scholarly journals Study on Bacillus Isolated from Intestine of Persian Sturgeon (Acipenser persicus) Comparing with Commercial Probiotics

2016 ◽  
Vol 60 ◽  
pp. 59-65
Author(s):  
Mahsa Tabari ◽  
Khashayar Tabari ◽  
Hassan Karimzadegan ◽  
Mehrdad Mohammadi
Keyword(s):  
Fish Larvae ◽  
Probiotic Bacteria ◽  
Control Treatment ◽  
Special Importance ◽  
Persian Sturgeon ◽  
Acipenser Persicus ◽  
Significant Difference ◽  
Special Growth ◽  
Experimental Treatments ◽  
Commercial Probiotics

At present, probiotic bacteria are proposed as an important alternative for antibiotic elimination and for providing renewable products through biological control in cultivation systems of marine fish larvae. The aim of this study is to compare the effects of native and commercial probiotics on growth parameters of Persian sturgeon (Acipenser persicus) larvae fed with controlled biomar diet. Experimental diets with three probiotic bacterial mixture including commercial lactobacillus (L. plantarum and L. rhamnosus), commercial Bacillus (B. subtillis and B. licheniformis) and Bacillus isolated from intestine of Persian sturgeon (B. mycoides) were completed in two levels (6.1 and 7.2 log CFU in each g of feed) and were fed to larvae of Persian sturgeon in six experimental treatments. The control treatment was fed with diet without supplementation. The experiment was done in randomized design. In all the treatments, feed conversion index was increased considerably and had significant difference with control treatment and in all the experimental treatments influenced by probiotics were higher than that of control treatment (P<0.05). The highest rate means of special growth (1.84±0.21%) and thermal growth index (3.44±0.37%) was obtained respectively in treatment C1 and C2 (isolated from intestine). Maximum special growth rate (SGR %) were obtained from C1 (4.69±0.71%) and B2 (4.62±0.87%, commercial Bacillus). In addition, native probiotic bacteria had better performance than the non native commercial species as well as selection of suitable probiotic (natural) with respect to fish species, region and condition of culture plays a special importance role in enhancing the yield of Persian sturgeon larvae.

Exploration of the Timing of Introduction of Design Heuristic Cards to Early Design Brainstorming Sessions by Interdisciplinary Student Teams

2020 ◽  
Author(s):  
Abimelec Mercado Rivera ◽  
José E. Lugo
Keyword(s):  
Design Thinking ◽  
Idea Generation ◽  
Control Treatment ◽  
Early Design ◽  
Design Heuristics ◽  
Student Teams ◽  
Significant Difference ◽  
Experimental Treatments ◽  
The Individual ◽  
College Of Engineering

Abstract This research focuses on improving the outcome of idea generation sessions of interdisciplinary student teams working in the early design stages of a product or solution by measuring the effect of incorporating Design Heuristics Cards at different points of ideation sessions that adhere to the brainstorming guidelines. Using the design thinking methodology, an open-ended challenge was given to the participating teams for a Brainstorming exercise divided into a fifteen-minute individual segment followed by a thirty-minute team ideation segment. Three experimental treatments were designed where Design Heuristics Cards were introduced at different points of the ideation exercise: the start of the individual ideation segment, the start of the team ideation segment, or the second half of the team ideation segment. A fourth control treatment did not introduce the cards at any point but used the Brainstorming guidelines throughout. The metrics observed were Fluency, Novelty, Feasibility, and Market Fit of the ideas generated by the students. Eighty-four students participated in the experiment, with 58.3% being from majors in the College of Engineering, 28.6% from majors in the College of Business Administration, 7.1% from majors in the College of Arts and Sciences, and 6.0% from majors in the College of Agriculture. No significant difference was found among the experimental treatments; however the results are not considered final due to the explorative nature of the study. Recommendations are made on future work and possible improvements to the experiment.

