scholarly journals Seed Invigoration with Paclobutrazol Improves Seedling Growth, Physiological, Biochemical Attributes and Fruit Yield in Okra

2021 ◽  
Vol 26 (02) ◽  
pp. 277-286
Author(s):  
Rohina Bashir
Keyword(s):  
Plant Height ◽  
Seedling Growth ◽  
Plant Biomass ◽  
Abiotic Factors ◽  
Germination Rate ◽  
Seed Priming ◽  
Seed Soaking ◽  
Randomized Design ◽  
Effect On Yield ◽  
Amino Acid Contents

The production of okra is affected by improper seedling growth and abiotic factors like temperature and soil. This study was aimed to examine the effect of paclobutrazol (PBZ) seed priming on the growth, seedling vigor and yield of okra. A pot experiment was conducted in completely randomized design under natural conditions and seeds of two okra varieties were soaked in 0, 4, 8, 10 and 20 mg L-1 of PBZ solution. Results showed that PBZ seed soaking affected the germination rate and maximum reduction in germination rate was observed at 20 mg L-1 of PBZ. A gradual decrease in plant height was recorded in PBZ treated plants as compared to control. A decrease in plant height of PBZ treated plants was accompanied with an increase in plant biomass and number of branches. PBZ seed soaking resulted in darker green leaves with higher chlorophyll, protein and total free amino acid contents than control plants. The activity of antioxidant enzymes, peroxidase and superoxide dismutase was also higher in PBZ treated plants. The improvement in all vegetative and physiological parameters ultimately had a positive effect on yield. PBZ treated plants showed an increase in the number of pods and pod length while seed weight remained unaffected. Thus, seed priming with optimum doses of PBZ could improve the seedlings and yield of okra. © 2021 Friends Science Publishers

scholarly journals Efficacy of KNO3, SiO2 and SA priming for improving emergence, seedling growth and antioxidant enzymes of rice (Oryza sativa), under drought

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lawan Gana Ali ◽  
Rosimah Nulit ◽  
Mohd Hafiz Ibrahim ◽  
Christina Yong Seok Yien
Keyword(s):  
Salicylic Acid ◽  
Seedling Growth ◽  
Antioxidant Activities ◽  
Soluble Sugar ◽  
Seed Priming ◽  
Potassium Nitrate ◽  
Total Soluble Protein ◽  
Rice Seedlings ◽  
Chlorophyll Contents ◽  
Randomized Design

AbstractRice is an important staple crop produced and consumed worldwide. However, poor seed emergence is one of the main impediments to obtaining higher yield of rice especially in hot and dry ecosystems of the world that are ravaged by drought. Therefore, this study was carried out to evaluate the effects of potassium nitrate (KNO3), salicylic acid (SA) and silicon dioxide (SiO2) priming in improving emergence, seedling growth, biochemical attributes and antioxidant activities of FARO44 rice under drought conditions. Rice seedlings primed with 2.5% and 5% KNO3, 3% and 3.5% SiO2, and 1 mM and 2.5 mM SA were subjected to three drought levels of low, moderate and severe under the greenhouse. Seed emergence, seedling growth, biochemical attributes and antioxidant activities were thereafter evaluated. Seed priming experiments were laid in a completely randomized design with five replicates per treatment. The results found that rice seedlings responded differently to different priming treatments. However, all primed rice seedlings had significantly (P ≤ 0.05) improved emergence percentage (72–92%), seedling growth, seedling vigor, seedling fresh and dry biomass and shorter emergence time compared with controls. Likewise, total soluble protein content, activities of catalase, ascorbate peroxidase and superoxide dismutase, carbohydrate, soluble sugar and total chlorophyll contents of rice seedlings were increased by more than two-folds by seed priming compared with control. Salicylic acid showed less effect in increasing emergence, seedling growth, antioxidant activities and biochemical attributes of rice. Thus, this study established that seed priming with KNO3 (2.5% and 5%) and SiO2 (3% and 3.5%) were more effective in improving emergence, seedling growth, biochemical attributes and antioxidant activities of FARO44. Thus, priming of FARO44 rice with this chemical is recommended for fast emergence, seedling growth and drought resistance in dry ecosystems.

The Effects of Salinity Stress on Seed Reserve Utilization and Germination Percentage of Treated Seeds of Barley (Hordeum Vulgare L.)

