scholarly journals The effect of implantation materials based on equine and bovine xenogenic bone extracellular matrix on the formation of extracellular neutrophilic traps (experimental study)

2020 ◽  
Vol 26 (4) ◽  
pp. 571-575
Author(s):  
O.V. Diuriagina ◽  
◽  
M.V. Chepeleva ◽  
E.I. Kuznetsova ◽  
M.A. Kovinka ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Bone Tissue ◽  
Bone Defect ◽  
Experimental Period ◽  
Cylindrical Shape ◽  
Nuclear Transformation ◽  
Bovine Bone ◽  
Group I ◽  
Group Ii ◽  
Equine Bone

Purpose To study the effect of osteoplastic materials based on the extracellular xenomatrix of bovine and equine bone tissue on the formation of neutrophil extracellular traps (NETs) in the peripheral blood of rabbits in the early post-operative period after implantation. Materials and methods The study was carried out on 18 male rabbits of the Soviet Chinchilla breed, aged from 8 months to 1.2 years, weighing from 3.0 to 4.5 kg. A perforated bone defect of a cylindrical shape measuring 2 x 6 mm in the distal metaphysis of the right and left femurs was modeled in the animals. The rabbits were divided into three groups, six animals each. In group I, the bone defect was left unfilled; in group II, the defect was filled with a bovine bone tissue xenomatrix, and an equine bone tissue xenomatrix was implanted in group III animals. The implantation material had the appearance of a yellowish crumb with a particle size of 0.5– 1 mm. Blood smears stained according to Romanovsky-Giemsa were used for counting extracellular neutrophil traps (NETs). The percentage of neutrophils that passed the stages of nuclear transformation and emitted free chromatin into the extracellular space in the form of network-like structures was calculated. Results On days 3–7 of the experiment, the number of NETs increased in the early stages of NETosis in all groups. There were no significant differences between the groups. In group I, on days 7 and 14, the number of early forms of NETs (stages 1a and 1b) returned to the values of the preoperative period. In groups II and III, normalization of NETs (stage 1a) did not occur, and the content of NETs (stage 1b) returned to the initial level only by day 30 of the experiment. On days 3, 7, 14, the number of mature NETs increased in all groups. The highest values were noted in group II, where the bovine xenogeneic matrix was implanted. Conclusion Implantation materials based on the extracellular matrix of equine and bovine xenogeneic bone stimulate excessive formation of early NETs on days 14–30 of the experimental period in response to xenotransplantation. Xenomaterials of bovine bone tissue, in comparison with xenomaterials of equine bone tissue, induce a more pronounced inflammatory reaction in the nearest time after defect filling, which is manifested by higher production of mature NETs on days 3–14 of the experiment.

scholarly journals Effect of anti-inflammatory agents on the integration of autogenous bone graft and bovine bone devitalized matrix in rats

2008 ◽  
Vol 23 (2) ◽  
pp. 140-148 ◽  
Author(s):  
Roberto Antoniolli da Silva ◽  
Djalma José Fagundes ◽  
Andréia Conceição Milan Brochado Antoniolli Silva ◽  
Karin Ellen Sisti ◽  
Themis Maria Milan Brochado de Carvalho ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Defect ◽  
Saline Solution ◽  
Diclofenac Sodium ◽  
Bovine Bone ◽  
Collagen Formation ◽  
Group I ◽  
Significant Difference ◽  
Anti Inflammatory ◽  
Group Ii

