Clinical and Diagnostic Value of Cardiac Markers in Human Biological Fluids

Kardiologiia ◽  
2019 ◽  
Vol 59 (11) ◽  
pp. 66-75 ◽  
Author(s):  
A. M. Chaulin ◽  
L. S. Karslyan ◽  
E. V. Bazyuk ◽  
D. A. Nurbaltaeva ◽  
D. V. Duplyakov
Keyword(s):  
Blood Serum ◽  
Oral Fluid ◽  
Biological Fluids ◽  
High Sensitivity ◽  
Diagnostic Value ◽  
Cardiac Markers ◽  
Methods Of Analysis ◽  
Clinical Diagnostic

The article is devoted to problems of clinical-diagnostic value of determination of cardio-specific troponins in human biological fluids. Improvement of laboratory instrumentation and emergence of high sensitivity methods of analysis have allowed to identify troponins in urine, dialysate, and oral fluid. In the review we present actual information related to measurement of troponins in blood serum, data on testing of cardio-specific troponins in urine, dialysate, and oral fluid. Special attention is paid to determination of some cardiomarkers in oral fluid with thorough analysis of diagnostic value and effectiveness of the conducted studies.

Emission Spectrometric Determination of Trace Elements in Biological Fluids

1971 ◽  
Vol 25 (1) ◽  
pp. 53-56 ◽  
Author(s):  
William Niedermeier ◽  
James H. Griggs ◽  
Richard S. Johnson
Keyword(s):  
Experimental Data ◽  
Trace Elements ◽  
Sample Preparation ◽  
Blood Serum ◽  
Biological Fluids ◽  
Spectrometric Method ◽  
Direct Reading ◽  
Iron Aluminum ◽  
Dc Arc

An emission spectrometric method of analysis is described, in which trace quantities of copper, iron, aluminum, barium, manganese, nickel, cesium, tin, strontium, chromium, zinc, lead, molybdenum, and cadmium were determined in blood serum. The sample preparation, starting with 2.0 ml of blood serum, is discussed in detail. The source of excitation was a 10 A dc arc. Quantitation was achieved with a direct reading emission spectrometer. The metal concentration, in micrograms per 100 ml of blood serum, was calculated from the experimental data by means of a computer.

High sensitivity ELISA determination of taxol in various human biological fluids

1999 ◽  
Vol 20 (3) ◽  
pp. 549-555 ◽  
Author(s):  
Stan R Svojanovsky ◽  
Kamal L Egodage ◽  
Jun Wu ◽  
Milan Slavik ◽  
George S Wilson
Keyword(s):  
Biological Fluids ◽  
High Sensitivity

scholarly journals HEMATOSALIVAL BARRIER IN CLINICAL PRACTICE

2021 ◽  
Vol 17 (2) ◽  
pp. 62-67
Author(s):  
Oleg Chuykin ◽  
Maria Shtanko ◽  
Kristina Kuchuk
Keyword(s):  
Oral Fluid ◽  
Biological Fluids ◽  
The Body ◽  
Internal Environment ◽  
Pathological Conditions ◽  
Therapeutic Measures ◽  
The Difference ◽  
And Control ◽  
Level Analysis

To maintain homeostasis of the internal environment of the body, mechanisms are needed that control the exchange of various components between blood and the internal environment of tissues and organs. One of these physiological structures is the blood-salivary barrier, which regulates the selective metabolism between blood and oral fluid. Changes in the composition of biological fluids (blood and saliva) can be used as markers for diagnosing pathological conditions of the body and the dynamics of therapeutic measures. Of great importance is the study of the blood-salivary barrier for the search for drugs that are selectively transferred from the blood to the saliva. Some drugs are excreted in saliva at a concentration higher than they are in the blood. However, more often the concentration of drugs in saliva is much lower than in blood serum. Purpose: to form a list of the main trends and scientific and practical directions on the func-tioning of the hematosalvarial barrier. Materials and methods: to analyze the topic of the clinical significance of the hematosalvarial barrier, periodicals and scientific journals of domestic and foreign literature were studied, which determined the difference in the levels of trace elements, hormones, immune complexes between the oral fluid and serum. Conclusion: Analysis of literature data shows that the function of the blood-salivary barrier acts as a nonspecific adaptive mechanism, while saliva reflects the state of the protective functions of the barrier at the organismal level. Analysis of the selectivity and permeability of the blood-salivary barrier, as well as determination of changes in the composition and proper-ties of blood and oral fluid, can optimize and facilitate the diagnosis and control of treatment of various diseases of the oral cavity and the body as a whole.

scholarly journals Salivary biomarkers and proteomics: future diagnostic and clinical utilities

2021 ◽  
Vol 19 (3) ◽  
pp. 171-174
Author(s):  
A. V. Mitronin ◽  
O. A. Khvorostenko ◽  
D. A. Ostanina ◽  
Yu. A. Mitronin
Keyword(s):  
Biochemical Parameters ◽  
Oral Fluid ◽  
Biological Fluids ◽  
Inorganic Compounds ◽  
Non Invasive ◽  
Fluid Analysis ◽  
Methods Of Analysis ◽  
Wide Range ◽  
Biological Environment ◽  
Physiological And Biochemical