2014 ◽  
Vol 47 (1) ◽  
pp. 23-29
Author(s):  
S.A. Tabatabaei
Keyword(s):  
Hordeum Vulgare ◽  
Salinity Stress ◽  
Seedling Growth ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Utilization Efficiency ◽  
Hordeum Vulgare L ◽  
Randomized Design ◽  
Percentage Weight

ABSTRACT In order to investigate salinity stress on seed reserve utilization and seedling growth of treated seeds of barley (Hordeum vulgare L.), an experiment was carried out. Factorial experiment was carried out in completely randomized design with three replicates. To create salinity stress, NaCl in osmotic levels at 0 (as control), -4, -8, -12 and -16 bar were used. For seed priming, gibberellin (GA) 50 ppm was used. Our results showed that treatment × drought interaction on these traits: germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were significant. The highest germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were attained from priming by gibberellin at control conditions. Thus, priming increased characteristics as compared to the unprimed. Priming improved seed reserve utilization such as: weight of utilized (mobilized) seed reserve, seed reserve depletion percentage, seed reserve utilization efficiency and seedling growth in barley under salinity stress.

scholarly journals Priming Methods: Alternative Strategy to Improve Seed and Seedling Performance of Soursop (Annona muricata)

2019 ◽  
pp. 1-7
Author(s):  
D. J. A. Najorda ◽  
R. J. G. Rosales
Keyword(s):  
Germination Rate ◽  
Seed Priming ◽  
Seed Vigor ◽  
Seedling Performance ◽  
Randomized Design ◽  
Breaking Dormancy ◽  
Polyethylene Bags ◽  
Hormonal Priming ◽  
Completely Randomized Design ◽  
Experimental Treatments

The evaluation of seed priming methods on the seed and seedling performance of soursop was conducted January 9 to April 3, 2019 in San Nicolas, Ilocos Norte, Philippines.  The study was conducted to investigate the effect of seed priming methods  capable of breaking dormancy; improve germination rate; determine the seed vigor of soursop; and identify the best seed priming method that provide better seedling performance. The experimental  treatments (unprimed and three priming methods, hydropriming, halopriming and hormonal priming) were laid out in Completely Randomized Design with three replications. A     total of 20 polyethylene bags were used per treatment per replication with one seed sown in every bag. Alternative way to improve seed and seedling performance is the use of these seed priming methods. The seed and seedling performance of soursop were significantly affected by priming methods. Primed seeds had higher percentage germination rate (PGR) than unprimed seeds. But numerically, the highest PGR was hydropriming. Hormonal priming produced significantly taller seedlings at 10 and 40 days after emergence (DAE) than unprimed seeds and more leaves per seedling at 30 to 50 DAE. Hormonal, hydropriming and halopriming produced significantly higher fresh weight of seedlings than unprimed seeds.

scholarly journals ​Influence of Temperature and Salinity Stress on Seed Germination and Seedling Growth of Soybean (Glycine max L.)

2021 ◽  
Author(s):  
Mustafa Çirka ◽  
Ali Rahmi Kaya ◽  
Tamer Eryiğit
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Germination Rate ◽  
Petri Dish ◽  
Negative Effects ◽  
Randomized Design ◽  
Salt Level ◽  
Glycine Max L ◽  
Early Seedling ◽  
Lower Temperature

Background: The high salt level of a germinating environment can lead to reduced, delayed, and even complete inhibition of germination and seeding growth due to osmotic action and/or ion toxicity. Based on this viewpoint, the aim of this study was to investigate germination temperature and salinity effects on germination and early seedling growth of soybean, which can be sown as first and second crops. Methods: Soybean seeds were subjected to NaCl induced saline germinating media prepared in petri dishes under two different germination temperatures (20±2 and 25±2oC). Thirty sterilized seeds per petri dish were sown in ten salt treatments (0, 100, 150, 250, 750, 1000, 2250, 5000, 7500 and 10000 ppm NaCl L-1). The study was carried out according to the completely randomized design with four replications. Result: Lower temperature promoted seed germination, while the high temperature significantly inhibited the seed germination at all NaCl doses tested. As a result, 25oC temperatures, which can only be measured at the time of the second sowing, have been found to negatively affect germination and also increase the negative effects of salt. Due to the moderate tolerance of soybeans to salt stress, the germination rate was positively affected up to 750 ppm NaCl L-1 dose and resulted in severe reductions in subsequent doses. Also, the tolerance of soybean was negatively influenced by the interaction of temperature and NaCl concentration.

scholarly journals Effect of seed priming with kinetin on germination indices and proline activity of Silybum marianum L. under drought stress conditions

2015 ◽  
Vol 9 (1) ◽  
pp. 6-11
Author(s):  
Abolfazl Masoumi Zavariyan ◽  
Mojtaba Yousefi Rad ◽  
Mohsen Asghari
Keyword(s):  
Drought Stress ◽  
Germination Rate ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Silybum Marianum ◽  
Randomized Design ◽  
Seedling Length ◽  
Germination Indices

