scholarly journals ​Influence of Temperature and Salinity Stress on Seed Germination and Seedling Growth of Soybean (Glycine max L.)

2021 ◽  
Author(s):  
Mustafa Çirka ◽  
Ali Rahmi Kaya ◽  
Tamer Eryiğit
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Germination Rate ◽  
Petri Dish ◽  
Negative Effects ◽  
Randomized Design ◽  
Salt Level ◽  
Glycine Max L ◽  
Early Seedling ◽  
Lower Temperature

Background: The high salt level of a germinating environment can lead to reduced, delayed, and even complete inhibition of germination and seeding growth due to osmotic action and/or ion toxicity. Based on this viewpoint, the aim of this study was to investigate germination temperature and salinity effects on germination and early seedling growth of soybean, which can be sown as first and second crops. Methods: Soybean seeds were subjected to NaCl induced saline germinating media prepared in petri dishes under two different germination temperatures (20±2 and 25±2oC). Thirty sterilized seeds per petri dish were sown in ten salt treatments (0, 100, 150, 250, 750, 1000, 2250, 5000, 7500 and 10000 ppm NaCl L-1). The study was carried out according to the completely randomized design with four replications. Result: Lower temperature promoted seed germination, while the high temperature significantly inhibited the seed germination at all NaCl doses tested. As a result, 25oC temperatures, which can only be measured at the time of the second sowing, have been found to negatively affect germination and also increase the negative effects of salt. Due to the moderate tolerance of soybeans to salt stress, the germination rate was positively affected up to 750 ppm NaCl L-1 dose and resulted in severe reductions in subsequent doses. Also, the tolerance of soybean was negatively influenced by the interaction of temperature and NaCl concentration.

scholarly journals The Effect of Osmopriming on Seed Germination and Early Seedling Characteristics of Carum carvi L.

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 94
Author(s):  
Iman Mirmazloum ◽  
Attila Kiss ◽  
Éva Erdélyi ◽  
Márta Ladányi ◽  
Éva Zámboriné Németh ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Petri Dish ◽  
Seed Priming ◽  
Germination Percentage ◽  
Positive Effects ◽  
Carum Carvi ◽  
Early Seedling ◽  
Seedling Characteristics

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.

scholarly journals Seed Germination and Early Seedling Growth of Barley at Negative Water Potentials

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 671
Author(s):  
Tim L. Springer ◽  
Dolores W. Mornhinweg
Keyword(s):  
Seed Germination ◽  
Water Potential ◽  
Seedling Growth ◽  
Germination Rate ◽  
Moisture Stress ◽  
Potential Treatment ◽  
Line Treatment ◽  
Plant Materials ◽  
Early Seedling ◽  
Line P

The impacts of climate change may increase the duration and frequency of droughts, which would have deleterious effects on crop establishment. The objectives of this study were to determine the effects of moisture stress on seed germination and seedling growth of six winter barley (Hordeum vulgare) lines and discuss how the data are used to select plant materials for rapid germination. Twenty-five seeds of each line were germinated in water of potentials of −2.0, −1.6, −1.2, −0.8, −0.4, and 0 MPa for 4- and 7-days. The experimental design was a factorial arrangement of treatments (barley lines and water potential treatments) in a randomized block replicated four times and repeated twice. The 4- and 7-day percentage seed germination varied with line (p < 0.01), water potential treatment (p < 0.01), and line × treatment interactions (p < 0.01). The seed germination rate varied with water potential treatment (p < 0.01), and line × treatment interactions (p < 0.01). The data indicated that enough variation was present to effectively select and breed cultivars for improved germination at a negative water potential. Studying seed germination under moisture stress is the first step for developing an effected selection pressure for identifying plant materials with rapid seed germination.

scholarly journals The Salinity Effects on Germination and Early Seedling Growth of Bean Plants (Bean Phaseolus)

2020 ◽  
Keyword(s):  
Seedling Growth ◽  
Germination Rate ◽  
Petri Dish ◽  
Normal Weight ◽  
Bean Plant ◽  
Control Group ◽  
Early Seedling Growth ◽  
Bean Plants ◽  
Root And Shoot Length ◽  
Early Seedling

