scholarly journals Evaluasi Status Reproduksi Sapi Hasil Persilangan Peranakan Ongole dengan Bali

2021 ◽  
Vol 21 (2) ◽  
pp. 207-214
Author(s):  
Jauhari Efendy ◽  
Peni Wahyu Prihandini ◽  
Tri Agus Sulistya ◽  
Almira Primasari

ABSTRACT. Tujuan penelitian untuk mengetahui status reproduksi hasil persilangan sapi peranakan ongole (PO) dan sapi bali (yang selanjutnya dinamakan sapi POBA) jantan dan betina melalui beberapa kegiatan lapang. Penelitian ini bersifat non eksperimental yang dilakukan secara eksploratif; menggunakan 20 ekor sapi berumur antara 32 sampai 36 bulan. Parameter yang diamati meliputi kualitas semen, kondisi organ reproduksi sapi betina dan angka kebuntingan. Hasil penelitian menunjukkan, kualitas semen sapi POBA rendah atau jelek berdasarkan hasil analisis secara mikroskopis maupun makroskopis; namun memiliki libido yang cukup tinggi yaitu rata-rata sekitar 4 menit 25 detik dari mulai mengendus atau didekatkan dengan ternak pemancing (teaser) sampai ejakulasi. Secara umum kondisi organ reproduksi sapi POBA betina normal, sehingga siap dijadikan indukan yang produktif. Sebanyak 66,67% menunjukkan perilaku estrus yang tampak terutama keluar lendir yang terlihat jelas menggantung di bibir vagina bahkan diantaranya mengejar dan/atau menaiki sapi-sapi lain. Namun hasil pemeriksaan kebuntingan (PKB) melalui palpasi rektal menunjukkan tidak ada sapi betina yang bunting. Dapat disimpulkan bahwa sapi POBA jantan infertil, yang diindikasikan melalui hasil pemeriksaan semen dan tidak adanya sapi betina yang berhasil bunting melalui perkawinan alam selama 3 (tiga) bulan. Sedangkan sapi betina POBA memiliki organ reproduksi normal serta menunjukkan ekspresi estrus yang baik.  (The evaluation of cattle reproduction status between crosses Ongole Crossbreed with Bali cows) ABSTRAK. The purpose of this research was to determine the reproductive status of the crossbred PO and Bali cattle (hereinafter referred to as POBA cattle) through several field activities. This research is non-experimental and carried out exploratory using 20 cattle aged 32 to 36 months. Parameters observed included semen quality, cows' reproductive organ condition, and pregnancy rate. The quality of POBA bulls semen was low or bad as indicated by the results of microscopic and macroscopic analysis; but had pretty high libido, which was an average of about 4 minutes and 25 seconds, starting to sniff or being brought near to teaser until ejaculation. In general, the reproductive organs of POBA cows were normal so that they were ready to become productive sires. As many as 66.67% showed estrus behavior, especially visible mucus that was hanging on the lips of the vagina, even among them chasing and/or riding other cattle. However, the results of pregnancy examination through rectal palpation showed that there were no pregnant cows. It can be concluded that POBA bulls are infertile, which was indicated by the results of semen examination and the absence of successfully conceived cows through natural mating for 3 (three) months. Meanwhile, POBA cows have normal reproductive organs and show good estrus expression.

2018 ◽  
Vol 18 (1) ◽  
pp. 10-17 ◽  
Author(s):  
Khairul Fatah ◽  
Dasrul Dasrul ◽  
Mohd. Agus Nashri Abdullah

