Perbandingan Kualitas Semen Beku Sapi Unggul dan Hubungannya dengan  Tingkat Keberhasilan Inseminasi Buatan pada Sapi Aceh

ABSTRAK. Penelitian ini merupakan penelitian observasional yang dengan rancangan acak lengkap (RAL) dengan 3 kelompok perlakuan jenis semen beku sapi aceh (S1), semen beku sapi bali (S2) dan semen beku sapi simmental (S3). Masing-masing kelompok diulang sebanyak 10 kali. Data kualitas spermatozoa dan angka kebuntingan yang diperoleh dianalisis dengan analisis of variance (ANOVA) dan dilanjutkan dengan uji Duncan dan hubungan kualitas spermatozoa semen beku dengan tingkat kebuntingan diuji dengan regresi berganda. Persentase motilitas, spermatozoas hidup dan TAU sapi aceh dan sapi bali tidak memperlihatkan perbedaan yang nyata (P0,05), namun keduanya berbeda secara nyata (P0,05) dengan sapi simmental. Hasil uji regresi menunjukan ada hubungan yang nyata (P0,05) antara kualitas spermatozoa (motilitas, spermatozoa hidup dan TAU) semen beku dengan tingkat kebuntingan, dengan persamaan regresi adalah Y= - 2,586 + 0,017 X1 - 0,001 X2 + 0,043 X3, nilai koefisien korelasi r = 0,695 dan nilai koefisien determinasi (r2) sebesar 0,483. Keutuhan TAU memiliki hubungan yang lebih kuat (r = 0,695) dibanding dengan motilitas spermatozoa (r = 0,505) dan spermatozoa hidup (r = 0,195) terhadap angka kebuntingan. Disimpulkan kualitas semen beku sapi unggul berpengaruh terhadap tingkat kebuntingan setelah inseminasi pada induk aseptor sapi aceh betina.(Comparison of frozen semen quality of aceh cattle, bali cattle, brahman cattle and simmental cattle and the relationship with the success level of artificial insemination in female aceh cattle)ABSTRACT. This study is an observational study with a completely randomized design (CRD) with 3 groups of frozen semen aceh cattle (S1), bali cattle frozen semen (S2) and simmental cattle frozen semen (S3). Each treatments groups was repeated 10 times. Spermatozoa quality data (motility, live spermatozoa, and intact acrosome) of frozen semen and pregnancy rates obtained were analyzed by analysis of variance (ANOVA) and continued with Duncant test. Percentage motility, live sperm and intact acrosome of aceh cattle, bali cattle and simmental cattle showed no significant differences (P 0.05), but they differ significantly (P 0.05) compared with brahman cattle. Results of regression analysis showed no significant relationship (P 0.05) between spermatozoa quality (motility, live sperm and intact acrosome) with a pregnancy rate of frozen semen, the regression equation is Y = - 2,586 + 0,017 X1 - 0,001 X2 + 0.043 X3, Correlation coefficient value r = 0,695 and coefficient of determination (r2) equal to 0,483. intact acrosome of spermatozoa have a stronger relationship (r = 0.695) compared with sperm motility (r = 0.505) and live spermatozoa (r = 0.195) on the pregnancy rate. It was concluded that the quality of frozen semen had an effect on the pregnancy rate after insemination on female aceh cow.

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Evaluasi Status Reproduksi Sapi Hasil Persilangan Peranakan Ongole dengan Bali

Jurnal Agripet ◽

10.17969/agripet.v21i2.20409 ◽

2021 ◽

Vol 21 (2) ◽

pp. 207-214

Author(s):

Jauhari Efendy ◽

Peni Wahyu Prihandini ◽

Tri Agus Sulistya ◽

Almira Primasari

Keyword(s):