Biuret and urea in concentrates for milking cows

1968 ◽  
Vol 35 (2) ◽  
pp. 191-202 ◽  
Author(s):  
R. Waite ◽  
M. E. Castle ◽  
J. N. Watson ◽  
A. D. Drysdale
Keyword(s):  
Milk Production ◽  
Water Consumption ◽  
Control Treatment ◽  
Average Value ◽  
The United Kingdom ◽  
Significant Difference ◽  
Feeding Value ◽  
Negative Balance ◽  
Experimental Treatments ◽  
Do So

SummaryTwo experiments, one with 18 and one with 21 cows, were made to compare the feeding value for milk production of concentrates in which the nitrogen normally supplied by oil cake was completely replaced by either biuret or urea. In expt 1 the urea and biuret contributed 52% of the total nitrogen in the concentrate and in expt 2 they contributed 43%. Hay was fed as the sole roughage. The estimated energy intake in expt 1 was 102–104% of the Woodman standard and in expt 2 it was 108–109%.In both experiments milk production was about 10% less on the urea and biuret treatments than on the control treatment. There was no significant difference in milk yield between the biuret and the urea treatments. The percentages of fat and protein in the milk from the urea and biuret treatments were higher than from the control treatment, but the weights of these milk constituents were slightly lower than from the control treatment. Liveweight changes and water consumption were not affected by the experimental treatments. In expt 2 an intake of nitrogen about 20% lower than is often recommended in the United Kingdom did not appear to depress milk yields. Three cows that had received biuret concentrates in expt 2 continued to do so during a nitrogen balance trial lasting 3 weeks. Each cow was slightly in negative balance, the average value being −4gN per cow per day.

scholarly journals INTERNAL QUALITIES OF EGG(S) OF LAYING BIRDS FED DIETS CONTAINING VERNONIAAMYGDALINALEAF (BITTER LEAF) MEAL AT DIFFERENT GROWING PHASES

2021 ◽  
Vol 9 (11) ◽  
pp. 348-350
Author(s):  
Adetoro- AwopetuB.O. ◽  
◽  
Olusola O.O. ◽  
Adetola O.O. ◽  
Odesola O.A. ◽  
...  
Keyword(s):  
Shell Thickness ◽  
Shell Height ◽  
Experimental Period ◽  
Control Treatment ◽  
Phase Point ◽  
Shell Weight ◽  
Significant Difference ◽  
Experimental Treatments ◽  
Dietary Treatments ◽  
Haugh Unit

One hundred and twenty eight Isa-Brown pullet chicks were used for this study to determine the internal qualities of egg(s) from birds fed diets containing Vernoniaamygdalina leaf meals at different growing phases. The leaves used were air-dried, crushed and milled, and was then incorporated into the experimental treatments (T2, T3, and T4) at 2% inclusion each. The birds were randomly allotted into four dietary treatments (T1, T2, T3 and T4) and each treatment was replicated four times with each replicate having 8 birds from the chick phase to the growing phase, thereafter 6 birds per replicate were randomly picked at the laying phase (point of cage), with T1 serving as the control treatment throughout the experimental period of 39weeks. The parameters measured include egg weight, shell weight, shell thickness, shell height, yolk weight, yolk height, yolk width, albumin length, albumin height, albumin weight, while yolk index and Haugh unit were calculated. The result of the experiment showed that T3 with 2% inclusion of Vernoniaamygdalinafrom the growing phase had the best shell thickness, yolk color and acceptable value for Haugh unit. Other parameters measured showed that yolk weight, and Haugh unit were significant at (p<0.05) with 2% inclusion of Vernoniaamygdalina. Every other parameter measured showed no significant difference across the treatments at (p>0.05). The result showed that 2% inclusion of Vernoniaamygdalinaleaf meal at the growing phase of the experiment improved the internal qualities of eggs.

scholarly journals Hydrology and water resources in Caspian Sea

2016 ◽  
Vol 374 ◽  
pp. 113-116
Author(s):  
Kourosh Haddadi Moghaddam
Keyword(s):  
Water Resources ◽  
Caspian Sea ◽  
Stomach Contents ◽  
Flood Frequency ◽  
The Caspian Sea ◽  
Persian Sturgeon ◽  
Acipenser Persicus ◽  
Bony Fishes ◽  
Significant Difference ◽  
Hydrology And Water Resources

