scholarly journals Comparative analysis of the expression of stress-related genes in two pea genotypes contrasting in tolerance to cadmium

2018 ◽  
Vol 16 (4) ◽  
pp. 75-84
Author(s):  
Olga A. Kulaeva ◽  
Emma S. Gribchenko ◽  
Evgeny A. Zorin ◽  
Marina S. Kliukova ◽  
Vladimir A. Zhukov
Keyword(s):  
Comparative Analysis ◽  
Molecular Level ◽  
Toxic Substances ◽  
Expression Of Genes ◽  
Number Of Genes ◽  
Genes Encoding ◽  
Genetic Mechanisms ◽  
Stress Related Genes ◽  
Tolerance To Cadmium ◽  
Initial Line

Background. A major problem of the environmental pollution with heavy metals, including cadmium, requires an intensive study of the molecular and genetic mechanisms underlying the tolerance of plants to these toxic substances. In this study we present a comparative analysis of the expression of stress-related genes in two pea genotypes contrasting in tolerance to cadmium. Materials and methods. A unique mutant of pea SGECdt, characterized by the increased tolerance to cadmium, and initial line SGE were used. Gene expression was analyzed by Real Time PCR. Results. In the line SGE cadmium increase the expression of genes, encoding catalase, chitinase, chitinase-like protein PRP4A and dirigent protein PI206. In the mutant SGECdt cadmium increase the expression of genes, encoding chitinase, glutathione reductase and defensin DRR230. In control samples expression of genes encoding PRP4A and DRRR230 was enhanced in mutant SGECdt versus line SGE. Conclusion. It was shown that, the reaction of the mutant SGECdt at the molecular level differs from that of the line SGE. In the mutant SGECdt, a change in the expression of a number of genes is observed, which may indicate that cadmium entering the cell causes activation of defense reactions.

scholarly journals Biological activity of Nod factors

2020 ◽  
Author(s):  
Dominika Kidaj ◽  
Mikolaj Krysa ◽  
Katarzyna Susniak ◽  
Joanna Matys ◽  
Iwona Komaniecka ◽  
...  
Keyword(s):  
Molecular Level ◽  
Root Nodules ◽  
Cortical Cell ◽  
Plant Tissues ◽  
Nod Factors ◽  
Nod Factor ◽  
Expression Of Genes ◽  
Root Hair Deformation ◽  
Low Concentrations ◽  
Genes Encoding

Chemically, the Nod factors (NFs) are lipochitooligosaccharides, produced mainly by bacteria of the Rhizobium genus. They are the main signaling molecules involved in the initiation of symbiosis between rhizobia and legume plants. Nod factors affect plant tissues at very low concentrations, even as low as 10–12 mol/L. They induce root hair deformation, cortical cell division, and root nodules’ formation in the host plant. At the molecular level, the cytoskeleton is reorganized and expression of genes encoding proteins called nodulins is induced in response to Nod factors in the cell. Action of Nod factors is highly specific because it depends on the structure of a particular Nod factor involved, as well as the plant receptor reacting with it.

scholarly journals Comparative analysis of expression of genes encoding enzymes of catecholamine catabolism and renalase in tissues of normotensive and hypertensive rats

2017 ◽  
Vol 63 (4) ◽  
pp. 312-315 ◽  
Author(s):  
V.I. Fedchenko ◽  
A.E. Medvedev
Keyword(s):  
Comparative Analysis ◽  
Mrna Level ◽  
Mrna Levels ◽  
Hypertensive Rats ◽  
Relative Level ◽  
Mao B ◽  
Wky Rats ◽  
Expression Of Genes ◽  
Mao A ◽  
Genes Encoding