Pregnancy Rate ◽

Semen Quality ◽

Reproductive Organs ◽

Reproductive Organ ◽

Reproductive Status ◽

Bali Cattle ◽

Cattle Reproduction ◽

Rectal Palpation ◽

Macroscopic Analysis

ABSTRACT. Tujuan penelitian untuk mengetahui status reproduksi hasil persilangan sapi peranakan ongole (PO) dan sapi bali (yang selanjutnya dinamakan sapi POBA) jantan dan betina melalui beberapa kegiatan lapang. Penelitian ini bersifat non eksperimental yang dilakukan secara eksploratif; menggunakan 20 ekor sapi berumur antara 32 sampai 36 bulan. Parameter yang diamati meliputi kualitas semen, kondisi organ reproduksi sapi betina dan angka kebuntingan. Hasil penelitian menunjukkan, kualitas semen sapi POBA rendah atau jelek berdasarkan hasil analisis secara mikroskopis maupun makroskopis; namun memiliki libido yang cukup tinggi yaitu rata-rata sekitar 4 menit 25 detik dari mulai mengendus atau didekatkan dengan ternak pemancing (teaser) sampai ejakulasi. Secara umum kondisi organ reproduksi sapi POBA betina normal, sehingga siap dijadikan indukan yang produktif. Sebanyak 66,67% menunjukkan perilaku estrus yang tampak terutama keluar lendir yang terlihat jelas menggantung di bibir vagina bahkan diantaranya mengejar dan/atau menaiki sapi-sapi lain. Namun hasil pemeriksaan kebuntingan (PKB) melalui palpasi rektal menunjukkan tidak ada sapi betina yang bunting. Dapat disimpulkan bahwa sapi POBA jantan infertil, yang diindikasikan melalui hasil pemeriksaan semen dan tidak adanya sapi betina yang berhasil bunting melalui perkawinan alam selama 3 (tiga) bulan. Sedangkan sapi betina POBA memiliki organ reproduksi normal serta menunjukkan ekspresi estrus yang baik. (The evaluation of cattle reproduction status between crosses Ongole Crossbreed with Bali cows) ABSTRAK. The purpose of this research was to determine the reproductive status of the crossbred PO and Bali cattle (hereinafter referred to as POBA cattle) through several field activities. This research is non-experimental and carried out exploratory using 20 cattle aged 32 to 36 months. Parameters observed included semen quality, cows' reproductive organ condition, and pregnancy rate. The quality of POBA bulls semen was low or bad as indicated by the results of microscopic and macroscopic analysis; but had pretty high libido, which was an average of about 4 minutes and 25 seconds, starting to sniff or being brought near to teaser until ejaculation. In general, the reproductive organs of POBA cows were normal so that they were ready to become productive sires. As many as 66.67% showed estrus behavior, especially visible mucus that was hanging on the lips of the vagina, even among them chasing and/or riding other cattle. However, the results of pregnancy examination through rectal palpation showed that there were no pregnant cows. It can be concluded that POBA bulls are infertile, which was indicated by the results of semen examination and the absence of successfully conceived cows through natural mating for 3 (three) months. Meanwhile, POBA cows have normal reproductive organs and show good estrus expression.

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Effect of Different Freezing Rates on Post-Thaw Semen Quality in Yak

Current Journal of Applied Science and Technology ◽

10.9734/cjast/2019/v35i330179 ◽

2019 ◽

pp. 1-6

Author(s):

B. K. D. Borah ◽

B. C. Deka ◽

R. K. Biswas ◽

S. Deori ◽

P. Chakravarty ◽

...