For investigation of the effect of seed priming by kinetin on germination indices and proline activity of Silybum marianum  L. under drought stress, factorial experiment based on completely randomized design with two factor includingdifferent concentrations of kinetin (0, 10 and 20 ppm) and different levels of drought stress including 0, -6 and -12 barat three replicates was carried out. According to the results, drought stress and kinetin individualy cause to decreases of germination percentage, germination rate, seedling length, seed vigor and seedling dry weight but uses of kinetin in drought stress condition improvment of them. Also, drought stress cause to increases of proline content and catalase activity. Therefore, seed priming with 10 ppm kinetin at drought stress was improved the germination indices of Silybum marianum L.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11919 International Journal of Life Sciences Vol.9(1) 2015 6-11

scholarly journals Responses of Seed Germination, Seedling Growth, and Seed Yield Traits to Seed Pretreatment in Maize (Zea maysL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Yu Tian ◽  
Bo Guan ◽  
Daowei Zhou ◽  
Junbao Yu ◽  
Guangdi Li ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Yield ◽  
Seedling Growth ◽  
Germination Rate ◽  
Seed Priming ◽  
Grain Weight ◽  
Shoot Biomass ◽  
Control Group ◽  
Yield Traits ◽  
Seed Pretreatment

A series of seed priming experiments were conducted to test the effects of different pretreatment methods to seed germination, seedling growth, and seed yield traits in maize (Zea maysL.). Results indicated that the seeds primed by gibberellins (GA), NaCl, and polyethylene glycol (PEG) reagents showed a higher imbibitions rate compared to those primed with water. The final germination percentage and germination rate varied with different reagents significantly (P<0.05). The recommended prime reagents were GA at 10 mg/L, NaCl at 50 mM, and PEG at 15% on account of germination experiment. 15% PEG priming reagent increased shoot and root biomass of maize seedling. The shoot biomass of seedlings after presoaking the seeds with NaCl reagent was significantly higher than the seedlings without priming treatment. No significant differences of plant height, leaf number, and hundred-grain weight were observed between control group and priming treatments. Presoaking with water, NaCl (50 mM), or PEG (15%) significantly increased the hundred-grain weight of maize. Therefore, seed pretreatment is proved to be an effective technique to improve the germination performance, seedling growth, and seed yield of maize. However, when compared with the two methods, if immediate sowing is possible, presoaking is recommended to harvest better benefits compared to priming method.

scholarly journals Pematahan Dormansi Benih Kebiul (Caesalphinia bonduc L.) dengan Berbagai Metode

Akta Agrosia ◽  
2016 ◽  
Vol 19 (2) ◽  
pp. 147-156
Author(s):  
Yesi Uyatmi ◽  
Entang Inoriah ◽  
Marwanto Marwanto
Keyword(s):  
Plant Height ◽  
Root Length ◽  
Germination Rate ◽  
Hot Water ◽  
Germination Capacity ◽  
Hard Seed ◽  
Randomized Design ◽  
Breaking Dormancy ◽  
Different Temperatures ◽  
Hard Seed Coat

Seed of Caesalpinia bonduc L. is difficult to germinate due to the thick and hard seed coat. The research aims to evaluate various methods for breaking dormancy of C. bonduc seed. This study was conducted from December to January 2016 The way to break the dormancy were arranged in Completely Randomized Design. Eleven methods to solve the seed dormancy of C. bonduc were compared. The ways to break the dormancy were soaking in water with different temperatures. The temperatures were 30C for 0 hours, 5 hours, 10 hours, and 15 hours. Other techniques to break the dormancy were soaking in hot water at 10000C for 5 hours, 10 hours, and 15 hours. Warm stratification with 100% air humidity (RH) at 40C for five days, ten days and 15 days also included as treatments. The last method was seed piercing with a needle. The variables observed in this study were germination capacity, germination rate, epicotyl length, plant height, and root length. The results of this study showed that the 11 methods of breaking seed dorman cy significantly affected germination capacity, germination rate, emerging epicotyl, and plant height. Seed piercing classified as the most efficient method as indicated by the value of 100% in germination capacity, 1.43 in germination rate, 13.64 cm in plant height, and 6.16 cm in root length.0

scholarly journals Effect of salinity on seed germination of some tomato (Lycopersicon esculentum Mill.) varieties

2021 ◽  
pp. 76-82
Author(s):  
Getachew Shumye Adilu ◽  
Yohannes Gedamu Gebre
Keyword(s):  
Seed Germination ◽  
Abiotic Factors ◽  
Germination Rate ◽  
Petri Dish ◽  
Germination Percentage ◽  
Control Treatment ◽  
Randomized Design ◽  
Salinity Levels ◽  
Mean Germination Time ◽  
And Control

