scholarly journals The influence of new coumarin derivatives on survival rate of mice in model conditions of acute hypoxia

2020 ◽  
Vol 19 (4) ◽  
pp. 103-108
Author(s):  
Olga M. Rodionova ◽  
Albina F. Safonova ◽  
Anton O. Kashirin ◽  
Valeriy A. Polukeev ◽  
Eugenii R. Bychkov ◽  
...  
Keyword(s):  
Survival Rate ◽  
Sodium Nitrite ◽  
Acute Hypoxia ◽  
Life Time ◽  
Distilled Water ◽  
Coumarin Derivatives ◽  
Test Compound ◽  
Hypoxia With Hypercapnia ◽  
Positive Effect ◽  
Histotoxic Hypoxia

Objective. The article is devoted to study the anti-hypoxemic properties of new coumarin derivatives in the models of hypoxemic hypoxia with hypercapnia, hemic hypoxia and histotoxic hypoxia. Materials and methods. Нypoxemic hypoxia with hypercapnia was modeled as follows: mice were placed in hermetic 200 cm3 jars one in a jar. Hemic hypoxia was reproduced in mice by single subcutaneous introduction of sodium nitrite in a dose of 230 mg/kg. Histotoxic hypoxia was caused in mice by intraperitoneal introduction of sodium nitroprusside in a dose of 20 mg/kg. Coumarin derivatives under lab codes IEM-2266 and IEM-2267 were dissolved in distilled water with addition of twin-80, and then a single intraperitoneal infusion of them in doses 25 and 50 mg/kg was made 45 minutes before placing to the model conditions. Increased life time of an animal compared with the control served the criterion of anti-hypoxemic effect of the studied substances. Results. In hypoxemic hypoxia with hypercapnia test compound under IEM-2267 in doses of 25 and 50 mg/kg increased mice life time by 26 and 34% respectively in comparison with control. In hemic hypoxia model, the positive effect was seen with IEM-2266 compound in a dose of 50 mg/kg which increased life time of animals by 45% in comparison with control. In histotoxic hypoxia model, at preventive introduction of IEM-2266 compound in a dose of 25 mg/kg and IEM-2267 in a dose of 50 mg/kg life time increased up to 117% and 123% respectively. Conclusion. The coumarin derivatives IEM-2266 and IEM-2267 relieved the course of acute hypoxia and increased life time of animals in the models of hypoxemic hypoxia with hypercapnia, hemic hypoxia and histotoxic hypoxia.

Evaluation of the activity of antihypoxants with an isothiourea structure in a model of hypercapnic hypoxia with a shutdown of the cerebral hemispheres

2021 ◽  
Vol 19 (1) ◽  
pp. 55-63
Author(s):  
Vera V. Marysheva ◽  
Vladimir V. Mikheev ◽  
Petr D. Shabanov
Keyword(s):  
Acute Hypoxia ◽  
Life Time ◽  
The Body ◽  
Cerebral Hemispheres ◽  
Antihypoxic Activity ◽  
Hypoxic Episode ◽  
Hypoxia With Hypercapnia ◽  
Hypercapnic Hypoxia ◽  
Outbred Mice ◽  
The Brain

PURPOSE: To study the effect of amtizol, 2-aminobenzthiazole (2-ABT) and 2-amino-4-acetylthiazolo[5,4-b]indole (BM-606) on the resistance of male outbred mice to acute hypoxia with hypercapnia under conditions of isolated functioning of one from the hemispheres, as well as both hemispheres of the brain. METHODS: A model of acute hypoxia with hypercapnia (canned hypoxia) was used in mice of the same mass, the lifespan of all animals was determined. Temporary shutdown of the cortex of one of the hemispheres or both hemispheres was achieved by epidural application of filter paper moistened with 25% potassium chloride solution, creating a spreading depression according to Leao. Amtizol, 2-aminobenzthiazole (2-ABT) and 2-amino-4-acetylthiazolo[5,4-b]indole (BM-606) at equimolar doses of 25, 32.5, and 50 mg/kg, respectively were used as pharmacological analyzers, the compounds were injected intraperitoneally 30 min before the hypoxic episode. RESULTS: It was shown that, in contrast to amtizol, 2-ABT and VM-606 increase the life time of experimental animals when any hemisphere is turned off. The use of drugs when both hemispheres were turned off revealed that amtizol has approximately equal effect on the brain and the rest of the body, in 2-ABT antihypoxic activity is 1/3 associated with the brain, in VM-606 exclusively with the brain. CONCLUSION: The experimental model used in this work makes it possible to quite easily evaluate the effect of either one drug or compare several drugs, their role in the functioning of the cerebral hemispheres, on which part of the sample highly resistant or low resistant to hypoxia they have the greatest effect.