Keyword(s):

Semen Quality ◽

Interaction Analysis ◽

Freezing Rate ◽

Total Incidence ◽

Frozen Semen ◽

Extracellular Release ◽

The Difference ◽

Live Sperm ◽

Rate Interaction

A study was conducted on yak semen to evaluate the effect of different freezing rates on the quality of frozen semen. The semen was frozen following three freezing rates viz., Freezing rate I (@ 5ºC/minute from +4oC to -10oC, @ 40ºC/minute from -10ºC to -100ºC and @ 20ºC/minute from -100ºC to -140ºC), II (@ 4ºC/minute from +4ºC to -12ºC, @ 40ºC/minute from -12ºC to -40ºC and @ 50ºC/minute from -40ºC to -140ºC and III (@ 5oC/minute from +4ºC to -10ºC, @50ºC/minute from -10ºC to -100ºC and @ 20ºC/minute from -100oC to -140ºC). The sperm motility, live sperm, HOST-reacted sperm, total incidence of acrosomal changes and extracellular release of Alanine aminotransferase and Aspartate aminotransferase of yak semen extended in Tris extender differed significantly (P <0.01) between stages of processing and freezing but did not differ significantly between freezing rates and due to stage × freezing rate interaction. Analysis of variance revealed that in frozen semen, a freezing rate significantly (P<0.05) influenced the percentage of live sperm with Mitochondrial Membrane Potential (MMP) but did not significantly influence the percentage of sperm with DNA damage. The percentage of live sperm with MMP in frozen semen was significantly higher for freezing rate III than for freezing rate II but the difference between freezing rates I and III was not significant.

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Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

Jurnal Ilmu dan Teknologi Peternakan Tropis ◽

10.33772/jitro.v6i1.5449 ◽

2019 ◽

Vol 6 (1) ◽

pp. 78

Author(s):

Muhammad Ade Salim ◽

Muhammad Nur Ihsan ◽

Nur Isnaini ◽

Trinil Susilawati

Keyword(s):

Semen Quality ◽

Block Design ◽

Animal Husbandry ◽

Egg Yolk ◽

Coconut Water ◽

Boer Goat ◽

Randomized Block Design ◽

Frozen Semen ◽

Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari 3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1: young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%), while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

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Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients

Animal Bioscience ◽

10.5713/ajas.20.0025 ◽

2021 ◽

Vol 34 (2) ◽

pp. 198-204 ◽

Cited By ~ 1

Author(s):

Suherni Susilowati ◽

Trilas Sardjito ◽

Imam Mustofa ◽

Oky Setio Widodo ◽

Rochmah Kurnijasanti

Keyword(s):

Pregnancy Rate ◽

Dna Fragmentation ◽

Green Tea ◽

Prostaglandin F2α ◽

Green Tea Extract ◽

Control Group ◽

Bull Semen ◽

Frozen Semen ◽

Tea Extract

Objective: The aim of this study was to ascertain the effects of adding green tea extract (GTE) to skim milk-egg yolk (SM-EY) extender on both the quality of post-thawed bull semen and the pregnancy rates of the recipient cows.Methods: Twelve ejaculates from four Simmental bulls, aged 3 to 5 years and weighing 900 to 950 kg, were diluted SM-EY extender, added with 0, 0.05, 0.1, and 0.15 mg GTE/100 mL extender and then frozen. After four weeks storage in liquid nitrogen, the sperm were thawed and evaluated for viability, motility, intact plasma membrane (IPM), and DNA fragmentation. Meanwhile, the estrus cycles of 48 recipient cows were synchronized by intramuscular administration of a single injection of 5 mg prostaglandin F2α. Estrus cows were divided into four equal groups and inseminated artificially 18 to 20 h after the onset of estrus by using semen from each extender group. Pregnancy was diagnosed by measuring serum progesterone levels at 21 days, followed by transrectal palpation 90 days after insemination.Results: The findings revealed that adding 0.1 mg of GTE/100 mL extender produced the highest percentages of sperm viability (70.67%±1.75%), motility (69.17%±1.47%), and IPM (69.23%±1.21%) and the lowest percentage of DNA fragmentation (3.00%±0.50%). The pregnancy diagnosis revealed that all cows (36/36) inseminated using frozen semen in GTE addition extender were pregnant (pregnancy rate 100%), whereas the pregnancy rate of the control group was 83.33% (10/12).Conclusion: It may be concluded that 0.1 mg GTE/100 mL extender yields the best quality of spermatozoa and that all variants doses of GTE in extender produce a higher pregnancy rate among recipient cows.