Abstract. Precipitation is the main driver of the water balance variability of the water over space and time, and changes in precipitation have very important implications for hydrology and water resources. Variations in precipitation over daily, seasonal, annual, and decadal time scales influence hydrological variability over time in a catchment. Flood frequency is affected by changes in the year-to-year variability in precipitation and by changes in short-term rainfall properties. Desiccation of the Caspian Sea is one of the world's most serious ecosystem catastrophes. The Persian Sturgeon (Acipenser persicus) caught under 10 m depth using bottom trawl net by research vessel during winter 2012, summer and winter 2013 and spring 2014 in east, central and west of southern parts of Caspian Sea, then, their diets were investigated. During 136 trawling in the aimed seasons, Persian sturgeon with 1 to 2 years old and 179.67 × 0.2 g (body weight) and 29.97 ± 0.4 cm (Total length) captured. Examination of stomach contents in the sturgeon specimens revealed that the food spectrum was composed of bony fishes (Neogobius sp., Atherina sp. and Clupeonella delicatula), invertebrates belonging to the family Ampharetidae polychaeta worms including (Hypanai sp. and Nereis diversicolor), various crustaceans (Gammarus sp. and Paramysis sp.). Investigation on stomach contents of sturgeon Acipenser persicus caught under 10 m depth in 2012 to 2013 surveys showed that there is significant difference in the consumed food. The most food diversity have been observed in winter 2013, also Polychaeta is the primary consumed food and crustacean is the secondary one (P > 0.05), no new types of food (such as bony fishes or benthics) have been observed on food chain of Acipenser persicus and shows no significant difference (P > 0.05).

Isolation, morphometry, and culture of Colurella sp. (Rotifera: Ploimida)

2013 ◽  
Vol 1 (2) ◽  
pp. 143
Author(s):  
Petrus P Letsoin ◽  
Henneke Pangkey ◽  
Julius Sampekalo ◽  
Inneke F.M Rumengan ◽  
Stenly Wullur ◽  
...  
Keyword(s):  
Body Size ◽  
Marine Fish ◽  
Fish Larvae ◽  
Nannochloropsis Oculata ◽  
Tropical Fish ◽  
Sampling Location ◽  
Larval Rearing ◽  
Brachionus Rotundiformis ◽  
Marine Fish Larvae ◽  
Significant Difference

The rotifer Brachionus rotundiformis (total body length 240.59±10.24 μm, lorica length 175.28±9.18 μm, and lorica width 124.28±7.76μm) is commonly used as starter food in the larval rearing of marine fish. But, larvae of some marine tropical fish species required starter food with body size smaller than B. rotundiformis. The present study was aimed to isolate minute rotifers from nature and to assess the possibility of culturing these rotifers. Sampling of rotifers was conducted in an estuary of Mangket (Kema-Minut), using plankton net (mesh size 40 µm). A trial of culturing the rotifers was conducted at salinities of 10, 20 and 30 ppt by using a microalga, Nannochloropsis oculata. A species of rotifer identified as Colurella sp. (family Lepadellidae) was successfully isolated from the sampling location. Body size of Colurella sp. was extremely small (Total length 123.22±5.45 μm, lorica length 95.96±3.81 μm, and lorica width 53.57±3.11 μm), which were smaller than Brachionus rotundiformis SS-type as a conventional starter food for marine fish larvae.  Results of culturing the minute rotifer Colurella sp. showed that the species grew well at salinities of 10, 20 and 30 ppt with no significant difference among treatments (ANOVA, p>0.05), indicating a potential use of minute rotifer Colurellasp. as starter food for marine fish larvae. Rotifera Branchionus rotundiformis (ukuran tubuh: panjang total 240,59±10,24 μm, panjang lorika 175,28±9,18 μm, dan lebar lorika 124,28±7,76μm) sering digunakan sebagai pakan awal pemeliharaan larva ikan laut. Namun, larva beberapa spesis ikan laut tropis membutuhkan pakan awal berukuran tubuh lebih kecil dari Branchionus rotundiformis. Penelitian ini bertujuan untuk mendapatkan minute rotifer dari alam (berukuran tubuh lebih kecil dari B. rotundiformis) dan menguji kemungkinan pemeliharaannya. Sampling rotifer dilakukan di perairan estuari Desa Mangket (Kema-Minut), menggunakan plankton net (ukuran mata jaring 40 µm). Uji coba pemeliharaan dilakukan pada salinitas (10, 20, dan 30 ppt) dengan menggunakan Nannochloropsis oculata. Satu spesies minute rotifer yang teridentifikasi sebagai Colurella sp. (family Lepadellidae) berhasil diisolasi dari lokasi sampling. Colurella sp. memiliki ukuran tubuh sangat kecil (panjang total [PT] 123,22±5,45 µm, panjang lorika [PL] 95,96±3,81 µm, dan lebar lorik [LL] 53,57±3,11 µm) yang mana lebih kecil dari Branchionus rotundiformis tipe-SS sebagai pakan awal larva ikan laut. Hasil uji coba pemeliharaan minute rotifer Colurella sp. menunjukkan bahwa spesis ini dapat tumbuh pada salinitas 10, 20, dan 30 ppt dengan perbedaan kepadatan populasi yang tidak signifikan antar perlakuan (Uji ANOVA, p > 0.05) mengindikasikan potensi pemanfaatan minute rotifer Colurella sp. sebagai pakan awal larva ikan laut.

