scholarly journals Priming Methods: Alternative Strategy to Improve Seed and Seedling Performance of Soursop (Annona muricata)

2019 ◽  
pp. 1-7
Author(s):  
D. J. A. Najorda ◽  
R. J. G. Rosales
Keyword(s):  
Germination Rate ◽  
Seed Priming ◽  
Seed Vigor ◽  
Seedling Performance ◽  
Randomized Design ◽  
Breaking Dormancy ◽  
Polyethylene Bags ◽  
Hormonal Priming ◽  
Completely Randomized Design ◽  
Experimental Treatments

The evaluation of seed priming methods on the seed and seedling performance of soursop was conducted January 9 to April 3, 2019 in San Nicolas, Ilocos Norte, Philippines.  The study was conducted to investigate the effect of seed priming methods  capable of breaking dormancy; improve germination rate; determine the seed vigor of soursop; and identify the best seed priming method that provide better seedling performance. The experimental  treatments (unprimed and three priming methods, hydropriming, halopriming and hormonal priming) were laid out in Completely Randomized Design with three replications. A     total of 20 polyethylene bags were used per treatment per replication with one seed sown in every bag. Alternative way to improve seed and seedling performance is the use of these seed priming methods. The seed and seedling performance of soursop were significantly affected by priming methods. Primed seeds had higher percentage germination rate (PGR) than unprimed seeds. But numerically, the highest PGR was hydropriming. Hormonal priming produced significantly taller seedlings at 10 and 40 days after emergence (DAE) than unprimed seeds and more leaves per seedling at 30 to 50 DAE. Hormonal, hydropriming and halopriming produced significantly higher fresh weight of seedlings than unprimed seeds.

scholarly journals Effect of seed priming with kinetin on germination indices and proline activity of Silybum marianum L. under drought stress conditions

2015 ◽  
Vol 9 (1) ◽  
pp. 6-11
Author(s):  
Abolfazl Masoumi Zavariyan ◽  
Mojtaba Yousefi Rad ◽  
Mohsen Asghari
Keyword(s):  
Drought Stress ◽  
Germination Rate ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Silybum Marianum ◽  
Randomized Design ◽  
Seedling Length ◽  
Germination Indices

For investigation of the effect of seed priming by kinetin on germination indices and proline activity of Silybum marianum  L. under drought stress, factorial experiment based on completely randomized design with two factor includingdifferent concentrations of kinetin (0, 10 and 20 ppm) and different levels of drought stress including 0, -6 and -12 barat three replicates was carried out. According to the results, drought stress and kinetin individualy cause to decreases of germination percentage, germination rate, seedling length, seed vigor and seedling dry weight but uses of kinetin in drought stress condition improvment of them. Also, drought stress cause to increases of proline content and catalase activity. Therefore, seed priming with 10 ppm kinetin at drought stress was improved the germination indices of Silybum marianum L.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11919 International Journal of Life Sciences Vol.9(1) 2015 6-11

scholarly journals Pengaruh Dosis Minyak Cengkeh Terhadap Populasi Hama Callosobruchus chinensis L., Viabilitas Dan Vigor Benih Dua Kultivar Kacang Hijau Setelah Periode Simpan Tiga Bulan

Agrologia ◽  
2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Siti Syarah Nurbaekah ◽  
Sumadi Sumadi ◽  
Anne Nuraini
Keyword(s):  
Mung Bean ◽  
Germination Rate ◽  
Seed Viability ◽  
Seed Vigor ◽  
Callosobruchus Chinensis ◽  
Clove Oil ◽  
Bean Cultivar ◽  
Randomized Design ◽  
Vigor Index ◽  
Completely Randomized Design