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Reproductive Performance Of Bali Cattle Cross Breed Brahman Cattle Through Improving The Genetic Quality Of Local Livestock In Special Effort for Cows to be Pregnant Program

Tarjih Tropical Livestock Journal ◽

10.47030/tropical.v1i1.21 ◽

2021 ◽

Vol 1 (1) ◽

pp. 1-7

Author(s):

Irwansyah ◽

Junaedi ◽

Suparman

Keyword(s):

Birth Weight ◽

Reproductive Performance ◽

Artificial Insemination ◽

Secondary Data ◽

Birth Spacing ◽

Calving Interval ◽

Brahman Cattle ◽

Bali Cattle ◽

The Relationship

One of the problems that exist in the community is the lack of identification of the reproductive performance of prospective brooders of Bali cattle that are artificially inseminated. The researcher conducted a research to determine the reproductive performance of Bali Cattle inseminated with Brahman Cattle Semen in SECP (Special Effort for Cows to be Pregnant Program) Program in Kolaka Regency. The parameters observed in this study were; artificial insemination's first age (Years), How many times the artificial insemination (AI) was pregnant (S/C), Length of pregnancy (months), Calving Interval (CI) (months), Birth weight (Kilograms), Correlation of AI's first age with birth weight. Sources of date used in this study are primary and secondary data. Data collection techniques carried out in this study were observation and interviews. The date obtained was analyzed descriptively, analysis of variance, average, and to calculate the relationship between variables was analyzed by correlation. Reproductive performance of Bali Cattle inseminated with Brahman Cattle Semen in SECP Program in Kolaka district include; first age AI 23.63±1.99 months, Service per Conception (S/C) 1.56±0.55, gestational age 9.03±0.38 months, Calving Interval (CI) 12.24±0 .52 months, birth weight 27.85 ± 2.96 and the correlation between AI prime age and birth spacing was 0.77.

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Effect of genotype on semen quality traits of main and reciprocal crossbred chickens

Nigerian Journal of Animal Production ◽

10.51791/njap.v46i2.6 ◽

2020 ◽

Vol 46 (2) ◽

pp. 32-39

Author(s):

U. C. Isaac ◽

U. H. Udoh ◽

R. J. Nosike

Keyword(s):

Reciprocal Cross ◽

Semen Quality ◽

Sperm Concentration ◽

Genetic Constitution ◽

Quality Traits ◽

Breeding Method ◽

Naked Neck ◽

Significant Difference ◽

Live Sperm

Semen quality of cocks is of utmost importance in reproduction. Breeding method, breed, strain and genetic constitution are the major components of the genetic factors capable of affecting the semen quality of cocks. This research seeks to determine the effect of genotype on the semen quality traits of 272 F crossbred cocks produced at day-old by main and 1 reciprocal crossbreeding of 69 Isa Brown and local frizzle feathered, naked neck and normal feathered chickens. The genotypes of the cocks were Isa Brow x frizzle feathered main cross (IBxF), Isa Brown x naked neck main cross (IBxNa), Isa Brown x normal feathered main cross (IBxN), frizzle feathered x Isa Brown reciprocal cross (FxIB), naked neck x Isa Brown reciprocal cross (NaxIB) and normal feathered x Isa Brown reciprocal cross (NxIB). At 36-40 weeks of age, semen was extracted from the cocks by abdominal massage technique and used to evaluate semen volume (SV), sperm motility (SM), sperm concentration (SC) and live sperm (LS) at weekly intervals. Data obtained from the evaluation were subjected to analysisof variance and tested at 5% level of probability. Genotypes differed significantly (P <0.05) in SC at 37-40 weeks and in SM and LS at all ages. There was no significant difference (P>0.05) in SV. Regardless of genotype and age, the mean SV, SM, SC and LS ranged from 0.31±0.02 to 0.24±0.01ml, 71.84±1.33 to 58.75±2.16 %, 3.22±0.02 to2.92±0.02 x109/ml and 71.91±1.37 %, respectively. The NxIB and IBxNa genotypes produced largest numericalsemen volume at 37 and 39 weeks of age respectively. Whereas SC and LS were significantly highest for IBxF and IBxN respectively at week 37, SM was so for IBxN at all ages. Further, the LS of IBxN genotype was significantly highest at 36-38 and 40 weeks of age. The exotic Isa Brown and normal feathered main cross cocks exhibited the best semen quality traits among all genotypes studied and should be selected for improvement of the reproductive characteristics of cocks.