PURPOSE: To study the repair of bone defect filled with autograft or bovine bone devitalized matrix in rats under anti-inflammatory action. METHODS: Two hundred and forty Wistar rats were distributed to two groups of 120 animals each. A 2mm-diameter defect was created in the femoral diaphysis. Animals of Group I had the bone defect filled with autograft and those of Group II, with bovine bone devitalized matrix. Animals of each group were redistributed to four subgroups according to the intramuscular administration of anti-inflammatory drug or saline solution: A - diclofenac sodium; B - dexamethasone; C - meloxicam; D - saline solution. Evaluation periods were 7, 14 and 30 days. Histological evaluation consisted of quantifying the inflammatory process, the bone neoformation, the collagen formation and the presence of macrophages. RESULTS: Animals of Group I did not show significant difference considering inflammatory reaction. Significant and progressive increase of bone neoformation was observed in both groups. The animals that received meloxicam and autograft showed less collagen formation at 14 and 30 days. The number of macrophages was higher in Group II than in Group I. The animals treated with dexamethasone and saline solution did not show statistically significant difference. CONCLUSIONS: Diclofenac sodium and meloxicam delayed bone graft repair and dexamethasone did not interfere in it.

The Role of Bone Grafts in Preventing Medication-Related Osteonecrosis of the Jaw: Histomorphometric, Immunohistochemical, and Clinical Evaluation in Animal Model

2021 ◽  
pp. 194338752110483
Author(s):  
Jonathan Ribeiro da Silva ◽  
Maria Cristina de Moraes Balbas ◽  
Caroline Águeda Corrêa ◽  
Manuella Zanela ◽  
Roberta Okamoto ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Tissue ◽  
Clinical Evaluation ◽  
Tricalcium Phosphate ◽  
Bone Grafts ◽  
Osteonecrosis Of The Jaw ◽  
Control Group ◽  
Bovine Bone ◽  
Beta Tricalcium Phosphate ◽  
Group I

Objective: To evaluate the effects of inorganic bovine bone graft (Lumina Bone, Criteria, Brazil) and beta-tricalcium phosphate (β-TCP) graft (ChronOS, Synthes, Brazil) in rats with the risk of developing post-extraction medication-related osteonecrosis of the jaw (MRONJ). Methods: Eighteen male Wistar rats weighing 350 to 450 g were induced to develop MRONJ using zoledronic acid for 5 weeks. In the sixth week, the right maxillary first molar was extracted. The animals in Group I (G1) did not receive bone grafts after tooth extraction, while Group II (G2) animals received inorganic bovine bone grafts, and Group III (G3) animals received beta-tricalcium phosphate (β-TCP) grafts. Clinical evaluation and histomorphometric and immunohistochemical analyses were performed. ANOVA and Tukey’s statistical tests were used and a level of significance was considered to be 5%. Results: In the clinical evaluation, animals from G2 and G3 did not present clinical manifestations of osteonecrosis, unlike the control group (G1) animals, which presented necrotic bone tissue exposure in all samples. In the histomorphometric evaluation, animals in G3 showed greater formation of bone tissue (66%) and less formation of bone lacuna (18%) than animals in G1 (58%/32%) and in G2 (59%/27%) ( P < 0.05). Moderate (++) immunostaining was observed in G2 and G3 for RANKL, TRAP, and OC, while G1 showed moderate (++) labeling for OC and mild (+) immunostaining for TRAP and RANKL. Conclusions: Greater formation of bone tissue and fewer bone lacunae were found in animals treated with β-TCP. In clinical evaluation, bone graft groups presented with the clinical manifestation of MRONJ and showed higher intensity of immunostaining for TRAP and RANKL. Despite the limitations of experimental animal studies, the results of this work may assist in the development of future clinical research for the prevention of MRONJ.

scholarly journals Study of Mechanisms of Pulmonal Tissue Treatment in Destructive Tuberculosis

2019 ◽  
pp. 20-23
Author(s):  
O. S. Shevchenko ◽  
I. A. Ovcharenko ◽  
L. D. Todoriko
Keyword(s):  
Extracellular Matrix ◽  
Connective Tissue ◽  
Pathogen Resistance ◽  
Antituberculosis Drugs ◽  
Pulmonary Tb ◽  
Lung Tuberculosis ◽  
Group I ◽  
Sputum Conversion ◽  
Group Ii ◽  
Tissue Reorganization