Salinity adversely affects 20-30% of the irrigated area in the world. Tomato is sensitive to salinity. It is one of the most severe abiotic factors of many agricultural crops and it becoming the main problem in Ethiopia. This study was conducted to evaluate the effects of different salinity levels on the seed germination parameters of tomato varieties. It was laid out in a completely randomized design with three replicates. The treatment included four tomato varieties (Sirinka, Weyno, ARP D2, and Roma VF) and five salinity levels (1 dS m-1, 2 dS m-1, 3 dS m-1, 4 dS m-1, and control). Fifty seeds were placed in a Petri dish over a moistened germination paper for germination and seedlings and allowed to grow for 14 days. The germination rate, speed and energy of tomato seeds were significantly (p < 0.001) affected by the combined effect of variety and salinity. The shortest mean germination time, the highest mean germination rate, and the highest speed of germination were recorded in the ARP D2 variety in the control treatment. The lowest first and last days of germination, and the uncertainty of germination were recorded from ARP D2. However, an increase in the days of germination and in the uncertainty of germination, and a decrease in the germination index and total germination percentage trends were observed with increasing salinity levels. The highest level of salinity (4 dS m-1) affected the germination of tomato varieties. Among the four tested tomato varieties, ARP D2 and Roma VF were tolerant to salinity.

scholarly journals EFFECT OF SEED PRIMING METHODS ON THE GERMINATION OF DETARIUM MICROCARPUM

2021 ◽  
pp. 1-10
Author(s):  
A Abdulrahman ◽  
M Atiku ◽  
A.S Ambursa ◽  
I Umar ◽  
M Muhammed ◽  
...  
Keyword(s):  
Tap Water ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Hot Water ◽  
State University ◽  
Detarium Microcarpum ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Pre Treatment

This study was carried out at Kebbi State University of Science and Technology, Aliero, Kebbi State, to determine the effect of seed priming methods on the germination of Detarium microcarpum. The study was carried out in the Forestry Laboratory of the Faculty of Agriculture. The effectiveness of nine (9) different seed priming methods was assessed, control inclusive. Treatment 1 (TRT1) seeds were soaked in tap water for 48 hours, Treatment 2 (TRT2) seeds were mechanically scarified opposite to microphyle, treatment 3, 4, and 5 (TRT3, TRT4, TRT5) seeds were immersed in H2SO4 for 5, 10 and 15 minutes respectively, treatment 6, 7 and 8 (TRT6, TRT7 and TRT8) seeds were soaked in hot water (100oC) for 5, 10 and 15 minutes respectively and untreated seeds (CNTRL). Completely Randomized Design with three (3) replications was used. The data obtained were analyzed using one-way Analysis of Variance (ANOVA).The result revealed that seeds of D. microcarpum subjected to TRT2, TRT4, TRT5 and TRT8 recorded with highest Final Germination Percentage (FGP 100%) and was found to differ significantly (P<0.05) with other treatment and CNTRL recorded the lowest FGP (60%).Thus, TRT2 was significantly (P<0.05) enhanced the seed and germination rate of D. microcarpum with the highest FGP (100%), lowest MGT (6.67), highest GRI (15.05), highest GE (12.01), lower FDG (5.67) with maximum GI value (900). Based on the result, pre-treatment of T. indica seeds with H2SO4 is recommended for effective germination.

scholarly journals Effects of Hydro and Hormonal Seed Priming on Seed Germination of Milk Thistle under Saline Stress Condition

2014 ◽  
Vol 6 (3) ◽  
pp. 374-380
Author(s):  
Yousef NASIRI ◽  
Parisa FEYZI ◽  
Abdollah JAVANMARD
Keyword(s):  
Salicylic Acid ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Milk Thistle ◽  
Saline Stress ◽  
Nacl Salinity ◽  
Germination Time ◽  
Randomized Design ◽  
Mean Germination Time

Salinity is an abiotic stress which has harmful effects on germination of many plants. Therefore, high germination rate and vigorous early growth under salty soils is preferred. Seed priming is a way to increase salt tolerance of plants. An experiment was conducted to investigate the effect of seed priming on germination of milk thistle under salinity condition. The treatments were 4 levels of seed priming (no priming, distilled water as hydro priming and 0.5 and 1.0 mM salicylic acid) and 5 levels of salinity (0, 40 and 80 mM NaCl and 40 and 80 mM CaCl2). The experiment arranged as a factorial in a completely randomized design (CRD) with three replications. Results showed that salinity decreased germination percentage and germination rate to about 16 and 32% in 80 mM CaCl2 level compared to control, respectively. The highest mean germination time (5.7 day) were belonged to 80 mM CaCl2. Radicle and plumule length significantly decreased by 80 mM NaCl and 40 and 80 mM CaCl2. The lowest seedling weight and seed stamina observed in 80 mM CaCl2. 0.5 mM salicylic acid improved all traits except mean germination time as compared to control.  Salicylic acid (0.5 mM) improved radicle length under 0, 40 and 80 mM NaCl salinity levels as well as increased plumule length at the 0 and 40 mM NaCl salinity conditions.

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