scholarly journals ANTI-PROLIFERATIVE POTENTIAL OF Carica papaya LEAVES ON BREAST CANCER CELLS – MCF-7

2021 ◽  
Vol 9 (5) ◽  
pp. 678-686
Author(s):  
Sasikala Mariyappan ◽  
◽  
Sivakumar Ramalingam ◽  
Lavanya Murugan ◽  
Renuka Saravanan ◽  
...  
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Dna Fragmentation ◽  
Carica Papaya ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Free Radical Scavenging ◽  
Phytochemical Analysis ◽  
Proliferative Effect ◽  
Mcf 7

The study's objective is to identify the phytoconstituents and determine the anti-cancer potential of Carica papaya leaves against the MCF 7 cell line. Chloroform, ethyl acetate, and methanol extracts of C. papaya leaves were prepared by cold maceration method and qualitative phytochemical analysis was performed. The anti-proliferative effect of these extracts was determined by 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and apoptotic assay by acridine orange/ethidium bromide staining method on MCF 7 cells. The effect of the extracts, with different concentrations, on DNA fragmentation, was also performed on MCF 7 cells. Qualitative analysis revealed the presence of alkaloids, flavonoids, terpenoids, steroids, saponins, tannins, glycosides, phenols, anthraquinones, proteins, and carbohydrates. Chloroform, methanol, and ethyl acetate extracts of C. papaya leaves were observed with potential DPPH free radical scavenging activity with 72%, 75%, and 78% respectively. Of these extracts, the chloroform extract (72%) was found to possess a more free radical scavenging effect against DPPH and also showed a dose-dependent effect, the maximum at 100µg/ml, on DNA fragmentation in MCF 7 cells. Further, chloroform extract showed a maximum anti-proliferative effect on MCF-7 cells with IC50 at 22±1.5µg/ml, whereas methanol and ethyl acetate extract at 30±0.5 µg/ml and 28±0.5 µg/ml respectively. Increased apoptosis in MCF 7 cells was observed with an increased concentration of chloroform extract of C. papaya. From the results of this study, it can be concluded that leaf extract of C. papaya found to possess an anti-proliferative effect and antioxidant potential and it could be due to the presence of rich secondary metabolites of the plant.

scholarly journals Free-Radical Scavenging Activity (FRSA) of Secondary Metabolite Extracted from Indonesian Eucheuma spinosum

ALCHEMY ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 1-6
Author(s):  
Nurul Inayah ◽  
Masruri Masruri
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Red Seaweed ◽  
Ic50 Values

IIndonesia is the biggest country in the production of red seaweed of Eucheuma spinosum. The red seaweed has bioactive compounds that have a potential activity such as phenolic compounds as well as carrageenan and pigments. This paper reported phytochemical analysis of E. spinosum harvested by a local farmer in Sumenep Island, East Java and free-radical scavenging activity (FRSA) derived from 2,2’-diphenyl-1-picrylhdrazyl (DPPH) for several organic solvents. For extraction, dried powder is added with 5.0 mL of various solvents following by ultra-sonication assisted extraction for 30 minutes. The extract was separated by centrifugation for phytochemical analysis and radical scavenging evaluation. The prospecting of dichloromethane, ethyl acetate, and n-hexane extracts indicated the potency for radical scavengers. Alkaloids, terpenoids, and saponins were secondary metabolites that indicated the presence in the extracts. The best IC50 value was presented by ethyl acetate extracts (384,86 ppm) with 38.78% for 50 ppm, while IC50 values of n-hexane, methanolic, dicloromethane extracts were 410.12, 677.76 and 685.08 ppm, respectively. Keywords: Eucheuma spinosum, phytochemical analysis, radical scavenging activity

scholarly journals GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS, IN VITRO CYTOTOXIC AND ANTIOXIDANT EFFICACY STUDIES ON CLEOME GYNANDRA L. (LEAVES): A TRADITIONAL DRUG SOURCE

2017 ◽  
Vol 10 (10) ◽  
pp. 84
Author(s):  
Renuka Saravanan ◽  
Brindha Pemaiah ◽  
Mahesh Narayanan ◽  
Sivakumar Ramalingam
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Cleome Gynandra ◽  
Mcf 7

  Objective: This study was aimed to assess the phytoconstituents, cytotoxic, and antioxidant efficacy of ethyl extract of Cleome gynandra leaves.Methods: Qualitative phytochemical analysis with different solvent extracts was performed. Quantitative and gas chromatography-mass spectrometry (GC-MS) analysis of the extract was performed with ethyl acetate extract. The cytotoxic effect of the ethyl acetate extract was determined by 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide (MTT) assay on Michigan Cancer Foundation-7 (MCF-7) cells using taxol as standard and free radical scavenging ability using 1,1-diphenyl-2-picrylhydrazyl (DPPH).Results: Leaves extracts with different solvents revealed the presence of alkaloids, cardiac glycosides, flavonoids, phenols, and tannins. GC-MS analysis of ethyl acetate of the plant leaves showed the presence of n-hexadecanoic acid. The IC50 value of the ethyl acetate extract was found to be 90.2 μg/ml on MCF-7 cell line, and the extract was found to possess significant DPPH free radical scavenging activity.Conclusion: From the results, we conclude that the C. gynandra extract possesses antioxidant and antiproliferating activity against MCF-7 cells.