Comparative analysis of expression of genes encoding enzymes of catecholamine catabolism (monoaminbe oxidases A and B (MAO A and MAO B) and catechol-O-methyl transferase (COMT)) and renalase has been carried out in tissues of normotensive Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR). Among investigated tissues the highest level of mRNA of genes encoding key enzymes of catecholamine catabolism (MAO A, MAO B, COMT) was found in the heart of WKY rats. In SHR the mRNA levels of these genes were lower (p<0.05-0.01), however, no similar changes were observed in the tissues studied in dependence of hypertension. The relative mRNA levels of the studied genes normalized versus actin mRNA significantly varied. In heart and kidney the relative level of COMT mRNA significantly exceeded the relative levels of both MAO A mRNA and MAO B mRNA. In the brain differences in mRNAs of MAOA, MAOB, and COMT were less pronounced. However, in all examined tissue the renalase mRNA level was much (at least 10-20-fold) lower than any other mRNA studied. Taking into consideration known correlations between mRNAs and corresponding protein products reported in the literature for many genes these results suggest that in the case of any catalytic scenarios proposed or even proved for renalase this protein cannot contribute to catecholamine degradation. It is also unlikely that the products of renalase reaction, b-NAD(P)+ and hydrogen peroxide, can exhibit a hypotensive effect due to low expression of the renalase encoding gene.

scholarly journals Adaption and Degradation Strategies of Methylotrophic 1,4-Dioxane Degrading Strain Xanthobacter sp. YN2 Revealed by Transcriptome-Scale Analysis

2021 ◽  
Vol 22 (19) ◽  
pp. 10435
Author(s):  
Yingning Wang ◽  
Fang Ma ◽  
Jixian Yang ◽  
Haijuan Guo ◽  
Delin Su ◽  
...  
Keyword(s):  
Degradation Mechanism ◽  
Transcriptional Response ◽  
Malate Synthase ◽  
Expression Of Genes ◽  
Component Systems ◽  
Number Of Genes ◽  
Glyoxylate Metabolism ◽  
Genes Encoding ◽  
Two Component Systems ◽  
Underlying Mechanisms

Biodegradation of 1,4-dioxane (dioxane) contamination has gained much attention for decades. In our previous work, we isolated a highly efficient dioxane degrader, Xanthobacter sp. YN2, but the underlying mechanisms of its extraordinary degradation performance remained unresolved. In this study, we performed a comparative transcriptome analysis of YN2 grown on dioxane and citrate to elucidate its genetic degradation mechanism and investigated the transcriptomes of different dioxane degradation stages (T0, T24, T48). We also analyzed the transcriptional response of YN2 over time during which the carbon source switched from citrate to dioxane. The results indicate that strain YN2 was a methylotroph, which provides YN2 a major advantage as a pollutant degrader. A large number of genes involved in dioxane metabolism were constitutively expressed prior to dioxane exposure. Multiple genes related to the catabolism of each intermediate were upregulated by treatment in response to dioxane. Glyoxylate metabolism was essential during dioxane degradation by YN2, and the key intermediate glyoxylate was metabolized through three routes: glyoxylate carboligase pathway, malate synthase pathway, and anaplerotic ethylmalonyl–CoA pathway. Genes related to quorum sensing and transporters were significantly upregulated during the early stages of degradation (T0, T24) prior to dioxane depletion, while the expression of genes encoding two-component systems was significantly increased at late degradation stages (T48) when total organic carbon in the culture was exhausted. This study is the first to report the participation of genes encoding glyoxalase, as well as methylotrophic genes xoxF and mox, in dioxane metabolism. The present study reveals multiple genetic and transcriptional strategies used by YN2 to rapidly increase biomass during growth on dioxane, achieve high degradation efficiency and tolerance, and adapt to dioxane exposure quickly, which provides useful information regarding the molecular basis for efficient dioxane biodegradation.

scholarly journals Diversity of metabolite accumulation patterns in inner and outer seed coats of pomegranate: exploring their relationship with genetic mechanisms of seed coat development