Introduction. Disruption of the extracellular matrix is one of the most important pathological events in the formation of residual changes in lung tissue in tuberculous inflammation. Аim. Investigation of the dynamics of connective tissue reorganization in lung tuberculosis with a different profile of pathogen resistance to antituberculosis drugs. Materials and methods. 124 patients with new cases of pulmonary TB: group I (n=84) – patients with multidrugresistant TB; group II (n=40) – patients with pulmonary TB with preserved sensitivity of the pathogen to drugs. Results. After 3 months of treatment in group I remained 11.9 % bacterial excretion. After 3 months of treatment in group II there was a decrease in the activity of macrophages against the background of the termination of bacterial excretion and a decrease in the levels of MMP‑9, OSS and AS, which indicates a slowdown of the destruction processes against the background of low fibrotic activity. After 3 months of treatment in group I, fibrosis was active, accompanied by an increase in OSS levels and a decrease in OS levels. Slow sputum conversion in group I was accompanied by a slow (8.2 %) decrease in the ratio of MMP‑9 / TIMP‑1 due to further increase in the level of MMP‑9. Conclusions. In group I, there was a significantly higher activity of the destruction processes, while in group II, there was a lower activity of the fibrotic processes.

scholarly journals Prevention of inflammation, pain and loss of height of marginal bone tissue at the stage of dental implantation and direct prosthetics

2020 ◽  
Vol 104 (5) ◽  
pp. 36-43
Author(s):  
P. Leonenko ◽  
◽  
Yu. Kokoieva ◽  
Keyword(s):  
Bone Resorption ◽  
Bone Tissue ◽  
Dental Implant ◽  
Group Iii ◽  
Dental Implantation ◽  
Analgesic Therapy ◽  
Group I ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Group Ii

Summary. Inflammation and pain can lead not only to a deterioration in the patient’s condition, but also to such local consequences as: bone resorption, loss of soft tissue volume, an increase in the wound healing time and patient rehabilitation in general. Inflammation-induced bone resorption in the area of implantation with direct prosthetics, caused by the activity of cytokines and prostaglandins, negatively affects the entire result of treatment of dentition defects in general. This is because the quality and quantity of bone tissue is one of the key points in the success of prosthetics on dental implants, therefore, pharmacological support of dental implantation and direct prosthetics is an important component of treatment. Purpose: to investigate the effect of inflammation and pain on peri-implant bone tissue at the stages of dental implantation and direct prosthetics and scientifically substantiate pharmacological support in order to prevent inflammatory bone resorption. Materials and methods. A clinical prospective study of 57 patients was carried out at the stage of dental implantation and direct prosthetics with randomization according to the type of pharmacological accompaniment: 1) group I received anti-inflammatory therapy in the form of a balanced inhibitor of COX-1, COX-2 and 5-LOG – nimesulide and analgesic therapy – dexketaprofen trometamol; 2) group II received anti-inflammatory therapy – a selective COX-2 inhibitor – meloxicam and analgesic therapy – ibuprofen; 3) group III did not receive anti-inflammatory and analgesic therapy due to contraindications to the use of non-steroidal anti-inflammatory drugs. Patients of groups I, II, III underwent: clinical, radiological and functional research methods by monitoring the state in dynamics. Results. According to the data obtained, the indices of pain intensity in group I were significantly lower (p < 0.05) as of 1 and 2-d days, compared with groups II and III. The stabilization of inflammatory processes in group I was recorded on the 2-d day. There was a significant decrease (p < 0.05) in the signs of the inflammatory process in patients of group I on the 3rd day (3.01±0.11 units), and on the 7-th day – their complete absence (1.12±0.23 units). In group II, significant regression of inflammation was noted on the 4th day (3.14±0.12 units), and on the 7-th day, minimal signs were observed (2.04±0.17 units). A decrease in signs of inflammation in group III occurred from the 5th day (3.31±0.28 units), and inflammatory phenomena were observed on the 7th day of the study (2.65±0.27 units). In group I, there was a significant stop in the loss of stability of the connection between the bone tissue and the dental implant on the 20-th day (65.08±1.03 points). As of the 25-th day, in patients of group I of the study, there was significantly higher (p < 0.05) indicators of the coefficient of stability of the implant (66.21±1.40 points) in relation to group II of patients (62.93±0.94 points), in who used selective COX-2 inhibitors, and group III (62.90±0.75 points), where NSAID’s were not used. The loss of marginal bone around the dental implant during the study period in group I was 0.5±0.23 mm CI, in group II – 1.1±0.34 mm, in group III – 1.3±0.28 mm. Side effects in group I of the study were recorded in 5.3 % of patients taking drugs nimesulide and dexketoprofen, and in 15.8 % of those in group II who took drugs meloxicam and ibuprofen. Conclusions. Complex pharmacological support of dental implantation and direct prosthetics on implants in the treatment of dentition defects, consisting of perioperative analgesia – dexketoprofen trometamol, as well as nimesulide for anti-inflammatory therapy, made it possible to influence the trauma-induced bone resorption in the implantation area by controlling inflammation. As a result, on the 20-th day in the patients of the group I of the study, a significant stop was noted in the loss of stability of the connection of the bone tissue and the dental implant (65.08±1.03 units), and on the 25-th day of the study in the group I it was found significantly higher (p < 0.05) indicators of the coefficient of stability of the implant and less loss of height of marginal bone tissue in relation to groups II and III of patients. This pharmacological complex made it possible to achieve stabilization of pathological processes in soft tissues – stopping the formation of edema on the 2-d day, a significant decrease (p < 0.05) of signs of the inflammatory process on the 3-d day (3.01±0.11 points) and to implement effective pain prevention at the stages of dental implantation and direct prosthetics.