ABSTRAK. Penelitian ini merupakan penelitian observasional yang dengan  rancangan acak lengkap (RAL) dengan 3 kelompok perlakuan jenis semen beku sapi aceh (S1), semen beku sapi bali (S2) dan semen beku sapi simmental (S3).  Masing-masing kelompok diulang sebanyak 10 kali. Data kualitas spermatozoa dan angka kebuntingan yang diperoleh dianalisis dengan analisis of variance (ANOVA) dan dilanjutkan dengan uji Duncan dan hubungan kualitas spermatozoa semen beku dengan tingkat kebuntingan diuji dengan regresi berganda.  Persentase motilitas, spermatozoas hidup dan TAU sapi aceh dan sapi bali tidak memperlihatkan perbedaan yang nyata (P0,05), namun keduanya berbeda secara nyata (P0,05) dengan sapi simmental. Hasil uji regresi menunjukan ada hubungan yang nyata (P0,05) antara kualitas spermatozoa (motilitas, spermatozoa hidup dan TAU) semen beku dengan tingkat kebuntingan, dengan persamaan regresi adalah  Y=  - 2,586 + 0,017 X1 - 0,001 X2 + 0,043 X3, nilai koefisien korelasi r = 0,695 dan nilai koefisien determinasi (r2) sebesar 0,483. Keutuhan TAU memiliki hubungan yang lebih kuat (r = 0,695) dibanding dengan motilitas spermatozoa (r = 0,505) dan spermatozoa hidup (r = 0,195) terhadap angka kebuntingan. Disimpulkan kualitas semen beku sapi unggul berpengaruh terhadap tingkat kebuntingan setelah inseminasi pada induk aseptor sapi aceh betina.(Comparison of frozen semen quality of aceh cattle,  bali cattle,  brahman cattle and simmental cattle and the relationship with the success level of artificial insemination in female aceh cattle)ABSTRACT. This study is an observational study with a completely randomized design (CRD) with 3 groups of frozen semen aceh cattle (S1), bali cattle frozen semen (S2) and simmental cattle frozen semen (S3). Each treatments groups was repeated 10 times. Spermatozoa quality data (motility, live spermatozoa, and intact acrosome) of frozen semen and pregnancy rates obtained were analyzed by analysis of variance (ANOVA) and continued with Duncant test. Percentage motility, live sperm and intact acrosome of aceh cattle, bali cattle and simmental cattle showed no significant differences (P 0.05), but they differ significantly (P 0.05) compared with brahman cattle. Results of regression analysis showed no significant relationship (P 0.05) between spermatozoa quality (motility, live sperm and intact acrosome) with a pregnancy rate of frozen semen, the regression equation is Y = - 2,586 + 0,017 X1 - 0,001 X2 + 0.043 X3, Correlation coefficient value r = 0,695 and coefficient of determination (r2) equal to 0,483. intact acrosome of spermatozoa have a stronger relationship (r = 0.695) compared with sperm motility (r = 0.505) and live spermatozoa (r = 0.195) on the pregnancy rate. It was concluded that the quality of frozen semen had an effect on the pregnancy rate after insemination on female aceh cow.


2018 ◽  
Vol 43 (4) ◽  
pp. 438
Author(s):  
S. Sutiyono ◽  
D. Samsudewa ◽  
A. Suryawijaya

The aim of the study was to examine the synchronization of estrus and artificial insemination in heifers of Ongole grade and Simental-Ongole grade. The research used 19 Ongole Grade (OG) and 18 Simental-Ongole Crossbred (SOC) heifers. This research applied purposive sampling to determine the research population. The criteria to select the population were heifers which have in mature body. The normality of heifer’s reproductive organs were identified by performing rectal palpation, then 50 mg of medroxy progesterone acetate on vaginal sponge was used to synchronized estrus. The estrus heifer was inseminated using frozen semen. The observed parameters were the number of heifers with normal and abnormal reproductive organs, estrus sign and pregnancy. Data were analyzed descriptively. This study showed that OG and SOC with abnormal reproductive organs was 1 heifer (5.56%) and 7 heifers (36.84%), respectively. The responses of estrus synchronization in heifers with normal and abnormal reproductive organs were 94.17% and 100% in OG, and 100% and 42.86% in SOC. Meanwhile, the pregnancy rate was 70.59 and 0% in OG, and 50.00 and 0% in SOC. In conclusion, the number of SOC heifers with abnormal reproductive organs is higher than OG. Estrus synchronization using 50 mg medroxy progesterone acetate increase the amount of estrus and pregnancy in heifers with normal reproductive organs.


2020 ◽  
Vol 44 (4) ◽  
Author(s):  
Riyan Nugroho Aji ◽  
Panjono Panjono ◽  
Ali Agus ◽  
Budi Prasetyo Widyobroto ◽  
Tety Hartatik ◽  
...  

The purpose of this study was to observe the size of reproductive organ and to evaluate the sperm quality of Belgian Blue (BB) crossbreed cattle. This cattle are the crossbreeding result between a female Brahman Cross (BX) cattle inseminated with frozen semen from pure male BB cattle, by Artificial Insemination (IB) method, as a candidate of superior bull. The samples of this study were 2.5 years old (BB1), (BB2), and (BB3) crossbreed cattle. Its process involved general physical examination, genital organ inspection, sperm production, and quality assessment. The data obtained were analyzed through descriptive analysis. The results of this study were the three BB crossbred cattle showed the good appearance of normal reproductive organs with scrotum circumferences of each bull were 40.2 cm, 38.6 cm, and 39.8 cm respectively, and sperm quality of the good and the normal motility values of each bull were 82%, 81% and 80%, viability 84%, 83%, and 86%, and the sperm concentrations of each bull were 2.218 million/ml, 1.986 million/ml and 2.120 million/ml respectively. As a result, it could be concluded that those three BB crossbreed cattle are suitable for being used as a superior bull.


Author(s):  
Rudy Priyanto ◽  
Jefirstson Richset Riwukore ◽  
Ahmad Yani ◽  
Bagus Priyo Purwanto ◽  
Luki Abdullah ◽  
...  