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Gaihua Qin ◽  
Chunyan Liu ◽  
Jiyu Li ◽  
Yongjie Qi ◽  
Zhenghui Gao ◽  
...  
Keyword(s):  
Seed Coat ◽  
Developmental Stages ◽  
Morphological Differentiation ◽  
Expression Of Genes ◽  
Monolignol Biosynthesis ◽  
Genes Encoding ◽  
Genetic Mechanisms ◽  
Sensory Qualities ◽  
Pomegranate Seeds ◽  
Seed Coats

AbstractThe expanded outer seed coat and the rigid inner seed coat of pomegranate seeds, both affect the sensory qualities of the fruit and its acceptability to consumers. Pomegranate seeds are also an appealing model for the study of seed coat differentiation and development. We conducted nontarget metabolic profiling to detect metabolites that contribute to the morphological differentiation of the seed coats along with transcriptomic profiling to unravel the genetic mechanisms underlying this process. Comparisons of metabolites in the lignin biosynthetic pathway accumulating in seed coat layers at different developmental stages revealed that monolignols, including coniferyl alcohol and sinapyl alcohol, greatly accumulated in inner seed coats and monolignol glucosides greatly accumulated in outer seed coats. Strong expression of genes involved in monolignol biosynthesis and transport might explain the spatial patterns of biosynthesis and accumulation of these metabolites. Hemicellulose constituents and flavonoids in particular accumulated in the inner seed coat, and candidate genes that might be involved in their accumulation were also identified. Genes encoding transcription factors regulating monolignol, cellulose, and hemicellulose metabolism were chosen by coexpression analysis. These results provide insights into metabolic factors influencing seed coat differentiation and a reference for studying seed coat developmental biology and pomegranate genetic improvement.

scholarly journals NtbHLH1, a JAF13-like bHLH, interacts with NtMYB6 to enhance proanthocyanidin accumulation in Chinese Narcissus

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yuxin Fan ◽  
Jiayu Peng ◽  
Jiacheng Wu ◽  
Ping Zhou ◽  
Ruijie He ◽  
...  
Keyword(s):  
Flavonoid Biosynthesis ◽  
Transcriptional Level ◽  
Flavonoid Pathway ◽  
Regulation Of Expression ◽  
Over Expression ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Regulatory Complex ◽  
Positive Regulators ◽  
R2r3 Myb

Abstract Background Flavonoid biosynthesis in plants is primarily regulated at the transcriptional level by transcription factors modulating the expression of genes encoding enzymes in the flavonoid pathway. One of the most studied transcription factor complexes involved in this regulation consists of a MYB, bHLH and WD40. However, in Chinese Narcissus (Narcissus tazetta L. var. chinensis), a popular monocot bulb flower, the regulatory mechanism of flavonoid biosynthesis remains unclear. Results In this work, genes related to the regulatory complex, NtbHLH1 and a R2R3-MYB NtMYB6, were cloned from Chinese Narcissus. Phylogenetic analysis indicated that NtbHLH1 belongs to the JAF13 clade of bHLH IIIf subgroup, while NtMYB6 was highly homologous to positive regulators of proanthocyanidin biosynthesis. Both NtbHLH1 and NtMYB6 have highest expression levels in basal plates of Narcissus, where there is an accumulation of proanthocyanidin. Ectopic over expression of NtbHLH1 in tobacco resulted in an increase in anthocyanin accumulation in flowers, and an up-regulation of expression of the endogenous tobacco bHLH AN1 and flavonoid biosynthesis genes. In contrast, the expression level of LAR gene was significantly increased in NtMYB6-transgenic tobacco. Dual luciferase assays showed that co-infiltration of NtbHLH1 and NtMYB6 significantly activated the promoter of Chinese Narcissus DFR gene. Furthermore, a yeast two-hybrid assay confirmed that NtbHLH1 interacts with NtMYB6. Conclusions Our results suggest that NtbHLH1 may function as a regulatory partner by interacting directly with NtMYB6 to enhance proanthocyanidin accumulation in Chinese Narcissus.

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