scholarly journals DYNAMICS OF INDICATORS OF MARKERS OF BONE METABOLISM IN BONE DEFECT REPLACEMENTFABRIC EQUIVALENTS OF BONE TISSUE ON THE BASIS OF MMSC-AT

2019 ◽  
pp. 5-9
Author(s):  
A.V. Bambuliak ◽  
P.P. Perebujnis ◽  
S.V. Tkachuk ◽  
A.V. Javorskiy
Keyword(s):  
Bone Tissue ◽  
Bone Defect ◽  
Stromal Cells ◽  
Bone Repair ◽  
Statistical Significance ◽  
Bone Defects ◽  
Vegf Gene ◽  
Group Vi ◽  
Experimental Animals ◽  
Group I

Bone tissue is one of the most commonly transplantable and inferior to blood components only. The "gold standard" is still considered to be an autologous bone transplant, but this method has some drawbacks associated with additional surgery. The alternative is the use of allogeneic bone, but in this case there is a risk of immunological rejection of the donor bone and the possibility of infection of the recipient. A promising area for the replacement of volumetric bone defects is the creation of bioimplants based on synthetic biocompatible materials impregnated with growth factors that stimulate bone remodeling, or the settlement of stem (multipotent) cells. Most often, mesenchymal multipotent stromal cells are used for settlement. The aim of the study: to find out the level of expression of BGP, Col 1, VEGF genes as indicators of bone repair and mineralization by replacement of bone defects with tissue equivalents of bone tissue based on multipotent mesenchymal stromal cells from adipose tissue. Materials and methods.The experiment was conducted on the Wistar line rats, weighing 200-250 grams, which were divided into VI groups. A bone defect model was formed in the parietal section of the skull of rats. The formed defect implanted the harvested material. Reverse transcription PCR (OG-PCR) was used to quantify mRNA expression for the BGP-bone marker gla protein; VEGF is a vascular endothelial growth factor and Sol 1 (type 1 collagen). Total RNA was isolated from bone tissue by a standard phenol-chloroform-guanidinisothiocyanate method using a set of RNA-Extra reagents to isolate RNA from blood, tissues, cell cultures in several steps according to the manufacturer's recommendations. The data obtained were processed using Bio-Rad CFX Manager 3.0.The obtained results are processed statistically. Research results and their discussion.The highest number of copies of the BGP gene, at 90 days of observations, was determined in experimental animals of the II and III experimental groups (6,280 ± 0,70 and 6,380 ± 0,72, respectively), the number of which did not differ in statistical significance from the data in the animals of the control group, р˃0.05. However, in animals of IV, V and VI groups the number of copies of BGP-gene was 1.5, 1.4 and 1.6 times smaller in relation to the data in intact rats, p0.05, and did not differ in statistical significance , p1 - p4˃0.05. After 3 months of studies determined the decrease in the activity of the production of the gene Col 1. It was noted that the value of the parameter studied in all study groups was equal to the data in intact animals of group I, p0,05 and among themselves, p1 - p4 р0,05, and