Antimicrobial Activity of Kluwek Seed Extract as Natural Preservatives of Tuna Fish Ball
Kluwek seeds contain antimicrobial compounds that can be categorized as preservative agents. The<br />main objective of this study was to evaluate the effect of the addition of polar extract of kluwek seeds against<br />microbiological quality and sensory quality and shelf life of tuna fish balls. This study used a factorial<br />completely randomized design with various extract concentrations and storage duration treatments, each<br />of which conducted in three replications. The variables measured were total microbial and organoleptic<br />acceptance. In this study, the fishballs were immersed in 2%, 4%, 6% and 8% b/v extract for 60 minutes,<br />then stored for three days at room temperature (28-30ºC). The results showed that the the highest inhibitory<br />activity against microbial growth was exhibited by meatballs immersed in 8% concentration, in which total<br />microbial load up to the third day storage was 1,32 x 102 CFU/g which was still under INS quality for fish<br />balls of 1 x 107. Fishballs from 0 day storage was more accepted by panelists compared to fishballs from 1,<br />2, and 3 days storage in terms of organoleptic acceptability and fishballs were still accepted organoleptically<br />only until one day storage, while fishballs stored for 2 and 3 days have deteriorated based on visual<br />observation of the color, odor, taste and texture.<br /><br />