The search for new, fast and non-invasive methods of diagnosing diseases of both the oral cavity and general diseases of various etiologies and their introduction into practical health care is still a priority in the field of medicine. Among the known methods of analysis of biological fluids, a special place is occupied by the study of saliva. Oral fluid analysis has a high potential in screening for various diseases, since it contains a wide range of organic and inorganic compounds. A significant number of works have been devoted to the study of the quantitative and qualitative composition of the oral fluid, as well as to the study of saliva biomarkers, however, the study of the saliva proteome is at the stage of data accumulation. The lack of standardization in the collection of samples and methods of analysis, as well as poorly studied physiological and biochemical parameters of the oral fluid, hinders the introduction of advances in the study of the saliva proteome into diagnostic practice. The solution of these problems will allow the oral fluid to be used as a biological environment for both detecting diseases and predicting their course.

scholarly journals Development of Human Serum Albumin Selective Fluorescent Probe Using Thieno[3,2-b]pyridine-5(4H)-one Fluorophore Derivatives

Sensors ◽  
2019 ◽  
Vol 19 (23) ◽  
pp. 5298 ◽  
Author(s):  
Lee ◽  
Sung ◽  
Kang ◽  
Parameswaran ◽  
Choi ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
High Throughput Screening ◽  
Biological Fluids ◽  
Clinical Importance ◽  
High Sensitivity ◽  
Fold Increase ◽  
Artificial Urine

The level of human serum albumin (HSA) in biological fluids is a key health indicator and its quantitative determination has great clinical importance. In this study, we developed a selective and sensitive fluorescent HSA probe by fluorescence-based high-throughput screening of a set of fluorescent thieno[3,2-b]pyridine-5(4H)-one derivatives against major plasma proteins: HSA, bovine serum albumin (BSA), globulin, fibrinogen, and transferrin. The fluorophore chosen finally (4 in Scheme 1) showed noticeable fluorescence enhancement in the presence of HSA (160-fold increase), and it exhibited rapid response, high sensitivity (detection limit 8 nM), and the ability to clearly distinguish HSA from BSA in pH 9 buffer condition. Moreover, the probe could be applicable to detect trace amounts of HSA in an artificial urine sample; further, it might be applied to the determination of the HSA concentration in complex biological samples for pre-clinical diagnosis.

scholarly journals New Approach for Specific Determination of Antibiotics by Use of Luminescent Escherichia coli and Immune Serum

2004 ◽  
Vol 70 (2) ◽  
pp. 1245-1248 ◽  
Author(s):  
Irina I. Vlasova ◽  
Tatyana V. Asrieli ◽  
Elisaveta M. Gavrilova ◽  
Vadim S. Danilov
Keyword(s):  
Escherichia Coli ◽  
Blood Serum ◽  
High Sensitivity ◽  
High Specificity ◽  
Immune Serum ◽  
Genetically Engineered ◽  
New Approach ◽  
System Sensitivity ◽  
E Coli

ABSTRACT This paper describes a possible application of luminescent Escherichia coli activated by blood serum for high-sensitivity and high-specificity assays of antibiotics in solutions. Antibiotics inhibited luminescence of a genetically engineered E. coli strain; the system sensitivity to some antibiotics grew notably after the cells had been preactivated by blood serum. The highest level of sensitivity (2.8 ± 0.6 ng/ml) of luminescent cells was obtained for aminoglycoside antibiotics (gentamicin and streptomycin). It is feasible to create the specific biosensor for antibiotics on the basis of bioluminescent E. coli strains by applying sera containing antibodies against the antibiotic under assay. The presence of antibodies specific for gentamicin in serum affects inhibition of luminescent cells by gentamicin but not inhibition by other antibiotics.

scholarly journals Endogenous Lactoferrin in the Assessment of Ferrokinetics in Children with Iron Deficiency States

2019 ◽  
pp. 44-50
Author(s):  
N. N. Klimkovich ◽  
G. P. Zubritskaya ◽  
E. I. Venskaya ◽  
A. G. Kutko ◽  
A. S. Skorobogatova ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Blood Serum ◽  
Biological Fluids ◽  
Laboratory Data ◽  
Deficiency Anemia ◽  
Control Group ◽  
Healthy Children ◽  
Physiological Mechanisms ◽  
Latent Iron Deficiency

Objective: to study the concentration of endogenous lactoferrin in children with iron deficiency states before and during the treatment. Material and methods . The clinical and laboratory data of 31 patients with iron deficiency states (24 patients with iron deficiency anemia and 7 with latent iron deficiency) aged 0-17 (the median age was 9.7) and 7 healthy children (the median age was 12.4) who made up the control group have been analyzed. Hemogram and biochemical blood test were done according to the standard method. The concentration of endogenous LF in the blood serum was determined by the method of enzyme immunoassay using commercial test systems for the quantitative determination of human LF in the biological fluids (Elabscience) on the universal VICTOR2TM analyzer (Perkin Elmer, USA). Results . The study of ferrokinetics and LF concentration in the blood serum of the children with iron deficiency states has been performed. It has been shown that the children with iron deficiency reveal an increased LF level in the blood serum, which has variable values depending on age. The highest levels of the serum LF content are characteristic of patients under the age of three months, which is associated with the physiological mechanisms of the adaptation process of the hematopoietic system in newborns and infants. The LF concentration in the blood serum associated with ferrotherapy is increased compared to the corresponding indices before the treatment. Conclusion . From the results of the study its follows that the content of LF in the blood serum increases in iron deficiency and is associated with ferrotherapy. The obtained results will be used in further research aimed at the diagnosis of iron deficiency and prediction of the response to iron therapy.

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