EFFECT OF AMINOTHIOL ANTIHyPOXANTS ON INTERHEMISPHERIC ASyMMETRy OF THE bRAIN

2012 ◽  
Vol 10 (1) ◽  
pp. 51-53
Author(s):  
Vladimir Vladimirovich Mihkeev ◽  
Vera Vasilievna Marysheva ◽  
Boris Nikolaevich Bogomolov ◽  
Lubov Vladislavovna Zhukova-Williams
Keyword(s):  
Left Hemisphere ◽  
Right Hemisphere ◽  
Acute Hypoxia ◽  
Life Time ◽  
Antihypoxic Activity ◽  
Interhemispheric Relations ◽  
Hypoxia With Hypercapnia ◽  
Antihypoxic Effect ◽  
The Right ◽  
The Brain

The effect of aminothiol antihipoxants amthizol and its analogue VM-606 on the resistance of the SHR mice males to an acute hypoxia with hypercapnia under conditions of isolated functioning of one of the hemispheres of the brain was studied. Antihypoxic agent amthizol 25 mg/kg increases life time of naïve mice by 46.2%. The drug acted on the sham-operated mice more slightly, increasing of their life only on 28.1% (p<0.01). Administration of amthizol under conditions of functioning of the right hemisphere significantly enhanced (+64.8%) the life time of mice. No antihypoxic effect was registered after administration of amthizol to mice with active left hemisphere: the result was the same as in mice without amthizol. Therefore, antihypoxic effect of amthizol was due to its action on the right (but not the left) hemisphere of the brain. VM-606 possessed more antihypoxic activity in comparison with amthizol. After unilateral cortical inactivation, VM-606 increased life time of mice both in active right and active left hemispheres, but in more degree in active right hemisphere. Thus, interhemispheric differences in resistance of mice to hypoxia with hypercapnia were diminished. Therefore, the differences between amthizol and VM-606 are the followings: amthizol inverts interhemispheric relations in hypoxia whereas VM-606 diminishes them.

The Time-Course of Changes in the State of Brain Monoaminergic Systems of Mice Under the Acute Hypoxia with Hypercapnia

2020 ◽  
Vol 14 (2) ◽  
pp. 136-149
Author(s):  
I. V. Karpova ◽  
V. V. Mikheev ◽  
V. V. Marysheva ◽  
N. A. Kuritcyna ◽  
E. R. Bychkov ◽  
...  
Keyword(s):  
Time Course ◽  
Acute Hypoxia ◽  
The State ◽  
Hypoxia With Hypercapnia

scholarly journals Prolonging the vase life of cut Carnation 'GIOKO' by using different chemicals

2008 ◽  
Vol 14 (4) ◽  
Author(s):  
O. Terék ◽  
F. A. S. Hassan ◽  
E. Jámbor-Benczúr ◽  
Á. Máthé
Keyword(s):  
Untreated Control ◽  
Dianthus Caryophyllus ◽  
Vase Life ◽  
Distilled Water ◽  
Cut Flowers ◽  
Chemical Treatments ◽  
All Solutions ◽  
Separate Treatment ◽  
Flower Diameter ◽  
Positive Effect

Cut flowers of Dianthus caryophyllus L. cv. GIOKO were treated with different concentrations of sucrose and in combination with 1­methylcyclopropene (1-MCP) to compare the effect of these treatments with floral preservative (`Spring') on the longevity of flowers. Distilled water was used for preparing all solutions. The control flowers were held in distilled water. Clorox at 2 mL- I was added to all treatments containing sucrose and it was also applied as a separate treatment. The vase life of cut carnations was significantly prolonged due to the use of chemical treatments, as compared to the untreated control. The longest vase life (18.33 days) was obtained by using 1-MCP 0.5 g m-3 for 6 h treatment. All concentrations of sucrose had a positive effect on flower diameter. The best treatment in this respect was 1 -MCP with 30 gL-I sucrose. 1-MCP treatment significantly increased the chlorophyll content, as compared to the control or the "Spring" treatment. The highest values in this respect were obtained by 1 -MCP treatment alone or with the lowest level of sucrose. The effect of these treatments on the pH of solutions is discussed.