The study was conducted at Dilla University, in the laboratory of Biological sciences to see the effects of salinity on germination and early seedling. Objectives:Background: It is estimated that the world 20% of farming land and 50% of cropland is salt stressed and salinity decreases the germination of seed, retards the growth of plant and so it reduces the yield of crop. Objectives: The major objective of this research was to evaluate the effects of salinity on the germination and early seedling growth of bean plants. Specifically, to investigate salt tolerant efficiency, salt effect on seed germination and its rate and growth of bean plant at different concentration of NaCl. Methodology: The experiment required 21 groups, of which 20 of them were experimental with different salt concentrations ranging from 0.05M to 1.00M with 0.05M difference and one control group. Each group contain three bean seed measuring its weight using electronic balance machine, sterilized with 70% Alcohol solution for 15 seconds, rinsed with distilled water, placed in separate Petri dish using a forceps, 50ml of solution were added to each Petri dish with different concentration, all Petri dishes were covered with lids and kept into incubator at room temperature for 17 days, germinated seed were counted, seedlings root and shoot length were measured using a ruler. Finally, the Bean was transferred to non-saline condition, weight of germinated Bean were measured to compare with their normal weight and to determine the effects of salt on seed weight, which was conducted in triplicates. All necessary data was taken, analyzed and interpreted in the form of percentage, graph, tables and figures. Result: When the concentration of salt increased the Bean plant is extremely affected, germination rate decreased and the terminal weights of seed were become lower than the initial weight due to the absorption of water by seed. Conclusions: The rate of germination decrease when the salt concentration increased and late growth of length of root and shoot when the salt dosage is highest and also the weights of seed reduced as concentration of salt is raised. Generally, as the concentration of salt is increased the Bean plant is extremely affected.

scholarly journals Effect of salinity on seed germination of some tomato (Lycopersicon esculentum Mill.) varieties

2021 ◽  
pp. 76-82
Author(s):  
Getachew Shumye Adilu ◽  
Yohannes Gedamu Gebre
Keyword(s):  
Seed Germination ◽  
Abiotic Factors ◽  
Germination Rate ◽  
Petri Dish ◽  
Germination Percentage ◽  
Control Treatment ◽  
Randomized Design ◽  
Salinity Levels ◽  
Mean Germination Time ◽  
And Control

Salinity adversely affects 20-30% of the irrigated area in the world. Tomato is sensitive to salinity. It is one of the most severe abiotic factors of many agricultural crops and it becoming the main problem in Ethiopia. This study was conducted to evaluate the effects of different salinity levels on the seed germination parameters of tomato varieties. It was laid out in a completely randomized design with three replicates. The treatment included four tomato varieties (Sirinka, Weyno, ARP D2, and Roma VF) and five salinity levels (1 dS m-1, 2 dS m-1, 3 dS m-1, 4 dS m-1, and control). Fifty seeds were placed in a Petri dish over a moistened germination paper for germination and seedlings and allowed to grow for 14 days. The germination rate, speed and energy of tomato seeds were significantly (p < 0.001) affected by the combined effect of variety and salinity. The shortest mean germination time, the highest mean germination rate, and the highest speed of germination were recorded in the ARP D2 variety in the control treatment. The lowest first and last days of germination, and the uncertainty of germination were recorded from ARP D2. However, an increase in the days of germination and in the uncertainty of germination, and a decrease in the germination index and total germination percentage trends were observed with increasing salinity levels. The highest level of salinity (4 dS m-1) affected the germination of tomato varieties. Among the four tested tomato varieties, ARP D2 and Roma VF were tolerant to salinity.

scholarly journals Drought Stresses on Seed Germination and Early Growth of Maize and Sorghum