scholarly journals PHYTOCHEMICAL ANALYSIS AND IN VITRO FREE RADICAL SCAVENGING ACTIVITY OF SUCCESSIVE EXTRACTS OF ALYSICARPUS VAGINALIS VAR. NUMMULARIFOLIUS (DC.) MIQ

2017 ◽  
Vol 9 (6) ◽  
pp. 231
Author(s):  
Susan Kurian ◽  
Leenamma Joseph ◽  
Josekumar V. S.
Keyword(s):  
Nitric Oxide ◽  
Hydrogen Peroxide ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Phytochemical Analysis ◽  
Scavenging Activity

Objective: The present study was carried to evaluate free radical scavenging activity and phytochemical analysis of successive extraction with hexane (AVH), ethyl acetate (AVE) and methanol (AVM) of Alysicarpus vaginalis var. nummularifolius (DC.) MIQ., a herb used in home remedies.Methods: The entire plant was dried and powdered, successively extracted (soxhlet apparatus) and concentrated using rotary vacuum evaporator. The extracts were quantitatively analyzed for phytochemicals like total phenolic, flavonoids, alkaloids and carbohydrates following standard methods. The free radical scavenging activity was evaluated with DPPH (1, 1-diphenyl-2-picrylhydrazyl), nitric oxide radical and hydrogen peroxide scavenging reactions.Results: The quantitative phytochemical studies of the crude extracts showed that AVM extract with highest phenolic, flavonoid, alkaloid and carbohydrate content which is followed by AVE and AVH. The IC50 values of AVH, AVE and AVM for scavenging DPPH, nitric oxide and hydrogen peroxide were 589.21±0.09 µg/ml, 254.65±0.08 µg/ml and 261.40±0.08 µg/ml; 533.81±0.09 µg/ml, 362.07±0.09 µg/ml and 456.36±0.08 µ/ml; 464.66±0.08 µg/ml, 380.29±0.08 µg/ml and 367.6±0.08 µg/ml respectively. Conclusion: The present study revealed that AVM extracts having high polyphenolic compounds when compared with AVE and AVH. The free radical scavenging reaction was greater in ethyl acetate and methanol extracts. This suggests that due to the high antioxidant reactions, these extracts can be useful for the treatment of oxidative stress–related diseases.

scholarly journals EVALUATION OF ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAVES OF PIPER UMBELLATUM

2017 ◽  
Vol 10 (2) ◽  
pp. 274
Author(s):  
Sethupandian Geetha ◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Frap Assay ◽  
Radical Scavenging Properties

Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum.  Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents

scholarly journals Antioxidant and antimicrobial activities against Enterobacter cloacae of extracts of Eclipta alba Hassk.

2016 ◽  
Vol 19 (4) ◽  
pp. 76-83
Author(s):  
Trang Thi Xuan Dai ◽  
Anh Thi Tu Vo
Keyword(s):  
Free Radical ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Enterobacter Cloacae ◽  
Free Radical Scavenging ◽  
Antioxidant Vitamin ◽  
Eclipta Alba

This study was subjected to investigate antimicrobial and antioxidant properties of Eclipta alba extracts (methanol, hexane, chloroform and ethyl acetate extracts). The antioxidant property of the extract was assessed by 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Kirby-Bauer method was used to determine the antibacterial activity against Enterobacter cloacae of the extracts. The extracts of Eclipta alba were tested against E. cloacae. The highest antibacterial potentiality was exhibited by the ethyl acetate extract of E. alba leaves, means of zones of bacterial growth inhibition are 26.3 mm at a concentration of 32 μg/mL. DPPH free radical scavenging effect of the extracts was compared with standard antioxidant vitamin C. The highest antioxidant activity was exhibited by the ethyl acetate extract of E. alba leaves. The result also showed that the DPPH scavenging activity of ethyl acetate extract from leaves of E. alba was high (EC50 = 419.38 μg/mL). However, this result was 18.99 times lower than that of vitamin C (EC50 = 22.08 μg/mL).

scholarly journals Fractionation, Phytochemical Screening and Free Radical Scavenging Capacity of Different Sub-Fractions from Pituranthos scoparius Roots

2020 ◽  
Vol 10 (3) ◽  
pp. 133-136
Author(s):  
Ahlem Karbab ◽  
Kamel Mokhnache ◽  
Lekhmici Arrar ◽  
Abderahmane Baghiani ◽  
Seddik Khennouf ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Health Benefit ◽  
Natural Antioxidants ◽  
Chloroform Extract ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Potential Health

The purpose of this study was to prepare three sub-fractions from Pituranthos scoparius roots (PSR), characterize their phytochemicals contents and to investigate their free radical scavenging activity by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl scavenging activities. Tannins, flavonoids, steroids, and other bioactive compounds were found in the different sub-fractions. The Ethyle acetate extract (EAE) and chloroform extract (ChE) exhibited the highest antioxidant activity using ABTS (17.8 ± 0.87 µg/mL and 18.15 ± 0.68 µg/mL), respectively. Whereas, Crude extract (CrE) have been presented strong hydroxyl scavenging activity (14.9 ± 0.8 µg/mL). This study indicates that PSR extracts has potent free radical scavenging, and may prove to be of potential health benefit as well as additional resources for natural antioxidants. Keywords: Medicinal plant; phytochemical screening; sub-fractions, free radical scavenging.

scholarly journals PHYTOCHEMICAL CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF THE NORTHERN MOROCCAN SPECIES: WITHANIA FRUTESCENS L.