ranged from the lowest values in group VI rats - 5,192 ± 0,74, and maximum values in group II animals - 6,200 ± 0,88. After 90 days of experimental studies, the high activity of VEGF gene production in experimental animals, which was equal to the data in control rats, p0,05 was investigated. The maximum activity of VEGF gene production was determined in animals of groups IV, II and VI and ranged from 1,200 ± 0,21 copies in group VI to 1,260 ± 0,22 copies in group IV. Conclusions.Thus, according to molecular genetic analysis of the number of cDNA copies encoding BGP, Col 1 and VEGF genes, the most positive changes that contributed to bone repair, mineralization, and complete closure of the defect were observed with the replacement of bone defects in IVa VI specimens.

scholarly journals Effect of topical Insulin administration on bone defect healing in diabetic rats

2020 ◽  
Vol 23 (2) ◽  
Author(s):  
Hany Mahmoud Mahmoud ◽  
Hanyabd-elhamed Sheriff ◽  
Abd-elnasserabd-Elmoala Ismael ◽  
Alaaabd-alla Zaki
Keyword(s):  
Bone Formation ◽  
Bone Defect ◽  
Healing Process ◽  
Bone Defects ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Group I ◽  
Group Ii ◽  
Experimental Group

Objective: insulin has been reported to possess anabolic effect on bone. Topical application of insulin in bone defects in diabetic rats has not been investigated. The objective of this study was to evaluate histologically the effect of topical administration of insulin for the restoration of tibial bone defects in diabetic rats. Materials and Methods: Sixteen adult male albino diabetic rats were grouped into two equal groups, group I (diabetic control) which had not received any graft, group II (experimental) which was diabetic and had received topical insulin loaded on PVP (Polyvinylpyrrolidone). Specimens were harvested on days seven and twenty eight days after surgical procedures, prepared and examined histologically by H&E (haematoxylin and eosin) stain, there were wide histological differences between the groups of this study along the different intervals of the study.Results:The histological results demonstrated that there was obvious retardation in resolving the inflammatory phase, organization of the blood clot and bone formation in the diabetic control group I than the experimental group II along the different intervals of this study. Moreover, there was great acceleration in granulation tissue formation, organization and bone formation in experimental group II which received the insulin PVP. Discussion: The enhancement in bone healing process was due to the effect of insulin which accelerates the bone regeneration by means of inflammation resolve, angiogenesis, proliferation and successive differentiation of mesenchymal cells. Conclusion: Topical applications of insulin in bone defects enhance new bone formation in diabetic rats.KEYWORDSInsulin; Bone defect; Tibia; Diabetes; Rats.

scholarly journals Value of color Doppler ultrasonography and radiography for the assessment of the cancellous bone scaffold coated with nano-hydroxyapatite in repair of radial bone in rabbit