2019 ◽  
Vol 11 (2) ◽  
pp. 310 ◽  
Author(s):  
Mariana S. Queiroz ◽  
Carlos E. S. Oliveira ◽  
Fábio Steiner ◽  
Alan M. Zuffo ◽  
Tiago Zoz ◽  
...  
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
Osmotic Potential ◽  
Germination Rate ◽  
Early Growth ◽  
Root Shoot Ratio ◽  
Randomized Design ◽  
Root And Shoot Length ◽  
Early Seedling ◽  
Effects Of Drought

Seeds of maize (Zea mays L.) and sorghum [Sorghum bicolor (L.) Moench.] were submitted to different osmotic potential levels induced by polyethylene glycol (PEG) with the objective of evaluating the effects of drought stress on seed germination and early seedling growth. Seeds were arranged in paper rolls and soaked in PEG solutions prepared with osmotic potentials 0.0 (control), -0.2, -0.4, and -0.8 MPa and kept into a seed germinator, at 25 &deg;C for 18 days. A completely randomized design in a 2 &times; 4 factorial scheme with four replications of 50 seeds each was used. The results showed that by increasing of the osmotic potential level, germinated seed number, germination rate index, root and shoot length, shoot and root dry matter, and seedling vigor index (SVI) decreased, while mean germination time (MGT) and root: shoot ratio (RSR) increased in both crops. Additionally, the maize was more susceptible than sorghum to drought stress, with germination response declining more rapidly with decreasing osmotic potential. Sorghum crop tolerates water stress of up to -0.2 MPa, without reducing germination of the seeds; however, the growth of shoots and roots are inhibited. Drought stress limits the process of seed germination and early growth of maize seedlings.

scholarly journals Ammonia Volatilization from Urea-Application Influenced Germination and Early Seedling Growth of Dry Direct-Seeded Rice

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Xiaoli Qi ◽  
Wei Wu ◽  
Farooq Shah ◽  
Shaobing Peng ◽  
Jianliang Huang ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Ammonia Volatilization ◽  
Petri Dish ◽  
Ammonia Toxicity ◽  
Early Seedling Growth ◽  
Incubation Experiments ◽  
Poor Seed ◽  
Early Seedling ◽  
Direct Seeded

Poor seed germination and early seedling growth associated with urea-induced soil ammonia volatilization are major constraints in the adoption of dry direct-seeded rice. To directly examine soil ammonia volatilization and its damage to seed germination and early seedling growth of dry direct-seeded rice when urea is applied at seeding, two Petri-dish incubation experiments and a field experiment were conducted. Ammonia volatilization due to urea application significantly reduced seed germination and early seedling growth of dry direct-seedling rice. NBPT significantly reduced ammonia volatilization following urea application. The application of ammonium sulfate, instead of urea at seeding, may mitigate poor crop establishment of dry direct-seeded rice. Root growth of dry direct-seeded rice was more seriously inhibited by soil ammonia volatilization than that of shoot. Results suggest that roots are more sensitive to soil ammonia toxicity than shoots in dry direct-seeded rice system when N is applied as urea at seeding.

scholarly journals Autoallelopathic Potential of Leaflets and Seeds on Seedling Growth of Date Palm (Phoenix dactylifera L.)

2000 ◽  
Vol 5 (1) ◽  
pp. 7 ◽  
Author(s):  
M.O.A. Warrag ◽  
A.S. AI-Wasel
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Date Palm ◽  
Germination Rate ◽  
Phoenix Dactylifera ◽  
Germination Percentage ◽  
Water Soluble ◽  
Water Control ◽  
Early Seedling Growth ◽  
Early Seedling

The autoalle1opathic effects of date palm leaflets and seed aqueous extracts, with -0.05, -0.1 and -0.2 mPa osmotic potentials, on seed germination and early seedling growth were investigated. Final seed germination percentage, seed germination rate, the time of radicle appearance, and plumule length were not affected. In contrast, the cotyledonary sheath and radicle lengths were significantly retarded, whereas the time of plumule appearance was significantly increased by most of the extracts in comparison with the distilled water control. Mannitol solutions, with the same osmotic potential and pH as the extracts, resulted in significantly longer cotyledonary sheaths and radicles and an earlier plumule appearance than their corresponding extracts. Thus, it could be deduced that date palm leaflets and seeds contain water-soluble allelochemicals that could substantially retard early seedling growth of the same species.