THE EFFECT OF NORADRENALINE ON THE SURVIVAL OF RATS SUBJECTED TO HEMORRHAGIC SHOCK

1957 ◽  
Vol 35 (1) ◽  
pp. 93-101 ◽  
Author(s):  
A. M. Lansing ◽  
J. A. F. Stevenson ◽  
C. W. Gowdey
Keyword(s):  
Survival Rate ◽  
Hemorrhagic Shock ◽  
Survival Time ◽  
Intravenous Infusion ◽  
Survival Rates ◽  
Distilled Water ◽  
Sprague Dawley ◽  
Chi Square ◽  
Sprague Dawley Rats ◽  
Chi Square Test

Reports of the efficacy of l-noradrenaline in the treatment of clinical shock stimulated an investigation of its effect in controlled hemorrhagic hypotension. Seventy-three 350-g. male Sprague–Dawley rats were subjected to a standardized hemorrhagic shock procedure. Of 15 control animals that received no treatment, only one survived for 48 hours; none survived of the five controls that received a constant intravenous infusion, after the shock procedure, of 5% glucose in distilled water until death or for 36 hours. The treated animals received, after the shock procedure, an infusion of l-noradrenaline (0.5–2.0 μg./min.) in 5% glucose in distilled water. The survival rates for the treated animals were: treatment for 1 hour, 1/8; treatment for 4 hours, 4/15; treatment until death or for 36 hours, 8/15. Fifteen animals received, in addition to noradrenaline for 36 hours, hydrocortisone administered intravenously (0.7 μg./min.) or intramuscularly (2.5 mg. every 6 hours); seven of these animals survived.Analysis of variance showed that there was no difference in the shock procedure undergone by the controls and by the treated survivors. The Chi square test on the survival rates revealed that the infusion of noradrenaline for 1 hour or 4 hours did not improve survival, but infusion for 36 hours produced a very significant increase in survival time and in total survival rate. The addition of hydrocortisone neither enhanced nor impaired this improvement.

scholarly journals Growth response of Alnus viridis to application of crushed limestone and amphibolite and forestry potential of the species in harsh acidic mountain sites

2011 ◽  
Vol 57 (No. 5) ◽  
pp. 200-209 ◽  
Author(s):  
I. Kuneš ◽  
T. Koňasová ◽  
V. Balcar ◽  
M. Baláš ◽  
D. Zahradník ◽  
...  
Keyword(s):  
Survival Rate ◽  
Initial Response ◽  
Growth Potential ◽  
Good Growth ◽  
Acidic Site ◽  
Growing Seasons ◽  
Alnus Viridis ◽  
Harsh Conditions ◽  
Positive Effect ◽  
Crushed Limestone

An experiment with seedlings of Alnus viridis was established on a clear-felled tract situated on a summit tableland of the Jizersk&eacute; hory Mts. (central Europe, Czech Republic) at an altitude of 980 m a.s.l. The aims of the experiment were (i) to evaluate the response of A. viridis to the application of a basic mixture containing crushed limestone and amphibolite and (ii) to assess the growth potential of the species in a poor acidic site under climatically harsh conditions. Alnus viridis showed a good growth performance and survival rate, its initial response to fertilization was positive. The height growth and expansion of crowns were slightly but significantly stimulated by the treatment, the survival rate was also increased, though not significantly. The amendment application temporarily increased the foliar Ca and Mg concentrations. The content of foliar P, of the most deficient macroelement, was not improved by the treatment. However, the positive effect of fertilization was short-lasting and unconvincing from the aspect of practical use. In the second half of the reference period the control started to gain on the fertilized variant and as for mean height and crown diameter, both variants got almost equalized eight growing seasons after the treatment.

Acute hypoxia influence on cardio-respiratory system and new possibilities of pharmacological prophylactics of hypoxia in experiment

2016 ◽  
Vol 14 (1) ◽  
pp. 37-45
Author(s):  
Andrei V Evseev ◽  
Vitaliy A Pravdivtsev ◽  
Denis V Sosin ◽  
Marina A Yevseyeva
Keyword(s):  
Metal Complex ◽  
Electrical Activity ◽  
Respiratory System ◽  
High Resistance ◽  
Acute Hypoxia ◽  
Cardiorespiratory System ◽  
Resistance Level ◽  
Hypoxia With Hypercapnia ◽  
Hypercapnic Hypoxia ◽  
Complex Substance