The purpose of this research is to analyze the influence of the policy of the Governor of Nusa Tenggara Timur regarding the quota of beef cattle exports to areas of a national beef consumer toward the development of the Bali cattle population on Timor Island, East Nusa Tenggara. Determination of the location by purposive sampling based on physical criteria (land area), biology (availability and population of Bali cattle), and socio-culture (policy) so that the sampling locations representing the agroecosystem of plantations in Kupang district. The material used was 57 Bali cattle and involved 107 respondents of Bali cattle farmers. Respondents were interviewed using a questionnaire of reproduction status of Bali cattle. In addition to interviews, field observations were also conducted to measure the response of Bali cattle production. This research uses primary and secondary data. Data analysis is done by building a dynamic system using Powersim and interpreting simulation results for the next 30 years.The results showed a significant decrease in the population of Bali cattle over the next 30 years due to the high mortality of calves and female parent, low reproductive status of the female, and the export of cattle from uncontrolled agroecosystems. The results of this study indicate that the beef cattle export quota currently determined by local governments needs to be reviewed. The strategy to improve the quality of Bali cattle production in plantation agroecosystems to support cattle export quotas from Timor Island namely (1) farmers and local governments in striving to increase the population of Bali cattle in the agroecosystem of plantation can be done with a technical approach including prevention of death of cattle to the lowest level (<5% per year) and limitations on cattle exports according to the ability of the region; (2) improving the reproductive quality of Bali cattle through artificial insemination or controlled mating management so that it is more efficient; and (3) the government and stakeholders need to review the policy to determined cattle export quotas and improve the reproductive status of cattle in good breeding practice.


2020 ◽  
Vol 3 (1) ◽  
pp. 40
Author(s):  
Desak Nyoman Dewi Indira Laksmi ◽  
I Gusti Ngurah Bagus Trilaksana

The successful application of reproductive technology requires knowledge of estrus cycle and the ability to detect estrus of Bali cattle. Estrus in cattle lasts for 8-30 hours showed by the acceptance of males for copulation. In this phase, estrogen levels reach maximum levels. High levels of estrogen cause signs of estrus, and changes in the reproductive organs. This study aims to determine estrogen levels during estrus and external genital changes during estrus. This research was an observational analytic study with cross-sectional study design. The samples used in this study were Bali cattle during estrus. The samples had healthy status without any signs of disease. Blood samples were taken through the jugular vein were then centrifuged to obtain serum. The obtained serum was examined for estrogen hormone levels. Mechanical measurements of hormone levels checked by using the Direct Elisa, Double Antibody Sandwich. Blood samples were collected from several SIMANTRI in Mengwi District, Badung Regency. The results showed in Bali cattle external genitals change when estrus, namely redness of the vaginal mucosa, discharge from the vagina and swelling of the vulva. While estrogen levels when estrus is 69.80 pg/ ml. Further research needs to be continued to measure the quality of ovulation after estrus.


Author(s):  
A. Saha ◽  
M. Asaduzzaman ◽  
S. Akter ◽  
F.Y. Bari

Background: Artificial insemination (AI), among all the fundamental systems of animal breeding, has proved to be the best and efficient method for the rapid improvement of livestock for maximum use of superior genetic merit of males on numerous dams. The frozen AI in sheep is, to date, not fulfilling the farmer’s need. Therefore, the present study aimed to determine the pregnancy rate in indigenous ewes with chilled semen preserved at different hours. Methods: Semen was collected from three indigenous rams using an artificial vagina and evaluated for its quality both in the fresh and chilled stage at 12 h, 24 h and 48 h before insemination. Trans-cervical insemination was performed in PGF2α synchronized ewes. Pregnancy was confirmed by ultrasonography at 50-60 days of gestation. Result: The motility, viability and normal sperm values of chilled semen decreased significantly (p less than 0.01) with increasing the duration of preservation. However, the quality of chilled semen was acceptable level for insemination. The pregnancy rate was significantly varied (p less than 0.01) and the higher pregnancy rate (64.28%) was achieved when AI was performed using semen stored at 4oC for 12 h than 24 h (58.33%) and 48 h (50%), respectively. Therefore, increased the duration of preservation time of different chilling process negatively affect the semen quality and the pregnancy rates.


