scholarly journals Foxtail Millet Stress Associated Protein Gene SiSAP4 Enhances Drought Stress Tolerance in Transgenic Arabidopsis

2021 ◽  
Vol 25 (02) ◽  
pp. 441-449
Author(s):  
Wenlu Li
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Stress Tolerance ◽  
Transcriptional Activation ◽  
Transgenic Arabidopsis ◽  
Expression Patterns ◽  
Foxtail Millet ◽  
Expression Level ◽  
Drought Stress Tolerance ◽  
Transgenic Breeding

Abiotic stresses like drought affect plant growth and crop yield with climate change worsening. Stress associated proteins (SAPs), as the zinc finger proteins with A20/AN1 domain, play an important role in regulating abiotic stress response. As a typical summer dryland grain crop in the north of China, foxtail millet has the characteristics of drought resistance, making it a valuable resource for anti-stress gene exploitation and utilization. In this study, SiSAP4 gene was cloned from foxtail millet variety Yugu 1. Analysis showed that SiSAP4 gene was expressed in roots, stems and leaves at seedling stage, and the highest expression level was detected in leaves. Expression patterns under different stress conditions showed that expression level of SiSAP4 gene was significantly up-regulated under drought stress, suggesting it may be involved in drought stress response. Subcellular localization indicated that SiSAP4 was present in the nucleus and cytoplasm. It was revealed that SiSAP4 had no function in transcriptional activation in the yeast system. Overexpression of SiSAP4 in transgenic Arabidopsis resulted in enhanced tolerance to drought stress, which was simultaneously demonstrated by increased expression of a broad range of stress response genes. Based on those results, SiSAP4 has the potential to be used in transgenic breeding to improve drought stress tolerance in other crops. © 2021 Friends Science Publishers

scholarly journals Overexpression of the ThTPS gene enhanced T. hispida salt and drought stress tolerance

2020 ◽  
Author(s):  
PeiLong Wang ◽  
XiaoJin Lei ◽  
JiaXin Lv ◽  
caiqiu gao
Keyword(s):  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Expression Patterns ◽  
Evolutionary Relationship ◽  
Drought Stress Tolerance ◽  
Trehalose Metabolism ◽  
Physiological Indexes ◽  
Salt And Drought Stress

Abstract Background: Trehalose is a nonreducing disaccharide with high stability and strong water absorption properties that can improve the resistance of organisms to various abiotic stresses. Trehalose-6-phosphate synthase (TPS) plays important roles in trehalose metabolism and signaling. Results: A full-length cDNA of ThTPS was cloned from Tamarix hispida. The phylogenetic tree among ThTPS and 11 AtTPS in Arabidopsis indicates that the ThTPS protein had a close evolutionary relationship with AtTPS7. However, the function of AtTPS7 has not been determined. To analyze the abiotic stress tolerance function of ThTPS, the expression patterns of ThTPS were monitored under salt and drought stress and JA, ABA and GA3 hormone stimulation in T. hispida by qRT-PCR. The results showed that ThTPS expression was clearly induced by these 5 kinds of treatments at at least one studied point. Particularly under salt stress, ThTPS was highly induced in the roots of T. hispda. Furthermore, the ThTPS gene was transiently overexpressed in T. hispida. The results of physiological indexes and staining showed that overexpression of the ThTPS gene increased T. hispida salt and drought stress tolerance. Conclusion: The ThTPS gene can respond to abiotic stress such as salt and drought, and overexpression of ThTPS gene can significantly improve salt and drought tolerance. These findings establish a foundation to better understand the response of TPS genes to abiotic stress in plants.

scholarly journals Genome-Wide Analysis of MADS-Box Genes in Foxtail Millet (Setaria italica L.) and Functional Assessment of the Role of SiMADS51 in the Drought Stress Response

2021 ◽  
Vol 12 ◽  
Author(s):  
Wan Zhao ◽  
Li-Li Zhang ◽  
Zhao-Shi Xu ◽  
Liang Fu ◽  
Hong-Xi Pang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Tolerance ◽  
Abiotic Stresses ◽  
Survival Rates ◽  
Foxtail Millet ◽  
Negative Regulator ◽  
Setaria Italica ◽  
Mads Box ◽  
Drought Stress Tolerance ◽  
Genome Wide

MADS-box transcription factors play vital roles in multiple biological processes in plants. At present, a comprehensive investigation into the genome-wide identification and classification of MADS-box genes in foxtail millet (Setaria italica L.) has not been reported. In this study, we identified 72 MADS-box genes in the foxtail millet genome and give an overview of the phylogeny, chromosomal location, gene structures, and potential functions of the proteins encoded by these genes. We also found that the expression of 10 MIKC-type MADS-box genes was induced by abiotic stresses (PEG-6000 and NaCl) and exogenous hormones (ABA and GA), which suggests that these genes may play important regulatory roles in response to different stresses. Further studies showed that transgenic Arabidopsis and rice (Oryza sativa L.) plants overexpressing SiMADS51 had reduced drought stress tolerance as revealed by lower survival rates and poorer growth performance under drought stress conditions, which demonstrated that SiMADS51 is a negative regulator of drought stress tolerance in plants. Moreover, expression of some stress-related genes were down-regulated in the SiMADS51-overexpressing plants. The results of our study provide an overall picture of the MADS-box gene family in foxtail millet and establish a foundation for further research on the mechanisms of action of MADS-box proteins with respect to abiotic stresses.