Callosobruchus chinensis L. still become the primary pest of mung bean, it can cause damage until 70 percent. Pest control on mung bean seeds can be done by using anorganic protectant and bio protectant. The aim of the research was to determine the dosage of clove oil that could suppressed the population of weevil C. chinensis L. and maintaining seed viability and seed vigor of two mung bean cultivar after three months storage. The experimental design was Completely Randomized Design with 10 treatments combination of cultivar and clove oil dosage; all treatment were replicated three times. The mungbean seeds were cultivar Vima 1 and Vima 3.  The clove oil dosages used were 0 mL kg-1, 0,5 mL kg-1, 1,0 mL kg-1, 1,5 mL kg-1 and 2,0 mL kg-1. The mungbean result of experiment showed that there were significant effect between cultivar and clove oil dosage on germination rate percentage after three months storage, but no significant effect on weevil population and vigor index. The dosage of 1,5 mL kg-1 clove oil was the better treatment to maintaining seed viabilty andits vigor.Keywords: Mung bean, C. chinensis L., Clove oil, Seed viability and Seed Vigor.

scholarly journals EFFECT OF SEED PRIMING METHODS ON THE GERMINATION OF DETARIUM MICROCARPUM

2021 ◽  
pp. 1-10
Author(s):  
A Abdulrahman ◽  
M Atiku ◽  
A.S Ambursa ◽  
I Umar ◽  
M Muhammed ◽  
...  
Keyword(s):  
Tap Water ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Hot Water ◽  
State University ◽  
Detarium Microcarpum ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Pre Treatment

This study was carried out at Kebbi State University of Science and Technology, Aliero, Kebbi State, to determine the effect of seed priming methods on the germination of Detarium microcarpum. The study was carried out in the Forestry Laboratory of the Faculty of Agriculture. The effectiveness of nine (9) different seed priming methods was assessed, control inclusive. Treatment 1 (TRT1) seeds were soaked in tap water for 48 hours, Treatment 2 (TRT2) seeds were mechanically scarified opposite to microphyle, treatment 3, 4, and 5 (TRT3, TRT4, TRT5) seeds were immersed in H2SO4 for 5, 10 and 15 minutes respectively, treatment 6, 7 and 8 (TRT6, TRT7 and TRT8) seeds were soaked in hot water (100oC) for 5, 10 and 15 minutes respectively and untreated seeds (CNTRL). Completely Randomized Design with three (3) replications was used. The data obtained were analyzed using one-way Analysis of Variance (ANOVA).The result revealed that seeds of D. microcarpum subjected to TRT2, TRT4, TRT5 and TRT8 recorded with highest Final Germination Percentage (FGP 100%) and was found to differ significantly (P<0.05) with other treatment and CNTRL recorded the lowest FGP (60%).Thus, TRT2 was significantly (P<0.05) enhanced the seed and germination rate of D. microcarpum with the highest FGP (100%), lowest MGT (6.67), highest GRI (15.05), highest GE (12.01), lower FDG (5.67) with maximum GI value (900). Based on the result, pre-treatment of T. indica seeds with H2SO4 is recommended for effective germination.

scholarly journals EFFECTIVENESS OF GIBBERELLINS IN BREAKING DORMANCY OF SNAKEFRUIT SEEDS (SALACCA SNAKEFRUIT GEARTNER)

2020 ◽  
Vol 7 (2) ◽  
pp. 91-98
Author(s):  
Enny Adelina ◽  
Nuraeni Nuraeni ◽  
Yohanis Tambing ◽  
Ida Musdalifa
Keyword(s):  
Germination Rate ◽  
Experimental Unit ◽  
Observation Data ◽  
Embryo Axis ◽  
Randomized Design ◽  
High Viability ◽  
Breaking Dormancy ◽  
Completely Randomized Design ◽  
Materials Used ◽  
Radicle Length

This study aims to examine the effectiveness of gibberellins in breaking dormancy to produce high viability snakefruit (Salacca snakefruit Geartner) seeds. The materials used are snakefruit seeds from Tamareja Village, Donggala Regency, sterile sand media, aquades, and GA3 solution. This research was arranged using a completely randomized design (CRD) with one factor, namely the provision of gibberellin, consisting of five levels of concentration, namely P0 = control (without treatment), P1 = 30 ppm and P2 = 40 ppm P3 = 50 ppm P4 = 60 ppm. each treatment was repeated five times so that there were 25 experimental units. Each experimental unit used 20 seeds. Observation data were analyzed using analysis of variance (ANOVA), followed by Tukey' HSD test if the treatment tested showed a significant effect. The results showed that gibberellin administration effectively broke snakefruit seeds' dormancy and produced seeds with high viability. The higher the gibberellins' concentration would increase germination speed plumule length, radicle length, and faster embryo axis emergence. Gibberellin 60 ppm resulted in germination power 98%, germination rate 20.97 days, plumule length 5.52 cm, radicle length 5.15 cm, and embryo axis emergence 9.2 days.