scholarly journals The Effectiveness of Candidate Probiotic Bacteria to Control Vibriosis Disease

2017 ◽  
Vol 5 (2) ◽  
pp. 1
Author(s):  
Mulyati Mulyati ◽  
Suryati Suryati ◽  
Irfani Baga
Keyword(s):  
Survival Rate ◽  
Sea Water ◽  
Challenge Test ◽  
Probiotic Bacteria ◽  
Pathogenicity Test ◽  
Inhibitory Ability ◽  
Significant Difference ◽  
Portunid Crab

The study aims to isolate, characterize, and examine probiotic bacteria's inhibitory ability against Vibrio harveyi bacteria, both in-vitro and in vivo. Methods used in the study consist of 1) An Isolation of Candidate Probiotic Bacteria, 2) An Antagonistic Test of Candidate Probiotic Bacteria in vitro, 3) An Identification of Bacteria, 4) A Pathogenicity Test of Candidate Probiotic Bacteria, 5) An Antagonistic Test of Candidate Probiotic Bacteria against V. harveyi in vivo. According to the isolation of candidate probiotic bacteria, there are 18 isolated candidate probiotic. After being tested for its inhibitory ability in vitro, there are 8 isolates with zone of inhibition as follows: isolate MM 7 from intestine (22 mm), isolate MM 6 from intestine (12 mm), isolate MM 10 from sea water (10 mm), isolate MM 5 from intestine (9 mm), isolate MM 4 from intestine (8 mm), isolate MM 3 from intestine (7 mm), isolate MM 2.2 from intestine (7 mm), isolate MM 2.1 from intestine (7 mm). Eight genera of the candidate probiotic bacteria is derived from Portunid crab, they are Staphylococcus, Streptococcus, bacillus, vibrio, Alcaligenes, Lactobacillus, micrococcus. Before proceeding the V. harveyi bacterial challenge test in vivo, three potential isolates consisting of MM6, MM7 and MM10 as the probiotic bacteria are pathogenicity-tested against V. harveyi. The survival rate of Portunid crab on pathogenicity test using MM6, MM7 and MM10 generates 91.11-100%, while the control generates 100% survival rate. Variance analysis result through post-hoc Tukey's Honest Significant Difference (HSD) test at 95% confidence interval indicates that isolate MM7 and MM10 are significantly able to increase hatchling Portunid crab's survival rate.

UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) PADA MENCIT PUTIH JANTAN (Mus musculus) DENGAN INDUKSI NYERI ASAM ASETAT

2017 ◽  
Vol 2 (2) ◽  
pp. 147
Author(s):  
Triswanto Sentat ◽  
Susiyanto Pangestu
Keyword(s):  
Analgesic Effect ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Treatment ◽  
Treatment Groups ◽  
Analgesic Effects ◽  
Significant Difference ◽  
Analgesic Dose ◽  
Muntingia Calabura

Kersen leaf (Muntingia calabura L.) contains tannins, flavonoids and polyphenol compounds allegedly have analgesic effect. The objective was to determine the analgesic effect of ethanol extract of kersen leaves and to determine the most effective analgesic dose. This study was an experimental research. Leaves were extracted with ethanol 70% and the analgesic effect test was divided into 5 groups: negative control treatment (distilled water), positive control (mefenamic acid 2.6mg/kg), kersen leaf ethanol extract first dose (100mg/kg), second dose (200mg/kg) and tthird dose (400mg/kg). Giving treatments by oral, after 30 minutes, the mices were given a pain inductor with 0.5% acetic acid by intra peritonial administration. Analgesic power was calculated by counting the number of writhing in mice for 1 hour. The results showed that the ethanol extract of cherry leaf has analgesic effect. From the calculation of the first dose analgesic power (42.9%), second dose (59.4%) and the third dose 69.9%. Statistical test results kruskal wallis value of p=0.011 (p<0.05) showed a significant difference between all analgesic treatment groups. The conclusion of this study is all of the ethanol extract had analgesic effects on male white mice, whereas a dose of 400mg/kg is the most effective analgesic dose.