2012 ◽  
Vol 27 (2) ◽  
pp. 148-154 ◽  
Author(s):  
Rasoul Rahimzadeh ◽  
Abbas Veshkini ◽  
Davood Sharifi ◽  
Saeed Hesaraki
Keyword(s):  
Bone Defect ◽  
Cancellous Bone ◽  
Doppler Ultrasonography ◽  
Local Reaction ◽  
Proper Time ◽  
Color Doppler ◽  
Color Doppler Ultrasonography ◽  
Bone Scaffold ◽  
Group I ◽  
Group Ii

PURPOSE: To evaluate the osteo-regenerative capacity of proprietary bone grafting material as a bone defect filler and osteogenetic stimulation to speed up bone healing too. METHODS: Eighteen adult male New Zealand white rabbits were anesthetized and a segmental full thickness bone defect of 10 mm in length was created in the middle of the right radial shaft in all rabbits. They were divided into two groups of 9 rabbits. Group I was considered as control and the fractured site was fixed using finger bone plate with 4 screws, whereas the cancellous bone scaffold coated with Nano-Hydroxyapatite was used to fill the gap after fracture fixation in Group II. Radiography, two dimensional and color Doppler ultrasonography were done before and after creating defects and on 0, 15, 30, 60 and 90 days to evaluate local reaction as far as new blood vessels network and callus formation are observed. RESULTS: On the radiographs during the whole process, bone repair in Group I was not as perfect as those in Group II samples and trace of internal callus filled the gap incompletely in 60 days in Group I, whereas in Group II internal callus almost was formed on 30 days and in addition intercortical callus was seen supporting to cover and filled the gap completely in this group in 60 day; Sonographic findings confirmed the protrusion of newly formed blood vascular network in 30 days in Group I and from 15 days in Group II and remarkably increased till end of observation period. CONCLUSIONS: The nano-hydroxyapatite with more features and shorter in time, made possible the reconstruction of bone tissue and alternative techniques as well as previous bone graft, also radiography and ultrasonography are reliable techniques to trace local reaction at proper time.

scholarly journals SAT0080 COMPARATIVE ANALYSIS OF FRAGILITY FRACTURE IN PATIENTS WITH ELDERLY ONSET RHEUMATOID ARTHRITIS AND YOUNG ONSET RHEUMATOID ARTHRITIS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 974.1-975
Author(s):  
P. Kozhevnikova ◽  
I. Dydykina ◽  
P. Kovalenko ◽  
A. Lila
Keyword(s):  
Rheumatoid Arthritis ◽  
Bone Tissue ◽  
Fragility Fracture ◽  
Disease Duration ◽  
Young Onset ◽  
Group I ◽  
Elderly Onset ◽  
The Mean ◽  
Group Ii ◽  
Elderly Onset Rheumatoid Arthritis

