PHENOTYPIC AND GENOTYPIC CHARACTERIZATION OF FIVE ANTIMICROBIAL RESISTANCE GENES ASSOCIATED WITH KLEBSIELLA PNEUMONIAE ISOLATED FROM BURN INFECTION PATIENTS

The current study was carried out for the phenotypic and genotypic characterization of five antimicrobial resistance-associated genes in Klebsiella pneumoniae isolated from burn infection patients. Total one hundred three (103) bacterial samples (strains) were isolated from the 103 burn infection patients admitted at Middle Euphrates Burns Center of AL-Kufa City Iraq. Out of total isolated bacterial samples (103), there were 31 isolates (30%) identified as Pseudomonas sp., 23 isolates (22.3%) as K. pneumonia, 22 isolates as Staphylococcus sps. (21.4%), 11 isolates as E. coli (10.6%), 8 isolates as Acinetobacter sps. (7.8%), 5 isolates as Enterobacter sps. (4.9%), while the lowest prevalence (3 isolates) was reported for the Proteus spp. (3%). The antimicrobial sensitivity test indicated that all isolated K. pneumoniae have resistant (100%) against standard antibiotic Amoxicillin. While Imipenem is the only antibiotic that can inhibit the growth of all 23 isolates. Further, according to the phenotypic detection method, there were 14 isolates (61%) capable of production of extended spectrum beta lactamase (ESBL). Genotypic method to detect the presence of five antibiotic resistance genes by polymerase chain reaction proved that 13 isolates (56.5%) were Tem gene, 18 isolates (78.2%) were positive for Shv gene, 8 isolates (34.7%) were positive Ctxm gene, three isolates (13%) were positive for Oxa gene and 10 isolates (43.7%) positive for AmpC gene. Results of the study can be concluded that K. pneumoniae is the second causative agent that causes burn infection and has higher antibiotics resistance. Extended spectrum beta lactamase of K. pneumoniae was higher prevalence in burn infection and harbored many beta lactamase genes.

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Characterization of Extended-Spectrum β-Lactamases (ESBLs)-Producing Klebsiella Pneumoniae Strains in Captive Giant Pandas

10.21203/rs.3.rs-144915/v1 ◽

2021 ◽

Author(s):

Xiaoyan Su ◽

Xia Yan ◽

Yunli Li ◽

Dongsheng Zhang ◽

Lin Li ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Klebsiella Pneumoniae ◽

Resistance Genes ◽

Giant Panda ◽

Test Method ◽

Giant Pandas ◽

Extended Spectrum ◽

Antimicrobial Resistance Genes ◽

Encoding Genes

Abstract Background: Extended-spectrum β-lactamases (ESBLs)-producing strains of Klebsiella pneumoniae remain a critical clinical concern in the world. However, limited information is available concerning ESBLs-producing K. pneumoniae in giant pandas. The objective of this study was to characterize ESBLs-producing K. pneumoniae isolates from the giant panda. A total of 211 K. pneumoniae isolates were collected from 108 giant pandas housed at the Chengdu Research Base of Giant Panda Breeding, China. Samples were screened for the ESBLs-producing phenotype via the double-disk synergy test method. Result: A total of three (1.42%, n = 211) ESBLs-producing K. pneumoniae strains were identified and subjected to MLST (Multi-locus Sequence Typing) analysis for the characterization of ESBLs-encoding genes, transposons, antimicrobial resistance genes, and antimicrobial susceptibility. Among the three ESBLs-producing isolates, different ESBLs-encoding genes, including blaCTX-M-1, blaSHV, and blaTEM were detected. Three isolates were found to carry transposons (i.e., aac6-ib and tnpU) and antimicrobial resistance genes (i.e., aac6-ib, tnpU, aac6-1, qnrA, and qnrB). Furthermore, these three isolates were resistant to at least 15 antibiotics and had different ST isotypes (ST37, ST290, and ST2640). Conclusion: Effective surveillance and strict infection control strategies should be implemented to prevent outbreaks of ESBLs-producing K. pneumoniae in giant panda.

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Prevalence and Antimicrobial Resistance Patterns of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Blood Cultures in an Istanbul University Hospital

Chemotherapy ◽

10.1159/000224657 ◽

2009 ◽

Vol 55 (4) ◽

pp. 293-297 ◽

Cited By ~ 12

Author(s):

Fatma Koksal ◽

Kenan Ak ◽

Omer Kucukbasmaci ◽

Mustafa Samasti

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Klebsiella Pneumoniae ◽

Blood Cultures ◽

University Hospital ◽

Beta Lactamase ◽

Extended Spectrum Beta Lactamase ◽

Extended Spectrum ◽

Resistance Patterns

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Incidence and Antimicrobial Susceptibility of Escherichia coli and Klebsiella pneumoniae with Extended-Spectrum β-Lactamases in Community- and Hospital-Associated Intra-Abdominal Infections in Europe: Results of the 2008 Study for Monitoring Antimicrobial Resistance Trends (SMART)

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00265-10 ◽

2010 ◽

Vol 54 (7) ◽

pp. 3043-3046 ◽

Cited By ~ 58

Author(s):

Stephen P. Hawser ◽

Samuel K. Bouchillon ◽

Daryl J. Hoban ◽

Robert E. Badal ◽

Rafael Cantón ◽

...