Study on Effect of Oil-Contaminated Soil on Seed Germination

2013 ◽  
Vol 864-867 ◽  
pp. 2532-2536
Author(s):  
Fu Ju Xie ◽  
Pu Chen ◽  
Li Hua Mao
Keyword(s):  
Seed Germination ◽  
Crude Oil ◽  
Seedling Growth ◽  
Contaminated Soil ◽  
Germination Rate ◽  
Rice Seedling ◽  
Rice Seed ◽  
Wheat Seed ◽  
Negative Effects ◽  
Oil Concentration

The effects of oil-contaminated soil on plant growth are the focus study area all over the world. In this paper, two kinds of local plant seeds germination and seedlings growth in oil-contaminated soil were studied by germination experiment. Five different oil concentration gradients (0g/kg, 0.2g/kg, 5g/kg, 20g/kg, 40g/kg) were set up in this experiment, and the seed germination rates and bud length of wheat and rice cultivated in thermostat were observed and recorded. The results showed that crude oil took negative effects on wheat seed germination and germinability. With the oil concentration increasing, the germination rate of wheat seed and germinability decreased respectively. Crude oil impacted the seeding growth obviously, even under low oil concentration (20g/kg). Seedling growth almost stopped under high oil concentration such as 40 g/kg. Crude oil promoted germination and germinability of rice seed. The germination rates and germinabilities of three Oil concentrations (0.2 g/kg, 5 g/kg, 20 g/kg, 40 g/kg) were all higher than that in pure soil, further, the higher the oil concentration was, the germination rates and germinabilities were higher. Oil also accelerated the rice seedling growth under three different concentrations.

scholarly journals The CBL-Interacting Protein Kinase NtCIPK23 Positively Regulates Seed Germination and Early Seedling Development in Tobacco (Nicotiana tabacum L.)

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 323
Author(s):  
Sujuan Shi ◽  
Lulu An ◽  
Jingjing Mao ◽  
Oluwaseun Olayemi Aluko ◽  
Zia Ullah ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Seed Germination ◽  
Seedling Growth ◽  
Hypocotyl Elongation ◽  
Interacting Protein ◽  
Knock Out ◽  
Early Seedling Growth ◽  
Tobacco Seedlings ◽  
Early Seedling ◽  
Cotyledon Expansion

CBL-interacting protein kinase (CIPK) family is a unique group of serine/threonine protein kinase family identified in plants. Among this family, AtCIPK23 and its homologs in some plants are taken as a notable group for their importance in ions transport and stress responses. However, there are limited reports on their roles in seedling growth and development, especially in Solanaceae plants. In this study, NtCIPK23, a homolog of AtCIPK23 was cloned from Nicotiana tabacum. Expression analysis showed that NtCIPK23 is mainly expressed in the radicle, hypocotyl, and cotyledons of young tobacco seedlings. The transcriptional level of NtCIPK23 changes rapidly and spatiotemporally during seed germination and early seedling growth. To study the biological function of NtCIPK23 at these stages, the overexpressing and CRISPR/Cas9-mediated knock-out (ntcipk23) tobacco lines were generated. Phenotype analysis indicated that knock-out of NtCIPK23 significantly delays seed germination and the appearance of green cotyledon of young tobacco seedling. Overexpression of NtCIPK23 promotes cotyledon expansion and hypocotyl elongation of young tobacco seedlings. The expression of NtCIPK23 in hypocotyl is strongly upregulated by darkness and inhibited under light, suggesting that a regulatory mechanism of light might underlie. Consistently, a more obvious difference in hypocotyl length among different tobacco materials was observed in the dark, compared to that under the light, indicating that the upregulation of NtCIPK23 contributes greatly to the hypocotyl elongation. Taken together, NtCIPK23 not only enhances tobacco seed germination, but also accelerate early seedling growth by promoting cotyledon greening rate, cotyledon expansion and hypocotyl elongation of young tobacco seedlings.

Sign in / Sign up

Export Citation Format

Share Document