Background:Rheumatoid arthritis (RA) is a chronic autoimmune disease, characterized by erosive arthritis and systemic organ involvement. Chronic inflammation, long RA disease duration, decreased physical activity, immobilization, glucocorticoids use lead to local (periarticular osteoporosis) and generalized loss of bone tissue, decrease in bone mineral density, contravention of microarchitectonics and increased risk of fragility (low-energy) fractures. The structure and density of bone tissue at elderly onset RA, in addition to the above factors, are affected by comorbid diseases, sex steroids level decrease and molecular changes in bone tissue, specifically attributed to aging, followed by increased bone resorption. Fractures reduce the quality of life, have a negative impact on the course of the underlying and concomitant diseases.Objectives:Сompare frequency of fragility fracture in patients with elderly onset rheumatoid arthritis and young onset rheumatoid arthritis.Methods:We included 474 patients with RA diagnosed at 25– 78 years old and fulfilled the American College of Rheumatology (formerly American Rheumatism Association) classification criteria for RA revised in 1987. The patients were divided into two groups depending on age at the RA-onset: The first group (group I) included 217 patients with young onset RA (at 25 to 44 years), second group (group II) included 66 patients with elderly onset RA (aged ≥60 years). The distribution of patients into groups was consistent with the age classification of the World Health Organization. In total, it was selected 283 RA patients. The mean age in group I was 50.4 years, in group II - 71.2 years. The mean age at the onset of RA in group I was 35.0 years, in group II - 66.2 years.; RA duration was 14.4 years and 4.6 years, respectively. Long-term history of glucocorticoids use (for more than 3 months) was observed in 50% of patients in group I, and in 42% of patients in group II.Results:40 (18%) patients in group I and 17 (26%) patients in group II had fragility fractures. Among patients with fragility fracture, 23 (57,5%) patients in group I and 6 (35%) patients in group II received glucocorticoid therapy for more than 3 months. Two or more fractures in history had 16 (40%) in group I, and 3 (18%) in group II.Conclusion:The frequency of fragility fracture in the study groups was comparable (p> 0.05), despite the age of patients. But, the frequency of refractures was higher in patients with RA-onset at young age, which, apparently, is a consequence of long RA disease duration and use of glucocorticoids.Disclosure of Interests:None declared

Extracellular matrix-inspired BMP-2-delivering biodegradable fibrous particles for bone tissue engineering

2015 ◽  
Vol 3 (42) ◽  
pp. 8375-8382 ◽  
Author(s):  
Young Min Shin ◽  
Wan-Geun La ◽  
Min Suk Lee ◽  
Hee Seok Yang ◽  
Youn-Mook Lim
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Bone Defect

A heparin conjugated fibrous particle resembling the structure of an extracellular matrix was developed. The BMP-2 loaded particles promoted osteogenic differentiation and healing of a bone defect, in vitro and in vivo.

scholarly journals Are We Economically Efficient Enough to Increase the Potential of in Vitro Proliferation of Osteoblasts by Means of Pharmacochemical Agents?

2016 ◽  
Vol 10 (1) ◽  
pp. 420-430 ◽  
Author(s):  
Mehmet Isyar ◽  
Seyit Ali Gumustas ◽  
Ibrahim Yilmaz ◽  
Duygu Yasar Sirin ◽  
Hacı Bayram Tosun ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Surface Morphology ◽  
Bone Tissue ◽  
The European Union ◽  
In Vitro Proliferation ◽  
Group I ◽  
Significant Difference ◽  
Osteogenic Effect ◽  
Trisodium Salt ◽  
Group Ii

Background: The aim of this study was to test the necessity of using expensive and unaccesible pharmacological-chemical agents in the proliferation of bone tissue cultures and in the induction of mineralized matrix formation to increase the osteogenic effect. Methods: For this purpose, human primary cell cultures were prepared and then divided into two groups. Whereas the cells in group I were fed with an osteoblast stimulator medium containing Dulbecco’s Modified Eagle Medium (DMEM) and β-glycerophosphate, the cells in group II were fed with DMEM containing dexamethasone and 2-phospho-L-ascorbic acid trisodium salt. Both groups were evaluated in terms of viability, toxicity, and proliferation and then compared in terms of cell surface morphology through inverted light and environmental scanning electron microscopy. In addition to immunoflow cytometric analyses, the effects of alkaline phosphatase activities were evaluated using the spectrophotometric method to examine the osteoblastic activities. Costs were calculated in the currency of the European Union (Euros). The Tukey Honestly Significant Difference test was used to reach the statistical evaluation of the data after the analysis of variance. Results: It was reported that the level of the alkaline phosphates was higher in group I compared to group II. It was observed that the surface morphology quality, the number of living cells, and proliferation were higher in group II and that the results were deemed statistically significant. Conclusion: It was found that the 2-phospho-L-ascorbic acid trisodium salt and dexamethasone mixture was as effective as the expensive commercial kits on the osteogenic effect on human primary bone tissue.

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