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Reproductive Performance Of Bali Cattle Cross Breed Brahman Cattle Through Improving The Genetic Quality Of Local Livestock In Special Effort for Cows to be Pregnant Program

Tarjih Tropical Livestock Journal ◽

10.47030/tropical.v1i1.133 ◽

2021 ◽

Vol 1 (1) ◽

pp. 1-7

Author(s):

Irwansyah ◽

Junaedi ◽

Suparman

Keyword(s):

Birth Weight ◽

Reproductive Performance ◽

Artificial Insemination ◽

Secondary Data ◽

Birth Spacing ◽

Calving Interval ◽

Brahman Cattle ◽

Bali Cattle ◽

The Relationship

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Effect of preservation time on the quality of frozen semen in indigenous rams

Bangladesh Journal of Animal Science ◽

10.3329/bjas.v44i1.23113 ◽

2015 ◽

Vol 44 (1) ◽

pp. 10-15 ◽

Cited By ~ 1

Author(s):

BBA Mahmuda ◽

Azizun Nesa ◽

BF Zohara ◽

MGS Alam ◽

FY Bari

Keyword(s):

Semen Quality ◽

Egg Yolk ◽

Volume Density ◽

Normal Morphology ◽

Mean Values ◽

Frozen Semen ◽

Significant Difference ◽

The Mean ◽

Fresh Semen

The study was carried out to observe the effects of preservation time on the quality of frozen semen of indigenous rams. Semen was collected using AV once a week from 4 rams. Tris based with 10% egg yolk and 7% glycerol extender was used to extend and freezing the semen. Fresh semen was evaluated for volume, density, mass motility and concentration, and mean values were observed as 0.8±0.2ml, 3.0±0.3, 3.2±0.7, 3.9±0.7×109/ml, respectively. Significant difference (p<0.05) was found for all the parameters among the rams. Mean values of motility, viability and normal morphology percentages were 83.3±4.3%, 88.2±4.4%, 84.2±3.5% in fresh semen while those of chilled semen at 40C were 74.7±2.3, 78.8±4.9 and 79.2±2.9%, respectively. For all the parameters, significant (p<0.05) difference was found among the rams. Frozen sperm motility was observed after thawing at 39-400C for 14-15 seconds. The mean motility, viability and normal morphology percentages after freezing for 24hrs, 7, 15 and 30 days of duration were 39.8±3.1, 41.1±4.3, 40.1±4.1 and 39.4±2.9%; 44.5±2.5, 45.3±2.8, 44.6±2.8 and 43.9±2.8%; 71.0±2.0, 71.7±1.5, 70.7±1.7 and 70.3±1.8%, respectively and values did not decrease significantly (p>0.05) with the increasing time of preservation. Non significantly decrease of the semen quality with advance of preservation time indicates the suitability of the protocol used for freezing of indigenous ram semen in Bangladesh.DOI: http://dx.doi.org/10.3329/bjas.v44i1.23113 Bang. J. Anim. Sci. 2014. 44 (1): 10-15

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Efektivitas Penambahan Vitamin E (alfa-Tokoferol) dalam Medium Pencucian Sperma dengan Sentrifugasi terhadap Kualitas Spermatozoa Sapi Brahman

Jurnal Agripet ◽

10.17969/agripet.v12i2.196 ◽

2012 ◽

Vol 12 (2) ◽

pp. 7-13

Author(s):