2019 ◽  
pp. 276-279 ◽  
Author(s):  
ABDELFATTAH EL MOUSSAOUI ◽  
FATIMA ZAHRA JAWHARI ◽  
DALILA BOUSTA ◽  
AMINA BARI
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Mineral Elements ◽  
Free Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Phytochemical Analysis ◽  
Total Polyphenols

Objective: In this study, we were interested in qualitative, quantitative phytochemical characterization and evaluation of the antioxidant capacity of the total extracts of a plant from northern Morocco, the species selected for this study is Withania frutescens. Materials and Methods: Analysis of mineral elements by inductive coupling plasma-atomic absorption spectroscopy (ICP-AES), phytochemical screening, polyphenol and tannin assays, evaluation of antioxidant activity by the 1,2-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method and reducing power. Results: The plant contains alkaloids, saponins, tannins, mucilages, and coumarins. It has a relatively high content of total polyphenols and tannins of 19.53±0.018 μg genetic generalized epilepsy/mg MS and 6.258±0.062 μg Eqcat/mg MS, respectively. The analysis of mineral elements by ICP-AES shows that our species is rich in mineral elements which are calcium, magnesium, and sodium, and it is devoid of metallic elements such as nickel, lead, cadmium, and cobalt. The evaluation of antioxidant activity by the DPPH free radical scavenging method shows that the half maximal inhibitory concentration of the tested extracts has an antiradical activity of about 0.056±0.008 μg/ml for the ethanol extract and 0.213±0.004 μg/ml for the methanol extract compared to the butylated hydroxytoluene value of 0.009±0.0004 μg/ml which was used as a reference. The reducing capacity test shows that methanolic extract has a high antioxidant capacity (0.213±0.006) compared to ethanolic extract (0.043±0.004) but remains low compared to ascorbic acid (0.003±0.0004) which was used as reference. Conclusion: Phytochemical analysis of W. frutescens shows that this plant is rich in high quantities of alkaloids, saponins, mucilage, tannins, and coumarins. It contains an average amount of total polyphenols and tannins that confer significant antioxidant activity to the plant studied.

scholarly journals Biological Activities of Isolated Compounds from Three Edible Malaysian Red Seaweeds, Gracilaria changii, G. manilaensis and Gracilaria sp.

2016 ◽  
Vol 11 (8) ◽  
pp. 1934578X1601100
Author(s):  
Yosie Andriani ◽  
Desy Fitrya Syamsumir ◽  
Tee Ching Yee ◽  
Faizah Shaharom Harisson ◽  
Gan Ming Herng ◽  
...  
Keyword(s):  
Free Radical ◽  
Xanthine Oxidase ◽  
Cell Lines ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Marine Macroalgae ◽  
Diffusion Method ◽  
Red Seaweeds ◽  
Gracilaria Changii ◽  
Mcf 7

Gracilaria species are red marine macroalgae that are found abundantly in Malaysia. Gracilaria changii from Morib, Selangor, G. manilaensis and Gracilaria sp. from Gelang Patah, Johor were used in this study. Five compounds were successfully isolated and identified as hexadecanoic acid (1), cholest-5-en-3-ol (2), 2-hydroxymyristic acid (3), cholesteryl myristate (4) and 1-(4'-methoxyphenyl)-3-(2”,4”,6”-trihydroxyphenyl)-3-hydroxypropanone (5) based on spectral data analysis (IR, UV, GC-MS, 1H NMR, 13C NMR, HMQC and HMBC). All compounds isolated were tested for cytotoxicity (MTT assay for HL-60 and MCF-7 cell lines), and antibacterial (disc diffusion method), antioxidant (DPPH free radical scavenging assay and xanthine oxidase inhibitory assay) and acetylcholinesterase inhibitory (AChE) activity (TLC bioautographic method). Compounds 1 and 3 exhibited strong cytotoxic activity against HL-60 and MCF-7 cell lines. Compound 5 showed high antioxidant activity in both the DPPH free radical scavenging and xanthine oxidase inhibition assays. Compound 1 showed positive activity for AChE inhibitory with a minimum inhibition dose of 0.625 μg sample. All compounds demonstrated antibacterial activity producing 8 to 14 mm inhibition zones. A positive control was applied to all bioassays and experiments were performed with three replicates. Results demonstrated that three edible red seaweeds are rich sources of bioactive compounds with potential application for pharmaceutical purposes.

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