scholarly journals Effect of seed priming by pyridoxine on germination and biochemical indices in Silybum marianum L. under drought stress

2015 ◽  
Vol 9 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Abolfazl Masoumi Zavariyan ◽  
Mojtaba Yousefi Rad ◽  
Mohsen Asghari
Keyword(s):  
Drought Stress ◽  
Germination Rate ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Silybum Marianum ◽  
Randomized Design ◽  
Biochemical Indices ◽  
Germination Indices

In order to investigation of the effect of seed priming by pyridoxine on germination and biochemical indices of Silybum marianum L. under drought stress, factorial experiment based on completely randomized design with two factor includingdifferent concentrations of pyridoxine including 0, 0.02% and 0.04% and different levels of drought stress including 0, -6 and -12 bar at three replicates was performed. The results indicated that drought stress affected measured indices at P<0.01 and pyridoxine significantly affected germination percentage and rate, seed vigor, mean time germination, seedling length and dry weight at P<0.01 and peroxidase activity at P<0.05. Interaction between the drought stress and pyridoxine was significantly (P<0.05) affected of germination rate and seed vigor. The results showed that with increasing drought stress, germination indices were decreased and biochemical indices were increased. Seed priming with pyridoxine cause to improvement of germination indices and decreases biochemical indices. According to the results, seed priming with 0.04% pyridoxine cause to improvement of germination indices at drought stress conditions.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11921 International Journal of Life Sciences Vol.9(1) 2015 17-22

scholarly journals EFFECT OF SEED PRIMING ON GERMINATION OF OKRA (Abelmoschus esculentus var. Arka Anamika)

2021 ◽  
Vol 5 (2) ◽  
pp. 111-114
Author(s):  
Anuj Lamichhane ◽  
Mamata K.C. ◽  
Manisha Shrestha ◽  
Binaya Baral
Keyword(s):  
Seed Germination ◽  
Tap Water ◽  
Germination Rate ◽  
Seed Priming ◽  
Seed Vigor ◽  
Seedling Vigor ◽  
Randomized Design ◽  
Vigor Index ◽  
Seed Hardness ◽  
Positive Effect

Seed priming is an effective, eco-friendly method to promote seed germination and seedling vigor of okra to overcome the reduced and delayed germination in fresh or stored okra seeds caused by seed hardness. An experiment was carried to evaluate the effects of different priming on okra seeds germination and seedling vigor using Arka Anamika variety at Horticulture lab of Agriculture and Forestry University, Rampur, Chitwan, Nepal. Investigation was carried out with 6 treatments (T1: seed priming with tap water, T2: seed priming with 200ppm NAA solution, T3: seed priming with 10% PEG-200 solution, T4: seed priming with 200ppm GA3 solution, T5: seed priming with 5% Trichoderma solution and T6 no priming) with 4 replications in Complete Randomized Design (CRD). Seeds primed with T1 to T5 were soaked for 24 hours and shade dried for 6 hours before sowing. Priming with T4 was found to be best in terms of maximum seed germination (60.12%), seed vigor index (5772.68 cm), mean germination rate (7.53 seeds per day). The highest shoot length (81.40 mm) was observed at T1 whereas enhancement of root length occurred with the priming with T3. All treatments had a significant positive effect on all the germination parameters in comparison to control. The study concluded that GA3 priming enhanced germination as well as seed vigor in okra and hydro priming and tricho-priming can be used as an alternative to GA3 priming among farmers in Nepal.

scholarly journals Effects of seed priming treatments on the germination and development of two rapeseed (Brassica napus L.) varieties under the co-influence of low temperature and drought

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257236
Author(s):  
Zong He Zhu ◽  
Abdul Sami ◽  
Qing Qing Xu ◽  
Ling Ling Wu ◽  
Wen Yin Zheng ◽  
...  
Keyword(s):  
Low Temperature ◽  
Germination Rate ◽  
Seed Priming ◽  
Seed Vigor ◽  
Stress Conditions ◽  
Stem Length ◽  
Activity Levels ◽  
Sod Activity ◽  
Germination Time ◽  
Rape Seedlings

