer. The data of [gg], [glu], and medicine’s content from each microcapsule were treated with Minitab 14 software to obtain optimum [gg] and [glu] for microencapsulation. The dissolution behaviour of optimum ketoprofen and infar microcapsules were investigated. The result of optimization by using Minitab Release 14 software showed that among the microcapsule compositions of [gg] and [glu] were 0.35% (w/v) and 3.75% (v/v), respectively, optimum to coat ketoprofen, whereas [gg] and [glu] of 0.05% (w/v) and4.00% (v/v), respectively, optimum to coat infar, at constant chitosan concentration (1.75% [w/v]). In vitro dissolution profile showed that chitosan-guar gum gel microcapsule was more resistant in intestinal pH condition (rather basic) compared with that in gastric pH (very acidic).

Extract of Polyphenols from Pomegranate Seed and its Antioxidant Activity in Vitro

2020 ◽  
Vol 71 (6) ◽  
pp. 492-499
Author(s):  
Le-Bin Yin ◽  
Dan Liu ◽  
Ai-Lian Yang ◽  
Cong Liao ◽  
Ping He ◽  
...  
Keyword(s):  
Superoxide Anion ◽  
Room Temperature ◽  
Dpph Radical ◽  
Interaction Technique ◽  
Alcohol Extract ◽  
Effective Components ◽  
Pomegranate Seed ◽  
Pomegranate Seeds ◽  
Scavenging Rates

In this study, the pomegranate seeds were treated by micro-cutting assisted interaction technique. The effective components were extracted from pomegranate seeds with 95% ethanol at room temperature, and their antioxidant capacity in vitro was determined. The results showed that the scavenging rates of DPPH radical, superoxide anion radical, hydroxyl radical and lipid peroxidation were 70.97, 51.95, 52.85, and 80.62%, respectively. The antioxidation ability of alcohol extract of pomegranate seed was studied in order to provide theoretical basis for developing more value of pomegranate seed in the future.

Trans-sialidase Associated with Atherosclerosis: Defining the Identity of a Key Enzyme Involved in the Pathology

2019 ◽  
Vol 20 (9) ◽  
pp. 938-941
Author(s):  
Victor Y. Glanz ◽  
Veronika A. Myasoedova ◽  
Andrey V. Grechko ◽  
Alexander N. Orekhov
Keyword(s):  
Acid Metabolism ◽  
Research Subject ◽  
Disease Pathogenesis ◽  
Sialidase Activity ◽  
Ph Values ◽  
Ldl Particles ◽  
Optimum Ph ◽  
Key Enzyme ◽  
St6gal I

Atherosclerosis is associated with the increased trans-sialidase activity, which can be detected in the blood plasma of atherosclerosis patients. The likely involvement in the disease pathogenesis made this activity an interesting research subject and the enzyme that may perform such activity was isolated and characterized in terms of substrate specificity and enzymatic properties. It was found that the enzyme has distinct optimum pH values, and its activity was enhanced by the presence of Ca2+ ions. Most importantly, the enzyme was able to cause atherogenic modification of lowdensity lipoprotein (LDL) particles in vitro. However, the identity of the discovered enzyme remained to be defined. Currently, sialyltransferases, mainly ST6Gal I, are regarded as major contributors to sialic acid metabolism in human blood. In this mini-review, we discuss the possibility that atherosclerosis- associated trans-sialidase does, in fact, belong to the sialyltransferases family.

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