2017 ◽  
Vol 2 (2) ◽  
pp. 13-18
Author(s):  
N. Nirwana ◽  
S. Suparman

This research was conducted by conducting experiments in UPTD laboratory of Livestock and Livestock Feeding Agricultural Service of Sulawesi Barat Province. The purpose of this research is to compare the age of males during March to April 2013. The results of this research are expected to be used as a guideline for the use of Bali Cattle research as a semen producer based on the age of livestock that can be used as one of the criteria in the selection of prospective semen producing males. The research material is fresh semen of Bali Cattle with requirement of 70% individual motility. The research method used is observation method by observing the fresh semen quality of Bali Cattle research which has been classified into 3 groups based on age 3, 5, and 6 years. The data analysis used is the analysis of the variety and the design is Completely Randomized Design. The results showed that the quality of fresh semen in the three age groups (3, 5, and 6 years) where volume: 5.2 ± 1.2; 6.4 ± 1.1; And 6.4 ± 0.8 ml, color: milk, white, pH: 6,4 ± 0,1; 6.4 ± 0.1; And 6.3 ± 0.1, consistency: concentrated; Medium; Concentrated and concentrated, concentrations: 1909.6 ± 418.8; 1223.3 ± 220.8; And 1790.8 ± 240.5 million / ml, and 92.5 ± 2.9%, abnormality: 4.4 ± 1.2; 4.3 ± 1.2; And 3.3 ± 1.0%. The conclusions of this research indicate that the age of males gives a significant effect on the fresh semen volume, fresh semen concentration, gives a very significant effect on fresh semen concentration and does not have a significant effect on fresh semen sperm abnormalities. And a male of Bali Cattle age 3-6 years old in UPTD Livestock Feeding and Animal Feed Agricultural Service of Sulawesi Province has good semen quality for Artificial Insemination.


2021 ◽  
pp. 132-136
Author(s):  
Agung Budiyanto ◽  
Makruf Arif ◽  
Mandala Phivi Whelma Alfons ◽  
Rifia Tiara Fani ◽  
Ardian Faiz Hafid ◽  
...  

The success of beef cattle reproduction development in Indonesia very much depends on the quality of the semen produced by the Indonesian Institute for Artificial Insemination. Good quality bull will benefit farmers, frozen semen producers, and the country in large. This study aimed to determine effect of age and breed on the libido and quality of bull semen collected in the Regional Artificial Insemination Center (RAIC). This study used PO, Simmental, and Brahman Bull in RAIC Yogyakarta, with a total amount of 15, aged 4-7 years old. Semen was collected using an artificial vagina, semen evaluation used a spectrophotometer, and eosin-nigrosin staining method used for sperm viability and abnormalities examination. The result of this study indicated that bull breed did not affect the libido value and the semen quality of bulls (P>0,05). Volume, concentration, motility, and viability in various age groups did not show significant differences (P>0,05), but age groups had a significant effect on sperm abnormalities (P<0,05). The group of bulls aged 5-6 years showed the best quality compared to other groups, with the volume 4,58 ± 1,37 ml, concentration 1,91 ± 0,36 x109/ml, motility 3,80 ± 1,30, viability 89.83 ± 10.69%, and sperm abnormalities 2.51 ± 0.81%. It can be concluded that breed did not affect the bull libido and semen quality, while age had an effect on bull sperm abnormalities.


2018 ◽  
Vol 33 (2) ◽  
pp. 62-70 ◽  
Author(s):  
A Hossain ◽  
MM Islam ◽  
F Naznin ◽  
RN Ferdousi ◽  
FY Bari ◽  
...  

Semen was collected from four rams, using artificial vagina and viability%, motility% and plasma membrane integrity% were measured. Fresh ejaculates (n = 32) were separated by modified swim-up separation using modified human tubal fluid medium. Four fractions of supernatant were collected at 15-minute intervals. The mean volume, mass activity, concentration, motility%, viability%, normal morphology and membrane integrity% (HOST +ve) of fresh semen were 1.0 ± 0.14, 4.1 ± 0.1 × 109 spermatozoa/ml, 85.0 ± 1.3, 89.4 ± 1.0, 85.5 ± 0.7, 84.7 ± 0.5 respectively. There was no significant (P>0.05) difference in fresh semen quality parameters between rams. The motility%, viability% and HOST +ve % of first, second, third and fourth fractions were 53.4 ± 0.5, 68.2 ± 0.3, 74.8 ± 0.3 and 65.5 ± 0.4; 55.5 ± 0.4, 66.2 ± 0.4, 74.5 ± 0.3 and 73.6 ± 0.3 and 66.7 ± 0.5, 66.8 ± 0.5, 65.2 ± 0.4 and 74.7 ± 0.5 respectively. The motility%, viability% and membrane integrity% of separated semen samples differed significantly (P<0.05) between four fractions. The mean motility% and viability% were significantly higher (P<0.05) in third fraction (74.8 ± 0.3%), whereas the mean HOST +ve% was significantly higher (P<0.05) in fourth fraction (74.7 ± 0.5). All quality parameters of separated spermatozoa were significantly (P<0.05) lower than that of fresh semen. The pregnancy rates were higher with fresh semen (71%) in comparison to that of separated sample (57%).Bangl. vet. 2016. Vol. 33, No. 2, 62-70


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