The present study was performed to evaluate the effects of seed priming. This was done by soaking the seeds of two rapeseed cultivars, namely, ZY15 (tolerant to low temperature and drought) and HY49 (sensitive to low temperature and drought), for 12 h in varying solutions: distilled water, 138 mg/L salicylic acid (SA), 300 mg/L gibberellic acid (GA), 89.4 mg/L sodium nitroprusside (SNP), 3000 mg/L calcium chloride (CaCl2), and 30 mg/L abscisic acid (ABA). Primed and non-primed seeds were left to germinate at 15°C and -0.15 MPa (T15W15) and at 25°C and 0 MPa (T25W0), respectively. The results showed that SA, GA, SNP, CaCl2, and ABA significantly improved the germination potential (GP), germination rate (GR), germination index (GI), stem fresh weight (SFW), stem dry weight (SDW), root length (RL), stem length (SL), and seed vigor index (SVI) under T15W15. For ZY15 seeds under T25W0, GA, SNP, CaCl2, and ABA priming reduced the average germination time (96% after 5 days) compared to that of the control (88% after 5 days). For ZY15 seeds under T15W15, SA, SNP, CaCl2, and ABA priming, with respect to the control and water-treated groups, shortened the average germination time (92% after 5 days) compared to that of the control (80% after 5 days). For HY49 seeds under T25W0, GA, SNP, CaCl2, and ABA priming reduced the average germination time (92% after 5 days) compared to that of the control (85% after 5 days). Similarly, for HY49 seeds under T15W15, GA priming shortened the average germination time (89% after 5 days) compared to that of the control (83% after 5 days). These priming agents increased the net photosynthesis, stomatal conductivity, and transpiration rate of rape seedlings under conditions of low temperature and drought stress, while also decreasing intercellular carbon dioxide (CO2) concentrations. Additionally, SA, GA, SNP, CaCl2, and ABA increased superoxide dismutase concentrations (SOD) and ascorbic peroxidase (APX) activities of rape seedlings under stress conditions, while decreasing catalase (CAT) and peroxidase (POD) activities in ZY15 seedlings. In HY49, which is sensitive to low temperature and drought, all priming solutions, except for SNP, led to an increase in SOD activity levels and a decrease in CAT activity levels. Overall, SA, GA, SNP, and CaCl2 increased the concentrations of indoleacetic acid (IAA), GA, ABA, and cytokinin (CTK) in seedlings under stress conditions. Moreover, compared to SA, CaCl2, and ABA, GA (300 mg/L) and SNP (300 mol/L) showed improved priming effects for ZY15 and HY49 under stress conditions.

scholarly journals Development of Liquid Enhancer for Germination of Drought-stressed Oryza sativa subsp. indica Seed cv. MR284

2019 ◽  
pp. 1-9 ◽  
Author(s):  
S. N. Mahadi ◽  
F. Zawawi ◽  
R. Nulit ◽  
M. H. Ibrahim ◽  
N. I. Ab. Ghani
Keyword(s):  
Oryza Sativa ◽  
Seed Germination ◽  
Germination Percentage ◽  
Leaf Length ◽  
Seed Vigor ◽  
Rice Seed ◽  
Combination Treatments ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Selection Of