Dasrul Dasrul ◽

Rasmaidar Rasmaidar ◽

Abdul Harris

Keyword(s):

Vitamin E ◽

Sperm Quality ◽

Membrane Integrity ◽

Average Percentage ◽

Treatment Groups ◽

Frozen Semen ◽

Brahman Cattle ◽

Sperm Membrane ◽

Better Than

Effect of vitamin E addition (alfa-tokoferol) into sperm washing medium by centrifuge on the quality of Brahman cattle spermatozoa ABSTRACT. The aims of study to determine the effectiveness of the addition of vitamin E in the washing medium by centrifugation on sperm quality Brahman cattle. frozen semen of Brahman cattle, divided into 4 treatment groups addition of vitamin E in the washing medium: 0.0gr/100 ml medium (K0), 0.1gr /100 ml medium (K1); 0.2gr/100 ml medium (K2) and 0.3 g / 100 ml medium (K4), each group was repeated 5 times. Examination of motility, viability and integrity of sperm membrane performed according to WHO standards. The data obtained were analyzed with one-way ANOVA and Duncan test. The average percentage of motility, viability and membrane integrity of spermatozoa in the addition of vitamin E were significantly different (P 0.05) compared to the control. Percentage of motility, viability and membrane integrity of spermatozoa in the group K2 significantly higher (P 0.05) compared with the group K3: K1 and K0. Percentage of motility, viability and sperm capacitation and sperm live on group K3 significantly higher (P 0.05) compared with the K1 and K0. While the percentage motility of spermatozoa in the group K1 higher were not significant (P 0.05) compared with the group K0. The addition of vitamin E in the medium on the process of washing spermatozoa Brahman cattle. The addition of vitamin E 0.2gr/100ml better than vitamin E 0.1gr/100ml and 0.3gr/100ml in maintaining the percentage of motility and live spermatozoa Brahman cattle.

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Identifikasi Bakteri dan Efektivitas Antibiotik dalam Pengencer untuk Mengontrol Pertumbuhan Bakteri pada Semen Sapi Friesian Holstein

jurnal veteriner ◽

10.19087/jveteriner.2019.20.1.140 ◽

2019 ◽

Vol 20 (1) ◽

pp. 140

Author(s):

Muttaqinullah Rabusin ◽

Andriani Andriani ◽

Raden Iis Arifiantini ◽

Ni Wayan Kurniani Karja

Keyword(s):

Semen Quality ◽

Bacterial Species ◽

Enterobacter Cloacae ◽

Bovine Semen ◽

Frozen Semen ◽

Bacterial Content ◽

Pseudomonas Diminuta ◽

Semen Extender ◽

Fresh Semen

This study was designed to investigate the presence of bacterial species in Friesian Holstein (FH) bovine semen at the time of collection, processing and to assess the efficacy of two types of antibiotics combinations; penicillin and streptomycin (PS) and gentamycin, tylosin, lincomycin and spectinomycin (GTLS) in semen extender on bacterial control and quality of semen. For this purpose, three experiments were conducted. In experiment 1, identification of bacterial content in fresh semen which collected from 5 bovine ejaculates. In experiment 2, identification of bacterial content in skimmilk-eggyolk extender which were prepared in artificial insemination center, Lembang, Bandung. In experiment 3, identification of bacterial content in frozen thawed semen. In the result, some of bacterial species were isolated from the bovine semen. The GTLS combination of antibiotics may be incorporated into a freezing extender or protocol without compromising the post-thawed semen quality of FH bull spermatozoa. Three types of bacteria were found in fresh semen; Klebsiella sp., Micrococcus sp., and Pediococcus sp.. Three types of bacteria were found in semen extender; Enterobacter cloacae, Pseudomonas diminuta and Serratia plymutica. Two types of bacteria were found in frozen semen; Enterobacter cloacae and Serratia plymutica. In conclusion, antibiotics PS and GTLS were effective for controlling the growth of bacteria in frozen semen.

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