scholarly journals Effects of Insecticides for Controlling Hogplum Beetle Podontia 14-punctata under Laboratory and Field Conditions

2015 ◽  
Vol 8 (1) ◽  
pp. 79-83
Author(s):  
MJ Uddin ◽  
MMH Khan
Keyword(s):  
Natural Resources ◽  
Field Condition ◽  
Field Trial ◽  
Adult Stage ◽  
Control Treatment ◽  
Larval Instars ◽  
Significant Difference ◽  
Laboratory Trial ◽  
The Mean ◽  
Hog Plum

Experiments on hogplum beetle, Podontia 14-punctata were carried out to find out the suitable insecticide(s) with appropriate dose(s) for suppressing both larvae and adults. Results revealed that the highest mortality (100%) was found in 1st instar larvae after 24 hours at each dose of superior 505EC and the lowest mortality was in T4 treated leaves. The highest mortality was also found in 2nd, 3rd, 4th and adult stages of hog-plum beetle by T5 and T6 treatments. No mortality was observed in control treatment in laboratory trial. The mean number of larvae ranged from 1.5 to 7.6 per square meter of quadrate at one week after last spray. No significant difference was observed among treatments regarding percent leaf infestation at one day before 1st spray. The percent leaf infestation ranged from 5.01 to 9.82 at one week after last spray. The lowest percent (5.87%) leaf infestation was found in T6 treated plants while the highest percent (15.76%) leaf infestation was found in untreated control plants. The highest mortality was observed in T5 and T6 treated plants at all larval instars and no significant difference was found between two doses of each insecticide under field condition. The results of field trial also revealed that the minimum dose (0.75 ml L-1 of water) of Superior 505 EC is sufficient to suppress the field population of larvae and adults of Podontia 14-punctata effectively. Finally, it may be concluded that the highest mortality was found in all larval instars and adult stage of hog-plum beetle after 24 hours at each dose of superior 505 EC (T5 and T6 treatments) both in laboratory and field trials.J. Environ. Sci. & Natural Resources, 8(1): 79-83 2015

scholarly journals Study of indole butyric acid (IBA) effects on cutting rooting improving some of wild genotypes of damask roses (Rosa damascena Mill.)

2015 ◽  
Vol 60 (3) ◽  
pp. 263-275 ◽  
Author(s):  
Fardin Nasri ◽  
Arsalan Fadakar ◽  
Mahmood Saba ◽  
Bayzid Yousefi
Keyword(s):  
Butyric Acid ◽  
Control Treatment ◽  
Rosa Damascena ◽  
Indole Butyric Acid ◽  
Significant Difference ◽  
Maximum Root Length ◽  
Number Of Leaves ◽  
Maximum Root ◽  
Medicinal Properties ◽  
The Rose

Rosa damascena is very important for essential oil production, medicinal properties and it is also widely cultivated as a garden rose. The Rose species is mainly propagated by stem cutting. In the present study, the effect of different levels of 0, 500 and 1,000 mg l-1 (quick dip method for 20 s) of indole butyric acid (IBA) on the rooting of 12 wild genotypes (including: Kurdistan 1 to Kurdistan 12) of R. damascena was investigated. The results show that the rooting ability of R. damascena differs significantly between the twelve genotypes. The highest rooting (79.56%) and callus production (69.08%), number of roots (8.33), root fresh and dry weights (361.80 and 244.74 mg, respectively) were recorded in Kurdistan 5 genotype with 1,000 mg l-1 IBA. The maximum root length (5.84 cm) was observed in Kurdistan 5 genotype with 500 mg l-1 IBA that showed a significant difference compared to the control treatment (0.96 cm). The highest number of leaves per bud (7.33 at 500 mg l-1 IBA) and number of buds (5.00 at 1,000 mg l-1 IBA) were recorded in Kurdistan 1 genotype. The current study demonstrated that the different genotypes of R. damascena were in a difficult-toroot state, which suggests that cutting treatment with 1,000 mg l-1 IBA overcame the problem of the difficult-to-root state, and it can also enhance the rooting percentage in the studied genotypes.

Sign in / Sign up

Export Citation Format

Share Document