Cardiorespiratory system activities have been studied on rats in experiment after oral introduction of the new selenium-containing metal-complex substance πQ1983 in dose 100 mg/kg before and under action of acute hypercapnic hypoxia (AH + Hc). The substance was introduced 90 min. before (incubation period) placement of animals into hypoxic chambers with 1.0 L free volume. During each experiment as well as during AH+Hc an electrical activity of myocardium (ECG) and respiration curves called pneumobarogramm (PBG) were recorded simultaneously. It has been established that the substance πQ1983 made cardiodepressive effect and decreases parameters of lungs ventilation in animals. According to ECG and PBG dynamics, rats protected by the substance demonstrated a high resistance level to the aroused acute hypoxia with hypercapnia, that expressed by weakening of early reactions from cardiorespiratory system under hypercapnia action, and by twice longer life span of animals in hypoxic experiment.

Central monoaminergic systems sensitivity to acute hypoxia with hypercapnia changes after the maintenance under the long-term social isolation

2018 ◽  
Vol 64 (6) ◽  
pp. 511-516 ◽  
Author(s):  
I.V. Karpova ◽  
V.V. Mikheev ◽  
V.V. Marysheva ◽  
N.A. Kuritcyna ◽  
E.R. Bychkov ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Social Isolation ◽  
Acute Hypoxia ◽  
Brain Structures ◽  
Hypoxia With Hypercapnia ◽  
Long Time ◽  
The Right ◽  
The Brain ◽  
Monoamines And Their Metabolites

The experiments were performed in male albino outbred mice kept in a group and under the conditions of long-term social isolation. The changes in the monoaminergic systems of the left and right hemispheres of the brain after acute hypoxia with hypercapnia have been studied. The levels of dopamine (DA), serotonin (5-HT) and their metabolites – dioxyphenylacetic (DOPAC), homovanillic (HVA), and 5-hydroxyindoleacetic (5-HIAA) acids – were determined by HPLC in the cerebral cortex, hippocampus and striatum of the right and left sides of the brain. In the control mice kept both in the group and under the conditions of social isolation, a higher content of DA in the cortex of the left hemisphere has been found. In the other brain structures the monoamine content was symmetric. In the cerebral cortex of the mice in the group, acute hypoxia with hypercapnia led to a right-sided increase in the DA and 5HT levels. At the same time, the DOPAC content decreased in the left cortex. In mice in the group, under the hypoxia with hypercapnia conditions, the DA level in the left hippocampus increased. In the striatum, the content of monoamines and their metabolites did not change significantly. In animals kept for a long time under the conditions of social isolation, hypoxia with hypercapnia no statistically significant changes in the monoamines and their metabolites levels were found. It has been concluded that the preliminary maintenance under the conditions of prolonged social isolation changes the reaction of central monoaminergic systems to acute hypoxia with hypercapnia.

EFFECTS OF NALOXONE AND MORPHINE ON SURVIVAL RATE OF MICE AGAINST ACUTE HYPOXIA

1986 ◽  
Vol 65 (Supplement 3A) ◽  
pp. A463 ◽  
Author(s):  
H. Endoh ◽  
K. Shimoji ◽  
E. Naito ◽  
Y. Satoh
Keyword(s):  
Survival Rate ◽  
Acute Hypoxia

scholarly journals A method to increase the survival of probiotic bacteria Lactobacillus brevis at a lowered pH

2019 ◽  
Vol 54 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Andrzej Jurkowski ◽  
Jacek J. Kozioł ◽  
Ewelina Gronczewska
Keyword(s):  
Lactic Acid ◽  
Survival Rate ◽  
Magnetic Nanoparticles ◽  
Lactic Acid Production ◽  
Lactobacillus Brevis ◽  
Optimum Concentration ◽  
Probiotic Bacteria ◽  
Acid Fermentation ◽  
Minimum Concentration ◽  
Positive Effect

Abstract Lactobacillus brevis PCM 2570 is a strain of lactic acid bacteria, i.e. probiotic bacteria whose major fermentation product is lactic acid. The efficiency of lactic acid production is limited by the value of ambient pH. This study aimed to increase the survival of this bacterial strain at a reduced pH (3.9), which would result in an increased yield of lactic acid fermentation. In our experiment the survival rate of probiotic bacteria L. brevis PCM 2570 was increased 1.2-fold to 6.96-fold due to the presence of Fe3O4 magnetic nanoparticles, as compared to the control. The minimum concentration of nanoparticles with a positive effect was 8 mg/ml, but the optimum concentration was 20 mg/ml.

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