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Klebsiella Pneumoniae ◽

Antimicrobial Susceptibility ◽

Beta Lactamase ◽

Extended Spectrum Beta Lactamase ◽

E Coli ◽

Extended Spectrum ◽

Abdominal Infections

ABSTRACT From 2002 to 2008, there was a significant increase in extended-spectrum beta-lactamase (ESBL)-positive Escherichia coli isolates in European intra-abdominal infections, from 4.3% in 2002 to 11.8% in 2008 (P < 0.001), but not for ESBL-positive Klebsiella pneumoniae isolates (16.4% to 17.9% [P > 0.05]). Hospital-associated isolates were more common than community-associated isolates, at 14.0% versus 6.5%, respectively, for E. coli (P < 0.001) and 20.9% versus 5.3%, respectively, for K. pneumoniae (P < 0.01). Carbapenems were consistently the most active drugs tested.

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Extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae from urinary tract infections: Evolution of antimicrobial resistance and treatment options

Medicina Clínica (English Edition) ◽

10.1016/j.medcle.2018.01.014 ◽

2018 ◽

Vol 150 (7) ◽

pp. 262-265

Author(s):

Gemma Jiménez-Guerra ◽

Víctor Heras-Cañas ◽

Lucía del Carmen Béjar Molina ◽

Antonio Sorlózano-Puerto ◽

José María Navarro-Marí ◽

...

Keyword(s):

Escherichia Coli ◽

Urinary Tract ◽

Antimicrobial Resistance ◽

Klebsiella Pneumoniae ◽

Urinary Tract Infections ◽

Treatment Options ◽

Beta Lactamase ◽

Extended Spectrum Beta Lactamase ◽

Extended Spectrum ◽

Tract Infections

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Serotype profile and molecular characterization of antimicrobial resistance in non-typhoidal Salmonella isolated from gastroenteritis cases over nine years

Journal of Medical Microbiology ◽

10.1099/jmm.0.061416-0 ◽

2014 ◽

Vol 63 (1) ◽

pp. 66-73 ◽

Cited By ~ 10

Author(s):

Neelam Taneja ◽

Suma B. Appannanavar ◽

Ajay Kumar ◽

Garima Varma ◽

Yashwant Kumar ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Molecular Characterization ◽

Resistance Genes ◽

Salmonella Enterica ◽

Third Generation ◽

Beta Lactamase ◽

Extended Spectrum Beta Lactamase ◽

First Time ◽

Third Generation Cephalosporins

Resistance to third-generation cephalosporins in non-typhoidal Salmonella (NTS) is emerging worldwide. We report the occurrence of extended-spectrum beta-lactamase (ESBL) phenotypes in 53.4 % of NTS isolated over a period of nine years from gastroenteritis cases. ESBL and AmpC co-production was observed in 21 % of the isolates. Occurrence of bla CTX-M-15 and bla CMY-2 resistance genes was observed in 11.6 % and 37 % of the isolates respectively. Overall, Salmonella enterica serovar Senftenberg was the predominant serovar carrying bla CTX-M-15 and bla CMY-2 resistance genes. We report for the first time from India, one isolate each of S. enterica serovar Thompson, S. enterica serovar Infantis and S. enterica serovar Newport, carrying the bla CTX-M-15 gene. We also report for the first time from India, a case of gastroenteritis due to S. enterica serovar Thompson.

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Emergence and rapid global dissemination of CTX-M-15-associated Klebsiella pneumoniae strain ST307

10.1101/352740 ◽

2018 ◽

Author(s):

Kelly L. Wyres ◽

Jane Hawkey ◽

Marit A.K. Hetland ◽

Aasmund Fostervold ◽

Ryan R. Wick ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Klebsiella Pneumoniae ◽

Carbapenem Resistance ◽

Beta Lactamase ◽

First Report ◽

Extended Spectrum Beta Lactamase ◽

Esbl Gene ◽

Resistance Determinants ◽

Extended Spectrum ◽

Globally Distributed

AbstractRecent reports indicate the emergence of a new carbapenemase producing Klebsiella pneumoniae clone, ST307. Here we show that ST307 emerged in the mid-1990s (nearly 20 years prior to its first report), is already globally distributed and is intimately associated with a conserved plasmid harbouring the blaCTX-M-15 extended-spectrum beta-lactamase (ESBL) gene plus other antimicrobial resistance determinants. Our findings support the need for enhanced surveillance of this widespread ESBL clone in which carbapenem resistance is now emerging.

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