scholarly journals A wheat R2R3-MYB gene, TaMYB30-B, improves drought stress tolerance in transgenic Arabidopsis

2012 ◽  
Vol 63 (16) ◽  
pp. 5873-5885 ◽  
Author(s):  
Lichao Zhang ◽  
Guangyao Zhao ◽  
Chuan Xia ◽  
Jizeng Jia ◽  
Xu Liu ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Tolerance ◽  
Transgenic Arabidopsis ◽  
Drought Stress Tolerance ◽  
Myb Gene ◽  
R2r3 Myb

The grape VvMBF1 gene improves drought stress tolerance in transgenic Arabidopsis thaliana

2014 ◽  
Vol 118 (3) ◽  
pp. 571-582 ◽  
Author(s):  
Qin Yan ◽  
Hongmin Hou ◽  
Stacy D. Singer ◽  
Xiaoxiao Yan ◽  
Rongrong Guo ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Transgenic Arabidopsis ◽  
Drought Stress Tolerance ◽  
Transgenic Arabidopsis Thaliana

scholarly journals Genome-Wide Analysis of the MADS-Box Gene Family in Foxtail Millet (Setaria Italica L.) and Expression Analyses to Reveal Their Role During Drought Stress

2021 ◽  
Author(s):  
Xinlei Ma ◽  
Ningwei XU ◽  
Pengpeng Gu ◽  
Liqiang Du ◽  
Zhenqing Guo ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Gene Family ◽  
Expression Patterns ◽  
Foxtail Millet ◽  
Setaria Italica ◽  
Pcr Analysis ◽  
Induced Response ◽  
Mads Box ◽  
Element Analysis

Abstract MADS-box gene family is a key regulatory factor family, which controls vegetative growth, reproductive development and can be used to mediate abiotic stresses in many plants. However, Knowledge of this gene family is still limited in Setaria italica. In the present study, a total of 70 SitMADS genes were identified and renamed on the basis of the chromosomal distribution of the SitMADS genes. According to gene structure, conserved motif and phylogenetic feature, the 70 SitMADSs were classified into type-Ⅰ (Mα, Mβ, Mγ) and type-Ⅱ (MIKCC and MIKC*). All of the SitMADS genes were randomly distributed on nine chromosomes, and five tandem duplicated genes and 12 pairs of duplicated gene segments were detected in the SitMADS genes family. Synteny analysis provided a high homology between SitMADS genes and OsMADS genes. A cis-element analysis inferred that SitMADS genes, except for SitMADS23, possessed at least one drought stress response and ABA(Abscisic Acid)-induced response cis-element. Real-time quantitative PCR analysis was used to detect the expression patterns of SitMADS genes in various tissues and demonstrated that the genes responded drought stress and ABA treatments. SitMADS23, SitMADS42, SitMADS51, SitMADS52, SitMADS58 and SitMADS64 were highly expressed in PEG(Polyethylene glycol) and drought stress, which suggested its important role in drought stress response. SitMADS51, SitMADS63 and SitMADS64 seemed to be responsive to ABA hormone signaling, suggesting that they were involved in the ABA signaling pathways. This paper provided a deep insight into the evolutionary characteristics of SitMADS genes. The results provide comprehensive information for further analyses of the molecular functions of the MADS-box gene family in Setaria italica.

scholarly journals Multi-Omics-Based Identification and Functional Characterization of Gh_A06G1257 Proves Its Potential Role in Drought Stress Tolerance in Gossypium hirsutum

2021 ◽  
Vol 12 ◽  
Author(s):  
Teame Gereziher Mehari ◽  
Yanchao Xu ◽  
Muhammad Jawad Umer ◽  
Margaret Linyerera Shiraku ◽  
Yuqing Hou ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Tolerance ◽  
Gene Networks ◽  
Potential Role ◽  
Expression Patterns ◽  
Functional Characterization ◽  
Hub Genes ◽  
Drought Stress Tolerance ◽  
Gene Modules

Cotton is one of the most important fiber crops globally. Despite this, various abiotic stresses, including drought, cause yield losses. We used transcriptome profiles to investigate the co-expression patterns of gene networks associated with drought stress tolerance. We identified three gene modules containing 3,567 genes highly associated with drought stress tolerance. Within these modules, we identified 13 hub genes based on intramodular significance, for further validation. The yellow module has five hub genes (Gh_A07G0563, Gh_D05G0221, Gh_A05G3716, Gh_D12G1438, and Gh_D05G0697), the brown module contains three hub genes belonging to the aldehyde dehydrogenase (ALDH) gene family (Gh_A06G1257, Gh_A06G1256, and Gh_D06G1578), and the pink module has five hub genes (Gh_A02G1616, Gh_D12G2599, Gh_D07G2232, Gh_A02G0527, and Gh_D07G0629). Based on RT-qPCR results, the Gh_A06G1257 gene has the highest expression under drought stress in different plant tissues and it might be the true candidate gene linked to drought stress tolerance in cotton. Silencing of Gh_A06G1257 in cotton leaves conferred significant sensitivity in response to drought stress treatments. Overexpression of Gh_A06G1257 in Arabidopsis also confirms its role in drought stress tolerance. L-valine, Glutaric acid, L-proline, L-Glutamic acid, and L-Tryptophan were found to be the most significant metabolites playing roles in drought stress tolerance. These findings add significantly to existing knowledge of drought stress tolerance mechanisms in cotton.

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