Aim: This study was conducted to develop liquid enhancer containing KCl, TU, GA, and SA for germination of drought-stressed Oryza sativa subsp. indica cv. MR284 seed. Study Design: All experiments were conducted in a completely randomized design. Two steps were involved in the development process which are to select an ideal concentration for each KCl, TU, GA, and SA, and to find an ideal combination of chemicals from the selection of ideal concentrations acquired in step 1 to form liquid enhancer. There were 20 treatments for step 1 and 9 treatments for step 2. All of these treatments with 6 replicates. Place and Duration of Study: Department of Biology, Faculty of Science, University Putra Malaysia, between June 2018 and December 2018. Methodology: The sterilized rice seed cv. MR284 was stressed in the -1.2 Mpa PEG 6000 solution for three days and germinated in the KCl, TU, GA, and SA solution in a series of concentration for 10 days, in a controlled room. Seed germination was observed daily. Results: In the first step, drought-stressed rice seed showed the best germination performance in the 30 mM of KCl, 2.0 mM of TU, 0.24 mM GA, and 0.5 mM SA. Meanwhile, in the second step, the drought-stressed rice seed showed the best germination performance in the combination of 30 mM KCl + 2.0 mM TU + 0.24 mM GA + 0.5 mM SA. The best germination performance was evaluated by the highest germination percentage (%), germination index, seed vigor, leaf length, root length and biomass. Conclusion: Therefore, the combination treatments of 30 mM KCl + 2.0 mM TU + 0.5 mM SA was found to be the most effective and simplest liquid enhancer formula that has an ability to enhance seed germination of drought-stressed rice cv. MR284 seed.

scholarly journals Sorghum cultivars of different purposes silage

2015 ◽  
Vol 45 (2) ◽  
pp. 298-303 ◽  
Author(s):  
Ricardo Martins Araujo Pinho ◽  
Edson Mauro Santos ◽  
Juliana Silva de Oliveira ◽  
Higor Fábio Carvalho Bezerra ◽  
Poliane Meire Dias de Freitas ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
Randomized Design ◽  
Profile Losses ◽  
Fermentation Profile ◽  
Total Carbohydrates ◽  
Completely Randomized Design ◽  
Experimental Treatments ◽  
Sorghum Bicolor L

This study aimed to assess the fermentation profile, losses and chemical composition of the silages of five sorghum cultivars. A completely randomized design with five replicates was used. The experimental treatments were represented by five sorghum cultivars (Sorghum bicolor (L). Moench): 'BRS Ponta Negra' , 'BRS 610', 'BRS 655', 'BRS 800' and 'BRS 810'. There was variation (P<0.05) for the pH in the cultivars studied, with highest result of pH by 'BRS 610'. The average percentages of lactic acid, acetic acid and propionic acid, differed (P<0.05) among the cultivars, ranging from 32.9 to 59.5, 19.8 to 39.8, and 0.0 to 1.3g kg-1, respectively. The dry matter recovery varied among cultivars (P<0.05), allowing the identification of BRS Ponta Negra, and 'BRS 810' silages, as those which recovered the lowest dry matter, with values of 757.1 and 776.1g kg-1, respectively. There were differences (P<0.05) for dry matter, crude protein, ether extract, neutral detergent fiber, non-fibrous carbohydrates and total carbohydrates contents among the cultivars. It was concluded that, despite the morphological and chemical differences among cultivars, the resulting silages had good fermentation profile.

scholarly journals Temperatures and substrates for germination and vigor of Erythroxylum pauferrense Plowman seeds

2019 ◽  
Vol 41 ◽  
pp. e46030
Author(s):  
João Everthon da Silva Ribeiro ◽  
Arliston Pereira Leite ◽  
Jackson Silva Nóbrega ◽  
Edna Ursulino Alves ◽  
Riselane de Lucena Alcântara Bruno ◽  
...  
Keyword(s):  
Dry Mass ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Randomized Design ◽  
Temperature Regimes ◽  
Seedling Length ◽  
Physiological Quality ◽  
Mean Germination Time ◽  
Completely Randomized Design ◽  
Understory Plant

Erythroxylum pauferrense is an endemic understory plant species of the Northeast Region of Brazil. The species is of great importance to the region and so ecophysiological studies are needed for its preservation. The objective of the present study was to determine the best substrates and temperatures for testing germination and seed vigor of E. pauferrense. An experiment was performed comprising a completely randomized design in a 5 x 4 factorial scheme, with five temperature regimes (20, 25, 30, 35°C constant and 20-30°C alternating) and four types of substrates (paper, vermiculite, sand and commercial substrate). The following characteristics were evaluated: germination percentage, first germination count, germination speed index, mean germination time, seedling length and dry mass (root and shoot). Paper and vermiculite substrates combined with constant temperatures of 20, 25, 30 and alternated between 20-30°C, provide greater seed germination and vigor while 35°C reduces seed physiological quality.

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